Max. Marks: 75 Marks
Pharmaceutical Microbiology
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Q.P. CODE: 5011
Your answers should be specific to the questions asked
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Draw neat labeled diagrams wherever necessaryLONG ESSAYS (Answer any Two)
2 x 10 = 20 Marks
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1.
What is pure culture? Enlist methods for isolation of pure culture. Describe any two
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industrially important techniques of preserving bacteria.(2+3+5)
2.
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Explain the principle, procedure and applications of sterilization using hot air oven. (3+5+2)
3.
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Explain different factors affecting disinfection.SHORT ESSAYS (Answer any Seven)
7 x 5 = 35 Marks
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4.
Outline working of TEM and SEM
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5.Define and classify culture media with examples.
6.
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Explain the principle involved in autoclaving.
7.
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Write principle and procedure of acid ?fast staining.8.
Write a note on cultivation of virus.
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9.
What are the main sources of contamination of an aseptic room? How will you prevent it?
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10.Explain principles involved in microbiological assay of vitamin B12.
11.
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Explain the different sources and types of microbial contamination of pharmaceuticals.
12.
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Write briefly the general procedure for cell culture.SHORT ANSWERS (Answer All)
10 x 2 = 20 Marks
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13.
Write contributions of Edward Jenner.
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14.Differentiate between flagella and fimbrae
15.
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Write bio-indicators for thermal sterilization.
16.
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Mention pharmaceutical uses of fungi.17.
Define disinfection.
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18.
Write the significance of positive control in sterility testing.
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19.Write the principle of membrane filtration method of sterility testing.
20.
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What is Grade 100?
21.
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List out different types of spoilage in pharmaceuticals.22.
What is primary cell culture?
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Document Outline
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