Download GTU BE/B.Tech 2019 Winter 7th Sem New 2170401 Enzymes And Proteins Question Paper

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GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER- VII (New) EXAMINATION — WINTER 2019

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Subject Code: 2170401 Date: 23/11/2019
Subject Name: Enzymes and Proteins
Time: 10:30 AM TO 01:00 PM Total Marks: 70

Instructions:

1. Attempt all questions.

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2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

MARKS

Q.1 (a) Name any three substrates for enzyme immobilization. 03
(b) Explain collision theory of enzyme action. 04

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(c) Give the Classification of enzymes in detail. 07

Q.2 (a) Explain collision theory of enzyme catalysis. 03
(b) Explain the competitive inhibition of enzyme. 04
(c) What is enzyme immobilization? Discuss various physical techniques of enzyme immobilization. 07
OR

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(c) Discuss the concept of active site and energetics of enzyme substrate complex formation! 07

Q.3 (a) Comment on the diagnostic use of enzymes. 03
(b) Comment on the allosteric regulation of enzyme action. 04
(c) Give the derivation of Michelis-Menten equation and comment on the significance of it. 07
OR

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Q.3 (a) Write the advantages of enzyme immobilisation. 03
(b) Write the limitations of enzyme immobilisation. 04
(c) Explain industrial applications of Enzymes. 07

Q.4 (a) Diagnostic use of enzymes. 03
(b) What is enzyme specificity? 04

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(c) Explain General properties of protein-protein interfaces. 07
OR

Q.4 (b) Discuss features of structural relationship among homologous proteins. 04
(c) Discuss in detail about protein mis-folding diseases. 07

Q.5 (a) What is native state of protein structure 03

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(b) How new functions evolved in proteins. 04
(c) Explain thermodynamics of protein folding with example of free energy funnels. 07
OR
Q.5 (a) What is proteostasis? 03
(b) What are chaperons? 04

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(c) Discus effects of different protein denaturation agents on protein. 07

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