Max. Marks: 80 Marks
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PHARMACEUTICAL MICROBIOLOGY & BIOTECHNOLOGY
(Revised Scheme - 2)
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Q.P. CODE: 1957Your answers should be specific to the questions asked
Draw neat labeled diagrams wherever necessary
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LONG ESSAYS (Answer any Two)
2 x 10 = 20 Marks
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1.Define pure culture. What are the different methods used to isolate bacteria into pure
culture? Write the procedure for any one method.
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2.Classify various physical methods of sterilization giving examples. Write the principle
involved and the procedure for sterilization by radiation.
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3.Discuss briefly the application of recombinant DNA technique in the production of human
insulin.
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SHORT ESSAYS (Answer any Eight)8 x 5 = 40 Marks
4.
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Extrachromosomal genetic elements.
5.
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Risks involved in the use of r-DNA products.6.
Production of monoclonal antibody by hybridoma technique.
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7.
Antigen-Antibody reactions.
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8.Mode of Transmissions and treatment of cholera.
9.
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Classify bacteria based on nutritional and gases requirements giving examples.
10.
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Write the principle involved and the procedure for gram's staining.11.
Tyndallization.
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12.
How do you sterilize oily solutions & suspensions?
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13.Describe any one method for assessment of bacteriostatic activity.
SHORT ANSWERS
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10 x 2 = 20 Marks
14.
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Differential media.15.
Structure of antibody.
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16.
Distinguish disinfectants and antiseptic agents.
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17.Phenol co-efficient test.
18.
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Reproduction in bacteria.
19.
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Sterilization indicators.20.
Mechanism of action of phenol and alcohol as disinfecting agents.
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21.
Bacteriophase structure.
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22.Explain the terms `Bactericide' and `Beacteriostat'.
23.
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What diagnostic value the gram's staining reaction is? Give examples of gram positive and
gram negative organisms.
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