OU B.Sc Life Sciences 2021 Important Question Bank || Osmania University (Important Questions)
Subject Title: Applied Biochemistry Prepared by: M Madhavi
Year: III Year Semester: V Sem Updated on: 19.08.
--- Content provided by FirstRanker.com ---
Unit - I: Enzyme and Protein purification methods
Essay Answer Type
- What is Homogenization and explain homogenization techniques.
- Discuss briefly about centrifugation methods.
- Explain the process of protein purification by ammonium sulphate precipitation.
- Describe briefly about column chromatography and determination of molecular weight.
- How to determine protein concentration by using UV spectra.
- Write the principle and procedure of SDS-PAGE.
- Explain about Native PAGE.
--- Content provided by FirstRanker.com ---
Short Answer Type
- Definition of Homogenization and write Applications of Homogenization.
- Differential centrifugation.
- Dialysis of proteins.
- Applications of column chromatography
- Principle involved in SDS-PAGE
- What is the difference between SDS-PAGE and Native PAGE.
--- Content provided by FirstRanker.com ---
--- Content provided by FirstRanker.com ---
Unit - ll: Nucleic acid analysis and Cell cultures
Essay Answer Type
- Write the principle and process of agarose gel electrophoresis.
- Write briefly about PCR and its applications.
- Explain about different types of blotting techniques.
- Discuss briefly about plant cell cultures.
- Discuss briefly about animal cell cultures.
- Write about Amylase producing microbial cultures.
- Explain cellulase production process by using microbial cultures.
- Explain the process of protease production by using microbial cultures.
--- Content provided by FirstRanker.com ---
Short Answer Type
--- Content provided by FirstRanker.com ---
- Principle involved in agarose gel electrophoresis.
- Applications of PCR
- Western blotting
- Southern blotting
- Plant cell cultures
- Northern blotting
- Animal cell cultures
- How amylase produced by microbial cultures?
- Applications of amylase
--- Content provided by FirstRanker.com ---
--- Content provided by FirstRanker.com ---
This download link is referred from the post: OU B.Sc Life Sciences 2021 Important Question Bank || Osmania University (Important Questions)
--- Content provided by FirstRanker.com ---