Download OU B.Sc First Year 2019 Microbiology Question Bank Question Paper

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|'l?imc:2IIouhshrlax.Ivlnrks:50
IMajorl-lxperimcnt(Questions(lX20-20Nlarlas)
l.Alightcoinpound111lCl'O.'\'t?l)|)t?._stagerniciotnctci"andocularmicrontctcrtireprovidetoyou.

Calibratethemicroscopeforitsmeasurctnentinl0X(lowpower)and45X[lzighpower).

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Reportthecalibratedwtluesineachmagnification.
2.Acalibratedmicroscopearrangedwithtactilartnicrtxmetcrisprovidedtoyou.Preparea
slideofitiicrosctwtiiitutiiect(bacteriait:tang-a]spores)andmeasurethesize01'objectwith
tln:helpotkntiibratteitoculartnicioirtctcrin10wpowerandhighpower.Reporttheresult
antidcitiunstizttcyourOhSCfVdllOTl.

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(Note:Internalextiminerisrequiredtopro-calibratethemicroscopeandgivethe
calibratedvaluesintxmsultatiotiwithco-exztmittcr).
3.Abacterialpurt:eultureisprovidedtoyou.Preparethesntcarofsameatndstainby
differential(Grams)stainingrrzethtitl.Observethemicroscopiccharacteristicsofstained

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cultureantireportthemorphology,turrangcritu-rttandstainingnature.Demonstrateyour
ooscrvzition.
4.StainthegvuitbacterialL'Lt.llll'(?byendosptvrcstainingandreportyourtibscrvtationby
demonslrat..~'-_>_yourobsorvctiIicld.
u.

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Stainthegivenbacterialcultureby?agcllarstainingandreportyourobservationby
demonstratingyourobservedfield.'
6.Anactivelygrowingculture

providedtoyou.Prepareasmearandstainbycattsulc

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stainingtnethozlandreportyourobservation.
ll.Minorl-ZxperintontQuestions(IXI0-"10Marks]
7Demnitstrzttetheprcpztration-:>f?agzti'platesandslants.Explaintheirapplications
R.Demonstratestreak

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spreadplatetechniquefor"isolatingpuregulrureg,
9Demonsarepourplatetechnique.Fxpliuinitsadvantagesanddisadvantages
l0.Demonstrateseriall0Folddilutionmethodandextiluin.
ll.Abatcterietlcultuneis].\1'~JVl(.lC(ltoyou.Identifythetypeoftnicroorgaitismbyits
hiocltetnicalruaclit?.5.

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l2.Demortstrattcthegtenbacterialmotilitybyhatngingdropmethod.\?t-'i"?.tetlu:principle
.DBenn;"iciif?ia"
f,n
'maIILBOS
Dgtcgiftlllcrobiolvtllt

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Osmaniauniv?'$?Y~?*'?'

FirstRanker.com-FirstRanker'sChoice
FACELTYOFSCIENCE
B.Sc.IYearPracticalExamination2019-2020

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Stihjcct:NIICROBI()I.()CY(NewSyllabus)
Paper:I(IntroductoryMicrobiology)
QuestionBank
|'l?imc:2IIouhshrlax.Ivlnrks:50
IMajorl-lxperimcnt(Questions(lX20-20Nlarlas)

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l.Alightcoinpound111lCl'O.'\'t?l)|)t?._stagerniciotnctci"andocularmicrontctcrtireprovidetoyou.

Calibratethemicroscopeforitsmeasurctnentinl0X(lowpower)and45X[lzighpower).
Reportthecalibratedwtluesineachmagnification.
2.Acalibratedmicroscopearrangedwithtactilartnicrtxmetcrisprovidedtoyou.Preparea

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slideofitiicrosctwtiiitutiiect(bacteriait:tang-a]spores)andmeasurethesize01'objectwith
tln:helpotkntiibratteitoculartnicioirtctcrin10wpowerandhighpower.Reporttheresult
antidcitiunstizttcyourOhSCfVdllOTl.

