Download PTU B.Tech 2020 March Biotechnology 5th Sem BTBT 503 Genetic Engineering Question Paper

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Roll No. EEEEEEEE [ ] Total No. of Pages : 02

Total No. of Questions : 09

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B.Tech.(BT) (2012 to 2017) (Sem.-5)

GENETIC ENGINEERING

Subject Code : BTBT-503

M.Code : 70504

Time : 3 Hrs. Max. Marks : 60

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INSTRUCTION TO CANDIDATES :

1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students have to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students have to attempt any TWO questions.

SECTION-A

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1. Answer briefly :
   1. Differentiate between BamHI and BamHI Methyltransferase.
   2. Distinguish between E. coli DNA Polymerase I and Klenow fragment.
   3. Differentiate between plasmid-and cosmid vector.
   4. “E. coli is a preferred host in molecular cloning”. Justify the statement.
   5. Elucidate the principle of Southern blot analysis.

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   6. State briefly the biological importance of DNA-protein interactions.
   7. What are the ingredients required to set up a PCR?
   8. What is DNA microarray technique?
   9. How do you demonstrate the expression of a recombinant protein in E. coli?
   10. Write a brief note on in vitro transcription.

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SECTION-B

2. State the attributes and applications of Kinases and Ligases with examples.
3. How do you construct a mouse liver cDNA library?

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4. What do mean by gene-specific probes? How do you radiolabel them?
5. Write briefly any four applications of PCR.
6. Write the principle and applications of site-directed mutagenesis.

SECTION-C

7. Illustrate the genetic features of the yeast and mammalian expression vectors.

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8. Write in detail the principle and steps of oligonucleotide synthesis.
9. Write precise notes on the following :
   YAC vector, Western blot analysis.

NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any page of Answer Sheet will lead to UMC against the Student.

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