Code No. 7073
FACULTY OF SCIENCE
November / December 2017
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B.Sc. (CBCS) II - Semester Examination
Subject : BIOCHEMISTRY
Paper - II
Bioenergetics Biological Oxidations and Enzymology
Time : 3 hours
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Max. Marks : 80
Part— A (5 X 4 = 20 Marks)
(Short Answer Type)
Answer any Five of the following questions.
- Phosphate group transfer potential
- Exergonic and Endergonic reactions
- Standard reduction potential of two metabolic reactions
- Fo - F1 - ATPase
- Active site
- Transition state theory
- Acid-Base catalysis
- Ribozyme
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Part— B (4 X 15 = 60 Marks)
(Essay Answer Type)
Answer ALL questions from the following :
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- a) What are cytochromes and how are they classified? Discuss in detail about the structure and functions of cytochromes.
OR
Explain high energy compound. Add a note on energy transformation in living systems. - Write about various enzymes involved in biological oxidation reactions? Explain the Electron transport chain.
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Discuss the ultrastructure of mitochondria. Explain in detail the theories of oxidative phosphorylation. - Write the nomenclature and classification of enzymes with one example for each class.
OR
Define holoenzyme, apoenzyme, coenzyme and co-factor. Explain the methods of enzyme purification. - Explain Michaelis-Menten equation for single substrate reaction. Add a note on significance of Km and Vmax.
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OR
Explain how enzyme activity is regulated. Discuss ATCase as an allosteric enzyme.
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