Subject Title: Microbial Physiology and Biochemistry
Semester: |l Updated on: 11.02.2020
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Year: |
Prepared by: Noor Jahan
Unit - I: Microbial nutrition & growth
Short Questions:
- Macro Nutrients & Micro Nutrients
- Growth factors
- Facilitated Diffusion
- Active Transport
- Group Translocation
- Photolithoautotrophs
- Chemolithoautotrophs
- Photo-organoheterotrophs
- Chemo-organoheterotrophs
- Methylotrophs & Mixotrophs
- Autotrophs & Heterotrophs
- Bacteriocholrophyll
- Photosynthetic Apparatus
- Batch Culture
- Continuous & Synchronous Culture
- Bi-Phasic(Diauixic Growth)
- Viable count & Direct Microscopic
- Turbidometry
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Essay Questions:
- What is nutrition? Give a detail account of nutritional requirement of bacteria.
- What is active transport? How bacteria uptake their nutrition by this mechanism.
- What is Facilitated diffusion? How bacteria uptake their nutrition by this mechanism.
- What is Group translocation? How bacteria uptake their nutrition by this mechanism
- Discuss the process of Photo-organoheterotrophs and Chemoorganoheterotrophs in detail.
- Discuss the process of Photoithoautotrophs and Chemolithoautotrophs in detail.
- Describe the photosynthetic apparatus in prokaryotes.
- Describe different phases of growth in Batch Culture of microorganism
- Describe various methods used to measuring Microbial growth.
- Briefly discuss the Factors Influencing Microbial growth.
- What is Biphasic growth? Why Synchronous growth system so useful to microorganism?
- What is Synchronous growth culture? Why Synchronous growth system so useful to microorganism?
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Unit - II: Microbial Metabolism
Short Questions:
- Chlorophylls & Bacteriocholrophyll
- Carotenoids & Phycobiliprotein
- Cyclic photophosphorylation
- Non-Cyclic photophosphorylation
- Anoxygenic photosynthesis
- Glycolysis
- Hexose Mono-Phosphate Pathway
- ED Pathway
- Anaplerotic Reaction
- Oxidative Phosphorylation
- Substrate Level Phosphorylation
- Glyoxylate Cycle
- Nitrate Respiration
- Sulphate Respiration
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Essay questions:
- Explain briefly about aerobic respiration?
- Explain briefly about anaerobic respiration?
- What do you understand by electron transport chain?
- Explain briefly about Glycolysis cycle?
- Explain briefly about HMP cycle?
- Write difference between Oxidative Phosphorylation and Substrate Level Phosphorylation?
- Explain briefly about E D pathway?
- Explain briefly about Glyoxylate Cycle
- Explain briefly about TCA cycle?
- Describe briefly about oxygenic photosynthesis.
- Describe briefly about anoxygenic photosynthesis
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Unit - lll: Biomolecules:
Short questions:
- Monosaccharides
- Oligossaccharides
- Polysaccharides
- Difference between phospholipids and glycolipids
- Sterols & Sphingolipids
- Phospholipids
- Nucleoside & Nucleotide
- Nitrogenous bases
- Amino Acids
- Secondary structure of proteins
- Enzyme Unit
- Lock and Key model
- Competitive Inhibition
- Un-Cometitive & Non-Cometitive inhibition
- Induced fit model
- Co-enzyme & Co-factor
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Essay Questions:
- What are Carbohydrate? Classify carbohydrates and describe their significance in biological system.
- What are lipids? Describe different categories of lipids and its significance
- Describe the general structure, classification and chemical properties of amino acids
- Give an account on different characteristics of proteins?
- Describe double helix model of DNA with the help of diagram. Briefly describe various-forms of DNA double helix
- Describe the structure and function of different types of RNA molecule
- Describe the structure and functions of nucleic acids?
- Give an account on Classification of Enzymes and Nomenclature of Enzyme?
- Give a detailed account in inhibition of enzyme activity.
- Give an account on properties of enzyme
- What is Bio-catalysis? Write in detail about the factor affecting the catalytic activity of enzyme?
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Unit - IV: Bio-Chemical techniques:
Short questions:
- Hydrogen ion concentration
- Beer - Lambert's law
- Buffers
- Paper Chromatography
- Thin layer chromatography
- Agarose gel electrophoresis
- SDS PAGE
- Colorimeter
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Essay Questions:
- What are buffers? Describe the role of buffers in biological reactions?
- Give an account on principles and applications of Colorimetry
- Describe paper chromatography in detail.
- Define pH and its measurement
- What is electrophoresis? Write its principles and application.
- Give an account on buffers in biological reactions and its preparation
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