Download RGUHS (Rajiv Gandhi University of Health Sciences) B.Sc MLT 2018 March (Bachelors in Science in Medical Laboratory Technology) 2nd Year 3156 Biochemistry II Previous Question Paper
Rajiv Gandhi University of Health Sciences, Karnataka
II Year B.Sc. (M.L.T) Degree Examination ? MARCH 2018
Time: Three Hours
Max. Marks: 80 Marks
BIOCHEMISTRY ? II
Q.P. CODE: 3156
Your answers should be specific to the questions asked.
Draw neat labeled diagrams wherever necessary
LONG ESSAYS (Answer any Two)
2 x 10 = 20 Marks
1.
Discuss the IUBMB system of Enzyme classification with suitable examples. Describe any three
factors affecting velocity of enzyme catalysed reaction.
2.
Give a detailed account of the operation of TCA cycle. Discuss its regulation and energetics. Add
a note on its amphibolic role?
3.
Outline the pathway of catabolism of phenyl alanine. Discuss the biochemical abnormality,
clinical features and laboratory diagnosis of Phenylketonuria.
SHORT ESSAYS (Answer any Six)
6 x 5 = 30 Marks
4.
Explain the principle and procedure of Ammonium chloride loading test. Discuss the urinary PH
measurement and the interpretation.
5.
Paper chromatography-Principle and procedure for separation of amino acids.
6.
How is Oral GTT carried out? Explain the different types of curves obtained.
7.
Define "primary structure" of proteins. Explain the primary structure of insulin.
8.
Coenzyme form of Thiamin and its biochemical functions.
9.
Principle and uses of reflectance photometry.
10.
List out various preservatives used during urine collection. Mention three tests for abnormal
constituents of urine.
11.
Give the detailed procedure for carrying out Endogenous Creatinine Clearance test. Discuss the
clinical significance.
SHORT ANSWERS (Answer any Ten)
10 x 3 = 30 Marks
12.
How is deproteinisation carried out for blood Uric acid by Phosphotungstic acid method and
Glucose by Folin Wu method?
13.
Define Osmolality. Give the principle of Freezing point depression Osmometry.
14.
Describe the functions of DNA and RNA.
15.
What is a Glucometer? Discuss its use, advantages and disadvantages.
16.
Discuss different types of water grades used in the laboratory. Add a note on the NCCLS
specifications for laboratory water.
17.
What is Glycated HbA1c? Give its clinical significance.
18.
List out the physical characteristics of Normal urine. How is specific gravity of the urine
measured?
19.
Explain salting out of proteins with suitable example.
20.
Name three CSF components for biochemical examination. Give the principle of quantitative
estimation of any two analytes.
21.
Urea cycle
22.
Give the functions of Vitamin B12 and Folic acid.
23.
Define microalbuminuria. Give the biological reference intervals and the clinical significance.
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This post was last modified on 24 December 2022