Download MBBS TMU 2nd Year 2017 MBS202 Microbiology I Question Paper

MBBS II (Second) Professional Examination 2016-17

Course Code:MBS202 Paper ID: 0322405

Microbiology-I

Time: 2 Hours 40 Minutes Max Marks: 30

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Note: Attempt all questions. Draw proper diagrams to support your answer.

Part 'B'

1. A 21 years old female comes with fever, increase frequency and burning micturation. (1+4+2=7)
   1. Enumerate the bacterial causes for this condition.
   2. Discuss the laboratory diagnosis of this condition.
   3. Name the antibiotics can be used to treat this condition

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2. Define sterilization. What are the different methods for sterilization? Give a note on autoclave. (7)
3. Write short notes on: (8+8)
   1. ELISA
   2. Laboratory diagnosis of enteric fever.

Roll No.

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Student's Signature Student's Name

Course Code: MBS202 Paper ID: 0322405

Microbiology-I

Part 'A'

Time: 20 Minutes Max Marks: 10

Note: 1. Attempt all questions and return this part of the question paper to the invigilator after 20 Minutes.

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2. Please tick (√) correct one only. Cutting, overwriting or any other marking are not allowed.

3. For answering please use Ball- pen only.

1. Q.1 Negative staining is done for demonstration of:
   1. Flagella
   2. Cell wall
   3. Capsule

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   4. Endospore
2. Q.2 Differential stains are:
   1. Gram stain
   2. Acid-Fast stain
   3. Both

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   4. None of the above
3. Q.3 Which of the following endospores:
   1. Autoclave
   2. Incineration
   3. Hot air sterilization

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   4. Pasteurization
4. Q.4. Which of the following is best to sterilize heat labile solutions:
   1. Dry heat
   2. Autoclave
   3. Membrane filtration

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   4. Pasteurization
5. Q.5 Which of the following is bactericidal:
   1. Membrane filtration
   2. Ionizing radiation
   3. Freeze-drying

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   4. Deep freezing
6. Q.6 An organism grown on nutrient agar shows green colour pigment is likely to be:
   1. Staphylococcus
   2. E.coli
   3. Pseudomonas

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   4. Peptostreptococcus
7. Q.7 Most Common organism causing nosocomial infection is hospital acquired pneumonia:
   1. Pseudomonas
   2. Proteus
   3. Vibrio

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   4. Mycoplasma
8. Q.8 Urease producing all except:
   1. H. pylori
   2. E.coli
   3. Klebsilla

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   4. Proteus
9. Q.9 Mc Conkey medium is an example of:
   1. Transport medium
   2. Differential Medium
   3. Selective medium

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   4. Simple medium
10. Q.10 Metachromatic granules can be seen by:
    1. Albert's stain
    2. Gram stain
    3. ZN Stain

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    4. None of the above

P.T.O.

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11. Q.11 Which bacteria utilizes citrate:
    1. E.coli

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    2. Klebsiella
    3. Shigella
    4. Clostridium
12. Q.12 The following are true about culture media for microbes except:
    1. Lowensten-Jensen is used to isolate mycobateria

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    2. Thioglycolate broth allows only anaerobes to grow
    3. MacConkey agar prevents the growth of Gram negative bacteria
    4. Sabouraud's culture is useful for culturing fungal infection
13. Q.13 The process of destroying microbes including spores is called:
    1. Disinfection

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    2. Antisepsis
    3. Sterilization
    4. None
14. Q.14 The sterilization of culture media containing serum:
    1. Autoclaving

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    2. Gamma radiation
    3. Micropore filter
    4. inspissations
15. Q.15 The following substances are used in Gram staining except:
    1. Iodine

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    2. Crystal violet
    3. Safranin
    4. Silver stain
16. Q.16 What oil is used for oil immersion lens:
    1. Mustard oil

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    2. Cedar wood oil
    3. Glycerin
    4. None of the above
17. Q.17 The following staphylococci are coagulase positive:
    1. S. aureus

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    2. S. epidermidis
    3. S. saprophyticus
    4. Hyicus
18. Q.18 The Nagler reaction helps in identification of:
    1. E.coli

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    2. Klebsiella
    3. Proteus
    4. Clostridium
19. Q.19 Temperature of hot air oven is:
    1. 160°C for 2 hours

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    2. 170°C for 2 hours
    3. 180°C for 2 hours
    4. 121°C for 15 min
20. Q.20 Lawn culture method used for:
    1. Antibiotic Sensitivity Testing

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    2. For isolation of bacteria
    3. None
    4. A& B both
21. Q.18 The Nagler reaction helps in identification of:

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