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Download MBBS Biochemistry PPT 47 Final Carbohydrate Chemistry Lecture Notes

Download MBBS (Bachelor of Medicine, Bachelor of Surgery) 1st year (First Year) Biochemistry ppt lectures Topic 47 Final Carbohydrate Chemistry Notes. - biochemistry notes pdf, biochemistry mbbs 1st year notes pdf, biochemistry mbbs notes pdf, biochemistry lecture notes, paramedical biochemistry notes, medical biochemistry pdf, biochemistry lecture notes 2022 ppt, biochemistry pdf.

This post was last modified on 05 April 2022

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Functions

of

Carbohydrates

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SYNOPSIS

Introduction
Definition of Carbohydrates

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Classification of Carbohydrates
Study of Biomedically Important

Carbohydrates:

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Monosaccharides
Disaccharides
Polysaccharides
Mucoproteins and Glycoproteins.
Biomedical Importance of Carbohydrates.

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Introduction

What are Carbohydrates?

Carbohydrates are organic

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biomolecules abundantly present in

the nature.

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Found in the cells of plants and

animals.

The term "Carbohydrate" was coined

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by "Karl Schmidt".
Carbohydrates Biosynthesis

Carbohydrates are predominantly

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biosynthesized by plants through

photosynthesis.

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Glucose is synthesized in plants

from CO2, H2O, and solar energy

from the sun.

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Photosynthesis


chlorophyll

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6 CO2 + 6 H2O

C6H12O6 + 6 O2

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Sunlight Glucose

(Simple Carbohydrate)


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(+)-Glucose Starch or Cellulose

(Complex Carbohydrates Of Plants)

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Animals and Human beings

cannot biosynthesize

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Carbohydrates

predominantly.
To fulfill metabolic and

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structural role in human

beings,

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It is essential to ingest

carbohydrates through

food substances of plant and

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animal origin.

Thus Carbohydrates are chief

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constituents of human food.

R.D.A for Dietary Carbohydrates=

400-600 gm/day.

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However in a critical

condition when cells are

deprived of Glucose

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Human body biosynthesizes

Glucose using the non

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carbohydrate precursors

present in body via

Gluconeogenesis.

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Functions of Carbohydrates

Carbohydrates serve as

primary source of

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energy/Fuel of body

( Metabolic role).

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Carbohydrate (Glucose)

is oxidized in living cells

of human body to

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produce CO2, H2O, and

energy(ATP).

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Carbohydrates provide

skeletal framework to cells

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,tissues, and organs of

body.(Structural role)

Carbohydrates are associated

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to many other roles with

human beings.

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DEFINITION OF

CARBOHYDRATES
Old Definition of Carbohydrates

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Empirical formula/General formula for

simple carbohydrates : Cn (H2O)n

Where n = number of carbon atom

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present in carbohydrate structure.

Old Definition-
Carbohydrates are "Hydrates of

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Carbon"

Old definition is not valid since-
Certain Carbohydrates ?

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Rhamnose did not fit in the

empirical formula of carbohydrates.

Certain non Carbohydrates ?

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Lactate and Acetate fitted in the

empirical formulae.
Observe the following

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chemical structures of

simple Carbohydrates:

Glucose and Fructose

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Aldose

Ketose

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(e.g., Glucose) have

(e.g., Fructose) have

an aldehyde group at a ketone group,

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one end.

usually at C2.
Simple Carbohydrates has many

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Hydroxyl groups (Polyhydroxy).

Simple Carbohydrates has carbonyl/

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functional groups as Aldehyde or

Ketone.

Simple Carbohydrates(Glucose/Fructose)

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repeatedly linked to form its condensed

complex carbohydrates for ex Starch,

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Inulin.

The hydroxyl groups may be free

or substituted by any other

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groups.

Simple Carbohydrates on

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chemical reactions produces

derivatives of Carbohydrates.
New Definition of Carbohydrates

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Carbohydrates are organic

biomolecules, abundantly

present in the plant and animal

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bodies, chemically composed of

Polyhydroxy Aldehyde or

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Polyhydroxy Ketone, their

condensed products or their

derivatives.

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Classification Of Carbohydrates

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Depending Upon Number of Saccharide Units

Four Main Classes of Carbohydrates

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Monosaccharides

(1 Saccharide Unit)

Disaccharides

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(2 Saccharide Units)

Oligosaccharides

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( 3-10 Saccharide Units)

Polysaccharides

( More than 10 Saccharide Units)

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Monosaccharides Sub Classification

Monosaccharides are sub

classified on the basis of:

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Functional Group
Number of Carbon atoms.

Number of

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Aldoses

Ketoses

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Carbon Atoms

(Aldehyde-CHO)

(Ketone -C=O)

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3

Aldo Triose

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Keto Triose

Triose

Glyceraldehyde

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Di HydroxyAcetone

4

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Aldo Tetrose

Keto Tetrulose

Tetrose

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Erythrose

Erythrulose

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5

Aldo Pentose

Keto Pentulose

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Pentose

Ribose, Xylose, Arabinose

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Ribulose, Xylulose

6

Aldo Hexose

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Keto Hexose

Hexose

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Glucose, Galactose ,Mannose

Fructose

7

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Aldo Heptose

Keto Heptulose

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Heptose

SedoHeptose

SedoHeptulose

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Disaccharides

Disaccharides has 2

Monosaccharide units

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linked by glycosidic bond.

Disaccharides may be

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reducing or non reducing

Type Of Disaccharides

Reducing Disaccharides ?

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Lactose (Glu-Gal)
Maltose (Glu-Glu)

Non reducing Disaccharides-

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Sucrose(Glu-Fru)
Oligosaccharides Sub Classification

Oligosaccharides has 3-10

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Monosaccharide units linked

by glycosidic bonds.

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Oligosaccharides are sub

classified on the basis of

number of Saccharide units.

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Number of

Type Of Oligosaccharides

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Monosaccharide

(3-10 Monosaccharide Units )

Units

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3

Trisaccharides

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Maltotriose (Glu-Glu-Glu)

Raffinose (Glu-Fru-Gal)

4

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Tetrasaccharides

Stachyose (Glu-Fru-2Gal)

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5

Pentasaccharides

Verbascose (Glu-Fru-3Gal)

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Polysaccharide Sub Classification

Polysaccharides/ Glycans
contain more than 10, same/

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different Monosaccharide

units linked by glycosidic

linkages.

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Types of Polysaccharides

Homopolysaccharides/

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Homoglycans-

Contains more than 10 same repeating

units.

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Heteropolysaccharides /

Heteroglycans-

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Contains more than 10, different

repeating units.
POLYSACCHARIDES/ Glycans

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( More than 10 Monosaccharide Units )

Homopolysaccharides/ Homoglycans

( > 10 Same Repeating Units )

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Glucosans

Fructosans

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(Repeating Unit of Glucose/Polymer (Repeating Unit of

of Glucose)

Fructose/Polymer of

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Starch

Fructose)

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Glycogen

Cellulose

Inulin

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Dextrin

Dextran

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Hetero Polysaccharides

( More than 10 Different

Repeating Units )

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Animal

Heteropolysacchrides

Mucopolysaccharides (MPS)

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OR

Glycosaminoglycans (GAGs)

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Types And Examples of

Mucopolysaccharides
Acidic Non Sulfated MPS:

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Hyaluronic Acid

Acidic Sulfated MPS:

Heparin

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Heparan Sulfate

Chondritin Sulfate

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Dermatan Sulfate

Keratan Sulfate

Neutral MPS:

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Blood Group Substances

Plant Heteropolysaccharides

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Agar
Pectin
Lignin
Gum
What are Sugars?

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Sugars are chemically simple

Carbohydrates Monosaccharides and

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Disaccharides.

Sugars are Crystalline Solid substances.
Soluble in water
Sweet in taste

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Structure possess asymmetric /chiral

carbon atoms/stereogenic centers.

The carbonyl/

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functional groups of

Carbohydrates may be

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present as free or

bound (involved in

glycosidic bonds).

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Types Of Sugars

Reducing Sugars
Non Reducing

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Sugars

Reducing Sugar

Sugar structure possessing free or

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potential(reactive) aldehyde or

ketone group is termed as

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reducing sugar.

Reducing sugars show reducing

property efficiently in alkaline

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medium and reduces certain

metallic ions as- Cu++;Bi++;Fe+++
Reducing Sugars answer following

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tests positive

Benedict's Test
Fehling's test

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Nylander's Test
Form Osazones.
Reducing Shows Mutarotation

(Change in Optical activity)

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Examples Of Reducing Sugars

All Monosaccharides are reducing sugars.
Monosaccharides are strong reducing

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agents.

Monosaccharides?

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Ribose, Glucose, Galactose, Fructose.

Disaccharides are weak reducing agents.
Reducing Disaccharides-

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Lactose, Maltose.
Non Reducing Sugars

Sugar structure not possessing

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free or potential aldehyde or

ketone group in its structure is

termed as non reducing sugar.

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Non reducing sugar does not

show reducing property and do

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not reduce metallic ions.

Non reducing sugars give following

reducing tests negative.

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Benedict's Test
Fehling's test
Nylander's Test
Do not form Osazones

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Non Reducing sugars do not exhibit

Mutarotation (Change in Optical activity)
Examples of Non reducing Sugars

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Non reducing Disaccharides.

Sucrose (Biomedically Important)
Trehalose (Glu-Glu linked with

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(1-1) glycosidic bond)

Polysaccharides/Complex

Carbohydrates are Non reducing.

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Sugar/Sugar Derivatives

Percent

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Sweetness

Glucose

75

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Fructose

175

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(Sweetest Sugar)

(Highest)

Galactose

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30

Sucrose

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100

Lactose

20

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Maltose

30

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Xylitol

250
Non Carbohydrate Percent Sweetness

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Synthetic Sweetners

Saccharin

45,000 times

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Aspartame

18,000 times

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(Asp-Phe)

Thaumatin and

10,000 times

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Monellin

Cyclamate

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1000 times

Biomedical Importance of Sugars

Sugars are sweetening

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agents used in

preparations of fruit

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juices, sweet recipes

which gives delicious taste.
Sugars have dietary and

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calorific value.

Ingested sugars are digested

,absorbed and assimilated to

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produce chemical form of

energy ?ATP, which is further

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used for body activities.

Carbohydrates are

Optically Active and

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Show Stereoisomerism
All Carbohydrates except Di Hydroxy

Acetone(DHA) possess asymmetric

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carbon atoms in their structure.

Presence of Asymmetric carbon atoms

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confer two properties:

Optical Activity
Stereoisomerism.

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Optical Activity

Optically active solutions when

placed in the tube of Polarimeter.

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If moves the plane of polarized

light toward right are dextro

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rotatory (d/+).

If moves the plane of polarized

light toward left are laevo rotatory

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(l/-).
Stereoisomerism

Stereoisomerism is

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due to presence of

chiral carbon

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atoms/stereogenic

centers.
Stereoisomers are type of

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isomers

Which have same chemical and

molecular formula,

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The structure slightly differs in

the spatial orientation of

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groups around the carbon atom.

Biomedically Important

Monosaccharides

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Monosaccharides (Simple Sugars)
Monosaccharides are simplest class

of Carbohydrates.

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They are composed of one

saccharide unit.

Monosaccharides cannot be further

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hydrolyzed.

Monosaccharides are building

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blocks/monomeric units of higher

forms of Carbohydrates.

Glyceraldehyde/Glycerose

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Simplest Carbohydrate

(Reference sugar)
Glyceraldehyde is

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a Monosaccharide

Chemically ?

