Download NTRUHS (DR. N.T.R UNIVERSITY OF HEALTH SCIENCES) MBBS (Bachelor of Medicine and Bachelor of Surgery) 1st Year Biochemistry Syllabus
4) BIOCHEMISTRY
Syllabus of Biochemistry including Molecular Biology
(i) Goal :
The broad goal of the teaching of Biochemistry to undergraduate students is to make them
understand the scientific basis of the life processes at the molecular level and to orient them towards the
application of the acquired, in solving clinical problems.
(ii) Objectives:
A. Knowledge:
At the end of the course, the student shal be able to :
a) describe the molecular and functional organization of a cell and list its subcelluar
components;
b) delineate structure, function and inter-relationship of biomolecules and consequences of
deviation from normal;
c) summarize the fundamental aspects of enzymology and clinical application wherein
regulation of enzymatic activity is altered;
d) describe digestion and assimilation of nutrients and consequences of malnutrition;
e) integrate the various aspects of metabolism and their requlatory pathways;
f) explain the biochemical basis of inherited disorders with their associate sequelae;
g) describe mechanisms involved in maintenance of body fluid and pH homeostasis;
h) outline the molecular mechanisms of gene expression and regulation, the principles of
genetic engineering and their application of medicine;
i) summarize molecular concept of body defences and their application in medicine;
j) out line the biochemical basis of environmental health hazards, biochemical basis of
cancer and carcinogenesis;
k) familiarize with the principles of various conventional and specialized laboratory
investigations and instrumentation; analysis and interpretation of a given data;
l) suggest experiments to support theoretical concepts and clinical diagnosis;
B.SKILLS:
At the end of the course the student shall be able to:
a) make use of conventional techniques/instruments to perform biochemical analysis
relevant to clinical screening and diagnosis;
b) analyze and interpret investigative data;
c) demonstrate the skil s of solving scientific and clinical problems and decision making;
C.INTEGRATION:
The knowledge acquired in biochemistry shall help the students to integrate molecular
events with structure and function of the human body in health and disease.
52
(iii) Syllabus Of Biochemistry Total hours 240
Theory:
Sl. No. Name of the Unit
No. of Hours 120
1.
Introduction to biochemistry
1
2.
Cell- Molecular & functional organisation
2
3.
Chemistry of Carbohydrates:
5
a) Classification of Carbohydrates:
b) Structural and functional aspects of Mono-saccharides,
Disaccharides, Homo and Hetero Polysaccharides
4.
Chemistry of Lipids:
4
a) Classification
b) Structural and functional aspects of simple, compound and Derived lipids
including saturated, unsaturated and Essential Fatty aids.
5.
Chemistry of Proteins:
8
a) Classification & functional aspects.
b) Electrophoretic separation of proteins
c) Classification and Properties of amono aids
d) Separation of Amino acids by Chromatography
e) Outlines of elucidation of Protein Structure.
f) Biological y active Peptides
6.
Nucleic Acids:
4
a) Bases, nucleotides, Nucleic acids,(structural and functional aspects)
b) synthetic nucleotides
7.
Enzymes:
6
a) Classification
b) Mechanism of Enzyme action
c) Enzyme kinetics
d) Factors affecting enzyme activity
e) Isoenzymes
f) Coenzymes
g) Enzyme Inhibition
h) Cellular & Plasma enzymes
i) Diagnostic importance of Enzymes
j) Regulation of Enzyme activity
8. Biological Oxidation:
4
a) Bioenergetics
b) Exergonic & Endergonic reaction
c) Oxidases
d) Electron Transport Chain
e) Oxidative Phosphorylation
f) High energy Compounds
g) Low Energy Compounds
9. Vitamins:
10
a) Classification
b) Structure, Sources, Daily requirement,
Physiological role and deficiency disorders of Fat soluble vitamins ? A,D,E,& K and
water soluble vitamins-B. complex group and Vit. C.
53
10. Carbohydrate Metabolism:
10
a) Digestion
b) Absorption
c) Metabolism of Glucose
i)
Entry of Glucose into Cel s
ii)
Glycolysis
i i)
Rapaport ? Leubering Cycle
iv)
Pyruvate Dehydrogenase Complex
v)
Citric Acid Cycle
vi)
Gluconeogenesis
vii)
Glycogenesis
vii )
Glycogenolysis
ix)
Glycogen Storage Diseases
x)
Hexose Mono Phosphate Shunt Pathway
xi)
Uronic Acid Pathway
xi )
Metabolism of Galactose & Fructose
xi i)
Blood Glucose Homeostasis, Glucose Tolerance Test, Diabetes
Mellitus and Hypoglycemia
11.Metabolism of Proteins:
10
a) Protein Digestion & Absorption
b) General Pathways of metabolism including
c) Transamination & Deamination and Ammonia transport
d) Urea Cycle
e) Metabolism of individual amino acids & Molecular disorders.
