Subject Title: Microbial Genetics and Molecular Biology
Prepared by: R.Jhansi
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Year: II Semester: IV Updated on: 23.03.19
Unit - I: Microbial Genetics
Short Answers:
- Alleles
- Linkage
- Chargaff's rule
- Plasmid
- Transposons
- Hershey and Chase experiment
- Crossing over
- RNA as genetic material
- Law of independent assortment
- Law of segregation
- Law of Dominance
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Long Answers:
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- Explain laws of Inheritance with examples
- Describe Watson and Crick model of DNA
- Explain Semi-conservative mode of replication
- Explain crossing over with mechanism
- Explain DNA as a genetic material.
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Unit - II: Mutations
Short Answers:
- Spontaneous mutation
- Base pair changes
- Deletion
- Insertion
- Inversion
- Frameshift mutation
- Tandem duplication
- Physical mutagen
- Excision repair
- Mismatch repair
- Recombinational repair
- SOS repair
- Chemical mutagen
- Transduction
- Conjugation
- Transformation
- DNA damage
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Long Answers:
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- Define mutation & describe the various classification of mutation
- List the various types of chemical mutagens and discuss the mechanism of action of chemical mutagens
- Describe the different types of DNA repair mechanism.
- Explain the mechanism of transduction.
- Explain gene transfer through conjugation in E.Coli.
- Describe in detail the mechanism of transformation
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Unit - III: Gene Expression
Short Answers:
- Muton
- Recon
- Cistron
- One gene-one enzyme hypothesis
- One gene-one polypeptide hypothesis
- One gene-one product hypothesis
- t-RNA
- r-RNA
- m-RNA
- Structural gene
- Regulatory gene
- Constitutive gene
- Wobble hypothesis
- Genetic code
- Operon
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Long Answers:
- Discuss the rho dependent and rho independent mechanism of transcription.
- Briefly describe the mode of protein synthesis in prokaryotes.
- What is genetic code? Explain its essential features
- Describe the structure and functions of different types of RNA.
- Discuss the concept of gene and explain the structural, constitutive and regulatory genes
- Define operon. Explain the operon concept mode of gene regulation using lac operon of E.coli as examples.
- Give a brief account on gene cloning method.
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Unit - IV: Recombinant DNA technology
Short Answers:
- Restriction Endonuclease
- DNA polymerases
- Ligases
- Vectors
- cDNA
- Genome libraries
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Long Answers:
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- Give a detailed account on molecular mechanism of DNA recombinant technology.
- Briefly describe the construction of genomic library and discuss its advantages.
- Briefly describe the construction of cDNA library and discuss its advantages.
- Give an account on application of genetic engineering in different fields.
- Explain basic principles of genetic engineering.
- Give an account on enzyme involved in genetic engineering and their mode of action.
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