(Note:Internalextiminerisrequiredtopro-calibratethemicroscopeandgivethe

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calibratedvaluesintxmsultatiotiwithco-exztmittcr).
3.Abacterialpurt:eultureisprovidedtoyou.Preparethesntcarofsameatndstainby
differential(Grams)stainingrrzethtitl.Observethemicroscopiccharacteristicsofstained
cultureantireportthemorphology,turrangcritu-rttandstainingnature.Demonstrateyour
ooscrvzition.

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4.StainthegvuitbacterialL'Lt.llll'(?byendosptvrcstainingandreportyourtibscrvtationby
demonslrat..~'-_>_yourobsorvctiIicld.
u.
Stainthegivenbacterialcultureby?agcllarstainingandreportyourobservationby
demonstratingyourobservedfield.'

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6.Anactivelygrowingculture

providedtoyou.Prepareasmearandstainbycattsulc
stainingtnethozlandreportyourobservation.
ll.Minorl-ZxperintontQuestions(IXI0-"10Marks]

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7Demnitstrzttetheprcpztration-:>f?agzti'platesandslants.Explaintheirapplications
R.Demonstratestreak

spreadplatetechniquefor"isolatingpuregulrureg,
9Demonsarepourplatetechnique.Fxpliuinitsadvantagesanddisadvantages

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l0.Demonstrateseriall0Folddilutionmethodandextiluin.
ll.Abatcterietlcultuneis].\1'~JVl(.lC(ltoyou.Identifythetypeoftnicroorgaitismbyits
hiocltetnicalruaclit?.5.
l2.Demortstrattcthegtenbacterialmotilitybyhatngingdropmethod.\?t-'i"?.tetlu:principle
.DBenn;"iciif?ia"

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f,n
'maIILBOS
Dgtcgiftlllcrobiolvtllt
Osmaniauniv?'$?Y~?*'?'

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ll.Specimenforspotting
I3
.Microscope
.4.
lnoculaliuvnIooyzfrlccdlc

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l5.Nutrientagarplate
l6.
IJFJIOIJPJ
~"?bl~J?
25.

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'.Nnstoc
.Spirulina
u-I
?I
.5?

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Nulriculagarskull
.AgardccpIubes
.Autoclave
.Ilotairoven
Filtration;aeppurmus

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.Streakplaic-
.Spreadplane
".Grampositiveluanilli
.Grzunpositivecocci
(iramnegativcbacilli

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.ekpcrgillus
.Rhizopus
.Sacclulrontyccs
.Pencillium
EleclrorlMicroscopicinwgeof'I?.\'I\/'

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HlecucnMicrosoupicimageoFH1\-?
.[minklcsl
T.Simmon?soilral:slant
Ill.Record&Viva
(5X3=1Sfvlarks)

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(5Marks)

FirstRanker.com-FirstRanker'sChoice
FACELTYOFSCIENCE
B.Sc.IYearPracticalExamination2019-2020

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Stihjcct:NIICROBI()I.()CY(NewSyllabus)
Paper:I(IntroductoryMicrobiology)
QuestionBank
|'l?imc:2IIouhshrlax.Ivlnrks:50
IMajorl-lxperimcnt(Questions(lX20-20Nlarlas)

--- Content provided by‍ FirstRanker.com ---

l.Alightcoinpound111lCl'O.'\'t?l)|)t?._stagerniciotnctci"andocularmicrontctcrtireprovidetoyou.

Calibratethemicroscopeforitsmeasurctnentinl0X(lowpower)and45X[lzighpower).
Reportthecalibratedwtluesineachmagnification.
2.Acalibratedmicroscopearrangedwithtactilartnicrtxmetcrisprovidedtoyou.Preparea

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slideofitiicrosctwtiiitutiiect(bacteriait:tang-a]spores)andmeasurethesize01'objectwith
tln:helpotkntiibratteitoculartnicioirtctcrin10wpowerandhighpower.Reporttheresult
antidcitiunstizttcyourOhSCfVdllOTl.

