GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER- V (New) EXAMINATION - WINTER 2019
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Subject Code: 2150401 Date: 25/11/2019
Subject Name: Advanced Molecular Biology - I
Time: 10:30 AM TO 01:00 PM Total Marks: 70
Instructions:
- Attempt all questions.
- Make suitable assumptions wherever necessary.
- Figures to the right indicate full marks.
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MARKS
Q.1 (a) Give functions of: DNA Ligase, Restriction Endonuclease, Linker sequence. 03
(b) Explain steps of gene cloning in brief. 04
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(c) Explain the technique of Southern Hybridization. 07
Q.2 (a) Compare sticky and blunt ends generated by RE. 03
(b) Explain structure and regulation of Fertility plasmid. 04
(c) Explain any one vector in detail along with its markers. 07
OR
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(c) Explain the possibilities for insertion of DNA insert into vector. 07
Q.3 (a) What are cloning and expression vectors? 03
(b) Explain the process of formation of Holliday junction. 04
(c) Explain principle and working of PCR. 07
OR
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Q.3 (a) Explain principle of electrophoresis. 03
(b) How is F prime generated in E.coli? 04
(c) Explain double strand break model in recombination. 07
Q.4 (a) Compare cDNA and Genomic library. 03
(b) What are the methods to induce artificial competence? 04
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(c) Explain process of transfer of DNA from donor to recipient during conjugation process. 07
OR
Q.4 (a) What are Hfr Strains? 03
(b) What is transduction? 04
(c) Explain the process of development of natural competence in Streptococcus. 07
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Q.5 (a) Give properties of good host. 03
(b) Explain concept of reverse genetics. 04
(c) Explain abortive transduction in detail. 07
OR
Q.5 (a) Compare DNA transfer in conjugation, transduction and transformation. 03
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(b) Narrate applications of gene therapy. 04
(c) Explain specialized transduction in detail. 07
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