Download RGUHS (Rajiv Gandhi University of Health Sciences ) Bachelors in Science in Medical Laboratory Technology (B.Sc MLT) 2015 April 2nd Year 3156 Biochemistry II Previous Question Paper

Rajiv Gandhi University of Health Sciences, Karnataka
II Year B.Sc. (M.L.T) Degree Examination ? APRIL 2015
Time: Three Hours


Max. Marks: 80 Marks
BIOCHEMISTRY ? II
Q.P. CODE: 3156
Your answers should be specific to the questions asked.
Draw neat labeled diagrams wherever necessary
LONG ESSAYS (Answer any Two)
2 x 10 = 20 Marks
1.
Describe the chemistry, sources, RDA, functions and deficiency manifestations of "Vitamin C".
2.
What are "clearance tests"? Give a detailed account of the procedure for carrying out the
endogenous creatinine clearance test. Add a note on the biological reference intervals and the
clinical significance.
3.
Explain the principle of Spectrophotometry. Draw a schematic diagram of a single ? beam
spectrophotometer. Add a note on standardization of Spectrophotometer.
SHORT ESSAYS (Answer any Six)
6 x 5 = 30 Marks
4.
Types of water in a laboratory. Discuss the NCCLS specification for laboratory water.
5.
What are coenzymes? Discuss the salient features of coenzymes. Add a note on
metalloenzymes.
6.
Explain the preparation of a calibration curve citing an example. Add a note on the linearity of
the method based on the calibration curve.
7.
Discuss Gouty arthritis in detail. Give the principle for the estimation of uric acid.
8.
Explain the tissue distribution and functions of chondroitin sulfate and heparin.
9.
Explain the tertiary structures of proteins citing an example.
10.
Urea cycle and energetics.
11.
Procedure for ammonium chloride loading test and interpretation.
SHORT ANSWERS (Answer any Ten)
10 x 3 = 30 Marks
12.
Anaplerotic role of TCA cycle.
13.
Types of RNA
14.
Albuminometer and Urinometer.
15.
Preparation of protein free filtrate for quantitative estimations-Glucose by Reductometric method
and Creatinine by Jaffe's method in blood.
16.
What is meant by Timed sample collection? Explain with one example each under serum and
urine.
17.
Name three glycogen storage disorders and the enzyme associate with it.
18.
Discuss different sample collection tubes used during phlebotomy. Add a note on the order of
draw during multiple specimen collection.
19.
Functions of DNA
20.
Classification of proteins based on nutritional value.
21.
Inhibitors of glycolysis.
22.
Microalbuminuria.
23.
Riboflavin
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