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Aldo Triose

C3H6O3

Occurrence/Sources of Glyceraldehyde

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In Cytoplasm of cells
Biomedical Importance's

Glyceraldehyde -3-Phosphate

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is an intermediate of

Glycolysis and HMP shunt.

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Glyceraldehyde is reduced

to Glycerol which is used

during Lipid and Glucose

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biosynthesis.

DihydroxyAcetone

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It is a Monosaccharide

DHA is a Functional

Isomer of Glyceraldehyde.

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Chemistry Of DHA

Dihydroxy Acetone is a

Keto Triose.

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C3H6O3
DHA has no chiral atom

in its structure.

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Occurrence/Sources Of DHA

In Cytoplasm of Cells

Biomedical Importance's Of DHA

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Dihydroxy Acetone

Phosphate(DHAP) is an

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intermediate of Glycolysis.

DHAP is readily

interconvertable to

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Glyceraldehyde -3-PO4.
Erythrose

Chemistry :

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Erythrose is a Monosaccharide

Erythrose is an Aldo Tetrose

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C4(H2O)4
Occurrence/Sources :
In Cytosol of cells
Biomedical Importances :
Erythrose -4-Phosphate is an

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intermediate of HMP shunt.

Ribose

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Chemistry :

Ribose is a Monosaccharide.
Ribose is an Aldo Pentose
C5(H2O)5

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Occurrence/Sources :

In cells

Biomedical Importances of Ribose :

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Ribose is an important component of

Ribonucleotides which forms RNA.

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Ribose is component of certain Nucleotide

Coenzymes-

ATP, NAD+, NADP+,FAD

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Deoxyribose

Deoxyribose is a

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Monosaccharide

Derived from Ribose/

Derivative of Ribose

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Chemistry :

Deoxyribose is a Aldo Pentose
Deoxyribose has one Oxygen atom

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less than Ribose at C2.

Deoxyribose has no ?OH group at

C2

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Instead has ?H at C2.
Occurrence/Sources :
In cells
Biomedical Importances Of

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Deoxyribose :

Deoxyribose is a component of

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Deoxyribonucleotides which

forms DNA.

Arabinose

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Arabinose is a

Monosaccharide

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Chemistry :
Arabinose is a Aldo Pentose
C5(H2O)5
Occurrence/Sources:
Gum Arabic and Cherries.

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Biomedical Importances
Arabinose is a component

of Glycoproteins.
Xylose

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Xylose is a Monosaccharide

Chemistry :
Xylose is an Aldo Pentose

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C5(H2O)5
Occurrence/ Sources :
Wood Gum
Biomedical Importances :

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Xylose is a component of

Glycoproteins and Proteoglycans

Xylulose

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Xylulose is a

Monosaccharide

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Chemistry :
Xylulose is a Keto Pentose

C5(H2O)5
Occurrence/ Sources :

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In Cells
Biomedical Importances of Xylulose:
Xylulose -5-Phosphate is an

intermediate of HMP Shunt.

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Xylitol reduced compound of Xylulose

is used as sweetener (250% Sweetness).
Ribulose

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Chemistry:

Ribulose is a

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Monosaccharide

Ribulose is a Keto

Pentose

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C5(H2O)5

Occurrence/ Sources :
In Cytosol of cells.

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Biomedical Importances

of Ribulose:

Ribulose-5-Phosphate occurs

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as an intermediate of HMP

Shunt.
Glucose

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Grape sugar
Chief blood sugar
Main sugar of body cells.
Also termed as Dextrose

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Chemistry of Glucose

Glucose Chemically ?
Aldo Hexose.

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Molecular Formula-
C6H12O6
C1 is an Anomeric

carbon of Glucose.

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C1 has

carbonyl/Functional

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group.

Structures Of Glucose
Cyclic forms for sugars

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Fischer projections for a D Glucose

7P1-95

Fischer's and Haworth's Projection

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Fischer's Projection

Cyclization of Glucose to hemiacetal is

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spontaneous to form stable ring structures.

Haworth's Projection

Gluco Furanose -5 membered ring with Oxygen

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atom in it.

Gluco Pyranose -6 membered ring with Oxygen

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atom in it.
Monosaccharides can cyclize to

form Pyranose / Furanose forms

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b =64%

a = 36%
Chair Conformation Of Glucose

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Blood Glucose is more

thermodynamically stable in

D Glucopyranose form.

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Occurrence/Sources of Glucose

Glucose is found in free or bound

state in nature.

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Glucose is a component of

Disaccharides and Polysaccharides.

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Glucose found-In fruits, human

blood and body cells.

Physical properties of Glucose

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Glucose possess asymmetric/chiral

carbon atoms in its structure, this

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confers 2 physical properties:

Optical Activity
Stereoisomerism
Optical Activity of Glucose

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Optical activity for an aqueous

solution of Glucose is

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dextrorotatory(+/d)

It rotates the plane of plain

polarized light in Polarimeter

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towards right.

Hence Glucose is also termed

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as Dextrose.

Specific rotation of Glucose

optical activity:

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Pure -D Glucose = specific rotation

+112.2 ?

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Pure -D Glucose = specific rotation

+18.7 ?


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Isomers of Glucose

Cyclic structure of Glucose posses 5

asymmetric carbon atoms.

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The number of isomers is 2n, where n is

the number of asymmetric centers.

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According to Vant Hoff rule 25
Glucose posses 32 possible Isomers.

Functional / Structural Isomers of

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Glucose

Glucose (Aldo Hexose) and

Fructose (Keto Hexose) .

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These are Functional Isomers

their structure differs only at

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functional groups.
Stereoisomer's Of Glucose

Stereoisomers are type of

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isomers which have same

molecular formula; structure

differs only in the orientation

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of groups in space.

Glucose Stereoisomers

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D and L Glucose
Anomers
Epimers
D and L Glucose

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Enantiomers/ Mirror images

of each other/Left and Right

Hand

--- Content provided by​ FirstRanker.com ---


Non superimposible/Non

overlapping.

--- Content provided by⁠ FirstRanker.com ---


To identify D and L Glucose

Look at the penultimate carbon atom

--- Content provided by‌ FirstRanker.com ---

of Glucose (C5) /Farthest assymetric

carbon atom from carbonyl carbon

atom.

--- Content provided by FirstRanker.com ---


In D Glucose -OH is at R.H.S.
In L Glucose -OH is at L.H.S.

Enantiomers

--- Content provided by‌ FirstRanker.com ---

Physical and chemical

properties of Enantiomers are

same, except optical rotation.

--- Content provided by​ FirstRanker.com ---


Sugars present in human

body are of `D' series.

--- Content provided by‌ FirstRanker.com ---

Enzyme Racemase

interconvert `D'

and `L' isomers.

--- Content provided by FirstRanker.com ---

Anomers

-Glucose and -Glucose.
Anomers has group variations

--- Content provided by‍ FirstRanker.com ---

at C1 Anomeric carbon atom of

Glucose .

In Fischer's projection at C1

--- Content provided by‌ FirstRanker.com ---

-Glucose has -OH group at

R.H.S

In Fischer's projection at C1

--- Content provided by​ FirstRanker.com ---

-Glucose has ?OH group at

L.H.S.
Glucose anomers of

--- Content provided by‌ FirstRanker.com ---

Haworth's projection shows

as follows.

-Glucose has -OH group

--- Content provided by‍ FirstRanker.com ---


below the plane.

-Glucose has OH group

--- Content provided by⁠ FirstRanker.com ---

above the plane.
In the body

physiologically the most

--- Content provided by‍ FirstRanker.com ---

thermodynamically

stable form of Glucose

is D Glucopyranose .

--- Content provided by‍ FirstRanker.com ---

Anomerism

Glucose anomers are not stable

and tend to interconvert

--- Content provided by‍ FirstRanker.com ---


constantly by opening and

reclosure of ring.

--- Content provided by⁠ FirstRanker.com ---

Anomerism is interconversion of

one form of anomer to another.

Anomerism exhibit Mutarotaion.

--- Content provided by‍ FirstRanker.com ---


Epimers

Epimers are stereoisomers which

--- Content provided by‌ FirstRanker.com ---

has variation in the orientation of

groups at C 2/ C3 / C4 of Glucose.

Epimers of Glucose

--- Content provided by​ FirstRanker.com ---


Galactose (C4 Epimer)
Mannose (C2 Epimer)
Mannose

--- Content provided by⁠ FirstRanker.com ---

Galactose

(C2 Epimer)


--- Content provided by‌ FirstRanker.com ---


(C4 Epimer)

When Mannose and Galactose

--- Content provided by FirstRanker.com ---

structures are compared there

is variation at two different

carbon atoms (C2 and C4).

--- Content provided by FirstRanker.com ---


Mannose and Galactose are

not Epimers but they are

--- Content provided by‌ FirstRanker.com ---

"Diastereoisomers".
Mutarotation

Mutarotation is

--- Content provided by‌ FirstRanker.com ---

change in specific

rotation of an optically

active substance.

--- Content provided by FirstRanker.com ---


Criteria for an optically

active substance to exhibit

--- Content provided by​ FirstRanker.com ---

Mutarotaion:

Anomerism:
In aqueous solution the optically

--- Content provided by FirstRanker.com ---

active substance should exist in two

or more stereoisomeric forms by

ready interconversions.

--- Content provided by⁠ FirstRanker.com ---

Glucose Exhibits Mutarotation

Glucose in aqueous solution shows

`Anomerism'.

--- Content provided by​ FirstRanker.com ---


Glucose in aqueous solution

readily interconvert from

--- Content provided by FirstRanker.com ---

Glucose to Glucose and attain

an equilibrium mixture to exhibit

mutarotation.

--- Content provided by‍ FirstRanker.com ---

Mutarotation is a physico chemical

property

Shown by certain optically active

--- Content provided by‌ FirstRanker.com ---


substances ,who in aqueous solution has

capacity to interconvert from one

--- Content provided by‍ FirstRanker.com ---

stereoisomeric form to other and attain

a constant equilibrium mixture,

This changes an initial specific rotation

--- Content provided by‌ FirstRanker.com ---


to a constant specific rotation with the

passage of time.

--- Content provided by‍ FirstRanker.com ---

Chemical Properties

OR

Chemical Reactions

--- Content provided by​ FirstRanker.com ---


OR

Derivatives Of Glucose
Reduction Reaction Of Sugars

--- Content provided by‍ FirstRanker.com ---


Reduction Reaction

Glucose Sorbitol

--- Content provided by FirstRanker.com ---

(Sugar) (Sugar Alcohol)
During reduction reaction

the C1 carbonyl group

--- Content provided by FirstRanker.com ---

(-CHO) is reduced to primary

alcohol group (-CH2OH).

Sorbitol is

--- Content provided by‍ FirstRanker.com ---


Polyol/Polyhydroxy Alcohol.
Sugar Alcohols taken in food are

of less calorific value.

--- Content provided by‌ FirstRanker.com ---


They yield half energy in

comparison to sugars.

--- Content provided by FirstRanker.com ---

They are poorly absorbed.
Sugar alcohols if ingested reduces

weight.

--- Content provided by⁠ FirstRanker.com ---

They are prescribed for Diabetics.

Abnormal levels of Sorbitol found

in Diabetics, leads to Cataract

--- Content provided by‍ FirstRanker.com ---


Excess Blood Glucose in

Diabetics, get reduced to

--- Content provided by⁠ FirstRanker.com ---

Sorbitol which further

deposits in the lens of eye

and forms Cataract.

