First Semester M. Hhéll:"rs Degree Examination
[Time: 3 Hours] [Max. Marks: 75]
Phytochemistry
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Q.P. CODE: 5132
Your answers should be specific to the questions asked.
Draw neat, labeled diagrams wherever necessary.
LONG ESSAY (Answer any Three) 3 X 10 = 30 Marks
- Describe the protocol for extraction for phytochemical screening of fresh and dry drugs. Explain the tests for identification of various phytoconstituents.
- Explain different in-vitro and in-vivo screening methods for antidiabetic activity.
- Discuss the use of grafting and mutant strains for elucidation of biosynthetic pathways.
- Describe the biosynthesis of ephedrine.
- Explain applications of LCMS and GCMS for phytochemical characterization of extracts.
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SHORT ESSAY (Answer any Nine) 9 X 5 = 45 Marks
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- Explain the biosynthesis of umbelliferone.
- Write a note on selection and optimization of lead anticancer compounds from natural sources with suitable examples.
- Explain the isolation, purification and characterization of piperine.
- Explain different screening methods available for evaluation of antilithiatic activity.
- Explain the principle and applications of flash-column chromatography.
- Describe the biosynthesis of strychnine.
- Write the procedure for isolation, purification and identification of cucurbitacins.
- Explain different screening methods for antiviral activity.
- Substantiate herbs as potential source for drug discovery.
- Write the importance of HPTLC in herbal-analysis.
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