Download Hemwati Nandan Bahuguna Uttarakhand Medical Education University (HNBUMU) Medical Entrance Exam 2020 Jan Microbiology Question Paper With Answer Previous Question Paper With Solution
Hemwati Nandan Bahuguna Uttarakhand Medical
Education University
Administrative?Block,?Govt.?Doon?Medical?College?Campus,?Dehrakhas,??
Patel?Nagar,?Dehradun???248001?
Phone:?0135???2723323?Fax:? 0135???2723323?
Website: www.hnbumu.ac.in? E?mail:?info.hnbumu@gmail.com
Ph.D. ENTRANCE EXAMINATION - 2020
Subject? :??Microbiology? ? ? ??????????? Duration:?01:30?Hrs.?
Date?? :??06.01.2020? ? ??????????? Place?:?Dehradun?
Max?Marks:?60?? ?
?
Student?Name:??????Invigilator?Name:??
?
Signature:???????Signature:?
FirstRanker.com - FirstRanker's Choice
Hemwati Nandan Bahuguna Uttarakhand Medical
Education University
Administrative?Block,?Govt.?Doon?Medical?College?Campus,?Dehrakhas,??
Patel?Nagar,?Dehradun???248001?
Phone:?0135???2723323?Fax:? 0135???2723323?
Website: www.hnbumu.ac.in? E?mail:?info.hnbumu@gmail.com
Ph.D. ENTRANCE EXAMINATION - 2020
Subject? :??Microbiology? ? ? ??????????? Duration:?01:30?Hrs.?
Date?? :??06.01.2020? ? ??????????? Place?:?Dehradun?
Max?Marks:?60?? ?
?
Student?Name:??????Invigilator?Name:??
?
Signature:???????Signature:?
1. The dye eosinate of methylene blue belongs to which group?
a)?Acidic?dye?
b)?Basic?dye?
c)?Neutral?dye?
d)?Oxazine?dye?
?
2.?What is the correct order of staining reagents in Gram-Staining?
a)?Crystal?violet,?alcohol,?iodine?solution,?safranin?
b)?Crystal?violet,?iodine?solution,?alcohol,?safranin?
c)?Crystal?violet,?safranin,?alcohol,?iodine?solution?
d)?Iodine?solution,?crystal?violet,?alcohol,?safranin?
?
3.?Which bacteria appears purple-violet colour after staining?
a)?Gram?positive?
b)?Gram?negative?
c)?Both?Gram?positive?and?Gram?negative?
d)?Neither?Gram?positive?nor?Gram?negative?
?
4.?Which of the following are true for Gram-negative bacteria?
a)?upon?alcohol?treatment,?the?permeability?of?the?cell?wall?increases?
b)?crystal?violet?iodine?(CV?I)?complex?is?extracted?
c)?pore?size?decreases?and?the?CV?I?complex?cannot?be?extracted?
d)?alcohol?treatment?increases?the?permeability?of?the?cell?wall?and?the?CV?I?complex?can?be?
extracted?
5.?Gram-positive bacteria are usually more susceptible to?
a)?streptomycin?
b)?tetracyclin?
c)?penicillin?
d)?ampicillin?
6.?Which of the staining technique helps in demonstrating spore structure in bacteria as
well as free spores?
a)?Acid?fast?stain?
b)?Endospore?stain?
c)?Capsule?stain?
d)?Flagella?stain?
?
FirstRanker.com - FirstRanker's Choice
Hemwati Nandan Bahuguna Uttarakhand Medical
Education University
Administrative?Block,?Govt.?Doon?Medical?College?Campus,?Dehrakhas,??
Patel?Nagar,?Dehradun???248001?
Phone:?0135???2723323?Fax:? 0135???2723323?
Website: www.hnbumu.ac.in? E?mail:?info.hnbumu@gmail.com
Ph.D. ENTRANCE EXAMINATION - 2020
Subject? :??Microbiology? ? ? ??????????? Duration:?01:30?Hrs.?
Date?? :??06.01.2020? ? ??????????? Place?:?Dehradun?
Max?Marks:?60?? ?
?
Student?Name:??????Invigilator?Name:??
?
Signature:???????Signature:?
1. The dye eosinate of methylene blue belongs to which group?
a)?Acidic?dye?
b)?Basic?dye?
c)?Neutral?dye?
d)?Oxazine?dye?
?
2.?What is the correct order of staining reagents in Gram-Staining?
a)?Crystal?violet,?alcohol,?iodine?solution,?safranin?
b)?Crystal?violet,?iodine?solution,?alcohol,?safranin?
c)?Crystal?violet,?safranin,?alcohol,?iodine?solution?
d)?Iodine?solution,?crystal?violet,?alcohol,?safranin?
?
3.?Which bacteria appears purple-violet colour after staining?
a)?Gram?positive?
b)?Gram?negative?
c)?Both?Gram?positive?and?Gram?negative?
d)?Neither?Gram?positive?nor?Gram?negative?
?
4.?Which of the following are true for Gram-negative bacteria?
a)?upon?alcohol?treatment,?the?permeability?of?the?cell?wall?increases?
b)?crystal?violet?iodine?(CV?I)?complex?is?extracted?
c)?pore?size?decreases?and?the?CV?I?complex?cannot?be?extracted?
d)?alcohol?treatment?increases?the?permeability?of?the?cell?wall?and?the?CV?I?complex?can?be?
extracted?
5.?Gram-positive bacteria are usually more susceptible to?
a)?streptomycin?
b)?tetracyclin?
c)?penicillin?
d)?ampicillin?
6.?Which of the staining technique helps in demonstrating spore structure in bacteria as
well as free spores?
a)?Acid?fast?stain?
b)?Endospore?stain?
c)?Capsule?stain?
d)?Flagella?stain?
?
7.?In Gram-staining, iodine is used as a______________
a)?fixative?
b)?mordant?
c)?solublizer?
d)?stain?
8.?Which of the following is the example of Gram-negative bacteria?
a)?Lactobacillus?
b)?Eschericia?coli?
c)?Staphylococcus?aureus?
d)?Bacillus?subtilis?
9.?Which of the following is used in electron microscope?
a)?electron?beams?
b)?magnetic?fields?
c)?light?waves?
d)?electron?beams?and?magnetic?fields?
10.?Electron Microscope can give a magnification up to ___________
a)?400,000X?
b)?100,000X?
c)?15000X?
d)?100X?
11.?Which group of bacteria among the following have the largest area of contact?
a)?Palisades?
b)?Trichomes?
c)?Streptobacilli?
d)?Diplobacilli?
12.?Which of the following are functions of stalk?
a)?nutrient?absorption?
b)?motility?
c)?attachment?of?the?cells?to?surfaces?
d)?human?infection?
13.?Salmonella typhi has which type of flagella arrangement?
a)?Amphitrichous?
b)?Peritrichous?
c)?Monotrichous?
d)?Lophotrichous?
?
FirstRanker.com - FirstRanker's Choice
Hemwati Nandan Bahuguna Uttarakhand Medical
Education University
Administrative?Block,?Govt.?Doon?Medical?College?Campus,?Dehrakhas,??
Patel?Nagar,?Dehradun???248001?
Phone:?0135???2723323?Fax:? 0135???2723323?
Website: www.hnbumu.ac.in? E?mail:?info.hnbumu@gmail.com
Ph.D. ENTRANCE EXAMINATION - 2020
Subject? :??Microbiology? ? ? ??????????? Duration:?01:30?Hrs.?
Date?? :??06.01.2020? ? ??????????? Place?:?Dehradun?
Max?Marks:?60?? ?
?
Student?Name:??????Invigilator?Name:??
?
Signature:???????Signature:?
1. The dye eosinate of methylene blue belongs to which group?
a)?Acidic?dye?
b)?Basic?dye?
c)?Neutral?dye?
d)?Oxazine?dye?
?
2.?What is the correct order of staining reagents in Gram-Staining?
a)?Crystal?violet,?alcohol,?iodine?solution,?safranin?
b)?Crystal?violet,?iodine?solution,?alcohol,?safranin?
c)?Crystal?violet,?safranin,?alcohol,?iodine?solution?
d)?Iodine?solution,?crystal?violet,?alcohol,?safranin?
?
3.?Which bacteria appears purple-violet colour after staining?
a)?Gram?positive?
b)?Gram?negative?
c)?Both?Gram?positive?and?Gram?negative?
d)?Neither?Gram?positive?nor?Gram?negative?
?
4.?Which of the following are true for Gram-negative bacteria?
a)?upon?alcohol?treatment,?the?permeability?of?the?cell?wall?increases?
b)?crystal?violet?iodine?(CV?I)?complex?is?extracted?
c)?pore?size?decreases?and?the?CV?I?complex?cannot?be?extracted?
d)?alcohol?treatment?increases?the?permeability?of?the?cell?wall?and?the?CV?I?complex?can?be?
extracted?
5.?Gram-positive bacteria are usually more susceptible to?
a)?streptomycin?
b)?tetracyclin?
c)?penicillin?
d)?ampicillin?
6.?Which of the staining technique helps in demonstrating spore structure in bacteria as
well as free spores?
a)?Acid?fast?stain?
b)?Endospore?stain?
c)?Capsule?stain?
d)?Flagella?stain?
?