--- Content provided by‌ FirstRanker.com ---

Oxidation Reaction Of Sugar

Gluconic Acid

(Aldonic Acid)

--- Content provided by‍ FirstRanker.com ---




(-CHO to -COOH)

--- Content provided by​ FirstRanker.com ---

Oxidation Reaction

Glucose


--- Content provided by⁠ FirstRanker.com ---


Glucuronic Acid

(Uronic Acid)
(-CH2OH to -COOH)

--- Content provided by FirstRanker.com ---

Glucosaccharic Acid

(Dibasic Aldaric Acid)


--- Content provided by⁠ FirstRanker.com ---


(-CHO &-CH2OH to ?COOH)
Aldehyde oxid'n
aldonic

--- Content provided by‍ FirstRanker.com ---

acid

Terminal CH2OH

oxid'n Uronic acid

--- Content provided by‍ FirstRanker.com ---


Aldehyde + Terminal CH2OH oxid'n
Aldaric acid/Saccharic acid
Uronic Acid of Glucose

--- Content provided by⁠ FirstRanker.com ---

Glucoronic acid is component

of Mucopolysaccharides.

Glucuronic acid serve as

--- Content provided by‍ FirstRanker.com ---


conjugating agent in

detoxification reactions.
N-Glucosamine

--- Content provided by‍ FirstRanker.com ---

N Acetyl Glucosamine
N Acetyl Galactosamine
Are Important constituents

of Mucopolysaccharides,

--- Content provided by​ FirstRanker.com ---


Glycoproteins and

Glycolipids

--- Content provided by‌ FirstRanker.com ---



Glucose


--- Content provided by‌ FirstRanker.com ---




Esteification

--- Content provided by‌ FirstRanker.com ---

Glucose -6-Phosphate

Phosphorylation Reaction (Robinson Ester)

OR

--- Content provided by​ FirstRanker.com ---


Glucose-1-Phosphtae

(Cori Ester)
Reducing Property Of Glucose

--- Content provided by‍ FirstRanker.com ---


Reducing property of

Glucose is Enolization/

--- Content provided by‌ FirstRanker.com ---

Tautomerization reaction.

Glucose show efficient

reducing property in

--- Content provided by​ FirstRanker.com ---


alkaline medium .
Glucose in alkaline

medium forms Enediol

--- Content provided by⁠ FirstRanker.com ---


Enediol is a strong

reducing agent which

--- Content provided by​ FirstRanker.com ---

reduces cupric ions to

cuprous ions.

Glucose give positive test results

--- Content provided by‍ FirstRanker.com ---


for following reducing tests:

Benedicts Test
Barfoeds Test

--- Content provided by​ FirstRanker.com ---

Fehlings Test
Nylanders Test.
Benedicts Test-Reduction of Cupric

ions in mild alkaline medium.

--- Content provided by​ FirstRanker.com ---


Barfoeds Test-Reduction of Cupric

ions in weak acidic medium.

--- Content provided by‌ FirstRanker.com ---

Fehlings Test- Reduction of Cupric

ions in strong alkaline medium.

Nylanders Test-Reduction of

--- Content provided by FirstRanker.com ---


Bismuth ions in strong alkaline

medium.

--- Content provided by⁠ FirstRanker.com ---

Glycosides

Glycosides are derivatives of sugar.
Glycosides has Aglycone moiety linked to

--- Content provided by​ FirstRanker.com ---

C1 (anomeric carbon atom) of sugar by an

acetal linkage.

Sugar Acetal Linkage

--- Content provided by⁠ FirstRanker.com ---


Aglycone

Moiety
Aglycone moieties-

--- Content provided by​ FirstRanker.com ---

(Non Sugar, Hydroxyl group

containing compounds)

?Methanol

--- Content provided by FirstRanker.com ---

?Sterol
?Phenol
?Glycerol

Types of Glycosides-

--- Content provided by‍ FirstRanker.com ---


Glucoside

(Contains Sugar as Glucose)

--- Content provided by‍ FirstRanker.com ---


Galactoside

(Contains sugar as Galactose)
Occurrence and Uses of

--- Content provided by​ FirstRanker.com ---


Glycosides

Glycosides are naturally

--- Content provided by‍ FirstRanker.com ---

occurring substances

present in plants and

animal bodies; which are

--- Content provided by​ FirstRanker.com ---


extracted and used as

drugs.

--- Content provided by⁠ FirstRanker.com ---

Glycosides

Examples and Therapeutic Use
S.No

--- Content provided by⁠ FirstRanker.com ---

Examples of

Therapeutic Use

Glycosides

--- Content provided by‌ FirstRanker.com ---


1

Cardiac Glycosides

--- Content provided by FirstRanker.com ---

In treatment of cardiac

Digoxin /Digitonin

insufficiency.

--- Content provided by⁠ FirstRanker.com ---


Ovabain

2

--- Content provided by‌ FirstRanker.com ---

Phlorizin

In treatment of Diabetes

(Glucose Transporter Inhibitor) mellitus.

--- Content provided by​ FirstRanker.com ---


3

Streptomycin

--- Content provided by‍ FirstRanker.com ---

Antibiotic used to treat

bacterial infections.

4

--- Content provided by⁠ FirstRanker.com ---


Glucovanillin

Flavoring agent in Ice

--- Content provided by‍ FirstRanker.com ---

creams and Puddings.

Biomedical Importance of Glucose

Glucose is a reduced compound and

--- Content provided by‌ FirstRanker.com ---


has bond energy in its structure.

Glucose in body cells

--- Content provided by⁠ FirstRanker.com ---

Oxidized/Catabolized to liberate

chemical form of energy-ATP.

1 Glucose molecule on complete

--- Content provided by‌ FirstRanker.com ---


oxidation produces 32 ATPs.
Glucose serve as primary source of

energy to all body cells.

--- Content provided by‌ FirstRanker.com ---


Glucose is an universal fuel of fetus.

Brain, Erythrocytes ,lens cells, spinal

--- Content provided by‍ FirstRanker.com ---

cord, peripheral nerves are completely

dependent on Glucose for its energy.

After well fed condition the free and

--- Content provided by‌ FirstRanker.com ---


excess body Glucose is transformed to

Reservoir /Storage forms of Glucose

--- Content provided by​ FirstRanker.com ---

? Starch (In Plants)

? Glycogen (In animals)

Glucose still in excess is transformed to

--- Content provided by‌ FirstRanker.com ---


Fat (TAG) and stored as depot fat.

(Unlimited).
Glucose is used for biosynthesis

--- Content provided by FirstRanker.com ---


of:

Glucuronic acid
Glucosamine

--- Content provided by FirstRanker.com ---

N-Acetyl Glucosamine
Galactose
Non essential amino acids
Glycosides

--- Content provided by⁠ FirstRanker.com ---

Galactose

Chemistry of Galactose

Galactose is a Monosaccharide

--- Content provided by‍ FirstRanker.com ---

Aldo Hexose
C4 Epimer of Glucose
Occurrence/Sources

Galactose is never found free

--- Content provided by‍ FirstRanker.com ---


It is a component of :

Milk Sugar Lactose
Mucopolysaccharides

--- Content provided by‌ FirstRanker.com ---

Glycolipids and Glycoproteins.
Galactose on reduction form

Dulcitol.

--- Content provided by​ FirstRanker.com ---

Galactose on strong oxidation

forms Mucic acid.

(Galactosaccharic acid).

--- Content provided by⁠ FirstRanker.com ---


Galactose is abnormally elevated

in blood and excreted in urine ?

--- Content provided by FirstRanker.com ---

GALACTOSEMIA.

Biomedical Importance

Galactose has dietary and

--- Content provided by‍ FirstRanker.com ---


calorific value.



--- Content provided by FirstRanker.com ---

Galactose is transformed to

Glucose in Liver and metabolized.
Galactose is used in biosynthesis of

--- Content provided by​ FirstRanker.com ---

Mucopolysaccharides,
Glycoproteins, Glycolipids.

Galactose along with Glucose forms

--- Content provided by FirstRanker.com ---

Lactose in lactating mothers .

Galactose is part of

nerve and brain

--- Content provided by​ FirstRanker.com ---


biochemicals, so

milk is essential to

--- Content provided by‌ FirstRanker.com ---

infants.
Mannose

Mannose is a Monosaccharide
Chemically -Aldo Hexose

--- Content provided by‍ FirstRanker.com ---

C2 Epimer of Glucose

Occurrence/Sources of Mannose

In Plants

--- Content provided by⁠ FirstRanker.com ---

Mannan (Polymer of Mannose)
Biomedical Importance Of

Mannose

--- Content provided by⁠ FirstRanker.com ---

Mannose component of
Glycoproteins.

Mannitol reduced compound of
Mannose is used as Diuretic to

--- Content provided by​ FirstRanker.com ---

treat Acute Renal failure.

Fructose

Fructose is a

--- Content provided by​ FirstRanker.com ---


Monosaccharide

Fructose is Sweetest Sugar.
Laevulose (Laevorotatory)

--- Content provided by FirstRanker.com ---

Chemistry Of Fructose

Fructose is a Keto Hexose
C6H12O6

--- Content provided by‍ FirstRanker.com ---

C2 is anomeric carbon of

Fructose
Occurrence/Sources Of Fructose

--- Content provided by‍ FirstRanker.com ---

In Fruits, Honey
Body cells, Semen.
Fructose is component

of Sucrose.

--- Content provided by​ FirstRanker.com ---

Fructose is more stable in
D Fructofuranose form.
Selivanoff `s test is characteristic

test for Fructose.

--- Content provided by​ FirstRanker.com ---


(positive result-Cherry red

color).

--- Content provided by⁠ FirstRanker.com ---

Fructose on reduction forms

Sorbitol and Mannitol.

Fructose-6-PO4 (Neubergs Ester )

--- Content provided by⁠ FirstRanker.com ---

Fructose-1,6- Bis Phosphate

(Harden Young Ester).

Abnormal excretion of Fructose in

--- Content provided by FirstRanker.com ---


urine is noted in persons suffering

from Essential Fructosuria.
Biomedical Importance Of

--- Content provided by⁠ FirstRanker.com ---


Fructose

Fructose has dietary and calorific

--- Content provided by​ FirstRanker.com ---

value.

In Liver Fructose is transformed to

Glucose and metabolized.

--- Content provided by⁠ FirstRanker.com ---


Fructose present in semen serves as

nutrient for Sperms.
Biomedically Important

--- Content provided by​ FirstRanker.com ---


Disaccharides
Disaccharides are class of

Carbohydrates, chemically

--- Content provided by​ FirstRanker.com ---


composed of two, same or

different Monosaccharide

--- Content provided by‌ FirstRanker.com ---

units, linked by glycosidic

bond.

General Formula of

--- Content provided by​ FirstRanker.com ---


Disaccharides

Cn(H2O)n-1
Glycosidic Bonds

--- Content provided by​ FirstRanker.com ---


Glycosidic bonds are

Acetal/ Ketal bonds

--- Content provided by‍ FirstRanker.com ---

involving the anomeric

carbon of Monosaccharides.

