GTU BE 2019 Summer Question Papers || Gujarat Technological University
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Seat No.:
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Subject Code: 2131407
Enrolment No.
GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER-III (NEW) EXAMINATION — SUMMER 2019
Subject Name: Basic Food Microbiology
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Time: 02:30 PM TO 05:00 PM
Instructions:
- Attempt all questions.
- Make suitable assumptions wherever necessary.
- Figures to the right indicate full marks.
- Draw diagrams wherever necessary.
- Write scientific names as per the norms
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| S. | MARKS | |
|---|---|---|
| Q.1 | (a) Draw a flowchart to depict the scheme of five kingdom classification based on characteristics. | 03 |
| (b) Justify why Robert Koch is also known as Father of Modern bacteriology | 04 | |
| (c) Describe the difference between gram +ve and gram –ve bacteria. Give example of gram +ve rod, gram +ve cocci, gram –ve rod, gram –ve cocci | 07 | |
| Q.2 | (a) Write a short note on chemical and physical methods to control microorganisms | 03 |
| (b) Microbial growth occurs is observed as: sigmoidal curve. Describe each phase of the sigmoidal curve. During which phase the primary metabolites are formed? | 04 | |
| (c) Describe the difference between eukaryotic and prokaryotic cell | 07 | |
| OR | ||
| (c) Justify the statement “Louis Pasteur is aptly known as Father of Microbiology | 07 | |
| Q.3 | (a) An apple pie sample contained 10000 cells of S. aureus. And it was kept at room temperature for 4 hrs. What would be the generation time if the final no. of cells is 6.5 X 10^8. | 03 |
| (b) Explain Redi’s, Needham’s and Spallanzani’s experiments. Which scientist favored concept of biogenesis | 04 | |
| (c) Using a schematic diagram, describe the steps of western blotting technique. For which substance, northern blotting is used? | 07 | |
| OR | ||
| Q.3 | (a) Draw a diagram to depict procedure of gram staining | 03 |
| (b) Draw a flowchart to depict the health benefits rendered by probiotics. Give examples for the probiotics which render health benefit in large intestine and small intestine | 04 | |
| (c) Describe PCR, its applications, advantages and disadvantages. Draw a diagram to depict PCR cycle and enlist the ingredients used for PCR | 07 | |
| Date: 07/06/2019 | ||
| Total Marks: 70 | ||
| Q.4 | (a) Describe steps of bacterial DNA exchange by conjugation using a well labeled diagram. Also write about the significance of HFr and F’ | 03 |
| (b) Describe morphology of bacteria and conventions followed for nomenclature of microorganisms | 04 | |
| (c) Bacteria change from non-pathogenic strain to pathogenic strain. Explain the concept with experiment conducted by Griffith. Also suggest an application in reference to food industry. | 07 | |
| OR | ||
| Q.4 | (b) Draw a diagram to depict parts of compound bright field microscope. Why immersion oil is necessary while using 100X objective? | 03 |
| (c) Draw a diagram to depict Holliday junction. How does it helps in evolution? | 04 | |
| Q.5 | (a) Draw A diagram to depict lysogenic and lytic phases of transduction. Explain the concept of Specialized transduction. | 07 |
| (b) What is direct and indirect ELISA and list down applications. | 03 | |
| (c) Write a short note on contribution of Lady Mary Wortley Montagu in the field of immunology | 04 | |
| Q.5 | (b) Explain the concept of IMViC test used for differentiating enteric bacteria | 07 |
| OR | ||
| (b) If 220 colonies were obtained on a given plate which was prepared by pour plating with 7th dilution, determine the microbial count in terms of log cfu/ml | 03 | |
| (c) Explain the role of intrinsic and extrinsic factors which affect the microbial load in foods | 04 | |
| (c) Justify how moist heat is better option for sterilization than dry heat. Explain the terms antiseptic, aseptic, sanitization; sterilization. | 07 | |
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