7.?In Gram-staining, iodine is used as a______________
a)?fixative?
b)?mordant?
c)?solublizer?
d)?stain?
8.?Which of the following is the example of Gram-negative bacteria?
a)?Lactobacillus?
b)?Eschericia?coli?
c)?Staphylococcus?aureus?
d)?Bacillus?subtilis?
9.?Which of the following is used in electron microscope?
a)?electron?beams?
b)?magnetic?fields?
c)?light?waves?
d)?electron?beams?and?magnetic?fields?
10.?Electron Microscope can give a magnification up to ___________
a)?400,000X?
b)?100,000X?
c)?15000X?
d)?100X?
11.?Which group of bacteria among the following have the largest area of contact?
a)?Palisades?
b)?Trichomes?
c)?Streptobacilli?
d)?Diplobacilli?
12.?Which of the following are functions of stalk?
a)?nutrient?absorption?
b)?motility?
c)?attachment?of?the?cells?to?surfaces?
d)?human?infection?
13.?Salmonella typhi has which type of flagella arrangement?
a)?Amphitrichous?
b)?Peritrichous?
c)?Monotrichous?
d)?Lophotrichous?
?
?
14.?The L Ring in a Gram-Negative bacterium flagella is associated with_________
a)?Peptidogycan?
b)?Outer?Membrane?
c)?Cytoplasmic?Membrane?
d)?Cell?Membrane?
?
15.?F pilus has a major role as___________
a)?motility?of?the?cell?
b)?port?of?entry?of?genetic?material?during?mating?
c)?attachment?to?host?cell?
d)?human?infection?
?
16. Prosthecae helps in __________
a)?motility?
b)?nutrient?absorption?and?attachment?to?surfaces?
c)?human?infection?
d)?protection?from?environment?
?
17. When a bacteria swim towards a chemical, it is termed as__________?
a)?positive?chemotaxis?
b)?phototaxis?
c)?negative?chemotaxis?
d)?magnetotaxis?
?
18.?Which type of force drives the flagellar motion?
a)?Protonmotive?force?
b)?ATP?driven?
c)?Protonmotive?and?ATP?driven?
d)?No?protonmotive?nor?ATP?driven?
?
19.?Peptidoglycan is made up of __________?
a)?N?acetylglucosamine?
b)?N?acetylmuramic?acid?
c)?N?acetylglucosamine,?N?acetylmuramic?acid?
d)?N?acetylglucosamine,N?acetylmuramicacid,amino?acids?
?
?
20.?Teichoic acid present in Gram-positive bacteria can bind to which ion?
a)?Fe?ions?
FirstRanker.com - FirstRanker's Choice
Hemwati Nandan Bahuguna Uttarakhand Medical
Education University
Administrative?Block,?Govt.?Doon?Medical?College?Campus,?Dehrakhas,??
Patel?Nagar,?Dehradun???248001?
Phone:?0135???2723323?Fax:? 0135???2723323?
Website: www.hnbumu.ac.in? E?mail:?info.hnbumu@gmail.com
Ph.D. ENTRANCE EXAMINATION - 2020
Subject? :??Microbiology? ? ? ??????????? Duration:?01:30?Hrs.?
Date?? :??06.01.2020? ? ??????????? Place?:?Dehradun?
Max?Marks:?60?? ?
?
Student?Name:??????Invigilator?Name:??
?
Signature:???????Signature:?
1. The dye eosinate of methylene blue belongs to which group?
a)?Acidic?dye?
b)?Basic?dye?
c)?Neutral?dye?
d)?Oxazine?dye?
?
2.?What is the correct order of staining reagents in Gram-Staining?
a)?Crystal?violet,?alcohol,?iodine?solution,?safranin?
b)?Crystal?violet,?iodine?solution,?alcohol,?safranin?
c)?Crystal?violet,?safranin,?alcohol,?iodine?solution?
d)?Iodine?solution,?crystal?violet,?alcohol,?safranin?
?
3.?Which bacteria appears purple-violet colour after staining?
a)?Gram?positive?
b)?Gram?negative?
c)?Both?Gram?positive?and?Gram?negative?
d)?Neither?Gram?positive?nor?Gram?negative?
?
4.?Which of the following are true for Gram-negative bacteria?
a)?upon?alcohol?treatment,?the?permeability?of?the?cell?wall?increases?
b)?crystal?violet?iodine?(CV?I)?complex?is?extracted?
c)?pore?size?decreases?and?the?CV?I?complex?cannot?be?extracted?
d)?alcohol?treatment?increases?the?permeability?of?the?cell?wall?and?the?CV?I?complex?can?be?
extracted?
5.?Gram-positive bacteria are usually more susceptible to?
a)?streptomycin?
b)?tetracyclin?
c)?penicillin?
d)?ampicillin?
6.?Which of the staining technique helps in demonstrating spore structure in bacteria as
well as free spores?
a)?Acid?fast?stain?
b)?Endospore?stain?
c)?Capsule?stain?
d)?Flagella?stain?
?
7.?In Gram-staining, iodine is used as a______________
a)?fixative?
b)?mordant?
c)?solublizer?
d)?stain?
8.?Which of the following is the example of Gram-negative bacteria?
a)?Lactobacillus?
b)?Eschericia?coli?
c)?Staphylococcus?aureus?
d)?Bacillus?subtilis?
9.?Which of the following is used in electron microscope?
a)?electron?beams?
b)?magnetic?fields?
c)?light?waves?
d)?electron?beams?and?magnetic?fields?
10.?Electron Microscope can give a magnification up to ___________
a)?400,000X?
b)?100,000X?
c)?15000X?
d)?100X?
11.?Which group of bacteria among the following have the largest area of contact?
a)?Palisades?
b)?Trichomes?
c)?Streptobacilli?
d)?Diplobacilli?
12.?Which of the following are functions of stalk?
a)?nutrient?absorption?
b)?motility?
c)?attachment?of?the?cells?to?surfaces?
d)?human?infection?
13.?Salmonella typhi has which type of flagella arrangement?
a)?Amphitrichous?
b)?Peritrichous?
c)?Monotrichous?
d)?Lophotrichous?
?
?
14.?The L Ring in a Gram-Negative bacterium flagella is associated with_________
a)?Peptidogycan?
b)?Outer?Membrane?
c)?Cytoplasmic?Membrane?
d)?Cell?Membrane?
?
15.?F pilus has a major role as___________
a)?motility?of?the?cell?
b)?port?of?entry?of?genetic?material?during?mating?
c)?attachment?to?host?cell?
d)?human?infection?
?
16. Prosthecae helps in __________
a)?motility?
b)?nutrient?absorption?and?attachment?to?surfaces?
c)?human?infection?
d)?protection?from?environment?
?
17. When a bacteria swim towards a chemical, it is termed as__________?
a)?positive?chemotaxis?
b)?phototaxis?
c)?negative?chemotaxis?
d)?magnetotaxis?
?
18.?Which type of force drives the flagellar motion?
a)?Protonmotive?force?
b)?ATP?driven?
c)?Protonmotive?and?ATP?driven?
d)?No?protonmotive?nor?ATP?driven?
?
19.?Peptidoglycan is made up of __________?
a)?N?acetylglucosamine?
b)?N?acetylmuramic?acid?
c)?N?acetylglucosamine,?N?acetylmuramic?acid?
d)?N?acetylglucosamine,N?acetylmuramicacid,amino?acids?
?
?
20.?Teichoic acid present in Gram-positive bacteria can bind to which ion?
a)?Fe?ions?
b)?Phosphorus?ions?
c)?Mg?ions?
d)?Sulphur?ions?
?
21?Cord factor is a ___________?
a)?protein?
b)?teichoic?acid?derivative?
c)?mycolic?acid?derivative?
d)?carbohydrate?
?
22.?The outer membarane of the Gram-negative cell wall is anchored to the underlying
peptidoglycan by means of which of the following?
a)?Braun?s?Lipoprotein?
b)?Phospholipids?
c)?Proteins?
d)?Lipopolysaccharide?
?
23.?NAG and NAM of peptidoglycan layer is linked by _________?
a)?beta?(1,4)?glycosidic?linkage?
b)?alpha?(1,4)?glycosidic?linkage?
c)?alpha?(1,6)?glycosidic?linkage?
d)?beta?(1,6)?glycosidic?linkage?
?
24.?Which of the following methods can be utilized for removing peripheral proteins of
the cytoplasmic membrane??
a)?treatment?by?detergents?
b)?osmotic?shock?
c)?heat?application?
d)?destruction?of?the?membrane?
?
25.?Which of the following are functions of water in the culture medium?
a)?nutrients?must?be?in?aqueous?solution?
b)?cofactor?of?enzymes?
c)?provides?resistance?to?sudden?transient?temperature?changes?
d)?it?is?a?chemical?reactant,?nutrients?must?also?be?present?in?aqueous?solution?and?provides?
resistance?to?sudden?temperature?changes.?
?
26.?The isolation of gonorrhea-causing organism, Neisseria gonorrhoeae by the use of
certain antibiotics in media is an example of which of the following?
a)?Selective?media?
b)?Differential?media?
c)?Enriched?media?
d)?Assay?media?
?
?
FirstRanker.com - FirstRanker's Choice
Hemwati Nandan Bahuguna Uttarakhand Medical
Education University
Administrative?Block,?Govt.?Doon?Medical?College?Campus,?Dehrakhas,??
