Rajiv Gandhi University of Health Sciences
Second Semester M. Pharmacy Degree Examination
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[Time: 3 Hours] [Max. Marks: 75]
Advanced Spectral Analysis-III
Q.P. CODE: 5157
Your answers should be specific to the questions asked.
Draw neat, labeled diagrams wherever necessary.
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LONG ESSAY (Answer any Three) 3 X 10 = 30 Marks
- Explain the principle and working ATR-IR instrument.
- Outline the theory of 2D NMR spectroscopy.
- Explain the general fragmentation rules for organic compounds in mass spectroscopy.
- Write the principles, instrumentation and applications of LC-MS with special emphasis on interface.
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SHORT ESSAY (Answer any Nine) 9 X 5 = 45 Marks
- Explain Wood ward- Fieser rule for 1, 3-butadienes.
- How will you identify the following functional groups in an organic compound (-OH-NH,-COOH, C=C, -CHO) by IR spectroscopy.
- Explain in detail Mc. Lafferty rearrangement.
- Give brief account on NOESY.
- Brief account on HPTLC.
- Write the principle involved in TGA.
- Give a brief account on Radio immuno assay of digitalis.
- Explain the light sources and sample introduction system of AAS.
- Write the principle involved in Raman spectroscopy.
- How flash chromatography differ from traditional column chromatography.
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