The Aldehyde/Ketone groups

--- Content provided by​ FirstRanker.com ---


participate in glycosidic bond,

Its involvement looses reducing

--- Content provided by⁠ FirstRanker.com ---

property since, they wont remain

free.
Glycosidic bonds are formed

--- Content provided by FirstRanker.com ---

with the interaction of two

hydroxyl groups of adjacent

sugar

--- Content provided by‍ FirstRanker.com ---


residues(Monosaccharide)

with an elimination of water

--- Content provided by FirstRanker.com ---

molecule.
Glycosidic

Linkage

--- Content provided by​ FirstRanker.com ---

Glycosidic bonds are

covalent, strong bonds

,linking one Monosaccharide

--- Content provided by‌ FirstRanker.com ---


to another.
Glycosidic bonds are formed

with / configuration.

--- Content provided by​ FirstRanker.com ---


Different glycosidic bonds

form a different molecule

--- Content provided by⁠ FirstRanker.com ---

with different properties.

Types of Glycosidic bonds:

? ( 1-4)

--- Content provided by⁠ FirstRanker.com ---

? (1-6)
? 1-2
? (1-1)
? (1-4)
Reducing end- End with free

--- Content provided by‌ FirstRanker.com ---


anomeric carbon, not involved

in formation of glycosidic bond.

--- Content provided by FirstRanker.com ---

Non reducing end- End with

no free anomeric carbon, since

involved in formation of

--- Content provided by⁠ FirstRanker.com ---


Glycosidic bond.

Types of Disaccharides
Reducing Disaccharides

--- Content provided by FirstRanker.com ---


Lactose (Milk Sugar)
Maltose
(Malt Sugar, Product of Starch digestion))

--- Content provided by FirstRanker.com ---

Isomaltose (product of Starch digestion)
Lactulose (Laxative)
Cellobiose ( Product of Cellulose)

Non Reducing Disaccharides

--- Content provided by‍ FirstRanker.com ---


Sucrose (Cane Sugar )
Trehalose
Lactose

--- Content provided by FirstRanker.com ---

(Milk Sugar)

Components and Linkage

of Lactose

--- Content provided by FirstRanker.com ---


Lactose is a Reducing Disaccharide



--- Content provided by‌ FirstRanker.com ---

(1-4 ) glycosidic bond

D Galactose

D Glucose

--- Content provided by⁠ FirstRanker.com ---




Source / Occurrence Of Lactose

--- Content provided by FirstRanker.com ---

Milk and Milk products.
Lactating Mothers body.

Biomedical Importance Of Lactose

--- Content provided by⁠ FirstRanker.com ---

Lactose has dietary and calorific

value.

GIT enzyme Lactase digests

--- Content provided by​ FirstRanker.com ---


Lactose by cleaving (1-4)

glycosidic bond and releases free

--- Content provided by‍ FirstRanker.com ---

Galactose and Glucose.
Lactase deficiency in GIT leads

to suffer from Lactose

--- Content provided by‌ FirstRanker.com ---

Intolerance.

Certain bacteria can ferment

lactose to lactic acid - souring of

--- Content provided by​ FirstRanker.com ---


milk

(Lactobacillus).
Lactose may occur in urine during

--- Content provided by​ FirstRanker.com ---


trimester of pregnancy.

Maltose

--- Content provided by‌ FirstRanker.com ---

Maltose is a reducing

Disaccharide

Malt Sugar

--- Content provided by‍ FirstRanker.com ---

Components and Linkage Of

Maltose

D Glucose - D Glucose

--- Content provided by FirstRanker.com ---

(1-4 ) glycosidic bond

Glucose to form Maltose

+

--- Content provided by FirstRanker.com ---


Condensation

Hydrolysis

--- Content provided by‌ FirstRanker.com ---

H2O


Source / Occurrence Of Maltose

--- Content provided by‍ FirstRanker.com ---

Malt grain, Germinating seeds,

Maltova.

In human GIT, source of Maltose

--- Content provided by‌ FirstRanker.com ---


is through Starch and Glycogen

digestion by Amylase

--- Content provided by​ FirstRanker.com ---

activity.
Maltose is obtained in

GIT as an end product of

--- Content provided by‌ FirstRanker.com ---

Starch and Glycogen

digestion.

Biomedical Importance Of

--- Content provided by⁠ FirstRanker.com ---


Maltose

Maltose has dietary and

--- Content provided by⁠ FirstRanker.com ---

calorific value.

GIT enzyme Maltase digests

Maltose by cleaving (1-4)

--- Content provided by‍ FirstRanker.com ---


glycosidic bond and releases

two Glucose units.
Isomaltose

--- Content provided by​ FirstRanker.com ---


Isomaltose is a reducing

Disaccharide.

--- Content provided by‍ FirstRanker.com ---

Isomaltose is a product

of Starch and Glycogen

digestion.

--- Content provided by‍ FirstRanker.com ---


Components and Linkage Of

Isomaltose

--- Content provided by​ FirstRanker.com ---

D Glucose - D Glucose
(1-6 ) glycosidic bond
Source /Occurrence Of Isomaltose

In human GIT

--- Content provided by⁠ FirstRanker.com ---


Isomaltose is obtained

from Starch and

--- Content provided by‌ FirstRanker.com ---

Glycogen digestion

by -Amylase activity.
Biomedical Importance of

--- Content provided by‌ FirstRanker.com ---

Isomaltose

Isomaltose has dietary and

calorific value.

--- Content provided by⁠ FirstRanker.com ---


Isomaltose is digested by GIT

enzyme Isomaltase to release

--- Content provided by​ FirstRanker.com ---

two Glucose units by cleaving

(1-6 ) glycosidic bond.

Cellobiose

--- Content provided by‌ FirstRanker.com ---


Cellobiose is a reducing

Disaccharide.

--- Content provided by​ FirstRanker.com ---

Disaccharide obtained from

Cellulose Digestion.
Components and Linkage Of

--- Content provided by FirstRanker.com ---

Cellobiose

D Glucose - D Glucose
(1-4 ) glycosidic bond
Source / Occurrence of Cellobiose

--- Content provided by​ FirstRanker.com ---


Cellobiose obtained from

Cellulose digestion In GIT of

--- Content provided by⁠ FirstRanker.com ---

ruminants ( Cattle) .

Cellobiose is absent in

human GIT, since enzyme

--- Content provided by FirstRanker.com ---


Cellulase is absent which do

not digest Cellulose.

--- Content provided by‌ FirstRanker.com ---

Biomedical Importance of

Cellobiose

Cellobiose is absent in

--- Content provided by‍ FirstRanker.com ---


human beings.

Not of biomedical

--- Content provided by‍ FirstRanker.com ---

Importance.
Lactulose

Lactulose is a

--- Content provided by​ FirstRanker.com ---

Reducing

Disaccharide

Type, Components and

--- Content provided by​ FirstRanker.com ---


Linkage of Lactulose

D Galactose - D Fructose
(1-4 ) glycosidic bond

--- Content provided by⁠ FirstRanker.com ---

Source / Occurrence Of Lactulose

In Plants
Lactulose:
Prepared by

--- Content provided by⁠ FirstRanker.com ---


alkaline

rearrangement of

--- Content provided by​ FirstRanker.com ---

lactose

Biomedical Importance Of

Lactulose

--- Content provided by⁠ FirstRanker.com ---


Lactulose has therapeutic

value; act as osmolar

--- Content provided by​ FirstRanker.com ---

laxative.

Relieves Chronic

Constipation.

--- Content provided by‌ FirstRanker.com ---

Oral administration of

Lactulose relieves hyper

Ammonaemia in patients of

--- Content provided by​ FirstRanker.com ---


Hepatic Encephalopathy.

Treatment of Systemic

--- Content provided by FirstRanker.com ---

Encephalopathy By Lactulose.

Mechanism of action:
Lactulose is not digestible.
Bacterial flora convert it to Lactic and

--- Content provided by‍ FirstRanker.com ---


Acetic acids that irritate the intestinal

wall.

--- Content provided by⁠ FirstRanker.com ---

Increases acidity of intestine ,this

moves ammonia from blood to the

intestine for neutralization.

--- Content provided by‍ FirstRanker.com ---


Relieves Hyperammonaemia.
Sucrose

Sucrose is a Non Reducing Disaccharide

--- Content provided by‌ FirstRanker.com ---

Cane sugar/ Common Table Sugar /Beet sugar

Components and Linkage Of

Sucrose

--- Content provided by‌ FirstRanker.com ---


D Glucose- D Fructose
1- 2 glycosidic bond.

Why Sucrose is Non reducing?

--- Content provided by⁠ FirstRanker.com ---


Glucose (C1) and Fructose(C2)

anomeric carbon atoms are

--- Content provided by​ FirstRanker.com ---

involved in formation of

Glycosidic bond.

No free anomeric carbon

--- Content provided by‌ FirstRanker.com ---


atoms.

Hence Non reducing.

--- Content provided by​ FirstRanker.com ---

Sucrose has no

free aldehyde

/ketone group

--- Content provided by‍ FirstRanker.com ---


hence non

reducing
Sucrose is

--- Content provided by‌ FirstRanker.com ---


dextrorotatory (d/+)

with specific rotation

--- Content provided by​ FirstRanker.com ---

+66.50.

Source /Occurrence Of Sucrose

In Plants ? Sugar cane,

--- Content provided by⁠ FirstRanker.com ---


Beet root.

Commercially prepared

--- Content provided by‌ FirstRanker.com ---

from sugar cane.
Invert Sugar

Invert sugar is a product

--- Content provided by‌ FirstRanker.com ---

of Sucrose hydrolysis.

Invert Sugar is a product

of Inversion Process.

--- Content provided by‍ FirstRanker.com ---

Invert Sugar is a

hydrolytic mixture of

free Glucose and

--- Content provided by​ FirstRanker.com ---


Fructose obtained from

Sucrose hydrolysis.

--- Content provided by‌ FirstRanker.com ---

Inversion process

A non reducing and

dextrorotatory Sucrose, on acid

--- Content provided by FirstRanker.com ---


hydrolysis/by action of enzyme

Invertase ,

--- Content provided by​ FirstRanker.com ---

Produces a hydrolytic mixture of

free, Glucose(+52.50) and

Fructose(-930) which is reducing

--- Content provided by⁠ FirstRanker.com ---


and laevorotatory(- 20.40).
Invert Sugar is Reducing.

Invert sugar is laevorotatory.

--- Content provided by‍ FirstRanker.com ---


(since Fructose has high magnitude of optical

rotation -930)

--- Content provided by​ FirstRanker.com ---

Invert Sugar is sweeter than Sucrose

(since it contains free Fructose,a sweetest

sugar)

--- Content provided by⁠ FirstRanker.com ---


Uses Of Invert Sugar

Sweetening agent as it

--- Content provided by FirstRanker.com ---

is more sweet than

Sucrose.

Used in adulteration of

--- Content provided by FirstRanker.com ---


Honey.
Biomedical Importance Of Sucrose

Sucrose has dietary and calorific

--- Content provided by‌ FirstRanker.com ---


value.

Sucrase or Invertase enzyme of

--- Content provided by⁠ FirstRanker.com ---

GIT cleaves 1- 2 Glycosidic

bond of Sucrose and release free

Glucose and Fructose ? i.e. Invert

--- Content provided by⁠ FirstRanker.com ---


Sugar.

Biomedically Important

--- Content provided by​ FirstRanker.com ---

Oligosaccharides
Oligosaccharides are

composed of 3-10

--- Content provided by FirstRanker.com ---

Monosaccharide units linked

by glycosidic bonds.

Oligosaccharide may be

--- Content provided by‍ FirstRanker.com ---


branched or unbranched

chain.

--- Content provided by⁠ FirstRanker.com ---

Dietary Oligosaccharides are

not digested by human hence

has no calorific value.