Patel?Nagar,?Dehradun???248001?
Phone:?0135???2723323?Fax:? 0135???2723323?
Website: www.hnbumu.ac.in? E?mail:?info.hnbumu@gmail.com
Ph.D. ENTRANCE EXAMINATION - 2020
Subject? :??Microbiology? ? ? ??????????? Duration:?01:30?Hrs.?
Date?? :??06.01.2020? ? ??????????? Place?:?Dehradun?
Max?Marks:?60?? ?
?
Student?Name:??????Invigilator?Name:??
?
Signature:???????Signature:?
1. The dye eosinate of methylene blue belongs to which group?
a)?Acidic?dye?
b)?Basic?dye?
c)?Neutral?dye?
d)?Oxazine?dye?
?
2.?What is the correct order of staining reagents in Gram-Staining?
a)?Crystal?violet,?alcohol,?iodine?solution,?safranin?
b)?Crystal?violet,?iodine?solution,?alcohol,?safranin?
c)?Crystal?violet,?safranin,?alcohol,?iodine?solution?
d)?Iodine?solution,?crystal?violet,?alcohol,?safranin?
?
3.?Which bacteria appears purple-violet colour after staining?
a)?Gram?positive?
b)?Gram?negative?
c)?Both?Gram?positive?and?Gram?negative?
d)?Neither?Gram?positive?nor?Gram?negative?
?
4.?Which of the following are true for Gram-negative bacteria?
a)?upon?alcohol?treatment,?the?permeability?of?the?cell?wall?increases?
b)?crystal?violet?iodine?(CV?I)?complex?is?extracted?
c)?pore?size?decreases?and?the?CV?I?complex?cannot?be?extracted?
d)?alcohol?treatment?increases?the?permeability?of?the?cell?wall?and?the?CV?I?complex?can?be?
extracted?
5.?Gram-positive bacteria are usually more susceptible to?
a)?streptomycin?
b)?tetracyclin?
c)?penicillin?
d)?ampicillin?
6.?Which of the staining technique helps in demonstrating spore structure in bacteria as
well as free spores?
a)?Acid?fast?stain?
b)?Endospore?stain?
c)?Capsule?stain?
d)?Flagella?stain?
?
7.?In Gram-staining, iodine is used as a______________
a)?fixative?
b)?mordant?
c)?solublizer?
d)?stain?
8.?Which of the following is the example of Gram-negative bacteria?
a)?Lactobacillus?
b)?Eschericia?coli?
c)?Staphylococcus?aureus?
d)?Bacillus?subtilis?
9.?Which of the following is used in electron microscope?
a)?electron?beams?
b)?magnetic?fields?
c)?light?waves?
d)?electron?beams?and?magnetic?fields?
10.?Electron Microscope can give a magnification up to ___________
a)?400,000X?
b)?100,000X?
c)?15000X?
d)?100X?
11.?Which group of bacteria among the following have the largest area of contact?
a)?Palisades?
b)?Trichomes?
c)?Streptobacilli?
d)?Diplobacilli?
12.?Which of the following are functions of stalk?
a)?nutrient?absorption?
b)?motility?
c)?attachment?of?the?cells?to?surfaces?
d)?human?infection?
13.?Salmonella typhi has which type of flagella arrangement?
a)?Amphitrichous?
b)?Peritrichous?
c)?Monotrichous?
d)?Lophotrichous?
?
?
14.?The L Ring in a Gram-Negative bacterium flagella is associated with_________
a)?Peptidogycan?
b)?Outer?Membrane?
c)?Cytoplasmic?Membrane?
d)?Cell?Membrane?
?
15.?F pilus has a major role as___________
a)?motility?of?the?cell?
b)?port?of?entry?of?genetic?material?during?mating?
c)?attachment?to?host?cell?
d)?human?infection?
?
16. Prosthecae helps in __________
a)?motility?
b)?nutrient?absorption?and?attachment?to?surfaces?
c)?human?infection?
d)?protection?from?environment?
?
17. When a bacteria swim towards a chemical, it is termed as__________?
a)?positive?chemotaxis?
b)?phototaxis?
c)?negative?chemotaxis?
d)?magnetotaxis?
?
18.?Which type of force drives the flagellar motion?
a)?Protonmotive?force?
b)?ATP?driven?
c)?Protonmotive?and?ATP?driven?
d)?No?protonmotive?nor?ATP?driven?
?
19.?Peptidoglycan is made up of __________?
a)?N?acetylglucosamine?
b)?N?acetylmuramic?acid?
c)?N?acetylglucosamine,?N?acetylmuramic?acid?
d)?N?acetylglucosamine,N?acetylmuramicacid,amino?acids?
?
?
20.?Teichoic acid present in Gram-positive bacteria can bind to which ion?
a)?Fe?ions?
b)?Phosphorus?ions?
c)?Mg?ions?
d)?Sulphur?ions?
?
21?Cord factor is a ___________?
a)?protein?
b)?teichoic?acid?derivative?
c)?mycolic?acid?derivative?
d)?carbohydrate?
?
22.?The outer membarane of the Gram-negative cell wall is anchored to the underlying
peptidoglycan by means of which of the following?
a)?Braun?s?Lipoprotein?
b)?Phospholipids?
c)?Proteins?
d)?Lipopolysaccharide?
?
23.?NAG and NAM of peptidoglycan layer is linked by _________?
a)?beta?(1,4)?glycosidic?linkage?
b)?alpha?(1,4)?glycosidic?linkage?
c)?alpha?(1,6)?glycosidic?linkage?
d)?beta?(1,6)?glycosidic?linkage?
?
24.?Which of the following methods can be utilized for removing peripheral proteins of
the cytoplasmic membrane??
a)?treatment?by?detergents?
b)?osmotic?shock?
c)?heat?application?
d)?destruction?of?the?membrane?
?
25.?Which of the following are functions of water in the culture medium?
a)?nutrients?must?be?in?aqueous?solution?
b)?cofactor?of?enzymes?
c)?provides?resistance?to?sudden?transient?temperature?changes?
d)?it?is?a?chemical?reactant,?nutrients?must?also?be?present?in?aqueous?solution?and?provides?
resistance?to?sudden?temperature?changes.?
?
26.?The isolation of gonorrhea-causing organism, Neisseria gonorrhoeae by the use of
certain antibiotics in media is an example of which of the following?
a)?Selective?media?
b)?Differential?media?
c)?Enriched?media?
d)?Assay?media?
?
?
27.?Nutrient broth, a liquid media contains beef extract and peptone respectively in how
much amounts?
a)?0.2%,?0.4%?
b)?0.1%,?0.6%?
c)?0.3%,?0.5%?
d)?0.7%,?0.3%?
?
28. Which of the following instrument is used for sterilizing the media after it has been
prepared?
a)?Autoclave?
b)?Laminar?Air?Flow?Chamber?
c)?Inoculum?Needle?
d)?Incubator?
?
29.?Which of the following is a Complex media for fungal growth?
a)?Nutrient?broth?
b)?Luria?Bertani?media?
c)?Potato?Dextrose?Agar(PDA)?media?
d)?Mac?Conkey?Agar?media?
?
30.?Which of the following bacteria requires nicotinic acid as a growth factor in their
media?
a)?Proteus?vulgaris?
b)?Nitrosomonas?sp.?
c)?E.?coli?
d)?Leuconostocmesenteroides?
?
31.?Which of the following factors are responsible for the stability of thermophiles at high
temperatures?
a)?increased?leakage?of?cell?components?
b)?presence?of?large?no.?of?polar?amino?acids?and?alpha?helix?protein?
c)?thermal?stability?of?ribosomes?
d)?presence?of?Inositol?diphosphate?and?thermal?stability?of?ribosomes?
?
32.?The bacterium Staphylococcus aureus is which type of bacteria?
a)?Psychrophile?
b)?Mesophile?
c)?Thermophile?
d)?Mesophile?and?psychrophile?
?
?
FirstRanker.com - FirstRanker's Choice
Hemwati Nandan Bahuguna Uttarakhand Medical
Education University
Administrative?Block,?Govt.?Doon?Medical?College?Campus,?Dehrakhas,??
Patel?Nagar,?Dehradun???248001?
Phone:?0135???2723323?Fax:? 0135???2723323?
Website: www.hnbumu.ac.in? E?mail:?info.hnbumu@gmail.com
Ph.D. ENTRANCE EXAMINATION - 2020
Subject? :??Microbiology? ? ? ??????????? Duration:?01:30?Hrs.?
Date?? :??06.01.2020? ? ??????????? Place?:?Dehradun?
Max?Marks:?60?? ?
?
Student?Name:??????Invigilator?Name:??
?
Signature:???????Signature:?
1. The dye eosinate of methylene blue belongs to which group?
a)?Acidic?dye?
b)?Basic?dye?
c)?Neutral?dye?
d)?Oxazine?dye?
?
2.?What is the correct order of staining reagents in Gram-Staining?
a)?Crystal?violet,?alcohol,?iodine?solution,?safranin?
b)?Crystal?violet,?iodine?solution,?alcohol,?safranin?
c)?Crystal?violet,?safranin,?alcohol,?iodine?solution?
d)?Iodine?solution,?crystal?violet,?alcohol,?safranin?
?
