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Seat No.: Enrolment No.
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GUJARAT TECHNOLOGICAL UNIVERSITY
B.PHARM - SEMESTER - 6- EXAMINATION -WINTER - 2018
Subject Code:2260002 Date: 27/11/2018
Subject Name: Pharmaceutical Microbiology & Biotechnology - I1
Time:02:30 PM TO 05:30 PM Total Marks: 80
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Instructions:
1. Attempt any five questions.
2. Make Suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
Q.1 (a) Explain the different parts of a Fermentor with their functions with a labeled diagram. 06
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(b) Write a note on components of a fermentation media. 05(c) Explain in brief the production of streptomycin by fermentation process 05
Q.2 (a) Describe the primary defensive mechanisms of the body in brief. 06
(b) Explain the process of making BCG vaccine with a flow diagram 05
(c) Define and classify Immunity with suitable examples 05
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Q.3 (a) Describe the production of monoclonal antibodies by hybridoma technology 06
(b) Describe techniques of protoplast fusion. 05
(c) Describe in brief mechanisms of Gene Transfer 05
Q.4 (a) Discuss the principle and method of microbiological assay of antibiotics by cup plate method 06
(b) Differentiate between: (1) Active and passive Immunity (ii) Vaccine and Sera 05
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(c) Describe the structure of an antibody. Classify types of antibodies along with their function. 05Q.5 (a) Describe the process of recombinant DNA technology with a suitable diagram. What are restriction enzymes? 06
(b) Describe the production of Humulin( Human Insulin) by recombinant DNA technology 05
(c) Write a note on Vectors used in recombinant DNA technology 05
Q.6 (a) Define Mutation. Classify and define types of mutation. 06
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(b) Discuss the process of collection and storage of whole human blood 05(c) Discuss causes of mutation. 05
Q.7 (a) Explain the principle and method of sterility testing. Which Pharmaceuticals are required to be tested for sterility? 06
(b) Write a note on Dried Human Plasma. 05
(c) Describe in brief Antigen antibody reactions 05
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