This download link is referred from the post: PTU B.Tech 5th Semester Last 10 Years 2011-2021 Previous Question Papers|| Punjab Technical University
Total No. of Pages : 02
Total No. of Questions : 18
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B.Tech. (BT) (Sem.-5)
ANIMAL CELL CULTURE & BIOTECHNOLOGY
Subject Code : BTBT-502-18
M.Code : 78236
Time : 3 Hrs. Max. Marks : 60
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INSTRUCTION TO CANDIDATES :
- SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks each.
- SECTION-B contains FIVE questions carrying FIVE marks each and students have to attempt any FOUR questions.
- SECTION-C contains THREE questions carrying TEN marks each and students have to attempt any TWO questions.
SECTION-A
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Answer briefly :
- What is cell differentiation?
- What do you understand by a complete media?
- What is subculturing?
- Define labeling index.
- What do you understand by generation time?
- What are cell banks?
- What is molecular pharming?
- What are transgenic animals?
- Give three applications of animal tissue culture.
- Define cell lines.
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SECTION-B
- Explain the process of cell adhesion and proliferation.
- Discuss the layout of an animal tissue culture laboratory.
- Discuss various steps involved in preparation of primary culture.
- Write a note on cell cloning and cell separation.
- Explain various gene transfer technologies in mammalian cells.
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SECTION-C
- Give a detailed account of various culture media used in animal tissue culture.
- What are the various techniques for measurement of viability and cytotoxicity?
- Write short notes on :
- Cryopreservation of cell lines
- ES and microinjection.
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NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any page of Answer Sheet will lead to UMC against the Student.
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This download link is referred from the post: PTU B.Tech 5th Semester Last 10 Years 2011-2021 Previous Question Papers|| Punjab Technical University