GTU BE/B.Tech 2019 Winter Question Papers || Gujarat Technological University
Subject Code: 3131407 Date: 3/12/2019
Subject Name: Basic Microbiology
Time: 02:30 PM TO 05:00 PM Total Marks: 70
Seat No.: Enrolment No.
GUJARAT TECHNOLOGICAL UNIVERSITY
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BE - SEMESTER- III (New) EXAMINATION — WINTER 2019Subject Code: 3131407 Date: 3/12/2019
Subject Name: Basic Microbiology
Time: 02:30 PM TO 05:00 PM Total Marks: 70
Instructions:
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- Attempt all questions.
- Figures to the right indicate full marks.
- Draw neat illustrated diagrams wherever necessary.
- Write scientific names as per the norms
| MARKS | |
|---|---|
| Q.1 | |
| (a) Draw a flowchart to depict the scheme of five kingdom classification based on characteristics. | 03 |
| (b) Tabulate the differences between eukaryotic and prokaryotic cell along with diagram and also give examples. | 04 |
| (c) Describe the difference between gram +ve and gram –ve bacteria, staining reaction, and diagram of respective cell wall. Give example of gram +ve rod, gram +ve cocci, gram –ve rod, gram –ve cocci | 07 |
| Q.2 | |
| (a) Write a short note on chemical and physical methods to control microorganisms | 03 |
| (b) Draw a flowchart to depict Koch's postulates. What is the significance of Koch's postulates? | 04 |
| (c) Draw a diagram depicting steps of ELISA. Explain a) ELISA is a semiquantitative-assay, b) application of ELISA c) types of ELISA | 07 |
| OR | |
| (c) Justify the statement “Louis Pasteur is aptly known as Father of Microbiology | 07 |
| Q.3 | |
| (a) Draw a diagram to depict Holliday junction. How does it help in evolution? | 03 |
| (b) Microbial growth occurs is observed as sigmoidal curve. Describe each phase of the sigmoidal curve. During which phase the primary metabolites are formed? | 04 |
| (c) Describe PCR, its applications, advantages and disadvantages. Draw a diagram to depict PCR cycle and enlist the ingredients used for PCR | 07 |
| OR | |
| Q.3 | |
| (a) Enlist the significance of microorganisms in agricultural microbiology | 03 |
| (b) Write a descriptive note on IMViC Test. Write the IMViC reaction for any two microorganisms | 04 |
| (c) A butcher, asymptomatic carrier of Salmonella, minced meat without wearing gloves. As consequence, the meat was contaminated with 25 cells of Salmonella spp. and 32 cells of S. enterica. Taking into account that the generation time of Salmonella spp. is 30 minutes and its lag phase is 3 h, and that the specific growth rate constant of S. enterica using meat as substrate is 0.17 h! and its lag phase lasts 5 h, calculate the number of Salmonella cells that will be present in the meat 10 hours after being prepared. | 07 |
| Q.4 | |
| (a) Explain the formation of HFr and F’ strains formed during bacterial conjugation. Also write about the significance of “Merozygote” | 03 |
| (b) In a picnic, the paella was contaminated with 46 cells of Propionibacterium acnes. If P. acnes has a generation time of 90 minutes and a lag phase of 1.5 h, how many cells of this bacterium will be present in the paella after 10 h? | 04 |
| (c) Bacteria change from non-pathogenic strain to pathogenic strain. Explain the concept with experiment conducted by Griffith. Also suggest an application in reference to food industry. | 07 |
| OR | |
| Q.4 | |
| (a) Draw a diagram to depict parts of compound bright field microscope. | 03 |
| (b) Give example of microorganisms 1) acid fast bacteria 2) photoautotrophic bacteria 3) bacteria producing bacteriocin 4) fungi used for commercial production of penicillin | 04 |
| (c) Explain DNA replication. Why lagging strand is named as such? Enlist the name and functions of all enzymes and proteins involved in DNA replication. | 07 |
| Q.5 | |
| (a) Explain replica plating method for isolation of auxotroph. Explain the term bradytrophic bacteria. | 03 |
| (b) Draw labelled diagram to depict arrangement of flagella in bacteria | 04 |
| (c) Draw a diagram to depict transduction. What is lytic and lysogenic cycle? Explain the term specialized transduction. | 07 |
| OR | |
| Q.5 | |
| (a) A blue cheese sample was crushed in saline and serially diluted upto 10-7. The last dilution when spread plated resulted in 200 colonies. What should be interpreted by the analyst regarding the cell count in terms of log cfu/ml? Can you speculate the results in plates treated similarly for 10-6 and 10-8 dilutions | 03 |
| (b) Explain biosafety levels in terms of lethalness of pathogenicity and availability of cure. Name any 4 centers in India where biosafety level-4 can be handled. Give examples of ways/ organisms used for bioterrorism during ancient times/ currently | 04 |
(c) Give reason for the following (briefly; diagram not required)
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