Rajiv Gandhi University of Health Sciences, Karnataka
II Year B.Sc. (M.L.T) Degree Examination - APRIL 2015
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Time: Three Hours Max. Marks: 80 Marks
BIOCHEMISTRY —1I
Q.P. CODE: 3156
Your answers should be specific to the questions asked.
Draw neat labeled diagrams wherever necessary
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LONG ESSAYS (Answer any Two) 2 x 10 = 20 Marks
- Describe the chemistry, sources, RDA, functions and deficiency manifestations of “Vitamin C”.
- What are “clearance tests”? Give a detailed account of the procedure for carrying out the endogenous creatinine clearance test. Add a note on the biological reference intervals and the clinical significance.
- Explain the principle of Spectrophotometry. Draw a schematic diagram of a single - beam spectrophotometer. Add a note on standardization of Spectrophotometer.
SHORT ESSAYS (Answer any Six) 6 x 5 = 30 Marks
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- Types of water in a laboratory. Discuss the NCCLS specification for laboratory water.
- What are coenzymes? Discuss the salient features of coenzymes. Add a note on metalloenzymes.
- Explain the preparation of a calibration curve citing an example. Add a note on the linearity of the method based on the calibration curve.
- Discuss Gouty arthritis in detail. Give the principle for the estimation of uric acid.
- Explain the tissue distribution and functions of chondroitin sulfate and heparin.
- Explain the tertiary structures of proteins citing an example.
- Urea cycle and energetics.
- Procedure for ammonium chloride loading test and interpretation.
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SHORT ANSWERS (Answer any Ten) 10 x 3 = 30 Marks
- Anaplerotic role of TCA cycle.
- Types of RNA
- Albuminometer and Urinometer.
- Preparation of protein free filtrate for quantitative estimations-Glucose by Reductometric method and Creatinine by Jaffe’s method in blood.
- What is meant by Timed sample collection? Explain with one example each under serum and urine.
- Name three glycogen storage disorders and the enzyme associate with it.
- Discuss different sample collection tubes used during phlebotomy. Add a note on the order of draw during multiple specimen collection.
- Functions of DNA
- Classification of proteins based on nutritional value.
- Inhibitors of glycolysis.
- Microalbuminuria.
- Riboflavin
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This download link is referred from the post: RGUHS B.Sc MLT Last 10 Years 2012-2022 Question Papers || Rajiv Gandhi University of Health Sciences
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