Download GTU BE/B.Tech 2018 Winter 6th Sem New 2160508 Biochemical Engineering Question Paper

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Subject Code:2160508

GUJARAT TECHNOLOGICAL UNIVERSITY

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BE - SEMESTER-VI (NEW) EXAMINATION - WINTER 2018

Subject Name:Biochemical Engineering

Time: 02:30 PM TO 05:00 PM

Date:30/11/2018

Total Marks: 70

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Instructions:

1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

Q.1

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1. Discuss any two techniques used to extract intracellular product via Cell Disruption. (03 MARKS)
2. Explain different methods for the determination of kLa (04 MARKS)
3. Discuss the material of construction used in the design of bioreactors with example (07 MARKS)

Q.2

1. Define Precipitation and Filtration techniques used in downstream processing. (03 MARKS)

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2. Discuss various developments that took place in history for biochemical engineering and its products. (04 MARKS)
3. Explain the methods for the maintenance of Aseptic condition and containment (07 MARKS)

OR

1. Explain the Lock and Key model with diagram for enzymatic reactions (07 MARKS)

Q.3

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1. List various types of valves used in biochemical process industry (03 MARKS)
2. Discuss the role of impellers used for agitation in fermentor in brief. (04 MARKS)
3. Discuss in detail the difference between batch culture and Fed-batch culture. (07 MARKS)

OR

1. Define Monod growth kinetics and its application (03 MARKS)

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2. Write a short note on OTR and OUR (04 MARKS)
3. Discuss in brief the variants of fermentation vessels (07 MARKS)

Q.4

1. Explain in brief what happens during Lag phase and exponential phases of microbial growth (03 MARKS)
2. Explain the importance of pH in fermentation or enzymatic process for product formation (04 MARKS)

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3. Write short note on antibiotics and its application (07 MARKS)

OR

1. Discuss the challenges faced during Scale-Up of fermentation process. (03 MARKS)
2. Define crystallization and state its application in biochemical industry (04 MARKS)
3. Discuss the methods to enhance aeration in a fermentor for an aerobic process. (07 MARKS)

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Q.5

1. Define Enzyme inhibition and role of cofactors (03 MARKS)
2. Write a brief note on various sterilization techniques of a fermentation process (04 MARKS)
3. Discuss various types of steam traps used in fermentation vessel (07 MARKS)

OR

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1. Discuss the importance of substrate concentration for any biochemical reaction (04 MARKS)
2. Discuss the importance of Fluid rheology in design of any fermentation process equipment (04 MARKS)
3. Discuss the basic functions of a fermenter for microbial or animal cell culture (07 MARKS)