--- Content provided by‍ FirstRanker.com ---


Oligosaccharides are

components of Glycoproteins.
Biomedically Important

--- Content provided by​ FirstRanker.com ---


Polysaccharides/Glycans

Polysaccharides are complex

--- Content provided by‍ FirstRanker.com ---

class of Carbohydrates,

Chemically composed of more

than ten, same or different

--- Content provided by⁠ FirstRanker.com ---


Monosaccharide units or their

derivatives

--- Content provided by FirstRanker.com ---

Repeatedly linked by glycosidic

linkages.
General

--- Content provided by​ FirstRanker.com ---

Formula of

Polysaccharides

(C6H10O5 )n

--- Content provided by​ FirstRanker.com ---


Iodine test is a

characteristic

--- Content provided by‌ FirstRanker.com ---

test for

Polysaccharides
Iodine test is based on Physical

--- Content provided by‌ FirstRanker.com ---

property of adsorption.

Iodine get adsorbed on complex

structure of Polysaccharides to

--- Content provided by‍ FirstRanker.com ---


give characteristic color.

Homopolysaccharides

--- Content provided by⁠ FirstRanker.com ---

Homopolysaccharides are type

of Polysaccharides composed of

more than 10, same type of

--- Content provided by‍ FirstRanker.com ---


Monosaccharide units

repeatedly linked by glycosidic

--- Content provided by‌ FirstRanker.com ---

bonds.
Glucosan

Type of Homopolysaccharide
Repeating unit,of Glucosan is

--- Content provided by‍ FirstRanker.com ---


Glucose

Glucosan is a Polymer of

--- Content provided by​ FirstRanker.com ---

Glucose.

Examples Of Glucosans

Starch

--- Content provided by​ FirstRanker.com ---

Glycogen
Cellulose
Dextrin
Dextran
Fructosan

--- Content provided by FirstRanker.com ---


Type of

Homopolysaccharide

--- Content provided by FirstRanker.com ---

Repeating unit in

Fructosan is Fructose.

Fructosan is a Polymer of

--- Content provided by‌ FirstRanker.com ---


Fructose.

Example Of Fructosan

--- Content provided by​ FirstRanker.com ---

Inulin
Starch



--- Content provided by FirstRanker.com ---

Plant Homopolysaccharide

Chemistry Of Starch

Starch is Glucosan

--- Content provided by​ FirstRanker.com ---

Repeating Unit -
D Glucose (approx 7000)
Components of Starch-
Amylose and Amylopectin
Amylose

--- Content provided by‌ FirstRanker.com ---


Amylose is 15-20%
Linear structure
D Glucose linked by

--- Content provided by⁠ FirstRanker.com ---

(1-4) glycosidic bond.


Amylopectin

--- Content provided by⁠ FirstRanker.com ---



Amylopectin is 80-85 %
Branched structure
Branching point appears after every

--- Content provided by⁠ FirstRanker.com ---

25-30 Glucose units.
It has (1-6) glycosidic bond at branching

point.

--- Content provided by‌ FirstRanker.com ---

(1-4) glycosidic bonds in linear structure.

Starch is white ,odourless, tasteless

powder .

--- Content provided by FirstRanker.com ---


Starch is insoluble in cold water, but get

solubilized on heating and form gel

--- Content provided by FirstRanker.com ---

/paste.

Starch is a non reducing Carbohydrate.
Starch -Negative Benedicts Test.
Starch on Iodine reaction gives blue

--- Content provided by⁠ FirstRanker.com ---


color.
Source/Occurrence Of Starch

Plants- Seeds, Tubers, Roots, Raw fruits.

--- Content provided by FirstRanker.com ---


Dietary Sources of Starch.

Grains- Rice ,Wheat, Jawar, Bajra
Potatoes

--- Content provided by​ FirstRanker.com ---

Beetroot.
Sago (Tapioca)
Vermicelli
Suji.
Raw Mangoes

--- Content provided by‌ FirstRanker.com ---


Biomedical Importance Of Starch

Starch is a storage form of Glucose

--- Content provided by⁠ FirstRanker.com ---

and serves as reservoir of energy in

plants.

To humans Starch is a predominant

--- Content provided by⁠ FirstRanker.com ---


form of dietary Carbohydrate

ingested through foods which has

--- Content provided by‌ FirstRanker.com ---

high dietary and calorific value.
Digestion of Starch

In mouth- by salivary Amylase
In intestine -by pancreatic Amylase

--- Content provided by‌ FirstRanker.com ---


Amylase cleaves, (1-4) glycosidic

bonds of Amylose and Amylopectin and

--- Content provided by⁠ FirstRanker.com ---

releases Maltose and Isomaltose.

Maltose and Isomaltose is then

digested by Maltase and

--- Content provided by FirstRanker.com ---


Isomaltase to release free Glucose

units.

--- Content provided by‍ FirstRanker.com ---

Thus Starch on digestion gives

thousands of free Glucose units

which have high calorific value.

--- Content provided by‍ FirstRanker.com ---

Glycogen



Animal Homopolysaccharide

--- Content provided by‌ FirstRanker.com ---

Animal Starch

Chemistry Of Glycogen

Glycogen is chemically

--- Content provided by‌ FirstRanker.com ---


Glucosan.

Repeating Unit ?

--- Content provided by‍ FirstRanker.com ---

D Glucose (25-30 thousand

Glucose units)
Glycogen is like Amylopectin

--- Content provided by‌ FirstRanker.com ---

structure ,but it is highly branched

Branching points appear after

every 8-10 Glucose units, linked by

--- Content provided by FirstRanker.com ---


(1-6) glycosidic bond.

Linear structure has Glucose linked

--- Content provided by FirstRanker.com ---

with (1-4) glycosidic bond.



Glycogen

--- Content provided by​ FirstRanker.com ---

Glycogen has more a(16)

branches.

The highly branched structure

--- Content provided by​ FirstRanker.com ---


permits rapid glucose release

from glycogen stores, in muscle

--- Content provided by‍ FirstRanker.com ---

during exercise.

Source/Occurrence Of Glycogen

Glycogen is present in

--- Content provided by‍ FirstRanker.com ---


animal-

Liver (75 gm)

--- Content provided by FirstRanker.com ---

Muscle (125 gm).
Non-Veg diet is a source

of dietary Glycogen to

--- Content provided by‍ FirstRanker.com ---

human beings, which has

high dietary and calorific

value.

--- Content provided by​ FirstRanker.com ---


After well fed condition, free

and excess of Glucose is

--- Content provided by‌ FirstRanker.com ---

condensed to Glycogen via

Glycogenesis.

Rate of Glycogen synthesis ?

--- Content provided by‌ FirstRanker.com ---


Liver- 6-8 %,
Muscle 1-2 %
Glycogen is broken

--- Content provided by‍ FirstRanker.com ---

down to Glucose via

Glycogenolysis when

body Glucose lowers in

--- Content provided by⁠ FirstRanker.com ---


fasting and starvation

condition.

--- Content provided by​ FirstRanker.com ---

Biomedical Importance Of

Glycogen

Glycogen is a storage form of

--- Content provided by‍ FirstRanker.com ---


Glucose in animal and human body

after well fed conditions.

--- Content provided by FirstRanker.com ---

It serves as reservoir of Glucose

which can be used in emergency

conditions

--- Content provided by‌ FirstRanker.com ---


( Fasting /Between meals )


Glucose stored in

--- Content provided by​ FirstRanker.com ---


polymeric/condensed

form minimizes osmotic

--- Content provided by‍ FirstRanker.com ---

effects and occupy less

space.

Glycogen produces less

--- Content provided by⁠ FirstRanker.com ---


osmotic pressure and occupy

small space.

--- Content provided by⁠ FirstRanker.com ---

Glycogen is sparingly soluble in

water.

On Iodine reaction Glycogen

--- Content provided by‍ FirstRanker.com ---


gives deep red color.
Dietary Glycogen in

GIT is digested by

--- Content provided by⁠ FirstRanker.com ---


- Amylase to Maltose

and Isomaltose and

--- Content provided by⁠ FirstRanker.com ---

finally to thousands of

Glucose Units.

Liver Glycogenolysis

--- Content provided by​ FirstRanker.com ---


in Human body

regulates Blood

--- Content provided by‌ FirstRanker.com ---

Glucose levels in

fasting condition.
Muscle

--- Content provided by⁠ FirstRanker.com ---

Glycogenolysis

provides energy for

muscle activities in

--- Content provided by‌ FirstRanker.com ---


fasting condition.



--- Content provided by‍ FirstRanker.com ---

Cellulose

Non digestible carbohydrate

Cellulose serve as dietary

--- Content provided by⁠ FirstRanker.com ---


Fiber.
Chemistry Of Cellulose

Cellulose is a Glucosan

--- Content provided by‌ FirstRanker.com ---

Repeating Unit - D Glucose.
(approx 2,500- 14,000 ).

Cellulose is a linear ,unbranched

--- Content provided by​ FirstRanker.com ---

structure where D Glucose units

repeatedly linked by (1-4 ) glycosidic

bonds.

--- Content provided by‌ FirstRanker.com ---


Source/Occurrence Of Cellulose

Cellulose is an abundant carbohydrate of

--- Content provided by‍ FirstRanker.com ---

nature exclusively present in Plants cell wall.

Dietary rich sources of Cellulose
Whole Grains (outer covering)
Green leafy vegetables

--- Content provided by‌ FirstRanker.com ---

Cabbage, Cucumber
Legumes, Nuts, Beans
Dates
Fruits and Vegetable salads.
Biomedical Importance of

--- Content provided by​ FirstRanker.com ---


Cellulose

In plants Cellulose

--- Content provided by⁠ FirstRanker.com ---

present in cell wall

provides structural

and mechanical

--- Content provided by‍ FirstRanker.com ---


support.



--- Content provided by‍ FirstRanker.com ---

Wood, cotton and

paper are composed

primarily of cellulose.

--- Content provided by​ FirstRanker.com ---

In humans, dietary

Cellulose is not digested

and absorbed.

--- Content provided by​ FirstRanker.com ---


Enzyme Cellulase is absent

in human GIT.

--- Content provided by⁠ FirstRanker.com ---

Cellulose has no calorific

value.

Dietary

--- Content provided by‍ FirstRanker.com ---


Cellulose in

humans serves

--- Content provided by⁠ FirstRanker.com ---

as dietary fiber.
Cellulose has Effect on Fecal Mass

Formation

--- Content provided by FirstRanker.com ---

Cellulose acts as a roughage.
It holds water ,helps in forming soft and bulky

feces.

--- Content provided by‍ FirstRanker.com ---

Increases intra luminal pressure.
Reduces transit time of feces to remain in gut.
Eliminates daily, metabolic wastes and toxins

out of the body, through feces.

--- Content provided by​ FirstRanker.com ---


Defecation with greater ease and good

frequency.

--- Content provided by‌ FirstRanker.com ---

Cellulose prevents

constipation, and reduces risk

of :

--- Content provided by⁠ FirstRanker.com ---


vcolon cancer
vvaricose veins
v diverticulosis of intestine
v hemorrhoids

--- Content provided by‍ FirstRanker.com ---

Cellulose reduces the absorption

of :

Glucose

--- Content provided by⁠ FirstRanker.com ---

Cholesterol

Ameliorate the conditions of :

?Diabetes mellitus

--- Content provided by​ FirstRanker.com ---

? Atherosclerosis respectively.

