Download JNTUK B.Pharm 2-1 2019 BP303T Pharmaceutical Microbiology Question Paper

Code No: BP303T

PCI

SET - 1

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II B. Pharmacy I Semester Supplementary Examinations, May - 2019

PHARMACEUTICAL MICROBIOLOGY

Time: 3 hours

Max. Marks: 75

Note: 1. Question paper consists of three parts (Part-I, Part-II & Part-III)

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2. Answer ALL (Multiple Choice) Questions from Part-I

3. Answer any TWO Questions from Part-II

4. Answer any SEVEN Questions from Part-III

PART-I

1. (i) Nagler's reaction is used for detection of (1M)
   1. Streptococci

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   2. Clostridia
   3. Bacillus
   4. None of these
2. (ii) For effective sterilization in an autoclave the temperature obtained is (1M)
   1. 50°C

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   2. 100°C
   3. 120°C
   4. 180°C
3. (iii) Agar is obtained from... (1M)
   1. Brown algae

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   2. Red algae
   3. Green algae
   4. Blue-green algae
4. (iv) The image observed with eye obtained in a compound microscope is... (1M)
   1. Real

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   2. Virtual
   3. Real inverted
   4. Virtual inverted
5. (v) Father of microbiology is... (1M)
   1. Louis Pasteur

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   2. Lister
   3. Antonie van Leeuwenhoek
   4. Robert Koch
6. (vi) Tuberculosis is a (1M)
   1. Water borne disease

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   2. Air borne disease
   3. Food borne disease
   4. Arthropod borne disease
7. (vii) The main feature of prokaryotic organism is (1M)
   1. Absence of locomotion

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   2. Absence of nuclear envelope
   3. Absence of nuclear material
   4. Absence of protein synthesis
8. (viii) Nitrites are oxidized to nitrates by the microorganism.. (1M)
   1. Nitrosomonas

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   2. Nitrosococcus
   3. Nitrobacter
   4. Azatobacter
9. (ix) bacteria uses chemical compounds as a source of energy (1M)
   1. Phototrophs

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   2. Autotrophs
   3. Chemotrophs
   4. Chemolithotroph
10. (x) Vitamin function as. (1M)
    1. Co-enzymes

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    2. Co-molecules
    3. Building blocks of cell
    4. None of these
11. (xi) The number of generations per hour in a bacteria is. (1M)
    1. Growth rate

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    2. Generation time
    3. Sigmoid curve
    4. None of these
12. (xii) Condensation of light in light Microscope is done by......... (1M)
    1. Objective

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    2. Condenser
    3. Ocular
    4. All of these
13. (xiii) Minimum growth temperature is.......... (1M)
    1. The growth of organisms at lowest temperature

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    2. The lowest temperature at which the microorganisms grow
    3. The maximum temperature at which the growth is stable
    4. None of these
14. (xiv) The utilization of light energy to drive the synthesis of ATP is called as....... (1M)
    1. Photolysis

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    2. Photophosphorylation
    3. Photosynthesis
    4. Respiration
15. (xv) Betalactam ring is present in... (1M)
    1. Erythromycin

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    2. Penicillin
    3. Tetracyclins
    4. Chloramphenicol
16. (xvi) Ciprofloxacin acts by inhibiting (1M)
    1. Cell wall synthesis

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    2. RNA synthesis
    3. Folate synthesis
    4. DNA gyrase
17. (xvii) Mantoux test detects (1M)
    1. M. tuberculosis

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    2. Cyanobacteria
    3. Clostridia
    4. Both a and b
18. (xviii) Common cold is caused by....... (1M)
    1. Adenovirus

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    2. Rhinovirus
    3. Hepatitis virus
    4. Pox virus
19. (xix) ......... is used for testing Diphtheria virulence. (1M)
    1. Ascoli's thermoprecipitation test

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    2. Elek's gel precipitation test
    3. C.R.P test
    4. M.R.T. test
20. (xx) Bacterial gastroenteritis is caused by (1M)
    1. Escherichia coli

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    2. Vibrio cholerae
    3. Clostridium difficile
    4. All the above

PART-II

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2. a) Write a note on nitrogen sources used in microbial media. (5M)
   b) Explain the methods used for preservation of microorganisms. (5M)
3. a) Write short notes on chemical methods of sterilization. (5M)
   b) With a neat sketch explain the principle and working of an autoclave. (5M)
4. a) Write principle and procedure involved in antibacterial activity evaluation using (5M) broth dilution method.
   

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   b) Discuss the components and working of vertical laminar airflow unit. (5M)

PART-III

5. Discuss the factors affecting bacterial growth. (5M)
6. Write short notes on significance of microbiology in pharmaceutical sciences. (5M)
7. Write in brief on biochemical tests used for identification of bacteria. (5M)

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8. Write short notes on sterilization by filtration. (5M)
9. Write methods used for cultivation of virus. (5M)
10. Explain the procedure involved in sterility testing of eye drops as per IP. (5M)
11. What is clean area? Write its significance. (5M)
12. Discuss the principle and procedure involved in microbiological assay of vitamin B12. (5M)

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13. Write short notes on media used for cell culture. (5M)

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