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WBCs / Leukocytes
Learning Objectives
? Introduction to WBCs
? Myelopoiesis & its steps
? Neutrophil characteristics
? Inflammation and signs of inflammation
? Role of Neutrophil in
inflammation/infection
? Causes of Neutrophil number variation
Introduction ? WBCs / Leukocytes
Human body is exposed to a number of, diseases causing microbes all the time and
but body is equipped with the White blood cells to safeguards from these microbes.
We can say that WBCs are mobile units of body's protective mechanisms (IMMUNITY).
Peripheral blood - WBC count= 4000-11000/mm3, Life span 4-8 hours in bloodstream
Tissue life span estimated at 4-5 days.
Normal range of WBCs / Leukocytes
Total Leukocytes Count (TLC)
4000?11,000/mm3
Differential Leukocytes Count (DLC)
Absolute value % value
Neutrophils
3000?6000 50?70
Eosinophils
150?300
1?4
Basophils
o1?100 0.4
Lymphocytes
1500?4000
20?40
Monocytes
300?600
2?8
Introduction ? WBCs / Leukocytes
WBCs - concerned with non specific immunity- Granulocytes and Monocytes.
Neutrophils
Eosinophils
Basophils
WBCs - concerned with specific immunity - Lymphocytes
Myelopoiesis
Myeloid Stem Cells
HSC
Lymphoid Stem Cells
Myeloid Stem Cells CFU-GM Myeloblast Promyelocyte
Polymorphonuclear Band form Metamyelocyte Myelocyte
Neutrophil
Most Numerous of all leukocytes : 50 ? 70%
Diameter : 10 ? 14 ?
The Cytoplasm contains closely packed, fine and
violet-pink granules
The Nucleus can have 1 ? 6
Function- Neutrophil can cause effective `Phagocytosis' ?to
kill foreign particles and microbes.
Form first line of defense.
Neutrophil
Granules contents (biologically active substances) ?
Neutrophil - Role in Inflammation / Phagocytosis
Neutrophil blood cells are very important in
combating acute inflammation in body by the
process of
`leukocytes adhesion cascade' reaction i.e.:
Margination,
Rolling,
Adhesion,
Diapedesis
Chemotaxis and
Phagocytosis
Inflammation and Signs of inflammation
The five cardinal signs of inflammation--
Redness (rubor)
Heat (calor)
Swelling (tumor)
Pain (dolor)--were described by Cornelius Celsus.
A fifth consequence of inflammation is the loss of
function of the inflamed area, Rudolf Virchow
Neutrophil- Role in Inflammation / Phagocytosis
Phagocytosis- Phagesis: to eat, Cytos: cells
`Process of ingestion and destroying of foreign
microbes/antigens'
Endocytosis-Taking in Microbes and cell debris
Formation of Phagosome
lysosome
Phagolysosome
Neutrophil- Causes of variation in Number
Neutrophilia :Increase in neutrophil=>12000/mm3
Causes-
Neutropenia :Decrease in neutrophils= <2000/mm3
Causes-
Neutrophil- Leukemia
Leukaemia, is a malignant neoplasm that involve HSC
in the bone marrow and results in formation of high numbers of
abnormal nonfunctional white blood cells (>1 Lac/mm3). These white
blood cells are not fully developed and are called blasts or leukemia
cells.
Self Assessment
Normal WBC count is --------------------- /mm3
Steps of Myelopoiesis: ................. CFU-GM ................ Promyelocyte...................
Metamyelocyte ........................Mature form.
30% neutrophil cell nuclei of female contains a small, densely staining structure, consisting of a condensed,
inactive .....................known as ...........................
Name 3 important granules contents of neutrophil (biologically active substances)?...............
`leukocytes adhesion cascade' reactions include ......................................................
The inflammatory response involves three major stages: first, ...............................to increase blood flow;
second, ........................................... from the bloodstream; and third, .................................at the site of injury.
The five cardinal signs of inflammation are .................................................. of the inflamed area.
Name 6 important Released chemokines from injured tissues to mediate inflammation ? .....................
Thank you
This post was last modified on 08 April 2022