Cellulose being non

calorific and

--- Content provided by FirstRanker.com ---


possessing high satiety

value helps in

--- Content provided by FirstRanker.com ---

managing obesity in

humans.
For maintenance of good health

--- Content provided by⁠ FirstRanker.com ---

ingest dietary fibers.

RDA for dietary fiber to

maintain good health:

--- Content provided by‌ FirstRanker.com ---


Adults= 20-25 gm/day.
Children's= 5-10 gm/day.

Disadvantage of dietary

--- Content provided by⁠ FirstRanker.com ---


Cellulose-

It decreases absorption of

--- Content provided by FirstRanker.com ---

minerals.
Dextrin

Intermediate

--- Content provided by‌ FirstRanker.com ---

hydrolytic

product of Starch

digestion

--- Content provided by FirstRanker.com ---


Chemistry Of Dextrin

Dextrin is a Glucosan
Repeating Unit - D Glucose.

--- Content provided by⁠ FirstRanker.com ---


Dextrin is less complex than starch

structure.

--- Content provided by‍ FirstRanker.com ---

Dextrin is broken Starch molecule.
Types Of Dextrin

Amylodextrin -Violet to Iodine reaction

--- Content provided by FirstRanker.com ---

Erythrodextrin- Red to Iodine reaction

Achrodextrin -Colorless to Iodine reaction.
Source/Occurrence of Dextrin

--- Content provided by FirstRanker.com ---

In human GIT Dextrin is obtained as

an intermediate hydrolytic product

of Starch digestion by the action of

--- Content provided by‌ FirstRanker.com ---


-Amylase activity.

Dextrin is Present in commercially

--- Content provided by‍ FirstRanker.com ---

prepared infant foods.

Biomedical Importance Of Dextrin

Dextrin has mucilage

--- Content provided by⁠ FirstRanker.com ---


nature(sticky) hence used as

binding and adhesive agent.

--- Content provided by FirstRanker.com ---

Dextrin is used as infant food.
Dextran

Dextran is a Bacterial

--- Content provided by​ FirstRanker.com ---

Homopolysaccharide

Chemistry Of Dextran

Dextran is a Glucosan

--- Content provided by​ FirstRanker.com ---

Repeating Unit ? D Glucose.

Highly branched, complex, network

like structure

--- Content provided by‌ FirstRanker.com ---


Glucose units linked by

(1-4) ,(1-6) and (1-3) glycosidic

--- Content provided by‌ FirstRanker.com ---

linkages.
Source/Occurrence Of Dextran

Dextran is obtained from

--- Content provided by FirstRanker.com ---

Sucrose solution

incubated with bacteria

Leuconostoc

--- Content provided by FirstRanker.com ---


mesenteroides
Biomedical Importance Of Dextran

Dextran solution is high molecular

--- Content provided by‍ FirstRanker.com ---


weight, viscous solution with

osmotic pressure equivalent to

--- Content provided by​ FirstRanker.com ---

plasma Albumin.

Dextran maintains blood volume

and osmotic pressure.

--- Content provided by‌ FirstRanker.com ---


Dextran solution is used as
Plasma substitute/plasma

volume expander

--- Content provided by‌ FirstRanker.com ---


Dextran infusion manages a

hemorrhagic case and

--- Content provided by​ FirstRanker.com ---

prevent from

hypovoluemic shock.
Sephadex

--- Content provided by FirstRanker.com ---

Sephadex is a modified Dextran.
The dextran macromolecules are cross-

linked to give a three-dimensional

--- Content provided by​ FirstRanker.com ---

network of polysaccharide chains.

Insoluble in water but absorb water

and swell.

--- Content provided by⁠ FirstRanker.com ---


Uses: In Chromatographic separation.

Fructosan

--- Content provided by FirstRanker.com ---

Homopolysaccharide

with repeating units as

Fructose.

--- Content provided by‌ FirstRanker.com ---

Inulin

Diagnostic Carbohydrate
Diagnoses Kidney Function-

--- Content provided by‌ FirstRanker.com ---

GFR of Kidney.

Chemistry Of Inulin

Inulin is a Fructosan

--- Content provided by‌ FirstRanker.com ---

Repeating Unit-
D Fructose units (33-35 units)
Repeatedly linked by
(1-2) glycosidic bonds.
Source/Occurrence Of Inulin

--- Content provided by​ FirstRanker.com ---


Naturally present in Plants

Roots and tubers of

--- Content provided by​ FirstRanker.com ---

Dandelions

Onion and Garlic bulbs

Chicory Plant.

--- Content provided by​ FirstRanker.com ---


Biomedical Importance Of Inulin



--- Content provided by​ FirstRanker.com ---

Inulin is non digestible and non

absorbable form in human GIT, hence

have no calorific value.

--- Content provided by⁠ FirstRanker.com ---


Inulin solution infused intravenously

during Inulin Clearance Test,

--- Content provided by FirstRanker.com ---

checks Glomerular Filtration Rate

(GFR) of Kidney (120-125 ml/min).
Heteropolysaccharides

--- Content provided by‍ FirstRanker.com ---

Heteropolysaccharides are type

of Polysaccharides composed of

more than 10 different

--- Content provided by‌ FirstRanker.com ---


Monosaccharide units or

their derivatives repeatedly

--- Content provided by‌ FirstRanker.com ---

linked by glycosidic bonds.

Animal Heteropolysaccharides
Mucopolysaccharides (MPS)

--- Content provided by​ FirstRanker.com ---

Human Heteropolysaccharides
Animal Heteropolysaccharides
Glycosaminoglycans (GAGs)

Mucopolysaccharides

--- Content provided by⁠ FirstRanker.com ---


were first isolated from

Mucin hence the name

--- Content provided by⁠ FirstRanker.com ---

Mucopolysaccharides.
Mucopolysaccharides

chemically composed of more

--- Content provided by⁠ FirstRanker.com ---

than 10 Monosaccharide units

and its derivatives repeatedly

linked by glycosidic bonds.

--- Content provided by FirstRanker.com ---


Mucopolysaccharides

are complex, long,

--- Content provided by FirstRanker.com ---

linear, unbranched,

polyanionic.
The Glycosaminoglycans has

--- Content provided by‌ FirstRanker.com ---

Disaccharide repeating unit

linked by glycosidic bonds of

Uronic Acid

--- Content provided by FirstRanker.com ---


Amino Sugar


Uronic Acid ?

--- Content provided by FirstRanker.com ---

Glucuronic acid /Iduronic acid
(Iduronic acid is 5' Epimer of Glucuronic acid)

Amino Sugar-
Glucosamine / Galactosamine

--- Content provided by⁠ FirstRanker.com ---

(Acetylated or Sulfated or Both)
Jeanloz suggested the

term GAG's as these

--- Content provided by FirstRanker.com ---

biomolecules has

amino sugars as

repeating units.

--- Content provided by‍ FirstRanker.com ---


Properties of GAG's

GAG's are polyanionic and acidic due

--- Content provided by FirstRanker.com ---

to presence of

?COO- and SO4-- -

GAG's are hydrophillic and attract

--- Content provided by FirstRanker.com ---


water and helps in distributing

water.
MPS due to repulsion of

--- Content provided by​ FirstRanker.com ---


charges:

It appear slippery or sticky

--- Content provided by​ FirstRanker.com ---

in appearance/mucus like

secretions

It expand to occupy large

--- Content provided by‍ FirstRanker.com ---


space.

MPS/GAG's imparts following

--- Content provided by FirstRanker.com ---

physical properties-

Turgor

High Viscosity

--- Content provided by‌ FirstRanker.com ---


High Density

High Buoyancy.
Body Mucopolysaccharides

--- Content provided by⁠ FirstRanker.com ---


Acidic Non Sulfated MPS:

Hyaluronic Acid

--- Content provided by‍ FirstRanker.com ---

Acidic Sulfated MPS:

Heparin
Heparan Sulfate
Chondritin Sulfate

--- Content provided by‌ FirstRanker.com ---

Dermatan Sulfate
Keratan Sulfate

Neutral MPS:

--- Content provided by‍ FirstRanker.com ---

Blood Group Substances
Hyaluronic Acid

Acidic Non sulfated MPS

--- Content provided by‌ FirstRanker.com ---

Repeating Disaccharide Unit

Glucuronic acid

NAcetylGlucosamine.

--- Content provided by‍ FirstRanker.com ---


Occurrence and Functions Of
Hyaluronic acid

Hyaluronic acid is present as

--- Content provided by FirstRanker.com ---


ground substance/cementing
substance in extra cellular
spaces of connective tissue.
Hyaluronic acid in

--- Content provided by‍ FirstRanker.com ---


synovial fluid of joints
and vitreous humor of
eye serve as lubricant
and shock absorbant.

--- Content provided by​ FirstRanker.com ---


Hyaluronic acid
around ovum
gives protection.
Hyaluronic acid

--- Content provided by‍ FirstRanker.com ---


plays role in cell
migration during
morphogenesis.

--- Content provided by​ FirstRanker.com ---

Enzyme "Hyaluronidase " hydrolyses

Hyaluronic acid.

Hylauronidase present in head of sperm

--- Content provided by⁠ FirstRanker.com ---


, hydrolyzes the Hyaluronic acid present on

ovum which facilitates its penetration

--- Content provided by​ FirstRanker.com ---

and fertilization.

Snake venom is rich in Hyaluronidase,

thus snake bite hydrolyzes and liquifies the

--- Content provided by⁠ FirstRanker.com ---


Hyaluronic acid present in extracellular

spaces of cells ( TOXIN )
Heparin

--- Content provided by⁠ FirstRanker.com ---


Most Acidic Sulfated MPS.
Disaccharide Repeating Unit:

Glucuronate sulfate ( minor

--- Content provided by​ FirstRanker.com ---


)

Iduronate sulfate (major)
N-Glucosamine Sulfate.

--- Content provided by⁠ FirstRanker.com ---


?Heparin, a soluble

glycosaminoglycan found in granules

--- Content provided by⁠ FirstRanker.com ---

of mast cells and is highly sulfated.
?Heparin has an extended helical

conformation.
Occurrence and Functions Of

--- Content provided by‌ FirstRanker.com ---


Heparin

Heparin present in blood vessels,

--- Content provided by⁠ FirstRanker.com ---

Liver, Lung, Spleen and

Monocytes

Heparin is a natural anticoagulant.

--- Content provided by‍ FirstRanker.com ---

Prevents intravascular blood

coagulation by inactivating

clotting factor IX and XI.

--- Content provided by FirstRanker.com ---

When released into the

blood, it inhibits clot

formation by interacting

--- Content provided by⁠ FirstRanker.com ---


with the protein

antithrombin.

--- Content provided by⁠ FirstRanker.com ---

Heparin is an indirect

Thrombin inhibitor .

Rapid inactivator of Thrombin.

--- Content provided by‍ FirstRanker.com ---

Thus antithrombotic agent.
Prevents intravascular blood

clotting.
Heparin releases

--- Content provided by FirstRanker.com ---


enzyme Lipoprotein

Lipase from endothelial

--- Content provided by‌ FirstRanker.com ---

lining and serve as

coenzyme for it.

Lipoprotein Lipase is

--- Content provided by​ FirstRanker.com ---


Lipid clearing Enzyme

of blood.
Therapeutic Use of Heparin

--- Content provided by⁠ FirstRanker.com ---


Heparin is infused to prevent and

treat thrombous located in

--- Content provided by‍ FirstRanker.com ---

vein/artery in MI cases.