3.?Which bacteria appears purple-violet colour after staining?
a)?Gram?positive?
b)?Gram?negative?
c)?Both?Gram?positive?and?Gram?negative?
d)?Neither?Gram?positive?nor?Gram?negative?
?
4.?Which of the following are true for Gram-negative bacteria?
a)?upon?alcohol?treatment,?the?permeability?of?the?cell?wall?increases?
b)?crystal?violet?iodine?(CV?I)?complex?is?extracted?
c)?pore?size?decreases?and?the?CV?I?complex?cannot?be?extracted?
d)?alcohol?treatment?increases?the?permeability?of?the?cell?wall?and?the?CV?I?complex?can?be?
extracted?
5.?Gram-positive bacteria are usually more susceptible to?
a)?streptomycin?
b)?tetracyclin?
c)?penicillin?
d)?ampicillin?
6.?Which of the staining technique helps in demonstrating spore structure in bacteria as
well as free spores?
a)?Acid?fast?stain?
b)?Endospore?stain?
c)?Capsule?stain?
d)?Flagella?stain?
?
7.?In Gram-staining, iodine is used as a______________
a)?fixative?
b)?mordant?
c)?solublizer?
d)?stain?
8.?Which of the following is the example of Gram-negative bacteria?
a)?Lactobacillus?
b)?Eschericia?coli?
c)?Staphylococcus?aureus?
d)?Bacillus?subtilis?
9.?Which of the following is used in electron microscope?
a)?electron?beams?
b)?magnetic?fields?
c)?light?waves?
d)?electron?beams?and?magnetic?fields?
10.?Electron Microscope can give a magnification up to ___________
a)?400,000X?
b)?100,000X?
c)?15000X?
d)?100X?
11.?Which group of bacteria among the following have the largest area of contact?
a)?Palisades?
b)?Trichomes?
c)?Streptobacilli?
d)?Diplobacilli?
12.?Which of the following are functions of stalk?
a)?nutrient?absorption?
b)?motility?
c)?attachment?of?the?cells?to?surfaces?
d)?human?infection?
13.?Salmonella typhi has which type of flagella arrangement?
a)?Amphitrichous?
b)?Peritrichous?
c)?Monotrichous?
d)?Lophotrichous?
?
?
14.?The L Ring in a Gram-Negative bacterium flagella is associated with_________
a)?Peptidogycan?
b)?Outer?Membrane?
c)?Cytoplasmic?Membrane?
d)?Cell?Membrane?
?
15.?F pilus has a major role as___________
a)?motility?of?the?cell?
b)?port?of?entry?of?genetic?material?during?mating?
c)?attachment?to?host?cell?
d)?human?infection?
?
16. Prosthecae helps in __________
a)?motility?
b)?nutrient?absorption?and?attachment?to?surfaces?
c)?human?infection?
d)?protection?from?environment?
?
17. When a bacteria swim towards a chemical, it is termed as__________?
a)?positive?chemotaxis?
b)?phototaxis?
c)?negative?chemotaxis?
d)?magnetotaxis?
?
18.?Which type of force drives the flagellar motion?
a)?Protonmotive?force?
b)?ATP?driven?
c)?Protonmotive?and?ATP?driven?
d)?No?protonmotive?nor?ATP?driven?
?
19.?Peptidoglycan is made up of __________?
a)?N?acetylglucosamine?
b)?N?acetylmuramic?acid?
c)?N?acetylglucosamine,?N?acetylmuramic?acid?
d)?N?acetylglucosamine,N?acetylmuramicacid,amino?acids?
?
?
20.?Teichoic acid present in Gram-positive bacteria can bind to which ion?
a)?Fe?ions?
b)?Phosphorus?ions?
c)?Mg?ions?
d)?Sulphur?ions?
?
21?Cord factor is a ___________?
a)?protein?
b)?teichoic?acid?derivative?
c)?mycolic?acid?derivative?
d)?carbohydrate?
?
22.?The outer membarane of the Gram-negative cell wall is anchored to the underlying
peptidoglycan by means of which of the following?
a)?Braun?s?Lipoprotein?
b)?Phospholipids?
c)?Proteins?
d)?Lipopolysaccharide?
?
23.?NAG and NAM of peptidoglycan layer is linked by _________?
a)?beta?(1,4)?glycosidic?linkage?
b)?alpha?(1,4)?glycosidic?linkage?
c)?alpha?(1,6)?glycosidic?linkage?
d)?beta?(1,6)?glycosidic?linkage?
?
24.?Which of the following methods can be utilized for removing peripheral proteins of
the cytoplasmic membrane??
a)?treatment?by?detergents?
b)?osmotic?shock?
c)?heat?application?
d)?destruction?of?the?membrane?
?
25.?Which of the following are functions of water in the culture medium?
a)?nutrients?must?be?in?aqueous?solution?
b)?cofactor?of?enzymes?
c)?provides?resistance?to?sudden?transient?temperature?changes?
d)?it?is?a?chemical?reactant,?nutrients?must?also?be?present?in?aqueous?solution?and?provides?
resistance?to?sudden?temperature?changes.?
?
26.?The isolation of gonorrhea-causing organism, Neisseria gonorrhoeae by the use of
certain antibiotics in media is an example of which of the following?
a)?Selective?media?
b)?Differential?media?
c)?Enriched?media?
d)?Assay?media?
?
?
27.?Nutrient broth, a liquid media contains beef extract and peptone respectively in how
much amounts?
a)?0.2%,?0.4%?
b)?0.1%,?0.6%?
c)?0.3%,?0.5%?
d)?0.7%,?0.3%?
?
28. Which of the following instrument is used for sterilizing the media after it has been
prepared?
a)?Autoclave?
b)?Laminar?Air?Flow?Chamber?
c)?Inoculum?Needle?
d)?Incubator?
?
29.?Which of the following is a Complex media for fungal growth?
a)?Nutrient?broth?
b)?Luria?Bertani?media?
c)?Potato?Dextrose?Agar(PDA)?media?
d)?Mac?Conkey?Agar?media?
?
30.?Which of the following bacteria requires nicotinic acid as a growth factor in their
media?
a)?Proteus?vulgaris?
b)?Nitrosomonas?sp.?
c)?E.?coli?
d)?Leuconostocmesenteroides?
?
31.?Which of the following factors are responsible for the stability of thermophiles at high
temperatures?
a)?increased?leakage?of?cell?components?
b)?presence?of?large?no.?of?polar?amino?acids?and?alpha?helix?protein?
c)?thermal?stability?of?ribosomes?
d)?presence?of?Inositol?diphosphate?and?thermal?stability?of?ribosomes?
?
32.?The bacterium Staphylococcus aureus is which type of bacteria?
a)?Psychrophile?
b)?Mesophile?
c)?Thermophile?
d)?Mesophile?and?psychrophile?
?
?
?
33.?The Reactive Oxygen Species(ROS) poduced by some bacteria are degraded by which
of the?following enzymes??
a)?Peroxidase?
b)?Lyase?
c)?Catalase?
d)?Superoxide?dismutase,?Catalase?and?Peroxidase?
?
34. HIV is transmitted by all of the following routes except:
a) Saliva?
b) Needle?prick?injury?
c) Blood?transmission
d) Sexual?intercourse
35. A rise in the anti-HBc immunoglobulin in a patient indicates
a)?Acute?infection?
b)?Carrier?state?
c)?Prodromal?phase?
d)?Convalescence?
?
36.?All of the following infections may be transmitted be dental instruments except:
a)?HIV?
b)?Hepatitis?C?
c)?Hepatitis?B?
d)??Hepatitis?E?
?
37.?The culture media used for cultivation of HSV is
a)?Chocolate?agar?
b)?Robertoson's?cooked?meat?broth?
c)?Chorioallantoic?membrane?
d)?Sabourad's?agar?
?
38.?The causative organism of syphilis is:
a)?Borreliaburgolorferi?
b)?Chalamydia?trachomatis?
c)?Leptospirainterrogans?
d)?Treponemapallidum?
?
39.?On a stained slide, clostridium tetani has the appearance of a:
a)?Bunch?of?a?
b)?Chain?of?beads?
c)??Drum?stick?
d)?Safely?pin?
?
40.?An oxidase-positive, gram-negative rod which produces a bluish-green pigment has
been grown in culture from a swab obtained from an infected burn wound. This organism
is susceptible to gentamicin, ticarcillin and tobramicin, but resistant to all the other
antibiotics which of the following organisms is it likely to be?
a)?Escherichia?coli?
b)?Klebsiellapnemoniae?
c)??Proteus?miribilis?
d)??Pseudomonas?aeruginosa?
?
?
?
FirstRanker.com - FirstRanker's Choice
Hemwati Nandan Bahuguna Uttarakhand Medical
Education University
Administrative?Block,?Govt.?Doon?Medical?College?Campus,?Dehrakhas,??
Patel?Nagar,?Dehradun???248001?
Phone:?0135???2723323?Fax:? 0135???2723323?
Website: www.hnbumu.ac.in? E?mail:?info.hnbumu@gmail.com
Ph.D. ENTRANCE EXAMINATION - 2020
Subject? :??Microbiology? ? ? ??????????? Duration:?01:30?Hrs.?
Date?? :??06.01.2020? ? ??????????? Place?:?Dehradun?
Max?Marks:?60?? ?
