Max. Marks: 50 Marks
Faculty: Medical
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Subject: Biochemistry
PAPER ? II
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Q.P. CODE: M003Your answers should be specific to the questions asked.
Answer All the Following Questions
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10 X 05 = 50 Marks
1.
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How will you experimentally determine metabolic turnover of a substrate?2.
Differentiate between sensitivity and specificity of a test.
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3.
What are experimental approaches to differentiate apoptosis and necrosis?
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4.Mention briefly different types of PCR.
5.
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Define antibody specificity. Explain the principles of antigen-antibody interactions and the role of
law of mass action in antigen-antibody binding.
Discuss the advantages and disadvantages of utilizing molecular diagnostics in the clinical
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6.
laboratory.
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7.A single chain protein is having molecular weight of 69kDa. Calculate approximate number of
amino acids present in the protein.
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8.
What is POCT?
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9."Recombinant DNA technology is required for selective amplification of a particular gene" Explain.
10. Indicate the chemical composition and methods of separation of plasma lipoproteins
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Document Outline
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