Deep Vein Thrombosis.
Pulmonary Embolism
Strokes

--- Content provided by‌ FirstRanker.com ---


Heparin(LMW Heparin)

injections are given to

--- Content provided by​ FirstRanker.com ---

M.I patients

To liquify blood ,prevent

thrombosis and clear

--- Content provided by‌ FirstRanker.com ---


blood with lipids.
Heparan Sulfate


--- Content provided by​ FirstRanker.com ---


Acidic Sulfated Mucopolysaccharide

Disaccharide repeating units
Glucuronate Sulfate (major)/ Iduronate

--- Content provided by FirstRanker.com ---


Sulfate (minor)-N Acetyl Glucosamine

Occurrence and Functions Of

--- Content provided by⁠ FirstRanker.com ---

Heparan Sulfate

Heparan Sulfate present on

extracellular cell surfaces or

--- Content provided by⁠ FirstRanker.com ---


plasma membranes and serves

as receptors

--- Content provided by‌ FirstRanker.com ---

Participate in cell growth, cell

adhesion, and cell -cell

communication.

--- Content provided by‌ FirstRanker.com ---

Heparan sulfate in basement

membrane of kidney helps in

determining charge selectiveness

--- Content provided by‍ FirstRanker.com ---


of Glomerular filtration.

Heparan sulfate are also

--- Content provided by⁠ FirstRanker.com ---

components of aorta, liver,

fibroblasts, synaptic and vesicles.

Chondritin Sulfate

--- Content provided by⁠ FirstRanker.com ---




Acidic Sulfated

--- Content provided by‌ FirstRanker.com ---

Mucopolysaccharid

e
Disaccharide Repeating Unit
Glucuronate-

--- Content provided by‍ FirstRanker.com ---

N ?Acetyl Galactosamine ?Sulfated.

Chondritin Sulfate A-4 sulfated.

Chondritin sulfate C-6 sulfated.

--- Content provided by FirstRanker.com ---


Occurrence and Functions Of

Chondritin Sulfate

--- Content provided by⁠ FirstRanker.com ---

Chondritin Sulfate is present in

connective tissues-bones,

cartilage, tendons .

--- Content provided by⁠ FirstRanker.com ---


It gives mechanical strength,

compressibility and support to

--- Content provided by​ FirstRanker.com ---

connective tissues.
Chondritin sulfate

present in cornea

--- Content provided by‍ FirstRanker.com ---

give over all shape to

eye.

Dermatan Sulfate

--- Content provided by FirstRanker.com ---


Acidic Sulfated Mucopolysaccharide.
Chondritin sulfate-B / -Heparin

Disaccharide Repeating Unit

--- Content provided by​ FirstRanker.com ---

L-Iduronate-
N-AcetylGalactosamine-sulfated
Occurrence and Functions Of

Dermatan Sulfate

--- Content provided by⁠ FirstRanker.com ---


Dermatan sulfate especially present in

skin, blood vessels and heart valves

--- Content provided by⁠ FirstRanker.com ---

gives mechanical strength and

structural support to these tissues.


--- Content provided by‍ FirstRanker.com ---

Dermatan sulfate plays structural

role in sclera of eye.

Keratan Sulfate

--- Content provided by FirstRanker.com ---


Acidic Sulfated Mucopolysaccharide

Keratan Sulfate is a MPS without Uronic

--- Content provided by‌ FirstRanker.com ---

acid instead contains Galactose.

Disaccharide Repeating Unit
Galactose-N-Acetyl Glucosamine ?sulfated
Keratan sulfate present in cartilage,

--- Content provided by‍ FirstRanker.com ---


aorta walls, gives structural

supports and mechanical strength.

--- Content provided by⁠ FirstRanker.com ---


Keratan sulfate present in cornea

and lens of eye has role in lens

--- Content provided by⁠ FirstRanker.com ---

transparency and shape of eye.

Blood Group Substances

Blood group substances are Neutral MPS

--- Content provided by‍ FirstRanker.com ---


Components of Blood Group

substances

--- Content provided by‍ FirstRanker.com ---

Galactose, Fucose,
N-Acetyl Glucosamine, N-Acetyl

Galactosimne

--- Content provided by​ FirstRanker.com ---


Blood group substances

neutral MPS, present

--- Content provided by​ FirstRanker.com ---

on cell surfaces of

Erythrocytes serves as

blood group antigens.

--- Content provided by‍ FirstRanker.com ---


Applied aspects of

Mucopolysaccharides/ GAGs
On ageing or during

--- Content provided by‌ FirstRanker.com ---


pathogenesis the biosynthesis of

certain specific

--- Content provided by‍ FirstRanker.com ---

Mucopolysaccharide

/Glycosaminoglycan is either

increased or decreased leading

--- Content provided by⁠ FirstRanker.com ---


to disorders and manifestations.

Tumors cells-
Increased Hyaluronic acid

--- Content provided by FirstRanker.com ---


(Increases cell migration)

Decreased Heparan Sulfate

--- Content provided by⁠ FirstRanker.com ---

(Decreases cell adhesion)

This Increases Metastasis
Rheumatic Arthritis-
Rheumatic nodule shows

--- Content provided by​ FirstRanker.com ---


increased Hyaluronic acid

deposition.

--- Content provided by‌ FirstRanker.com ---

Atherosclerotic

plaque-

Excess production of

--- Content provided by‌ FirstRanker.com ---


Dermatan Sulfate

Decreased production

--- Content provided by FirstRanker.com ---

of heparin.
Osteoarthritis-
Imbalance biosynthesis

of Hyaluronic acid,

--- Content provided by FirstRanker.com ---


Chondritin Sulfate and

Keratan Sulfate

--- Content provided by⁠ FirstRanker.com ---

Mucoproteins

Or

Proteoglycans

--- Content provided by⁠ FirstRanker.com ---

Mucoproteins/

Proteoglycans

are conjugated

--- Content provided by​ FirstRanker.com ---


Proteins.

Mucopolysaccharides/
Glycosaminoglycans

--- Content provided by​ FirstRanker.com ---


(Prosthetic group) are never

found free but always covalently

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linked to a core protein by N-

Glycosidic/O-Glycosidic bond to

formMucoproteins/Proteoglycan

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O-Glycosidic /N-Glycosidic bond

Glycosaminoglycans

Core

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Protein

Mucoproteins have

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Carbohydrate

content more than

10 % and are viscous

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in nature.
Mucoproteins/
Proteoglycans are

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more complex,

viscous, highly dense,

molecular aggregates.

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GAG chains

core

protein

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Proteoglycans are

composed of as many as

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200 GAG chains covalently

bonded to a core protein

via Serine/ Threonine side

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chains.
Molecular weight range: 105 ? 107 Daltons.

GAG chains linked:

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Hyaluronic acid

Chondroitin sulfate

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Heparan sulfate

Dermatan sulfate

Keratan sulfate

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Examples of Proteoglycans

Aggrecan

(Hyaluronate-Core Protein non

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covalently linked In Cartilage)

Biglycan

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Beta Glycan

Decorin

Serglycin

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Syndecan

Perlecan

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Versican
Mucoproteins has properties like of

Mucopolysaccharides.

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Mucoproteins widely distributed in

Extracellular Matrix of connective

tissues ( Bone and Cartilage).

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Mucoproteins provide structural

framework and mechanical

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support to those tissues which

constitute them.

Mucopolysaccharidoses

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Mucopolysaccharidoses are group

of inherited disorders related to

defective Mucopolysaccharide

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metabolism.

Cause:
Impaired degradation of GAG's

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by defective Lysosomal

enzymes.

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Half life period of GAG's

is short.

3-10 days for most of the

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GAG's.

120 days for Keratan ?SO4.
Biochemical Alterations :

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No catabolism of GAG's.
Abnormal widespread intra

Lysosomal deposition of GAG's

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in functional tissues affecting

their functions.

Excretion of MPS in Urine.

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Mucopolysaccharidoses

Syndrome

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Enzyme Defect

Accumulated GAG

Type

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(Lysosomal)

I

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Hurler's

- L Iduronidase

DS, HS

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Syndrome

II

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Hunters'

Iduronate Sulphatase

DS , HS

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Syndrome

III

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Sanfilippo's

Heparan Sulphatase

HS

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Syndrome

IV

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Morquio's

Galactosamine

KS, CS

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Syndrome

Sulphatase.

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V

Scheie's

L- Iduronidase

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DS

Syndrome

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VI

Maroteaux

N-

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DS

Lamy

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AcetylGalactosamine

Syndrome

-4-sulphatase.

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VII

SLY's

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- Glucuronidase.

DS, HS

IX

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Naowicz

Hyaluronidase

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HA

Syndrome
All Mucopolysaccharidoses

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are of autosomal recessive

inheritance.

Hunter's Syndrome is of X

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linked inheritance.

Consequences /Clinical Manifestations:

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Lysosomal vesicles become swollen with

incomplete degraded GAG's in it.

Coarse facial features.

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Thick skin, skeletal damage.
Corneal Opacity, Hearing loss.
Mental Retardation.
Hepatosplenomegaly.
Cardio pulmonary defects.

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Growth deficiency and skeletal dysplasia.
Diagnosis:

Measuring concentration of

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Lysosomal Hydrolases.

Detection of GAG in Urine.

Plant Heteropolysaccharides

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Agar-Agar

Agar is obtained from red algae.(Sea weed)
Agar is formed of two main components,

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Agarose and Agaropectin.

Agarose is a neutral galactose polymer, free

from sulfate.

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Agaropectin is formed of galactose and

galacturonic acid units partially esterified

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with sulfuric acid.

Uses of Agar

Preparation of bacteriological

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culture media.

Emulsifier, thickener for ice

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creams,puddings.

Laxative-for treatment of ulcers

and chronic constipation.

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Pectin

Obtained from apple pomace and

inner portion of citrus rind.

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Form viscous solutions in water.
Composed of Arabinose, Galactose

and Galactouronic acid.

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Average molecular weight 100,000-

250,000.

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Uses Of Pectin

Pectin is topically applied as a paste in

cases of burns and ulcers.

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It acts as a detoxifying agent by

conjugation with toxins.

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It is of great importance in treatment of

diarrhea and dysentery.

It is used as a gel and emulsion stabilizer

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and in manufacture of jellies and jams.
Glycoproteins

Glycoproteins are conjugated

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proteins

Where the prosthetic group,

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branched or unbranched chain of

Oligosaccharide

Is linked to a protein backbone with

--- Content provided by‍ FirstRanker.com ---

O-Glycosidic or N-Glycosidic

linkage.


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O-Glycosidic/N-Glycosidic bonds

Oligosaccharide

Chain

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Protein

Carbohydrate

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content of

Glycoprotein is

less than 10%.

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N-linked saccharides are

attached via the amide

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nitrogens of Asparagine

residues .

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O-linked saccharides are

attached to hydroxyl groups

of Serine, Threonine or

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hydroxy Lysine .

N-linked Glycoproteins
O-linked Glycoproteins

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Oligosaccharides have
different sequences of monosaccharide units
different sequences of glycosidic linkages.
different kinds of branching.

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This imparts a very high degree of

diversity for Oligosaccharides and
their structure-function relationships

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Varied glycosidic linkages in

Oligosaccharide chain of

Glycoproteins create enormous

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variability required for

identifying different cells.

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This variation is the basis for

the mechanism of cell-cell

recognition.

