Download GTU (Gujarat Technological University) BE/BTech (Bachelor of Engineering / Bachelor of Technology) 2019 Winter 3rd Sem New 3131407 Basic Microbiology Previous Question Paper
GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER ? III (New) EXAMINATION ? WINTER 2019
Subject Code: 3131407 Date: 3/12/2019
Subject Name: Basic Microbiology
Time: 02:30 PM TO 05:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Figures to the right indicate full marks.
3. Draw neat illustrated diagrams wherever necessary.
4. Write scientific names as per the norms
MARKS
Q.1 (a) Draw a flowchart to depict the scheme of five kingdom
classification based on characteristics.
03
(b) Tabulate the differences between eukaryotic and prokaryotic
cell alongwith diagram and also give examples.
04
(c) Describe the difference between gram +ve and gram ?ve
bacteria, staining reaction, and diagram of respective cell wall.
Give example of gram +ve rod, gram +ve cocci, gram ?ve rod,
gram ?ve cocci
07
Q.2 (a) Write a short note on chemical and physical methods to
control microorganisms
03
(b) Draw a flowchart to depict Koch postulates. What is the
significance of Koch postulates?
04
(c) Draw a diagram depicting steps of ELISA. Explain a) ELISA
is a semiquantitative assay, b) application of ELISA c) types
of ELISA
07
OR
(c) Justify the statement ?Louis Pasteur is aptly known as Father
of Microbiology
07
Q.3 (a) Draw a diagram to depict Holliday junction. How does it help
in evolution?
03
(b) Microbial growth occurs is observed as sigmoidal curve.
Describe each phase of the sigmoidal curve. During which
phase the primary metabolites are formed?
04
(c) Describe PCR, its applications, advantages and
disadvantages. Draw a diagram to depict PCR cycle and enlist
the ingredients used for PCR
07
OR
Q.3 (a) Enlist the significance of microorganisms in agricultural
microbiology
03
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Seat No.: ________ Enrolment No.___________
GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER ? III (New) EXAMINATION ? WINTER 2019
Subject Code: 3131407 Date: 3/12/2019
Subject Name: Basic Microbiology
Time: 02:30 PM TO 05:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Figures to the right indicate full marks.
3. Draw neat illustrated diagrams wherever necessary.
4. Write scientific names as per the norms
MARKS
Q.1 (a) Draw a flowchart to depict the scheme of five kingdom
classification based on characteristics.
03
(b) Tabulate the differences between eukaryotic and prokaryotic
cell alongwith diagram and also give examples.
04
(c) Describe the difference between gram +ve and gram ?ve
bacteria, staining reaction, and diagram of respective cell wall.
Give example of gram +ve rod, gram +ve cocci, gram ?ve rod,
gram ?ve cocci
07
Q.2 (a) Write a short note on chemical and physical methods to
control microorganisms
03
(b) Draw a flowchart to depict Koch postulates. What is the
significance of Koch postulates?
04
(c) Draw a diagram depicting steps of ELISA. Explain a) ELISA
is a semiquantitative assay, b) application of ELISA c) types
of ELISA
07
OR
(c) Justify the statement ?Louis Pasteur is aptly known as Father
of Microbiology
07
Q.3 (a) Draw a diagram to depict Holliday junction. How does it help
in evolution?
03
(b) Microbial growth occurs is observed as sigmoidal curve.
Describe each phase of the sigmoidal curve. During which
phase the primary metabolites are formed?
04
(c) Describe PCR, its applications, advantages and
disadvantages. Draw a diagram to depict PCR cycle and enlist
the ingredients used for PCR
07
OR
Q.3 (a) Enlist the significance of microorganisms in agricultural
microbiology
03
(b) Write a descriptive note on IMViC Test. Write the IMViC
reaction for any two microorganisms
04
(c) A butcher, asymptomatic carrier of Salmonella, minced meat
without wearing gloves. As consequence, the meat was
contaminated with 25 cells of Salmonella spp. and 32 cells of
S. enterica. Taking into account that the generation time of
Salmonella spp. is 30 minutes and its lag phase is 3 h, and that
the specific growth rate constant of S. enterica using meat as
substrate is 0.17 h
-1
and its lag phase lasts 5 h, calculate the
number of Salmonella cells that will be present in the meat 10
hours after being prepared.
07
Q.4 (a) Explain the formation of HFr and F? strains formed during
bacterial conjugation. Also write about the significance of
?Merozygote?
03
(b) In a picnic, the paella was contaminated with 46 cells of
Propionibacterium acnes. If P. acnes has a generation time of
90 minutes and a lag phase of 1.5 h, how many cells of this
bacterium will be present in the paella after 10 h?
04
(c) Bacteria change from non-pathogenic strain to pathogenic
strain. Explain the concept with experiment conducted by
Griffith. Also suggest an application in reference to food
industry.
07
OR
Q.4 (a) Draw a diagram to depict parts of compound bright field
microscope.
03
(b) Give example of microorganisms 1) acid fast bacteria 2)
photoautotrophic bacteria 3) bacteria producing bacteriocin 4)
fungi used for commercial production of penicillin
04
(c) Explain DNA replication. Why lagging strand is named as
such? Enlist the name and functions of all enzymes and
proteins involved in DNA replication.
07
Q.5 (a) Explain replica plating method for isolation of auxotroph.
Explain the term bradytrophic bacteria.