(Note:Internalextiminerisrequiredtopro-calibratethemicroscopeandgivethe

--- Content provided by FirstRanker.com ---

calibratedvaluesintxmsultatiotiwithco-exztmittcr).
3.Abacterialpurt:eultureisprovidedtoyou.Preparethesntcarofsameatndstainby
differential(Grams)stainingrrzethtitl.Observethemicroscopiccharacteristicsofstained
cultureantireportthemorphology,turrangcritu-rttandstainingnature.Demonstrateyour
ooscrvzition.

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4.StainthegvuitbacterialL'Lt.llll'(?byendosptvrcstainingandreportyourtibscrvtationby
demonslrat..~'-_>_yourobsorvctiIicld.
u.
Stainthegivenbacterialcultureby?agcllarstainingandreportyourobservationby
demonstratingyourobservedfield.'

--- Content provided by​ FirstRanker.com ---

6.Anactivelygrowingculture

providedtoyou.Prepareasmearandstainbycattsulc
stainingtnethozlandreportyourobservation.
ll.Minorl-ZxperintontQuestions(IXI0-"10Marks]

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7Demnitstrzttetheprcpztration-:>f?agzti'platesandslants.Explaintheirapplications
R.Demonstratestreak

spreadplatetechniquefor"isolatingpuregulrureg,
9Demonsarepourplatetechnique.Fxpliuinitsadvantagesanddisadvantages

--- Content provided by‌ FirstRanker.com ---

l0.Demonstrateseriall0Folddilutionmethodandextiluin.
ll.Abatcterietlcultuneis].\1'~JVl(.lC(ltoyou.Identifythetypeoftnicroorgaitismbyits
hiocltetnicalruaclit?.5.
l2.Demortstrattcthegtenbacterialmotilitybyhatngingdropmethod.\?t-'i"?.tetlu:principle
.DBenn;"iciif?ia"

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f,n
'maIILBOS
Dgtcgiftlllcrobiolvtllt
Osmaniauniv?'$?Y~?*'?'

--- Content provided by‌ FirstRanker.com ---

ll.Specimenforspotting
I3
.Microscope
.4.
lnoculaliuvnIooyzfrlccdlc

--- Content provided by​ FirstRanker.com ---

l5.Nutrientagarplate
l6.
IJFJIOIJPJ
~"?bl~J?
25.

--- Content provided by‌ FirstRanker.com ---

'.Nnstoc
.Spirulina
u-I
?I
.5?

--- Content provided by‌ FirstRanker.com ---

Nulriculagarskull
.AgardccpIubes
.Autoclave
.Ilotairoven
Filtration;aeppurmus

--- Content provided by FirstRanker.com ---

.Streakplaic-
.Spreadplane
".Grampositiveluanilli
.Grzunpositivecocci
(iramnegativcbacilli

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.ekpcrgillus
.Rhizopus
.Sacclulrontyccs
.Pencillium
EleclrorlMicroscopicinwgeof'I?.\'I\/'

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HlecucnMicrosoupicimageoFH1\-?
.[minklcsl
T.Simmon?soilral:slant
Ill.Record&Viva
(5X3=1Sfvlarks)

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(5Marks)

-
--..?--_-
FACULTY0FSCIENCE

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B.Sc.IYearPracticalExamination2019-2020
Subject:NIICROBIOLOGY(NewSyllabus)
PaperzI(IntroductoryMicrobiology)
N?adclQucstionPaper

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lime:3HOIIISNIQZQIIIZIfkSI50
I..\/']?jUl'l-ixpcrilnerltquestion(IXZO-?ZOMarks}
ll.MinorL-lxpcrirrlcntquestion(1XI0-1llh/Izlrks)
III.Spotters(5X3?'|Slvlarks)
IA)

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B)
c;
D)
.E)
I\-'.Record8:viva(5Marks)

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?FLAP-WIm
ChairmanBoS
DepLoIMicrobiology
OsmaniaUniversity)-,4,

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