This download link is referred from the post: PTU B.Tech 7th Semester Last 10 Years Previous Question Papers|| Punjab Technical University 2011-2021
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Total No. of Pages : 02
Total No. of Questions : 18
B.Tech. (BT) (2012 to 2017) (Sem.−7)
BIOPROCESS TECHNOLOGY
Subject Code : BTBT-701
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M.Code: 71843
Time: 3 Hrs.
Max. Marks : 60
INSTRUCTIONS TO CANDIDATES :
- SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks each.
- SECTION-B contains FIVE questions carrying FIVE marks each and students have to attempt ANY FOUR questions.
- SECTION-C contains THREE questions carrying TEN marks each and students have to attempt ANY TWO questions.
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SECTION-A
Discuss briefly :
- Write down two basic differences between bioprocessing and chemical processing.
- What is mean by bioconversion process?
- Highlight at least one advantage and one disadvantage of batch over continuous fermentation process.
- Explain continuous cell line.
- When filter sterilization is recommended?
- Define Respiratory Quotient.
- In recent days, what problems associated with the bakers yeast growth in molasses encourage the choice of starchy materials from cereal grains as growth medium?
- What is the major difference between the primary metabolite and the secondary metabolite production in relation to the microbial growth phase?
- Explain feedback inhibition.
- What are major ways of strain development?
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SECTION-B
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- Thermal death of microorganisms in the liquid medium follows first order kinetics. If the initial cell concentration in the fermentation medium is 108 cells / ml and the final acceptable contamination level is 103 cells, for how long should 1.0 m3 medium be treated at temperature of 120 °C to achieve acceptable load? Consider the thermal deactivation rate constant at 120 °C is 0.23 / min.
- What are the basic features of a fermenter? Draw a schematic to indicate them.
- Comment on ‘diffusion limitation in immobilized enzyme system'.
- Briefly explain the 'Chelating Agent' and 'Growth Factor' in media design.
- Discuss chromatography technique in downstream processing.
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SECTION-C
- Describe ethanol fermentation.
- A strain of mold was grown in batch culture on glucose and the following data were obtained :
Time (h) Cell concentration (g/1) Glucose concentration (g/1) 0 1.25 100 9 2.45 97 16 5.1 90.4 23 10.5 76.9 30 22 48.1 34 33 20.6 36 37.5 9.38 40 41 0.63 - Calculate the maximum net specific growth rate.
- Calculate the apparent growth yield.
- What maximum cell concentration could one expect if 150 g of glucose were used with the same size inoculum?
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- Discuss the feedback inhibition problem relating the L-Lysine production. Describe L-Lysine production including the production process, different media sources and influencing factors and downstream processing.
NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any page of Answer Sheet will lead to UMC against the Student.
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This download link is referred from the post: PTU B.Tech 7th Semester Last 10 Years Previous Question Papers|| Punjab Technical University 2011-2021