Subject Code: 2160401
GUJARAT TECHNOLOGICAL UNIVERSITY
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BE - SEMESTER-VI (NEW) EXAMINATION - WINTER 2018Subject Name: Advanced Molecular Biology-II
Time: 02:30 PM TO 05:00 PM
Date: 16/11/2018
Total Marks: 70
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Instructions:
1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
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What is significance of RNA splicing? (03)
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Explain base substitution mutation with suitable illustration. (04)
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What are transposable elements? Explain its basic structure. (07)
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Define the terms reversion and suppression of mutation. (03)
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Write a short note on base analogue mutagens. (04)
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Explain positive regulation of lac operon (07)
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OR
Explain negative regulation of lac operon. (07)
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What is significance of gene mapping? (03)
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Explain regulation of replication at genomic level in eukaryotes (04)
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Explain mismatch repair mechanism in detail. (07)
OR
Explain primer walking. (03)
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Explain any one mechanism of regulation of metabolic pathway. (04)
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Explain intragenic suppression mechanism. (07)
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What are post transcriptional controls? (03)
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Explain attenuation mechanism. (04)
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Explain lifecycle of T4 phage. (07)
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OR
What is alternative and trans-splicing? (03)
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Write a note on splicing errors. (04)
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Explain splicing mechanism of nuclear mRNA. (07)
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What is lysogeny? Give example of any one virus which performs lysogeny. (04)
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What are integrons and retrotransposons? (07)
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Explain the technique of DNA fingerprinting. (03)
OR
Explain molecular basis of mutation. (04)
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Draw structure of retrovirus. (07)
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Explain short gun DNA sequencing approach. (07)
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