Download PTU B.Tech 2021 Jan BT-Biotechnology 5th Sem 78236 Animal Cell Culture And Biotechnology Question Paper

Download PTU (Punjab Technical University) B.Tech (Bachelor of Technology) / BE (Bachelor of Engineering) 2021 January BT-Biotechnology 5th Sem 78236 Animal Cell Culture And Biotechnology Previous Question Paper

Roll No.
Total No. of Pages : 02
Total No. of Questions : 18
B.T ech. (BT ) (Sem.?5)
ANIMAL CELL CULTURE & BIOTECHNOLOGY
Subject Code : BTBT-502-18
M.Code : 78236
Time : 3 Hrs. Max. Marks : 60
INST RUCT ION TO CANDIDAT ES :
1 .
SECT ION-A is COMPULSORY cons is ting of TEN questions carrying TWO marks
each.
2 .
SECT ION-B c ontains F IVE questions c arrying FIVE marks eac h and s tud ents
have to atte mpt any FOUR q ues tions.
3 .
SECT ION-C contains THREE questions carrying T EN marks e ach and s tudents
have to atte mpt any T WO questio ns.
SECTION-A
Answer briefly :
1)
What is cell differentiation?
2)
What do you understand by a complete media?
3)
What is subculturing?
4)
Define labeling index.
5)
What do you understand by generation time?
6)
What are cell banks?
7)
What is molecular pharming?
8)
What are transgenic animals?
9)
Give three applications of animal tissue culture.
10) Define cell lines.
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SECTION-B
11) Explain the process of cell adhesion and proliferation.
12) Discuss the layout of an animal tissue culture laboratory.
13) Discuss various steps involved in preparation of primary culture.
14) Write a note on cell cloning and cell separation.
15) Explain various gene transfer technologies in mammalian cells.
SECTION-C
16) Give a detailed account of various culture media used in animal tissue culture.
17) What are the various techniques for measurement of viability and cytotoxicity?
18) Write short notes on :
a) Cryopreservation of cell lines
b) ES and microinjection.
NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any
page of Answer Sheet will lead to UMC against the Student.
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