?
Student?Name:??????Invigilator?Name:??
?
Signature:???????Signature:?
1. The dye eosinate of methylene blue belongs to which group?
a)?Acidic?dye?
b)?Basic?dye?
c)?Neutral?dye?
d)?Oxazine?dye?
?
2.?What is the correct order of staining reagents in Gram-Staining?
a)?Crystal?violet,?alcohol,?iodine?solution,?safranin?
b)?Crystal?violet,?iodine?solution,?alcohol,?safranin?
c)?Crystal?violet,?safranin,?alcohol,?iodine?solution?
d)?Iodine?solution,?crystal?violet,?alcohol,?safranin?
?
3.?Which bacteria appears purple-violet colour after staining?
a)?Gram?positive?
b)?Gram?negative?
c)?Both?Gram?positive?and?Gram?negative?
d)?Neither?Gram?positive?nor?Gram?negative?
?
4.?Which of the following are true for Gram-negative bacteria?
a)?upon?alcohol?treatment,?the?permeability?of?the?cell?wall?increases?
b)?crystal?violet?iodine?(CV?I)?complex?is?extracted?
c)?pore?size?decreases?and?the?CV?I?complex?cannot?be?extracted?
d)?alcohol?treatment?increases?the?permeability?of?the?cell?wall?and?the?CV?I?complex?can?be?
extracted?
5.?Gram-positive bacteria are usually more susceptible to?
a)?streptomycin?
b)?tetracyclin?
c)?penicillin?
d)?ampicillin?
6.?Which of the staining technique helps in demonstrating spore structure in bacteria as
well as free spores?
a)?Acid?fast?stain?
b)?Endospore?stain?
c)?Capsule?stain?
d)?Flagella?stain?
?
7.?In Gram-staining, iodine is used as a______________
a)?fixative?
b)?mordant?
c)?solublizer?
d)?stain?
8.?Which of the following is the example of Gram-negative bacteria?
a)?Lactobacillus?
b)?Eschericia?coli?
c)?Staphylococcus?aureus?
d)?Bacillus?subtilis?
9.?Which of the following is used in electron microscope?
a)?electron?beams?
b)?magnetic?fields?
c)?light?waves?
d)?electron?beams?and?magnetic?fields?
10.?Electron Microscope can give a magnification up to ___________
a)?400,000X?
b)?100,000X?
c)?15000X?
d)?100X?
11.?Which group of bacteria among the following have the largest area of contact?
a)?Palisades?
b)?Trichomes?
c)?Streptobacilli?
d)?Diplobacilli?
12.?Which of the following are functions of stalk?
a)?nutrient?absorption?
b)?motility?
c)?attachment?of?the?cells?to?surfaces?
d)?human?infection?
13.?Salmonella typhi has which type of flagella arrangement?
a)?Amphitrichous?
b)?Peritrichous?
c)?Monotrichous?
d)?Lophotrichous?
?
?
14.?The L Ring in a Gram-Negative bacterium flagella is associated with_________
a)?Peptidogycan?
b)?Outer?Membrane?
c)?Cytoplasmic?Membrane?
d)?Cell?Membrane?
?
15.?F pilus has a major role as___________
a)?motility?of?the?cell?
b)?port?of?entry?of?genetic?material?during?mating?
c)?attachment?to?host?cell?
d)?human?infection?
?
16. Prosthecae helps in __________
a)?motility?
b)?nutrient?absorption?and?attachment?to?surfaces?
c)?human?infection?
d)?protection?from?environment?
?
17. When a bacteria swim towards a chemical, it is termed as__________?
a)?positive?chemotaxis?
b)?phototaxis?
c)?negative?chemotaxis?
d)?magnetotaxis?
?
18.?Which type of force drives the flagellar motion?
a)?Protonmotive?force?
b)?ATP?driven?
c)?Protonmotive?and?ATP?driven?
d)?No?protonmotive?nor?ATP?driven?
?
19.?Peptidoglycan is made up of __________?
a)?N?acetylglucosamine?
b)?N?acetylmuramic?acid?
c)?N?acetylglucosamine,?N?acetylmuramic?acid?
d)?N?acetylglucosamine,N?acetylmuramicacid,amino?acids?
?
?
20.?Teichoic acid present in Gram-positive bacteria can bind to which ion?
a)?Fe?ions?
b)?Phosphorus?ions?
c)?Mg?ions?
d)?Sulphur?ions?
?
21?Cord factor is a ___________?
a)?protein?
b)?teichoic?acid?derivative?
c)?mycolic?acid?derivative?
d)?carbohydrate?
?
22.?The outer membarane of the Gram-negative cell wall is anchored to the underlying
peptidoglycan by means of which of the following?
a)?Braun?s?Lipoprotein?
b)?Phospholipids?
c)?Proteins?
d)?Lipopolysaccharide?
?
23.?NAG and NAM of peptidoglycan layer is linked by _________?
a)?beta?(1,4)?glycosidic?linkage?
b)?alpha?(1,4)?glycosidic?linkage?
c)?alpha?(1,6)?glycosidic?linkage?
d)?beta?(1,6)?glycosidic?linkage?
?
24.?Which of the following methods can be utilized for removing peripheral proteins of
the cytoplasmic membrane??
a)?treatment?by?detergents?
b)?osmotic?shock?
c)?heat?application?
d)?destruction?of?the?membrane?
?
25.?Which of the following are functions of water in the culture medium?
a)?nutrients?must?be?in?aqueous?solution?
b)?cofactor?of?enzymes?
c)?provides?resistance?to?sudden?transient?temperature?changes?
d)?it?is?a?chemical?reactant,?nutrients?must?also?be?present?in?aqueous?solution?and?provides?
resistance?to?sudden?temperature?changes.?
?
26.?The isolation of gonorrhea-causing organism, Neisseria gonorrhoeae by the use of
certain antibiotics in media is an example of which of the following?
a)?Selective?media?
b)?Differential?media?
c)?Enriched?media?
d)?Assay?media?
?
?
27.?Nutrient broth, a liquid media contains beef extract and peptone respectively in how
much amounts?
a)?0.2%,?0.4%?
b)?0.1%,?0.6%?
c)?0.3%,?0.5%?
d)?0.7%,?0.3%?
?
28. Which of the following instrument is used for sterilizing the media after it has been
prepared?
a)?Autoclave?
b)?Laminar?Air?Flow?Chamber?
c)?Inoculum?Needle?
d)?Incubator?
?
29.?Which of the following is a Complex media for fungal growth?
a)?Nutrient?broth?
b)?Luria?Bertani?media?
c)?Potato?Dextrose?Agar(PDA)?media?
d)?Mac?Conkey?Agar?media?
?
30.?Which of the following bacteria requires nicotinic acid as a growth factor in their
media?
a)?Proteus?vulgaris?
b)?Nitrosomonas?sp.?
c)?E.?coli?
d)?Leuconostocmesenteroides?
?
31.?Which of the following factors are responsible for the stability of thermophiles at high
temperatures?
a)?increased?leakage?of?cell?components?
b)?presence?of?large?no.?of?polar?amino?acids?and?alpha?helix?protein?
c)?thermal?stability?of?ribosomes?
d)?presence?of?Inositol?diphosphate?and?thermal?stability?of?ribosomes?
?
32.?The bacterium Staphylococcus aureus is which type of bacteria?
a)?Psychrophile?
b)?Mesophile?
c)?Thermophile?
d)?Mesophile?and?psychrophile?
?
?
?
33.?The Reactive Oxygen Species(ROS) poduced by some bacteria are degraded by which
of the?following enzymes??
a)?Peroxidase?
b)?Lyase?
c)?Catalase?
d)?Superoxide?dismutase,?Catalase?and?Peroxidase?
?
34. HIV is transmitted by all of the following routes except:
a) Saliva?
b) Needle?prick?injury?
c) Blood?transmission
d) Sexual?intercourse
35. A rise in the anti-HBc immunoglobulin in a patient indicates
a)?Acute?infection?
b)?Carrier?state?
c)?Prodromal?phase?
d)?Convalescence?
?
36.?All of the following infections may be transmitted be dental instruments except:
a)?HIV?
b)?Hepatitis?C?
c)?Hepatitis?B?
d)??Hepatitis?E?
?
37.?The culture media used for cultivation of HSV is
a)?Chocolate?agar?
b)?Robertoson's?cooked?meat?broth?
c)?Chorioallantoic?membrane?
d)?Sabourad's?agar?
?
38.?The causative organism of syphilis is:
a)?Borreliaburgolorferi?
b)?Chalamydia?trachomatis?
c)?Leptospirainterrogans?
d)?Treponemapallidum?
?
39.?On a stained slide, clostridium tetani has the appearance of a:
a)?Bunch?of?a?
b)?Chain?of?beads?
c)??Drum?stick?
d)?Safely?pin?
?
40.?An oxidase-positive, gram-negative rod which produces a bluish-green pigment has
been grown in culture from a swab obtained from an infected burn wound. This organism
is susceptible to gentamicin, ticarcillin and tobramicin, but resistant to all the other
antibiotics which of the following organisms is it likely to be?
a)?Escherichia?coli?
b)?Klebsiellapnemoniae?
c)??Proteus?miribilis?
d)??Pseudomonas?aeruginosa?
?
?
?
