QUESTIONBANK
FORPRACTICALS
Duration=2hoursTotal=25M
I.MAJORPRACTICALS1x10=10M
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1.?Qellfreezinandthawing2.Passagingofsuspensionandadherentcells
~3.1Qellyiabilityassay
4.Platingofcellsinmicrotiterplateatdefineddensity
ll.MINORPRACTIOALS1x5=5M
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1.Preparationofcellculturemedium2.Sterilizationmethodsincellculture
3.Trypanblueexclusionllestforoellvabilityanalysis
4.Cellcounting
lll.SPOTTERSIEXHIBITS5x1=5M
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1.Laminarairflow2.Cellcultureincubator
3.Liquidnitrogencontainer
4.Microscopyimagesofanimalcellculture
5.Flowchartforcryopreservation
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6.Schematicrepresentationofavectorwithclonedinsert7.Nucleartransfercloning
8.Isolationofbonemarrowstemcells
9.Flowchartofsouthernblotting
10.Schematicrepresentationofwesternblottingtechnique
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IV.RECORD8tVIVA5MB.ScGENETICSmYEAR
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semzstan-vDISCIPLINESPECIFICELECTIVE(nse)
DSE-(a)
PAPER:ANIMALceu.TECHNOLOGYaANIMALGENETICS
QUESTIONBANK
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FORPRACTICALSDuration=2hoursTotal=25M
I.MAJORPRACTICALS1x10=10M
1.?Qellfreezinandthawing
2.Passagingofsuspensionandadherentcells
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~3.1Qellyiabilityassay4.Platingofcellsinmicrotiterplateatdefineddensity
ll.MINORPRACTIOALS1x5=5M
1.Preparationofcellculturemedium
2.Sterilizationmethodsincellculture
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3.Trypanblueexclusionllestforoellvabilityanalysis4.Cellcounting
lll.SPOTTERSIEXHIBITS5x1=5M
1.Laminarairflow
2.Cellcultureincubator
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3.Liquidnitrogencontainer4.Microscopyimagesofanimalcellculture
5.Flowchartforcryopreservation
6.Schematicrepresentationofavectorwithclonedinsert
7.Nucleartransfercloning
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8.Isolationofbonemarrowstemcells9.Flowchartofsouthernblotting
10.Schematicrepresentationofwesternblottingtechnique
IV.RECORD8tVIVA5M
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1.2.
Ul&(e>
$?9?."$7?9?:5$*?N.*'QPWN?
Duratlon=2hours
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I.MAJORPRACTICALSll.MINORPRACTICALS
.Constructionofbardiagram,piediagram,linediagramforadata
.Constructionofhistogramandboxplotforadata
III.SPOTTERSIEXHIBITS
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IV.RECORD8:VIVAB.$?GENETICSIIIYEAR
SEMESTER-V
DISCIPLINESPECIFICCOURSE(DSC)
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PAPER:BIOSTATISTICS8rBIOINFORMATICSQUESTIONBANKFORPRACTICALS
To1al=25M
1x1O=10M
Problemsonmeasuresofcentraltendency(mean,medianandmode)
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Problemsonmeasuresofdispersionstandarddeviation,variance.standarderror,coeflicientofvariationforavariable
.ProblemsonhypothesistestingusingZtest,t-testandChi-squaredtest
.Problemsonprobabilityandprobabilitydistributions
SequenceretrievalfromGenbank/ENA
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6."SequenceretrievalfromSwissprot1x5=5M
Exploringwebportals?NCBI,EBI8rExPASy
LiteraturesearchthroughPubmedandPubmedCentral
PairwisehomologysearchbyBLASTandFASTA
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5x1=5MLinediagram,bardiagram3rpiediagrams
Histogram,frequencypolygon8rfrequencycurve
NormalProbablecurve
GenBank
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DDBJSWlSS-PROT
PROSITE
PIR
BLAST
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10.Pairwisealignment11.Multiplesequencealignment
12.PAMandBLOSUM
13.Phylogenetictree
5M
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B.ScGENETICSmYEAR
semzstan-v
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DISCIPLINESPECIFICELECTIVE(nse)DSE-(a)
PAPER:ANIMALceu.TECHNOLOGYaANIMALGENETICS
QUESTIONBANK
FORPRACTICALS
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Duration=2hoursTotal=25MI.MAJORPRACTICALS1x10=10M
1.?Qellfreezinandthawing
2.Passagingofsuspensionandadherentcells
~3.1Qellyiabilityassay
--- Content provided by FirstRanker.com ---
4.Platingofcellsinmicrotiterplateatdefineddensityll.MINORPRACTIOALS1x5=5M
1.Preparationofcellculturemedium
2.Sterilizationmethodsincellculture
3.Trypanblueexclusionllestforoellvabilityanalysis
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4.Cellcountinglll.SPOTTERSIEXHIBITS5x1=5M
1.Laminarairflow
2.Cellcultureincubator
3.Liquidnitrogencontainer
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4.Microscopyimagesofanimalcellculture5.Flowchartforcryopreservation
6.Schematicrepresentationofavectorwithclonedinsert
7.Nucleartransfercloning
8.Isolationofbonemarrowstemcells
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9.Flowchartofsouthernblotting10.Schematicrepresentationofwesternblottingtechnique
IV.RECORD8tVIVA5M
1.
