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Rajiv Gandhi University of Health Sciences, Karnataka
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First Year M. Pharm Degree Examination – MAY 2016
Time: Three Hours
Max. Marks: 100 Marks
MEDICINAL PLANT CULTIVATION AND BIOTECHNOLOGY
(Revised scheme 4)
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Q.P. CODE: 9386
Your answers should be specific to the questions asked.
Draw neat labeled diagrams wherever necessary
LONG ESSAYS (Answer any Two)
2 x 20 = 40 Marks
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- Explain the technology for commercial scale cultivation of Ashwagandha and Lemon grass.
- Explain the strategies including culture condition, use of precursors and elicitors, immobilization and hairy root culture for enhancement of secondary metabolites in plant tissue culture.
- What are transgenic plants? How do you prepare them? What are the approaches for production of Insect, fungus, virus and herbicide resistance plants?
SHORT ESSAYS (Answer any FIVE)
5 x 10 = 50 Marks
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- Explain the role of mutation and polyploidy in improving the quality of medicinal plants with examples.
- What are RFLP and RAPD? Explain their applications in authentication and detection of adulterants.
- Explain the isolation and purification of enzymes from different sources.
- Write notes on: plant chromosome analysis and bioinformatics.
- Give details on current status of national and international trade in medicinal plants.
- In-vitro conservation and cryopreservation are useful in germplasm conservation. Explain the significance of the statement. Give details of the different techniques.
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SHORT NOTES
2 x 5 = 10 Marks
- Explain the use of bioreactors in plant cell culture with examples.
- Explain the production and uses of Papain.
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