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N-acetylneuraminate (N-acetylneuraminic acid,

also called sialic acid) is often found as a terminal

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residue of oligosaccharide chains of glycoproteins.
Sialic acid imparts negative charge to

glycoproteins, because its carboxyl group tends to

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dissociate a proton at physiological pH, as shown

here.
Significance of Oligosaccharide

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chains in Glycoproteins :

Stabilizes the Proteins against

denaturation.

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Protect Proteins from Proteolytic

degradation.

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Enhances the solubility.
Serve as a recognition signals to

facilitate cell-cell interaction.

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Glycoproteins of human body:

All plasma proteins are

Glycoproteins.

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Structural protein Collagen of

bone is glycoprotein.

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Enzymes- Ribonuclease-B,

Alkaline Phosphatase.
Hormonal receptors on cell

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membranes.

Glycophorin is a major integral

membrane Glycoprotein of RBC's.

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Lubricant Mucin.
Proteins-Transferrin and

Ceruloplasmin are Glycoproteins.

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Glycoproteins and Glycolipids are

Glycocalyx of cell membranes.

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Clotting factor-Prothrombin.
Hormones-Thyroglobulin,

Erythropoietin, TSH, HCG.

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Immunoglobulin- IgG, IgA, IgD,

IgE, IgM.
Biomedical Importance's of

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Carbohydrates.

Energy Aspects of

Carbohydrates/Carbohydrates

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have dietary and calorific value

Carbohydrates serve as primary

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source of energy, with calorific

value 4Cal/gm.

Carbohydrates of Dietary and

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Calorific value

Starch (Predominant-Grains, Potatoes, Vegetables)
Glycogen (Non Veg diet)

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Sucrose (Common table Sugar)
Lactose (Milk and Milk Products)
Maltose (Starch digestion, Malt)
Glucose (Glucon-D, Fruits)
Fructose (Fruits, Honey)

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Reserve Store Capacity Of

Carbohydrates:

Dietary Carbohydrate (Glucose)

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when free and excess in the body

get transformed to reserve store

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forms Glycogen and Triacylglycerol

(Fat), which are utilized during

emergency conditions.

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Non digestible Carbohydrate-

Cellulose serves as dietary

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fiber :

Cellulose act as roughage and

prevents from constipation.

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Cellulose has no calorific value

hence help in management of

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obesity.
Carbohydrates as Dietary Fiber

Cellulose
Pectin

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Lignin
Agar
Gum
Hemicellulose

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Carbohydrates are components of

Mucoproteins

Glycoproteins

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Glycolipids
Blood Group Substances
Structural and other functional

roles of Carbohydrates :

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Mucoproteins present in connective

tissues provide structural and

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mechanical support.

Hyaluronic acid in synovial fluid of

joints and vitreous humor of eye

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serve as lubricant and shock

absorbent.

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Heparin serves as natural

anticoagulant and prevents

intravascular coagulation.

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Heparan sulfate present on cell

surfaces act as recognition elements on

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cell membrane ,cell receptors and

helps in cell growth, cell adhesion,

and cell-cell communication.

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Pentose sugars Ribose and

Deoxyribose are

components of Nucleotides

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which build Nucleic acids ?

RNA and DNA and other

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nucleotide coenzymes.

Diagnostic Value of Carbohydrate:

Fructosan Inulin, is used

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to carry out:

Inulin Clearance Test,

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which checks, Glomerular

Filtration Rate of kidney.
Therapeutic Value of

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Carbohydrates:



Cardiac Glycosides Digoxin- used

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to treat cardiac insufficiency.

Glycosides Ovabain and Phlorizin

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- used in treatment of Diabetes

mellitus.

Glycosides Streptomycin

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,Erythromycin used to treat

bacterial infections.

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Mannitol- Serve as osmotic diuretic used

in treatment of Acute Renal Failure.

Lactulose -Relives Hyperammonaemia in

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patients of Hepato Encephalopathy.

Dextran- Used as plasma substitute in

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hemorrhagic cases to prevent

hypovoluemic shock.

Hyaluronan- Used to treat osteoarthritis.

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Sr.No

Reducing Sugars

Non Reducing Sugars

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1.

Reducing sugars possess free or potential

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Non reducing sugars does not

aldehyde or ketone group in it's structure

possess free or potential

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aldehyde or ketone group in it's
structure

2.

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Reducing sugars show reducing property .It

Non Reducing Sugars does not

form an Enediol in alkaline medium which then

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show reducing property.

reduces certain metallic ions of copper, bismuth

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3.

Reducing Sugars answers following tests

Non Reducing Sugars answers

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positively- Benedicts, Fehlings,

following tests negatively-

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Nylanders, Osazone Tests

Benedicts, Fehlings, Nylanders,
Osazone Tests

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4.

Reducing sugars exhibit Mutarotation

Non Reducing sugars does not

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show Mutarotation

5.

Examples of Reducing Sugars -

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Examples of Non Reducing Sugars

All Monosaccharide's are Reducing Sugars- ex

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or

Ribose, Glucose, Fructose etc

Non reducing Disaccharide-

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Reducing Disaccharides-Lactose, Maltose

Sucrose, Trehalose.

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Sr. No

Amylose

Amylopectin

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1.

Starch granule contains 15-20% of

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Starch granule has 80-85% of

Amylose.

Amylopectin.

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2.

Amylose is soluble and present in

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Amylopectin is insoluble part present at

inner core of starch granule.

periphery of starch granule.

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3.

Amylose is a linear, unbranched

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Amylopectin is a branched structure,

structure composed of 200-1000

composed of more than 1000 D

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D Glucose units repeatedly linked by Glucose units linked by (1-4)
(1-4) glycosidic bonds

glycosidic bond in linear and (1-6)

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glycosidic bond at branching point,
which appears after every 25-30
Glucose residues.

4.

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Molecular weight of Amylose is

Molecular weight of Amylopectin is 1

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400000

million .

5.

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Amylose gives blue color with Iodine Amylopectin gives reddish violet color
test.

with Iodine test.

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Sr. No

Starch

Glycogen

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Starch is Plant Homopolysaccharide. Glycogen is animal Homopolysaccharide

1.
2.

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Starch is composed of Amylose

Glycogen is highly branched structure

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(Linear)&Amylopectin (Branched)

with branching points appearing after
every 8-10 Glucose residues.

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3.

Starch is composed of 4000-7000

Glycogen is composed of 6000-30,000

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Glucose units.

Glucose units.

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4.

Starch is a storage form of Glucose Glycogen is storage form of Glucose and
and reserve food material in plants.

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reserve form of energy in animals and
human beings.

5.

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Starch is stored in roots, tubers,

Glycogen is stored in Liver and Muscles

seeds, raw fruits of plants.

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of animal and human body.

6.

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Starch serves as predominant dietary Glycogen is dietary form of Carbohydrate
Carbohydrate form in Veg and

only in Non-Veg eaters.

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Nonveg eaters.

7.

Starch with Iodine test gives deep

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Glycogen with Iodine test gives deep red

Blue color.

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color.

Sr.No

Starch

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Cellulose

Starch is a Glucosan composed of

--- Content provided by‍ FirstRanker.com ---

Cellulose is a Glucosan composed of

1.

DGlucose units, repeatedly linked DGlucose units ,repeatedly linked by

--- Content provided by‌ FirstRanker.com ---

by (1-4) glycosidic bonds in linear (1-4) glycosidic bonds.
and (1-6) glycosidic bonds at
branching point.

2.

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Starch present in plants serve as

Cellulose present in plant cell wall

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storage form of Glucose and reserve provides structural frame work to plants.
food material.

3.

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Dietary Starch is digested in human Dietary Cellulose is not digested in
GIT by enzyme Amylase.

human GIT due to absence of enzyme
Cellulase.

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4.

Starch has dietary and calorific value. Cellulose has no calorific value but serve

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as dietary fiber.
Sr.No

Dextrin

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Dextran

1.

Dextrin is plant

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Dextran is bacterial

Homopolysaccharide

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Homopolysaccharide.

2.

Dextrin is an intermediary

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Dextran is a Glucosan obtained

hydrolytic product of Starch from Sucrose solution incubated
digestion.

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with Leuconostoc mesenteroides.

3.

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Structure of Dextrin contains Structure of Dextran contains (1-
(1-4) & (1-6) glycosidic 3),
bonds.

(1-4) & (1-6) glycosidic bonds.

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4.

Dextrin solution is used in

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Dextran solution is used as plasma

commercially prepared infant volume expander in relieving
feedings and mucilage for

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hypovoluemic shock in cases of

pasting stamps.

hemorrhage.

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QUESTIONS
Q.1.Define carbohydrates.
Enumerate the biomedically

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important Carbohydrates.

Q.2.Classify and sub classify

Carbohydrates with terms and suitable

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examples.

Q.3. Simple Sugars /Monosaccharides

--- Content provided by FirstRanker.com ---

Q.4. Define stereoisomerism.

Enumerate the stereoisomers of

Glucose.

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Q.5. D & L Glucose
Q.6. Anomers
Q.7. Epimers
Q.8. What is optical activity? Give

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its type.

Q.9. Mutarotaion
Q.10. Chemical Reactions of Glucose

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/Reactions of Monosaccharides.

Q.11. Write 8 derivatives of

--- Content provided by​ FirstRanker.com ---

Monosaccharides and their

importance.

Q.12. Glycosides and its

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importance.

Q.13. Osazones Reaction. Draw the

--- Content provided by FirstRanker.com ---

structures of Glucosazone,

Fructosazone, Lactosazone &

Maltosazone.

--- Content provided by⁠ FirstRanker.com ---


Q.14. Explain why Glucosazone &

Fructosazone show same shape.
Q.15. Disaccharides (Definition,

--- Content provided by‍ FirstRanker.com ---


Types, Components, Glycosidic bonds,

Sources ,Biomedical Importances).

--- Content provided by⁠ FirstRanker.com ---

Q.16. Invert sugar,
Q.17. Lobry- de- Bruyn- Von

Ekenstein transformation

--- Content provided by​ FirstRanker.com ---

Q.18. Haworth and Fischer's projection of

Glucose.

Q.19. Why sucrose is a non-reducing

--- Content provided by​ FirstRanker.com ---


sugar?

Q.20. Homoglycans /

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Homopolysaccharides

Q.21. Glycosaminoglycans /Acid

Mucopolysaccharides (structure &

--- Content provided by⁠ FirstRanker.com ---


function)/Animal Heteropolysaccharides

Q.22. Inulin and its importance.
Q.23. Differences between

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Dextrin and Dextran.

Q.24. Cellulose & its

--- Content provided by​ FirstRanker.com ---

importance.

Q.25. Distinguish between

reducing & non-reducing sugars.

--- Content provided by‍ FirstRanker.com ---


Q.26. Write the components and

glycosidic linkages involved in following

--- Content provided by‌ FirstRanker.com ---

carbohydrates.

Sucrose
Maltose
Lactose

--- Content provided by FirstRanker.com ---

Glycogen
Cellulose
Amylose
Amylopectin
Q.27. Mucoproteins.

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Q.29. Biomedical Importance

of carbohydrates.

Q.30. Mucic acid test.

--- Content provided by​ FirstRanker.com ---

Q.31. Test's to check the

present of reducing sugars.

Q.32. Glycosidic bonds.

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Q.33. Glycoproteins of human body.
Q.34. Differentiate between Starch

and Cellulose.

--- Content provided by FirstRanker.com ---


Q.35 Differentiate between Starch

and Glycogen.

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Q.36 Mucopolysaccharidoses.
Q.37 Diagnostic and therapeutic

uses of Carbohydrates.

--- Content provided by FirstRanker.com ---


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