Download PTU B.Tech 2021 Jan BT-Biotechnology 5th Sem 78236 Animal Cell Culture And Biotechnology Question Paper

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Total No. of Pages : 02
Total No. of Questions : 18

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B.Tech. (BT) (Sem.-5)

ANIMAL CELL CULTURE & BIOTECHNOLOGY
Subject Code : BTBT-502-18
M.Code : 78236
Time : 3 Hrs. Max. Marks : 60

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INSTRUCTION TO CANDIDATES :

1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students have to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students have to attempt any TWO questions.

SECTION-A

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Answer briefly :

1. What is cell differentiation?
2. What do you understand by a complete media?
3. What is subculturing?
4. Define labeling index.

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5. What do you understand by generation time?
6. What are cell banks?
7. What is molecular pharming?
8. What are transgenic animals?
9. Give three applications of animal tissue culture.

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10. Define cell lines.

SECTION-B

11. Explain the process of cell adhesion and proliferation.
12. Discuss the layout of an animal tissue culture laboratory.
13. Discuss various steps involved in preparation of primary culture.

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14. Write a note on cell cloning and cell separation.
15. Explain various gene transfer technologies in mammalian cells.

SECTION-C

16. Give a detailed account of various culture media used in animal tissue culture.
17. What are the various techniques for measurement of viability and cytotoxicity?

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18. Write short notes on :
    1. Cryopreservation of cell lines
    2. ES and microinjection.

NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any page of Answer Sheet will lead to UMC against the Student.

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