Hemwati Nandan Bahuguna Uttarakhand Medical Education University
Administrative Block, Govt. Doon Medical College Campus, Dehrakhas, Patel Nagar, Dehradun - 248001
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Phone: 0135 - 2723323 Fax: 0135 - 2723323Website: www.hnbumu.ac.in E-mail: firstranker.com
Ph.D. ENTRANCE EXAMINATION - 2020
Subject : Microbiology Duration: 01:30 Hrs.
Date : 06.01.2020 Place : Dehradun
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Max Marks: 60Student Name: Invigilator Name:
Signature: Signature:
- The dye eosinate of methylene blue belongs to which group?
- Acidic dye
- Basic dye
- Neutral dye
- Oxazine dye
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- What is the correct order of staining reagents in Gram-Staining?
- Crystal violet, alcohol, iodine solution, safranin
- Crystal violet, iodine solution, alcohol, safranin
- Crystal violet, safranin, alcohol, iodine solution
- Iodine solution, crystal violet, alcohol, safranin
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- Which bacteria appears purple-violet colour after staining?
- Gram-positive
- Gram-negative
- Both Gram-positive and Gram-negative
- Neither Gram-positive nor Gram-negative
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- Which of the following are true for Gram-negative bacteria?
- upon alcohol treatment, the permeability of the cell wall increases
- crystal violet-iodine (CV-I) complex is extracted
- pore size decreases and the CV-I complex cannot be extracted
- alcohol treatment increases the permeability of the cell wall and the CV-I complex can be extracted
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- Gram-positive bacteria are usually more susceptible to?
- streptomycin
- tetracyclin
- penicillin
- ampicillin
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- Which of the staining technique helps in demonstrating spore structure in bacteria as well as free spores?
- Acid-fast stain
- Endospore stain
- Capsule stain
- Flagella stain
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- In Gram-staining, iodine is used as a
- fixative
- mordant
- solublizer
- stain
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- Which of the following is the example of Gram-negative bacteria?
- Lactobacillus
- Eschericia coli
- Staphylococcus aureus
- Bacillus subtilis
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- Which of the following is used in electron microscope?
- electron beams
- magnetic fields
- light waves
- electron beams and magnetic fields
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- Electron Microscope can give a magnification up to
- 400,000X
- 100,000X
- 15000X
- 100X
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- Which group of bacteria among the following have the largest area of contact?
- Palisades
- Trichomes
- Streptobacilli
- Diplobacilli
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- Which of the following are functions of stalk?
- nutrient absorption
- motility
- attachment of the cells to surfaces
- human infection
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- Salmonella typhi has which type of flagella arrangement?
- Amphitrichous
- Peritrichous
- Monotrichous
- Lophotrichous
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- The L Ring in a Gram-Negative bacterium flagella is associated with
- Peptidogycan
- Outer Membrane
- Cytoplasmic Membrane
- Cell Membrane
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- F pilus has a major role as
- motility of the cell
- port of entry of genetic material during mating
- attachment to host cell
- human infection
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- Prosthecae helps in
- motility
- nutrient absorption and attachment to surfaces
- human infection
- protection from environment
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- When a bacteria swim towards a chemical, it is termed as
- positive chemotaxis
- phototaxis
- negative chemotaxis
- magnetotaxis
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- Which type of force drives the flagellar motion?
- Protonmotive force
- ATP driven
- Protonmotive and ATP driven
- No protonmotive nor ATP driven
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- Peptidoglycan is made up of
- N-acetylglucosamine
- N-acetylmuramic acid
- N-acetylglucosamine, N-acetylmuramic acid
- N-acetylglucosamine,N-acetylmuramicacid,amino acids
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- Teichoic acid present in Gram-positive bacteria can bind to which ion?
- Feions
- Phosphorus ions
- Mgions
- Sulphur ions
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- Cord factor is a
- protein
- teichoic acid derivative
- mycolic acid derivative
- carbohydrate
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- The outer membarane of the Gram-negative cell wall is anchored to the underlying peptidoglycan by means of which of the following?
- Braun’s Lipoprotein
- Phospholipids
- Proteins
- Lipopolysaccharide
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- NAG and NAM of peptidoglycan layer is linked by
- beta-(1,4) glycosidic linkage
- alpha-(1,4) glycosidic linkage
- alpha-(1,6) glycosidic linkage
- beta-(1,6) glycosidic linkage
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- Which of the following methods can be utilized for removing peripheral proteins of the cytoplasmic membrane?
- treatment by detergents
- osmotic shock
- heat application
- destruction of the membrane
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- Which of the following are functions of water in the culture medium?
- nutrients must be in aqueous solution
- cofactor of enzymes
- provides resistance to sudden transient temperature changes
- it is a chemical reactant, nutrients must also be present in aqueous solution and provides resistance to sudden temperature changes.
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- The isolation of gonorrhea-causing organism, Neisseria gonorrhoeae by the use of certain antibiotics in media is an example of which of the following?
- Selective media
- Differential media
- Enriched media
- Assay media
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- Nutrient broth, a liquid media contains beef extract and peptone respectively in how much amounts?
- 0.2%, 0.4%
- 0.1%, 0.6%
- 0.3%, 0.5%
- 0.7%, 0.3%
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- Which of the following instrument is used for sterilizing the media after it has been prepared?
