RUHS
First Year MBBS Examination
I MBBS BIOCHEMISTRY PAPER II
Date: 16-03-2022
Instructions: INSTRUCTIONS: Attempt all

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questions in both sections: (Use separate
answer book for each section)
Section 1
Max Marks: 100
1. Fill in the blanks: (6)

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a. The Northern blot is used to demonstrate
specific ______________
b. Cereals are deficient in ______________
amino acid.
c. The positive direct van den Bergh's reaction

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indicates the presence of ______________
d. The Jumping gene is also known as
______________ element.
e. The normal value of urea clearance is
______________

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f. Hyperventilation is a compensatory
respiratory response to ______________
2. Choose the correct option in the following

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multiple choice questions: (4)
a. All of the following are the components of 'Pre

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initiation complex' Except;
a) Enhancers
b) TF ID
c) TATA box
d) RNAP II

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b. Which of the following is not a mechanism for
mutation of DNA?
a) Substitution of nucleotide
b) Dimerization of nucleotide
c) Insertion of nucleotide

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d) Deletion of nucleotide
c. Superoxide radicals can be detoxified by:
a) Cytochrome c
b) Cytochrome b
c) Cytochrome a

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d) None
d. The following enzyme is widely used in
recombinant DNA technology;
a) DNA polymerase
b) Telomerase

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c) Restriction Endonuclease
d) None
3. A 45-year male complained of severe joint pain

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especially in the big toes in the early morning
hours. The consultant decided to get his uric

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acid level in serum to be estimated which was
12.0 mg/dLHe was non-vegetarian and
alcoholic, he was asked to restrict the non-
vegetarian diet and alcohol (15)
a. What is the normal level of serum uric acid?

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b. What is the difference between primary gout
and secondary gout?
c. Explain the biochemical basis of the
symptoms in this patient.
d. How is the above disease treated?

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e. Why he was asked to restrict non-vegetarian
diet and alcohol?
4. Write short notes on: (10)
a. Post-transcriptional modifications.
b. Reactive oxygen species and its formation.

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c. Cytochrome P-450.
d. Lac operon concept.
e. DNA Probes.
5. Explain briefly (Any three): (15)
a. Application of PCR.

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b. Vitamin C is involved in the post-translational

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modification of collagen.
c. Vectors.
d. Classification of immunoglobulins.
Section 2

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1. Write an essay on biochemical bases of
protein-energy malnutrition. (20)
2. Explain Why (Any five): (10)
a. TSH is elevated in primary hypothyroidism.
b. UV radiation can cause Xeroderma

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Pigmentosum.
c. Methotrexate (Folic acid analogues) is used to
treat neoplastic disease.
d. Oedema occurs in Kwashiorkor.
e. Clay color stool in obstructive jaundice.

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f. Blue fluorescent light is useful in the treatment
of neonatal jaundice.
3. Explain briefly (Any four): (20)
a. Application of ELISA
b. DNA ligase is essential in lagging strand

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synthesis.
c. Restriction fragment length polymorphisms
(RFLPs).

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d. Elastin.
e. Oncogenes.

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