Download RGUHS M.Sc MLT 2019 April 1st Year 9811 Biochemistry I Revised Scheme 2 Question Paper

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University of Health Sciences

First Year M.Sc. Medical Biochemistry Examination

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[Time: 3 Hours] [Max. Marks: 100]

Biochemistry - I

(Revised Scheme - 2)

Q.P. CODE: 9811

Your answers should be specific to the questions asked.

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Draw neat, labeled diagrams wherever necessary.

Section A and section B are to be written in same Answer Book

Section A - Clinical Biochemistry [50 marks]

LONG ESSAY 1 X 20 = 20 Marks

1. Give an account of urine collection in a laboratory and its preservation, storage, transport and disposal. Add a note on physical and chemical examination of urine samples.

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SHORT ESSAY (Attempt any six) 6 X 5 = 30 Marks

1. Write a note on BMR and the factors affecting it.
2. Classification of enzymes with examples.
3. Lipid profile and coronary artery diseases
4. Acid base disorders

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5. Clinical significance of creatinine. Write the principle of estimation.
6. Explain competitive and non-competitive enzyme inhibition with examples.
7. Note on oral glucose tolerance test
8. Structural organisation

Section B - Laboratory Principles & Techniques [50 marks]

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LONG ESSAY 1 X 20 = 20 Marks

1. Write the principle of centrifugation. Discuss in detail on the types and applications of centrifuges. Add a note on cell fractionation.

SHORT ESSAY (Attempt any six) 6 X 5 = 30 Marks

1. Potentiometry
2. Agarose Gel electrophoresis

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3. Use, care and maintenance of analytical weighing balance
4. Principle and applications of affinity chromatography
5. Cleaning of glasswares
6. Quality control in a laboratory
7. Write about general safety laboratory practice.

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8. Principle and applications of colorimetry

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