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2.Ul&(e>
$?9?."$7?9?:5$*?N.*'QPWN?
Duratlon=2hours
I.MAJORPRACTICALS
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ll.MINORPRACTICALS.Constructionofbardiagram,piediagram,linediagramforadata
.Constructionofhistogramandboxplotforadata
III.SPOTTERSIEXHIBITS
IV.RECORD8:VIVA
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B.$?GENETICSIIIYEAR
SEMESTER-V
DISCIPLINESPECIFICCOURSE(DSC)
PAPER:BIOSTATISTICS8rBIOINFORMATICS
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QUESTIONBANKFORPRACTICALSTo1al=25M
1x1O=10M
Problemsonmeasuresofcentraltendency(mean,medianandmode)
Problemsonmeasuresofdispersionstandarddeviation,variance.standard
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error,coeflicientofvariationforavariable.ProblemsonhypothesistestingusingZtest,t-testandChi-squaredtest
.Problemsonprobabilityandprobabilitydistributions
SequenceretrievalfromGenbank/ENA
6."SequenceretrievalfromSwissprot
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1x5=5MExploringwebportals?NCBI,EBI8rExPASy
LiteraturesearchthroughPubmedandPubmedCentral
PairwisehomologysearchbyBLASTandFASTA
5x1=5M
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Linediagram,bardiagram3rpiediagramsHistogram,frequencypolygon8rfrequencycurve
NormalProbablecurve
GenBank
DDBJ
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SWlSS-PROTPROSITE
PIR
BLAST
10.Pairwisealignment
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11.Multiplesequencealignment12.PAMandBLOSUM
13.Phylogenetictree
5M
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B.ScGENETICSlllYEAR
SEMESTER-V
DISCIPLINESPECIFICELECTIVE{DSE-as)
DSE-3E(A)
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PAPER:PLANTGENEIICSANDBIOTECHNOLOGYQUESTIONBANKFORPRACTICALS
Duration=2hoursTotal=25M
.MAJORPRACTICALS1x10=10M
PreparationofMSMedia
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EstablishmentcfPrimaryCellCultureClonalPropagationfromaxillarybuds
Histologicalstudiesofembryosatdifferentstages
Preparationofsyntheticseedsfromsomaticembryos
999%"?
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II.MINORPRACTICALS1x5=5M
1.Introductiontoplanttissueculturelaboratoryequipment
2.ExplainvarioussterilizationmethodsusedintissueCulture
3.ExplainCallusinduction
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4.Explainseedtestingforgermination5.Describetheisolationofexplants
III.SPOTTERSIEXHIBITS5x1
=5M
.Chloroplastgenome
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.Mitochondrialenome.Somaticembryogenesis
Callus
Culturemedia
Explant
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Cellsuspensioncultures.Autoclave
.Laminarair?ow
10.Celldifferentiation
11.Meristem
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12.Protoplastculture13.Syntheticseeds
14.Antherculture
?PQNQF-IP-PUN?
IV.RECORD8iVIVA
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