f) Creatine & Creatinine
12. Metabolism of Nucleic Acids:
9
a) Outlines of Metabolism of Purines & Pyrimidines & Metabolic disorders
b) DNA replication and transcription
c) Protein Biosynthesis(Translation)
d) Regulation of Gene Expression
e) Outlines of Genetic Engineering
13. Lipid Metabolism :
9
a) Digestion & Absorption
b) Plasma Lipids
c) Mobilisation of Fats from adipose tissue
d) Oxidation of Fatly acids
e) Biosynthesis of Fatty acids
f) Metabolism of Phospholipids and triacylgycerols
g) Metabolism of Ketone bodics
h) Metabolism of Cholesterol
i) Lipo Proteins ? Metabolism and Disorders
j) Lipotropic factors
k) Chemistry and metabolism of Prostaglandins.
14 Hemoglobin structure, Functions and Metabolism, 4
Porphyrias and Hemoglo Binopathies Catabolism of home
15 Integration of Metabolism
2
Metabolic integration; liver, adipose tissue,
Skeletal Muscle and Brain
54
16. Mineral Metabolism
4
Sodium, Patassium, Calcium, Phosphorus, Magnesium,
Manganese, Sulphur, Iron, Copper, Zinc, Iodine,
Cobalt, Fluorine, Selenium and chromium.
17. Nutrition :
4
a) Calorific Value
b) Specific Dynamic Action
c) Energy Requirements
d) Balance Diet, Nitrogen balance, Dietary fiber
e) Foodfads
f) Nutritional disorders kwashiorkor and marasmus
18. Detoxification:
2
19. Hormones :
5
i)
General Principles of Hormonal action
ii)
Outline of Hormone Structure
ii ) Mechanism of Action and metabolic roles of
a) Pituitary
b) Pancreas
c) Adrenal
d) Gonadal
e) Thyroid
20. Functional Tests :
4
a)
Renal
b)
Hepatic
c)
Pancreatic
d)
Gastric
21. Fluid- Electrolyte and Acid - Base Balance
5
22. Plasma Proteins & Immunoglobulins
3
23. Biological Membranse
2
24. Carcinogenesis Malignancy and cell cycle
2
Division of syllabus for university exam
PAPER-I
PAPER-II
1. Enzymes
1. Protein Chemistry and Metabolism
2. Biological Oxidation
2. Mineral metabolism
3. Digestion and absorption
3. Nucleic acid chemistry and
4. Detoxification
metabolism
5. Carbohydrate Chemistry and
4. Genetics
Metabolism
5. Hormones
6. Vitamins
6. Functional tests
7. Nutrition
7. Plasma Proteins and
8. Lipid Chemistry and Metabolism
Immunoglobulins
9. Hemoglobin Structure, functions &
8. Biological membranes
metabolism, Porphyrias and
9. Carcinogens
Hemoglobinopathies
10. Acid-base balance and water ?
Electrolyte balance
55
PRACTICALS IN BIOCHEMISTRY:
40 Hrs.
A. Qualitative :
1.Reactions of Carbohydrates
No.of Practicals
a) Glucose & Fructose
1
b) lactose, Maltose and sucrose
1
c) Identification of Carbohydrates
1
2.Reactions of Proteins :
a) Precipitation reactions
1
b) General colour reactions of Proteins and
c) Albumin and above a&b
1
d) Casein
1
e) Gelatin and peptone
1
f) Identification
2
3.Normal Constitutents of Urine
2
4.Abnormal Constituents of Urine
2
Identification of Abnormal Constituents of urine
2
B. Quantitative :
1.
Blood glucose
1
2.
Blood Urea
1
3.
S. Proteins
1
4.
Urinary Creatinine
1
5.
CSF Analysis
1
a) Proteins (I) Sulphosalicylic acid test (ii) Pandy's test
b) Glucose
c) Chlorides
10 Hrs.
C. Demonstrations :
1. Chromatography
2. Electrophoresis
3. GTT
4. S. Uric acid estimation
5. S. Bilirubin estimation
Revision and conduct of Tests
= 5 Practicals
20 Hrs.
Tutorials and group discussions = 10 Practicals 50 Hrs.
NOTE: Each Practical Carries two hours.
56
RECOMMENDED BOOKS :
1. Review of Biochemistry _ Harper
2. Biochemistry by Debajyoti das
3. Text book of Biochemistry for Medical Students by D.M. Vasudevan & Sreekumari
4. Text book of Medical Biochemistry by M.N.Chatterjea and Rana shinde
5. Medical Biochemistry by Dinesh Puri
REFERENCE BOOKS
1. Biochemistry
Lehninger
2. Biochemistry
Stryer
3. Text Book of Clinical Biochemistry
Tietz
4. Clinical Biochemistry
Varley
57
This post was last modified on 28 June 2021