41.?In initial stages of tyhoid, salmonella can be detected in:
a)?Feacus?
b)?Urine?
c)?Blood?
d)All?of?the?above?
?
42.?Signs of tyhoid fever is/are
a)?Haemorrhage?
b)?perforation?
c)?Osteomyelitis?
d)?All?of?the?above?
?
43.?All of the following are true of streptococcus except:
a)?It's?group?of?lactic?acid?production?bacteria?
b)?Shows?adherence?to?dental?enamel?
c)??Most?common?in?humans?
d)?Most?common?is?animals?
?
44.? Electron microscopic examination of the bacterial flora of necrotising ulcerative
gingivitis indicates the presence of microorganisms within non-necrotic tissues in
advance of other bacteria. The organisms involved are:
a)?Cocci?
b)?Spirochetes?
c)?Bacteriophages?
d)?Filamentous?rods?
?
45.?The following are applicable to bacterial genoms except:
a)???It?is?composed?of?DNA?
b) It?doe?not?contain?histones?
c) It?is?circular?
d) Its?DNA?has?both?introns?and?extrons?
46.?The commonest method of detection of diptheria carriers is:
a)?Schick?test?
b)?Dick?test?
c)?Casoni's?test?
d)?Charles'?test?
47.? Each of the following can cause food poisoning except:
a)?Cl.?Difficile?
b)?Staphylococcus?
c)?Cl.?Welchii?
d)?Cl.?botulinum
?
48.?Germ tubes are formed mainly by:
a) Candida albicans
b) Candida stellatoidea
c) Candida tropicalis
d) Candida pseudotropicalls
?
49. Fungus capable to forming fungal ball is:
a) Penicillium
b) Aspergillus
c) Mucor
d) Rhizopus
FirstRanker.com - FirstRanker's Choice
Hemwati Nandan Bahuguna Uttarakhand Medical
Education University
Administrative?Block,?Govt.?Doon?Medical?College?Campus,?Dehrakhas,??
Patel?Nagar,?Dehradun???248001?
Phone:?0135???2723323?Fax:? 0135???2723323?
Website: www.hnbumu.ac.in? E?mail:?info.hnbumu@gmail.com
Ph.D. ENTRANCE EXAMINATION - 2020
Subject? :??Microbiology? ? ? ??????????? Duration:?01:30?Hrs.?
Date?? :??06.01.2020? ? ??????????? Place?:?Dehradun?
Max?Marks:?60?? ?
?
Student?Name:??????Invigilator?Name:??
?
Signature:???????Signature:?
1. The dye eosinate of methylene blue belongs to which group?
a)?Acidic?dye?
b)?Basic?dye?
c)?Neutral?dye?
d)?Oxazine?dye?
?
2.?What is the correct order of staining reagents in Gram-Staining?
a)?Crystal?violet,?alcohol,?iodine?solution,?safranin?
b)?Crystal?violet,?iodine?solution,?alcohol,?safranin?
c)?Crystal?violet,?safranin,?alcohol,?iodine?solution?
d)?Iodine?solution,?crystal?violet,?alcohol,?safranin?
?
3.?Which bacteria appears purple-violet colour after staining?
a)?Gram?positive?
b)?Gram?negative?
c)?Both?Gram?positive?and?Gram?negative?
d)?Neither?Gram?positive?nor?Gram?negative?
?
4.?Which of the following are true for Gram-negative bacteria?
a)?upon?alcohol?treatment,?the?permeability?of?the?cell?wall?increases?
b)?crystal?violet?iodine?(CV?I)?complex?is?extracted?
c)?pore?size?decreases?and?the?CV?I?complex?cannot?be?extracted?
d)?alcohol?treatment?increases?the?permeability?of?the?cell?wall?and?the?CV?I?complex?can?be?
extracted?
5.?Gram-positive bacteria are usually more susceptible to?
a)?streptomycin?
b)?tetracyclin?
c)?penicillin?
d)?ampicillin?
6.?Which of the staining technique helps in demonstrating spore structure in bacteria as
well as free spores?
a)?Acid?fast?stain?
b)?Endospore?stain?
c)?Capsule?stain?
d)?Flagella?stain?
?
7.?In Gram-staining, iodine is used as a______________
a)?fixative?
b)?mordant?
c)?solublizer?
d)?stain?
8.?Which of the following is the example of Gram-negative bacteria?
a)?Lactobacillus?
b)?Eschericia?coli?
c)?Staphylococcus?aureus?
d)?Bacillus?subtilis?
9.?Which of the following is used in electron microscope?
a)?electron?beams?
b)?magnetic?fields?
c)?light?waves?
d)?electron?beams?and?magnetic?fields?
10.?Electron Microscope can give a magnification up to ___________
a)?400,000X?
b)?100,000X?
c)?15000X?
d)?100X?
11.?Which group of bacteria among the following have the largest area of contact?
a)?Palisades?
b)?Trichomes?
c)?Streptobacilli?
d)?Diplobacilli?
12.?Which of the following are functions of stalk?
a)?nutrient?absorption?
b)?motility?
c)?attachment?of?the?cells?to?surfaces?
d)?human?infection?
13.?Salmonella typhi has which type of flagella arrangement?
a)?Amphitrichous?
b)?Peritrichous?
c)?Monotrichous?
d)?Lophotrichous?
?
?
14.?The L Ring in a Gram-Negative bacterium flagella is associated with_________
a)?Peptidogycan?
b)?Outer?Membrane?
c)?Cytoplasmic?Membrane?
d)?Cell?Membrane?
?
15.?F pilus has a major role as___________
a)?motility?of?the?cell?
b)?port?of?entry?of?genetic?material?during?mating?
c)?attachment?to?host?cell?
d)?human?infection?
?
16. Prosthecae helps in __________
a)?motility?
b)?nutrient?absorption?and?attachment?to?surfaces?
c)?human?infection?
d)?protection?from?environment?
?
17. When a bacteria swim towards a chemical, it is termed as__________?
a)?positive?chemotaxis?
b)?phototaxis?
c)?negative?chemotaxis?
d)?magnetotaxis?
?
18.?Which type of force drives the flagellar motion?
a)?Protonmotive?force?
b)?ATP?driven?
c)?Protonmotive?and?ATP?driven?
d)?No?protonmotive?nor?ATP?driven?
?
19.?Peptidoglycan is made up of __________?
a)?N?acetylglucosamine?
b)?N?acetylmuramic?acid?
c)?N?acetylglucosamine,?N?acetylmuramic?acid?
d)?N?acetylglucosamine,N?acetylmuramicacid,amino?acids?
?
?
20.?Teichoic acid present in Gram-positive bacteria can bind to which ion?
a)?Fe?ions?
b)?Phosphorus?ions?
c)?Mg?ions?
d)?Sulphur?ions?
?
21?Cord factor is a ___________?
a)?protein?
b)?teichoic?acid?derivative?
c)?mycolic?acid?derivative?
d)?carbohydrate?
?
22.?The outer membarane of the Gram-negative cell wall is anchored to the underlying
peptidoglycan by means of which of the following?
a)?Braun?s?Lipoprotein?
b)?Phospholipids?
c)?Proteins?
d)?Lipopolysaccharide?
?
23.?NAG and NAM of peptidoglycan layer is linked by _________?
a)?beta?(1,4)?glycosidic?linkage?
b)?alpha?(1,4)?glycosidic?linkage?
c)?alpha?(1,6)?glycosidic?linkage?
d)?beta?(1,6)?glycosidic?linkage?
?
24.?Which of the following methods can be utilized for removing peripheral proteins of
the cytoplasmic membrane??
a)?treatment?by?detergents?
b)?osmotic?shock?
c)?heat?application?
d)?destruction?of?the?membrane?
?
25.?Which of the following are functions of water in the culture medium?
a)?nutrients?must?be?in?aqueous?solution?
b)?cofactor?of?enzymes?
c)?provides?resistance?to?sudden?transient?temperature?changes?
d)?it?is?a?chemical?reactant,?nutrients?must?also?be?present?in?aqueous?solution?and?provides?
resistance?to?sudden?temperature?changes.?
?
26.?The isolation of gonorrhea-causing organism, Neisseria gonorrhoeae by the use of
certain antibiotics in media is an example of which of the following?
a)?Selective?media?
b)?Differential?media?
c)?Enriched?media?
d)?Assay?media?
?
?
27.?Nutrient broth, a liquid media contains beef extract and peptone respectively in how
much amounts?
a)?0.2%,?0.4%?
b)?0.1%,?0.6%?
c)?0.3%,?0.5%?
d)?0.7%,?0.3%?
?
28. Which of the following instrument is used for sterilizing the media after it has been
prepared?
a)?Autoclave?
b)?Laminar?Air?Flow?Chamber?
c)?Inoculum?Needle?
d)?Incubator?
?
29.?Which of the following is a Complex media for fungal growth?
a)?Nutrient?broth?
b)?Luria?Bertani?media?
c)?Potato?Dextrose?Agar(PDA)?media?
d)?Mac?Conkey?Agar?media?
?
30.?Which of the following bacteria requires nicotinic acid as a growth factor in their
media?
a)?Proteus?vulgaris?
b)?Nitrosomonas?sp.?
c)?E.?coli?
d)?Leuconostocmesenteroides?
?
