Download PTU B.Sc -MLS 3rd Semester 2019 May 48110 ANALYTICAL BIOCHEMISTRY Question Paper

Download PTU (I. K. Gujral Punjab Technical University) B-Sc MLS (B.Sc. Medical Laboratory Sciences) 3rd Semester 2019 May 48110 ANALYTICAL BIOCHEMISTRY Question Paper.

1 | M-48110 (S2)-1707
Roll No. Total No. of Pages : 02
Total No. of Questions : 09
B.Sc.(MLS) (2013 to 2017) (Sem.?3)
ANALYTICAL BIOCHEMISTRY
Subject Code : BMLS-305
M.Code : 48110
Time : 3 Hrs. Max. Marks : 60
INSTRUCTION TO CANDIDATES :
1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks
each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students
has to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students
has to attempt any TWO questions.

SECTION-A
1) Answer briefly :
a) Discuss Lambert?s law.
b) What is a standard?
c) What is a stationary phase?
d) What is monochromatic light?
e) What is ground state of an atom?
f) Discuss the applications of HPLC.
g) Name various parts of a spectrophotometer.
h) What is the significance of a control?
i) What do we mean by void volume?
j) Discuss principle of gel chromatography.
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1 | M-48110 (S2)-1707
Roll No. Total No. of Pages : 02
Total No. of Questions : 09
B.Sc.(MLS) (2013 to 2017) (Sem.?3)
ANALYTICAL BIOCHEMISTRY
Subject Code : BMLS-305
M.Code : 48110
Time : 3 Hrs. Max. Marks : 60
INSTRUCTION TO CANDIDATES :
1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks
each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students
has to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students
has to attempt any TWO questions.

SECTION-A
1) Answer briefly :
a) Discuss Lambert?s law.
b) What is a standard?
c) What is a stationary phase?
d) What is monochromatic light?
e) What is ground state of an atom?
f) Discuss the applications of HPLC.
g) Name various parts of a spectrophotometer.
h) What is the significance of a control?
i) What do we mean by void volume?
j) Discuss principle of gel chromatography.
2 | M-48110 (S2)-1707
SECTION-B
2) Write any six applications of electrophoresis and their advantages and disadvantages.
3) Explain principle, method and applications of ion exchange chromatography.
4) Give principle and limitations of Flame photometry.
5) Write a short note on hydrophobic interactions.
6) Explain principle, applications, advantages and disadvantages of using gas
chromatography.

SECTION-C
7) Define Electrophoresis. Explain PAGE and isoelectric focusing in detail.
8) Define Chromatography. Explain principle, application, advantages and disadvantages of
using column chromatography.
9) Explain Beer and Lambert?s law. Write at least six applications of colorimetry and
spectrophotometry. Put some words also about advantages of using spectrophotometer.









NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any
page of Answer Sheet will lead to UMC against the Student.
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