Download OU B.Sc 2016 April 2nd Year 2049 Biohemistry Question Paper

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FACULTY OF SCIENCE

B.Sc. II Year Examination, March / April 2016

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Subject : BIOCHEMISTRY

Paper - II : Metabolism and Biochemical Techniques

Time : 3 hours Max. Marks : 100

Part — A (8 X 5 = 40 Marks)

(Short Answer Type)

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Note : Answer any Eight from the following.

1. Chemiosmotic theory
2. Substrate level phosphorylation
3. Biological oxidations
4. Light reactions of photosynthesis

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5. Anaplerotic reactions
6. Brief outline of the biosynthesis of triacylglycerol
7. Decarboxylation and deamination reactions of amino acids
8. Inborn errors of branched chain amino acids
9. Centrifugation techniques

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10. Tracer techniques
11. Ion exchange chromatography

Part- B (4 X 15 = 60 Marks)

(Essay Answer Type)

Note : Answer all the questions.

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1. a) Describe the ultrastructure of mitochondria and explain the electron transport mechanism.
   OR
   b) Write about energy transformations, free energy concept and phosphate group transfer potential.
2. a) Explain citric acid cycle. Comment on the fate of pyruvate.
   OR
   

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   b) Discuss beta-oxidation of fatty acids.
3. a) Discuss the metabolic fate of glycine, serine and methionine.
   OR
   b) Explain the de novo biosynthesis of purines. Write down the cause for Lesch-Nyhan syndrome.
4. a) Give an account of principle and methodology of gel filtration, paper and affinity chromatographic methods.
   

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   OR
   b) Explain the Beer — Lambert law, principle and methods of colorimetry and spectrophotometry.

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