31.?Which of the following factors are responsible for the stability of thermophiles at high
temperatures?
a)?increased?leakage?of?cell?components?
b)?presence?of?large?no.?of?polar?amino?acids?and?alpha?helix?protein?
c)?thermal?stability?of?ribosomes?
d)?presence?of?Inositol?diphosphate?and?thermal?stability?of?ribosomes?
?
32.?The bacterium Staphylococcus aureus is which type of bacteria?
a)?Psychrophile?
b)?Mesophile?
c)?Thermophile?
d)?Mesophile?and?psychrophile?
?
?
?
33.?The Reactive Oxygen Species(ROS) poduced by some bacteria are degraded by which
of the?following enzymes??
a)?Peroxidase?
b)?Lyase?
c)?Catalase?
d)?Superoxide?dismutase,?Catalase?and?Peroxidase?
?
34. HIV is transmitted by all of the following routes except:
a) Saliva?
b) Needle?prick?injury?
c) Blood?transmission
d) Sexual?intercourse
35. A rise in the anti-HBc immunoglobulin in a patient indicates
a)?Acute?infection?
b)?Carrier?state?
c)?Prodromal?phase?
d)?Convalescence?
?
36.?All of the following infections may be transmitted be dental instruments except:
a)?HIV?
b)?Hepatitis?C?
c)?Hepatitis?B?
d)??Hepatitis?E?
?
37.?The culture media used for cultivation of HSV is
a)?Chocolate?agar?
b)?Robertoson's?cooked?meat?broth?
c)?Chorioallantoic?membrane?
d)?Sabourad's?agar?
?
38.?The causative organism of syphilis is:
a)?Borreliaburgolorferi?
b)?Chalamydia?trachomatis?
c)?Leptospirainterrogans?
d)?Treponemapallidum?
?
39.?On a stained slide, clostridium tetani has the appearance of a:
a)?Bunch?of?a?
b)?Chain?of?beads?
c)??Drum?stick?
d)?Safely?pin?
?
40.?An oxidase-positive, gram-negative rod which produces a bluish-green pigment has
been grown in culture from a swab obtained from an infected burn wound. This organism
is susceptible to gentamicin, ticarcillin and tobramicin, but resistant to all the other
antibiotics which of the following organisms is it likely to be?
a)?Escherichia?coli?
b)?Klebsiellapnemoniae?
c)??Proteus?miribilis?
d)??Pseudomonas?aeruginosa?
?
?
?
41.?In initial stages of tyhoid, salmonella can be detected in:
a)?Feacus?
b)?Urine?
c)?Blood?
d)All?of?the?above?
?
42.?Signs of tyhoid fever is/are
a)?Haemorrhage?
b)?perforation?
c)?Osteomyelitis?
d)?All?of?the?above?
?
43.?All of the following are true of streptococcus except:
a)?It's?group?of?lactic?acid?production?bacteria?
b)?Shows?adherence?to?dental?enamel?
c)??Most?common?in?humans?
d)?Most?common?is?animals?
?
44.? Electron microscopic examination of the bacterial flora of necrotising ulcerative
gingivitis indicates the presence of microorganisms within non-necrotic tissues in
advance of other bacteria. The organisms involved are:
a)?Cocci?
b)?Spirochetes?
c)?Bacteriophages?
d)?Filamentous?rods?
?
45.?The following are applicable to bacterial genoms except:
a)???It?is?composed?of?DNA?
b) It?doe?not?contain?histones?
c) It?is?circular?
d) Its?DNA?has?both?introns?and?extrons?
46.?The commonest method of detection of diptheria carriers is:
a)?Schick?test?
b)?Dick?test?
c)?Casoni's?test?
d)?Charles'?test?
47.? Each of the following can cause food poisoning except:
a)?Cl.?Difficile?
b)?Staphylococcus?
c)?Cl.?Welchii?
d)?Cl.?botulinum
?
48.?Germ tubes are formed mainly by:
a) Candida albicans
b) Candida stellatoidea
c) Candida tropicalis
d) Candida pseudotropicalls
?
49. Fungus capable to forming fungal ball is:
a) Penicillium
b) Aspergillus
c) Mucor
d) Rhizopus
?
50. A sporangium contains:
a) Spherules
b) Porangiospores
c) Chlamydospores
d) Oidia
51. Transmission of all of the following intestinal parasites takes place by the feco-oral
route except:
a) Giardia lambia
b) Ascaris lumbriocoides
c) Strongyloides
d) Entamoeba
52. Hydatid cyst is:
a) Parasitic in nature
b) Fungal
c) Congenital
d) Viral
53. Investigation of choice for invasive amoebiasis is:
a) Indirect hemagglutination
b) ELISA
c) Counter immune electroporesis
d) Microscopy
54. L.D bodies are seen in:
a) Kalahaza
b) Toxoplasmosis
c) Malaria
d) Sleeping sickness
55. Parasitic inflammation would show predominantly
a) Lymphocytes
b) Neutrophilis
c) Eosinophils
d) Basophils
56. Amoeb all are seen in a cysts of E. histolytica except:
a) Glycogen mass
b) Chromatid bars
c) Eccentric nucleus
d) Refractile nucleus
57. Example of immune complex hyper-sensitivity reaction is:
a) Atopic allergy
b) Serum sickness
c) Transfusion reaction
d) Contact dermatitis
58. Cysticercosis celluosae is caused by:
a) T. solium.
b) Echinococcus granulosus
c) T. Saginata
d) H. nana
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Hemwati Nandan Bahuguna Uttarakhand Medical
Education University
Administrative?Block,?Govt.?Doon?Medical?College?Campus,?Dehrakhas,??
Patel?Nagar,?Dehradun???248001?
Phone:?0135???2723323?Fax:? 0135???2723323?
Website: www.hnbumu.ac.in? E?mail:?info.hnbumu@gmail.com
Ph.D. ENTRANCE EXAMINATION - 2020
Subject? :??Microbiology? ? ? ??????????? Duration:?01:30?Hrs.?
Date?? :??06.01.2020? ? ??????????? Place?:?Dehradun?
Max?Marks:?60?? ?
?
Student?Name:??????Invigilator?Name:??
?
Signature:???????Signature:?
1. The dye eosinate of methylene blue belongs to which group?
a)?Acidic?dye?
b)?Basic?dye?
c)?Neutral?dye?
d)?Oxazine?dye?
?
2.?What is the correct order of staining reagents in Gram-Staining?
a)?Crystal?violet,?alcohol,?iodine?solution,?safranin?
b)?Crystal?violet,?iodine?solution,?alcohol,?safranin?
c)?Crystal?violet,?safranin,?alcohol,?iodine?solution?
d)?Iodine?solution,?crystal?violet,?alcohol,?safranin?
?
3.?Which bacteria appears purple-violet colour after staining?
a)?Gram?positive?
b)?Gram?negative?
c)?Both?Gram?positive?and?Gram?negative?
d)?Neither?Gram?positive?nor?Gram?negative?
?
4.?Which of the following are true for Gram-negative bacteria?
a)?upon?alcohol?treatment,?the?permeability?of?the?cell?wall?increases?
b)?crystal?violet?iodine?(CV?I)?complex?is?extracted?
c)?pore?size?decreases?and?the?CV?I?complex?cannot?be?extracted?
d)?alcohol?treatment?increases?the?permeability?of?the?cell?wall?and?the?CV?I?complex?can?be?
extracted?
5.?Gram-positive bacteria are usually more susceptible to?
a)?streptomycin?
b)?tetracyclin?
c)?penicillin?
d)?ampicillin?
6.?Which of the staining technique helps in demonstrating spore structure in bacteria as
well as free spores?
a)?Acid?fast?stain?
b)?Endospore?stain?
c)?Capsule?stain?
d)?Flagella?stain?
?
7.?In Gram-staining, iodine is used as a______________
a)?fixative?
b)?mordant?
c)?solublizer?
d)?stain?
8.?Which of the following is the example of Gram-negative bacteria?
a)?Lactobacillus?
b)?Eschericia?coli?
c)?Staphylococcus?aureus?
d)?Bacillus?subtilis?
9.?Which of the following is used in electron microscope?
a)?electron?beams?
b)?magnetic?fields?
c)?light?waves?
d)?electron?beams?and?magnetic?fields?
10.?Electron Microscope can give a magnification up to ___________
a)?400,000X?
b)?100,000X?
c)?15000X?
d)?100X?
11.?Which group of bacteria among the following have the largest area of contact?
a)?Palisades?
b)?Trichomes?
c)?Streptobacilli?
d)?Diplobacilli?
12.?Which of the following are functions of stalk?
a)?nutrient?absorption?
b)?motility?
c)?attachment?of?the?cells?to?surfaces?
d)?human?infection?
13.?Salmonella typhi has which type of flagella arrangement?
a)?Amphitrichous?
b)?Peritrichous?
c)?Monotrichous?
d)?Lophotrichous?
?
?
14.?The L Ring in a Gram-Negative bacterium flagella is associated with_________
a)?Peptidogycan?
b)?Outer?Membrane?
c)?Cytoplasmic?Membrane?
d)?Cell?Membrane?
?
15.?F pilus has a major role as___________
a)?motility?of?the?cell?
b)?port?of?entry?of?genetic?material?during?mating?
c)?attachment?to?host?cell?
d)?human?infection?
?
