Enrolment No.
GUJARAT TECHNOLOGICAL UNIVERSITY
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BE - SEMESTER- III (New) EXAMINATION — WINTER 2019
Subject Code: 3131302 Date: 26/11/2019
Subject Name: Environmental Microbiology & Bioremediation
Time: 02:30 PM TO 05:00 PM Total Marks: 70
Instructions:
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- Attempt all questions.
- Make suitable assumptions wherever necessary.
- Figures to the right indicate full marks.
Q.1 (a) Give the classification of bacteria. 03
(b) Write a short note on Whittaker five kingdom classification. 04
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(c) Explain the role of microbes in human life and in environment. 07
Q.2 (a) Differentiate between Animal Cell & Plant Cell. 03
(b) Give different stages of bacterial growth Curve and explain about each stage. 04
(c) Explain the complete procedure to perform MPN test for water. 07
OR
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(c) Briefly explain the SPC test of milk using serial dilution techniques. 07
Q.3 (a) Give the classification of Microscopes. 03
(b) Explain the working of electron microscope with neat sketch. 04
(c) Enlist the diseases caused by protozoa and explain the symptoms, treatment and prevention of any one in detail. 07
OR
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Q.3 (a) Describe the working principle of Transmission Electron Microscopy. 03
(b) Differentiate between Light Microscope & Electron Microscope. 04
(c) Explain the procedure for production of Lactic Acid. 07
Q.4 (a) Differentiate between Disinfection & Sterilization. 03
(b) Compare Gram Positive bacteria with Gram Negative bacteria. 04
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(c) Enlist and explain physical agents for control of Microbes. 07
OR
Q.4 (a) Differentiate between Pasteurization & Lyophilization. 03
(b) List out the different staining techniques and explain gram staining in detail. 04
(c) Write a short not on Chemical agents for the control of microbes. 07
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Q.5 (a) List out the various cultural methods. 03
(b) Differentiate between biodegradation and bioremediation. 04
(c) Discuss the advantages and disadvantages of In-situ Bioremediation. 07
OR
Q.5 (a) Describe a list of different techniques used for preservation of pure culture. 03
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(b) Differentiate between In-situ Bioremediation and Ex-situ Bioremediation 04
(c) Write a short note on Phytoremediation. 07
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