Download OU (Osmania University) B.Sc Second Year (2nd Year) Microbiology Question Bank Question Paper
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B.Se.llSemester(Practical)ExaminatonJtHR
Subject:Microbiology
Paper-lI
QuestionBank
Time:2llrsMax.Marks:25
Note:Eachstudenthastoperformoneexperimentandfourspotters
I.ExperimentQuestion(l2Marks)
l.DemonsimteStreak/spreadplatetechniqueforisolatingpurecultures.
.Abacteriologicalculturemediumwithcolonies
is
providedto
you.
linumerzttethe
bacterialnumberandreportthenumberin(Fl-Ufgittufstail.
.Identifytheunknuw-nstigarspresentinthegivenmixturebyqualitativeanalysis.
Identifytheunknownaminoacidspresentinthegivenmixturebyqualitativeainalysis.
IdentifytheunknownaminoacidsinthegivenmixtureusingPaper(Ihromatography
IdentifytheunknownsugarsinthegivenmixtureusingPaperChromatography
FindouttheAbsorptionNlaximzitxfthcgivencolnurcdsolution.
.VerifyBeer-LumbcrtslawforcolouredsolutionllSingcalorimeter.
9.De?nebuffer.Prepareabuffersolutionwithspecificpllandreportitspll.
IQ
?.?$"P?-'
ll.SpecimensforSpotting(4x2=8Marks)
l.NutrientAgarplate
2.NutrientagarSlant
3.Agardeeptubes
4.PDA(Withlabeling)
5.Autoclave
6Hotairoven
7.Fillrzilionapparatus
8.Streakplate
9.
Spreadplate
1t.Mineraloiloverlaytube
ll.Colorillleter
l2.Papercliromatogram(aminoacids/sugars)
13.pHmeter-Estrips
M.lluller(labelled)
Ill.RecordandViva(5Marks)
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This post was last modified on 21 November 2019