16. Prosthecae helps in __________
a)?motility?
b)?nutrient?absorption?and?attachment?to?surfaces?
c)?human?infection?
d)?protection?from?environment?
?
17. When a bacteria swim towards a chemical, it is termed as__________?
a)?positive?chemotaxis?
b)?phototaxis?
c)?negative?chemotaxis?
d)?magnetotaxis?
?
18.?Which type of force drives the flagellar motion?
a)?Protonmotive?force?
b)?ATP?driven?
c)?Protonmotive?and?ATP?driven?
d)?No?protonmotive?nor?ATP?driven?
?
19.?Peptidoglycan is made up of __________?
a)?N?acetylglucosamine?
b)?N?acetylmuramic?acid?
c)?N?acetylglucosamine,?N?acetylmuramic?acid?
d)?N?acetylglucosamine,N?acetylmuramicacid,amino?acids?
?
?
20.?Teichoic acid present in Gram-positive bacteria can bind to which ion?
a)?Fe?ions?
b)?Phosphorus?ions?
c)?Mg?ions?
d)?Sulphur?ions?
?
21?Cord factor is a ___________?
a)?protein?
b)?teichoic?acid?derivative?
c)?mycolic?acid?derivative?
d)?carbohydrate?
?
22.?The outer membarane of the Gram-negative cell wall is anchored to the underlying
peptidoglycan by means of which of the following?
a)?Braun?s?Lipoprotein?
b)?Phospholipids?
c)?Proteins?
d)?Lipopolysaccharide?
?
23.?NAG and NAM of peptidoglycan layer is linked by _________?
a)?beta?(1,4)?glycosidic?linkage?
b)?alpha?(1,4)?glycosidic?linkage?
c)?alpha?(1,6)?glycosidic?linkage?
d)?beta?(1,6)?glycosidic?linkage?
?
24.?Which of the following methods can be utilized for removing peripheral proteins of
the cytoplasmic membrane??
a)?treatment?by?detergents?
b)?osmotic?shock?
c)?heat?application?
d)?destruction?of?the?membrane?
?
25.?Which of the following are functions of water in the culture medium?
a)?nutrients?must?be?in?aqueous?solution?
b)?cofactor?of?enzymes?
c)?provides?resistance?to?sudden?transient?temperature?changes?
d)?it?is?a?chemical?reactant,?nutrients?must?also?be?present?in?aqueous?solution?and?provides?
resistance?to?sudden?temperature?changes.?
?
26.?The isolation of gonorrhea-causing organism, Neisseria gonorrhoeae by the use of
certain antibiotics in media is an example of which of the following?
a)?Selective?media?
b)?Differential?media?
c)?Enriched?media?
d)?Assay?media?
?
?
27.?Nutrient broth, a liquid media contains beef extract and peptone respectively in how
much amounts?
a)?0.2%,?0.4%?
b)?0.1%,?0.6%?
c)?0.3%,?0.5%?
d)?0.7%,?0.3%?
?
28. Which of the following instrument is used for sterilizing the media after it has been
prepared?
a)?Autoclave?
b)?Laminar?Air?Flow?Chamber?
c)?Inoculum?Needle?
d)?Incubator?
?
29.?Which of the following is a Complex media for fungal growth?
a)?Nutrient?broth?
b)?Luria?Bertani?media?
c)?Potato?Dextrose?Agar(PDA)?media?
d)?Mac?Conkey?Agar?media?
?
30.?Which of the following bacteria requires nicotinic acid as a growth factor in their
media?
a)?Proteus?vulgaris?
b)?Nitrosomonas?sp.?
c)?E.?coli?
d)?Leuconostocmesenteroides?
?
31.?Which of the following factors are responsible for the stability of thermophiles at high
temperatures?
a)?increased?leakage?of?cell?components?
b)?presence?of?large?no.?of?polar?amino?acids?and?alpha?helix?protein?
c)?thermal?stability?of?ribosomes?
d)?presence?of?Inositol?diphosphate?and?thermal?stability?of?ribosomes?
?
32.?The bacterium Staphylococcus aureus is which type of bacteria?
a)?Psychrophile?
b)?Mesophile?
c)?Thermophile?
d)?Mesophile?and?psychrophile?
?
?
?
33.?The Reactive Oxygen Species(ROS) poduced by some bacteria are degraded by which
of the?following enzymes??
a)?Peroxidase?
b)?Lyase?
c)?Catalase?
d)?Superoxide?dismutase,?Catalase?and?Peroxidase?
?
34. HIV is transmitted by all of the following routes except:
a) Saliva?
b) Needle?prick?injury?
c) Blood?transmission
d) Sexual?intercourse
35. A rise in the anti-HBc immunoglobulin in a patient indicates
a)?Acute?infection?
b)?Carrier?state?
c)?Prodromal?phase?
d)?Convalescence?
?
36.?All of the following infections may be transmitted be dental instruments except:
a)?HIV?
b)?Hepatitis?C?
c)?Hepatitis?B?
d)??Hepatitis?E?
?
37.?The culture media used for cultivation of HSV is
a)?Chocolate?agar?
b)?Robertoson's?cooked?meat?broth?
c)?Chorioallantoic?membrane?
d)?Sabourad's?agar?
?
38.?The causative organism of syphilis is:
a)?Borreliaburgolorferi?
b)?Chalamydia?trachomatis?
c)?Leptospirainterrogans?
d)?Treponemapallidum?
?
39.?On a stained slide, clostridium tetani has the appearance of a:
a)?Bunch?of?a?
b)?Chain?of?beads?
c)??Drum?stick?
d)?Safely?pin?
?
40.?An oxidase-positive, gram-negative rod which produces a bluish-green pigment has
been grown in culture from a swab obtained from an infected burn wound. This organism
is susceptible to gentamicin, ticarcillin and tobramicin, but resistant to all the other
antibiotics which of the following organisms is it likely to be?
a)?Escherichia?coli?
b)?Klebsiellapnemoniae?
c)??Proteus?miribilis?
d)??Pseudomonas?aeruginosa?
?
?
?
41.?In initial stages of tyhoid, salmonella can be detected in:
a)?Feacus?
b)?Urine?
c)?Blood?
d)All?of?the?above?
?
42.?Signs of tyhoid fever is/are
a)?Haemorrhage?
b)?perforation?
c)?Osteomyelitis?
d)?All?of?the?above?
?
43.?All of the following are true of streptococcus except:
a)?It's?group?of?lactic?acid?production?bacteria?
b)?Shows?adherence?to?dental?enamel?
c)??Most?common?in?humans?
d)?Most?common?is?animals?
?
44.? Electron microscopic examination of the bacterial flora of necrotising ulcerative
gingivitis indicates the presence of microorganisms within non-necrotic tissues in
advance of other bacteria. The organisms involved are:
a)?Cocci?
b)?Spirochetes?
c)?Bacteriophages?
d)?Filamentous?rods?
?
45.?The following are applicable to bacterial genoms except:
a)???It?is?composed?of?DNA?
b) It?doe?not?contain?histones?
c) It?is?circular?
d) Its?DNA?has?both?introns?and?extrons?
46.?The commonest method of detection of diptheria carriers is:
a)?Schick?test?
b)?Dick?test?
c)?Casoni's?test?
d)?Charles'?test?
47.? Each of the following can cause food poisoning except:
a)?Cl.?Difficile?
b)?Staphylococcus?
c)?Cl.?Welchii?
d)?Cl.?botulinum
?
48.?Germ tubes are formed mainly by:
a) Candida albicans
b) Candida stellatoidea
c) Candida tropicalis
d) Candida pseudotropicalls
?
49. Fungus capable to forming fungal ball is:
a) Penicillium
b) Aspergillus
c) Mucor
d) Rhizopus
?
50. A sporangium contains:
a) Spherules
b) Porangiospores
c) Chlamydospores
d) Oidia
51. Transmission of all of the following intestinal parasites takes place by the feco-oral
route except:
a) Giardia lambia
b) Ascaris lumbriocoides
c) Strongyloides
d) Entamoeba
52. Hydatid cyst is:
a) Parasitic in nature
b) Fungal
c) Congenital
d) Viral
53. Investigation of choice for invasive amoebiasis is:
a) Indirect hemagglutination
b) ELISA
c) Counter immune electroporesis
d) Microscopy
54. L.D bodies are seen in:
a) Kalahaza
b) Toxoplasmosis
c) Malaria
d) Sleeping sickness
55. Parasitic inflammation would show predominantly
a) Lymphocytes
b) Neutrophilis
c) Eosinophils
d) Basophils
56. Amoeb all are seen in a cysts of E. histolytica except:
a) Glycogen mass
b) Chromatid bars
c) Eccentric nucleus
d) Refractile nucleus
57. Example of immune complex hyper-sensitivity reaction is:
a) Atopic allergy
b) Serum sickness
c) Transfusion reaction
d) Contact dermatitis
58. Cysticercosis celluosae is caused by:
a) T. solium.
b) Echinococcus granulosus
c) T. Saginata
d) H. nana
59. The predominant immunoglobulin in saliva is:
a) IgA
b) IgD
c) IgE
d) IgG
60. Steam under pressure in used in ____ sterilization
a) Pasteurization
b) Autoclave
c) Kochs sterilizer
d) hot air oven
?
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?
?
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This post was last modified on 21 September 2020