03
(b) Draw labelled diagram to depict arrangement of flagella in
bacteria
04
(c) Draw a diagram to depict transduction. What is lytic and
lysogenic cycle? Explain the term specialized transduction.
07
OR
Q.5 (a) A blue cheese sample was crushed in saline and serially
diluted upto 10
-7
. The last dilution when spread plated resulted
in 200 colonies. What should be interpreted by the analyst
regarding the cell count in terms of log cfu/ml? Can you
speculate the results in plates treated similarly for 10
-6
and
10
-8
dilutions
03
FirstRanker.com - FirstRanker's Choice
Seat No.: ________ Enrolment No.___________
GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER ? III (New) EXAMINATION ? WINTER 2019
Subject Code: 3131407 Date: 3/12/2019
Subject Name: Basic Microbiology
Time: 02:30 PM TO 05:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Figures to the right indicate full marks.
3. Draw neat illustrated diagrams wherever necessary.
4. Write scientific names as per the norms
MARKS
Q.1 (a) Draw a flowchart to depict the scheme of five kingdom
classification based on characteristics.
03
(b) Tabulate the differences between eukaryotic and prokaryotic
cell alongwith diagram and also give examples.
04
(c) Describe the difference between gram +ve and gram ?ve
bacteria, staining reaction, and diagram of respective cell wall.
Give example of gram +ve rod, gram +ve cocci, gram ?ve rod,
gram ?ve cocci
07
Q.2 (a) Write a short note on chemical and physical methods to
control microorganisms
03
(b) Draw a flowchart to depict Koch postulates. What is the
significance of Koch postulates?
04
(c) Draw a diagram depicting steps of ELISA. Explain a) ELISA
is a semiquantitative assay, b) application of ELISA c) types
of ELISA
07
OR
(c) Justify the statement ?Louis Pasteur is aptly known as Father
of Microbiology
07
Q.3 (a) Draw a diagram to depict Holliday junction. How does it help
in evolution?
03
(b) Microbial growth occurs is observed as sigmoidal curve.
Describe each phase of the sigmoidal curve. During which
phase the primary metabolites are formed?
04
(c) Describe PCR, its applications, advantages and
disadvantages. Draw a diagram to depict PCR cycle and enlist
the ingredients used for PCR
07
OR
Q.3 (a) Enlist the significance of microorganisms in agricultural
microbiology
03
(b) Write a descriptive note on IMViC Test. Write the IMViC
reaction for any two microorganisms
04
(c) A butcher, asymptomatic carrier of Salmonella, minced meat
without wearing gloves. As consequence, the meat was
contaminated with 25 cells of Salmonella spp. and 32 cells of
S. enterica. Taking into account that the generation time of
Salmonella spp. is 30 minutes and its lag phase is 3 h, and that
the specific growth rate constant of S. enterica using meat as
substrate is 0.17 h
-1
and its lag phase lasts 5 h, calculate the
number of Salmonella cells that will be present in the meat 10
hours after being prepared.
07
Q.4 (a) Explain the formation of HFr and F? strains formed during
bacterial conjugation. Also write about the significance of
?Merozygote?
03
(b) In a picnic, the paella was contaminated with 46 cells of
Propionibacterium acnes. If P. acnes has a generation time of
90 minutes and a lag phase of 1.5 h, how many cells of this
bacterium will be present in the paella after 10 h?
04
(c) Bacteria change from non-pathogenic strain to pathogenic
strain. Explain the concept with experiment conducted by
Griffith. Also suggest an application in reference to food
industry.
07
OR
Q.4 (a) Draw a diagram to depict parts of compound bright field
microscope.
03
(b) Give example of microorganisms 1) acid fast bacteria 2)
photoautotrophic bacteria 3) bacteria producing bacteriocin 4)
fungi used for commercial production of penicillin
04
(c) Explain DNA replication. Why lagging strand is named as
such? Enlist the name and functions of all enzymes and
proteins involved in DNA replication.
07
Q.5 (a) Explain replica plating method for isolation of auxotroph.
Explain the term bradytrophic bacteria.
03
(b) Draw labelled diagram to depict arrangement of flagella in
bacteria
04
(c) Draw a diagram to depict transduction. What is lytic and
lysogenic cycle? Explain the term specialized transduction.
07
OR
Q.5 (a) A blue cheese sample was crushed in saline and serially
diluted upto 10
-7
. The last dilution when spread plated resulted
in 200 colonies. What should be interpreted by the analyst
regarding the cell count in terms of log cfu/ml? Can you
speculate the results in plates treated similarly for 10
-6
and
10
-8
dilutions
03
(b) Explain biosafety levels in terms of lethalness of
pathogenicity and availability of cure. Name any 4 centers in
India where biosafety level-4 can be handled. Give examples
of ways/ organisms used for bioterrorism during ancient
times/ currently
04
(c) Give reason for the following (briefly; diagram not required)
i. Swan neck experiment was the ultimate proof for
biogenesis
ii. The credit for vaccination goes to Edward Jenner and
not Lady Mary Wortley Montagu
iii. Acid fast staining is a differential staining method
iv. Conventional DNA polymerase cannot be used for
PCR
v. Even after being heat treated bacteriocin results in
antimicrobial effect
vi. 70% methanol is more effective in killing
microorganisms than 100% methanol
vii. Oil immersion is necessary when 100X objective lens
is used during microscopy
07
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This post was last modified on 20 February 2020