First Year M.Pharm Degree Examination – May 2012
[Time: 3 Hours] [Max. Marks: 100]
MOLECULAR BIOLOGY
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PAPER IV
(RS 2 & RS 3)
Q.P. CODE: 9244
Your answers should be specific to the questions asked.
Draw neat labeled diagrams wherever necessary. Answer all questions
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LONG ESSAY (Answer any TWO) 2 X 20 = 40 Marks
- Discuss principle, process and isolation of gene cloning of vectors
- Define Polymerase chain reaction. Explain in detail about polymerase chain reaction technique and its applications. And a note on variants of PCR.
- a) Pharmacodynamics and immunogenicity of protein therapeutics
b) Explain the production of growth hormone by recombinant technology
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SHORT ESSAY (Answer any FIVE) 5 X 10 = 50 Marks
- Biosensors
- Diagnosis of genetic diseases
- Discuss DNA sequencing
- Write a note on enzymes used in molecular cloning
- Principle and applications of ligation chain
- Discuss briefly the genetic elements that control gene expression.
- Cell cycle
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SHORT NOTES 2 X 5 = 10 Marks
- Catalytic antibodies
- Chain reaction
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