- Autoclave
- Laminar Air Flow Chamber
- Inoculum Needle
- Incubator
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- Which of the following is a Complex media for fungal growth?
- Nutrient broth
- Luria-Bertani media
- Potato Dextrose Agar(PDA) media
- Mac Conkey Agar media
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- Which of the following bacteria requires nicotinic acid as a growth factor in their media?
- Proteus vulgaris
- Nitrosomonas sp.
- E. coli
- Leuconostocmesenteroides
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- Which of the following factors are responsible for the stability of thermophiles at high temperatures?
- increased leakage of cell components
- presence of large no. of polar amino acids and alpha-helix protein
- thermal stability of ribosomes
- presence of Inositol diphosphate and thermal stability of ribosomes
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- The bacterium Staphylococcus aureus is which type of bacteria?
- Psychrophile
- Mesophile
- Thermophile
- Mesophile and psychrophile
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- The Reactive Oxygen Species(ROS) poduced by some bacteria are degraded by which of the following enzymes?
- Peroxidase
- Lyase
- Catalase
- Superoxide dismutase, Catalase and Peroxidase
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- HIV is transmitted by all of the following routes except:
- Saliva
- Needle prick injury
- Blood transmission
- Sexual intercourse
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- Arise in the anti-HBc immunoglobulin in a patient indicates
- Acute infection
- Carrier state
- Prodromal phase
- Convalescence
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- All of the following infections may be transmitted be dental instruments except:
- HIV
- Hepatitis C
- Hepatitis B
- Hepatitis E
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- The culture media used for cultivation of HSV is
- Chocolate agar
- Robertoson's cooked-meat broth
- Chorioallantoic membrane
- Sabourad's agar
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- The causative organism of syphilis is:
- Borreliaburgolorferi
- Chalamydia trachomatis
- Leptospirainterrogans
- Treponemapallidum
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- On a stained slide, clostridium tetani has the appearance of a:
- Bunch of a
- Chain of beads
- Drum stick
- Safely pin
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- An oxidase-positive, gram-negative rod which produces a bluish-green pigment has been grown in culture from a swab obtained from an infected burn wound. This organism is susceptible to gentamicin, ticarcillin and tobramicin, but resistant to all the other antibiotics which of the following organisms is it likely to be?
- Escherichia coli
- Klebsiellapnemoniae
- Proteus miribilis
- Pseudomonas aeruginosa
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- In initial stages of tyhoid, salmonella can be detected in:
- Feacus
- Urine
- Blood
- All of the above
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- Signs of tyhoid fever is/are
- Haemorrhage
- perforation
- Osteomyelitis
- All of the above
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- All of the following are true of streptococcus except:
- It's group of lactic acid production bacteria
- Shows adherence to dental enamel
- Most common in humans
- Most common is animals
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- Electron microscopic examination of the bacterial flora of necrotising ulcerative gingivitis indicates the presence of microorganisms within non-necrotic tissues in advance of other bacteria. The organisms involved are:
- Cocci
- Spirochetes
- Bacteriophages
- Filamentous rods
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- The following are applicable to bacterial genoms except:
- Itis composed of DNA
- It doe not contain histones
- Itis circular
- Its DNA has both introns and extrons
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- The commonest method of detection of diptheria carriers is:
- Schick test
- Dick test
- Casoni's test
- Charles' test
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- Each of the following can cause food poisoning except:
- Cl. Difficile
- Staphylococcus
- CL. Welchii
- CL botulinum
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- Germ tubes are formed mainly by:
- Candida albicans
- Candida stellatoidea
- Candida tropicalis
- Candida pseudotropicalls
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- Fungus capable to forming fungal ball is:
- Penicillium
- Aspergillus
- Mucor
- Rhizopus
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- A sporangium contains:
- Spherules
- Porangiospores
- Chlamydospores
- Oidia
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- Transmission of all of the following intestinal parasites takes place by the feco-oral route except:
- Giardia lambia
- Ascaris lumbriocoides
- Strongyloides
- Entamoeba
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- Hydatid cyst is:
- Parasitic in nature
- Fungal
- Congenital
- Viral
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- Investigation of choice for invasive amoebiasis is:
- Indirect hemagglutination
- ELISA
- Counter immune electroporesis
- Microscopy
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- L.D bodies are seen in:
- Kalahaza
- Toxoplasmosis
- Malaria
- Sleeping sickness
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- Parasitic inflammation would show predominantly
- Lymphocytes
- Neutrophilis
- Eosinophils
- Basophils
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- Amoeb all are seen in a cysts of E. histolytica except:
- Glycogen mass
- Chromatid bars
- Eccentric nucleus
- Refractile nucleus
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- Example of immune complex hyper-sensitivity reaction is:
- Atopic allergy
- Serum sickness
- Transfusion reaction
- Contact dermatitis
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- Cysticercosis celluosae is caused by:
- T.solium.
- Echinococcus granulosus
- T. Saginata
- H.nana
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- The predominant immunoglobulin in saliva is:
- IgA
- IgD
- IgE
- IgG
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- Steam under pressure in used in ___ sterilization
- Pasteurization
- Autoclave
- Kochs sterilizer
- hot air oven
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This download link is referred from the post: DNB 2012 June Previous Question Papers-(Diplomate of National Board) Under NBE
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