Download PTU B.Tech 2020 March Biotechnology 7th and 8th Sem BTBT 702 Downstream Processing Question Paper

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Roll No. [ ] Total No. of Pages : 02

Total No. of Questions : 09

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B.Tech.(BT) (2012 to 2017) (Sem.-7)

DOWNSTREAM PROCESSING

Subject Code : BTBT-702

M.Code : 71844

Time : 3 Hrs. Max. Marks : 60

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INSTRUCTION TO CANDIDATES :

1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students have to attempt ANY FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students have to attempt ANY TWO questions.

SECTION-A

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1. Write briefly :
   1. Selection criteria for downstream processing of a fermentation product.
   2. Sedimentation coefficient of biological macromolecules.
   3. Define ‘Selectivity Factor’.
   4. Enlist various methods used for precipitation of proteins.
   5. Name any two flocculating agents.

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   6. Salting-in and salting-out phenomenon for protein purification.
   7. Isoelectric focusing.
   8. Ultrafiltration.
   9. Define the term ‘Partition Coefficient’.
   10. Principle of capillary electrophoresis.

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SECTION-B

2. Describe the process design criteria for various classes of bio-products.
3. Explain the effect of pressure drop on cake resistance in a batch filtration. What is the effect of compressible cake formation on filtration process?
4. Explain the principle of centrifugal separation. Mention the important features of tubular bowl centrifuge along with a neat sketch.

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5. What are adsorption isotherms? Explain the adsorption in fixed beds.
6. A colloidal suspension of clay became clear upon being allowed to stand undisturbed for 5 minutes in ethanol at 20°C. The height of the suspension in the vessel was 45 cm and the specific gravity of montmorillonite is known to be 1.92 g/cm³. The viscosity of ethanol at 20°C was calculated to be 1.2 centipoises. Estimate the average diameter of clay particles.

SECTION-C

7. Define “crystallization”. Explain various phases of a crystallization process. Derive the relationship between size and population density of crystals formed during continuous crystallization.
8. 1. Write a complete note on working principle, instrumentation and characteristics of a chromatographic techniques: exclusive for protein separation. Also mention some matrices used for large-scale chromatography.

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   2. Define ion-exchange and Principle of ion-exchange chromatography. Mention the applications of ion-exchangers.
9. Comment on following (also provide the necessary sketch) :
   1. Two phase extraction process
   2. Dialysis

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NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any page of Answer Sheet will lead to UMC against the Student.

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