[AHS 0321] MARCH 2021 Sub. Code: 5016
(AUGUST 2020 EXAM SESSION)
B.Sc. MEDICAL LABORATORY TECHNOLOGY
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SECOND YEAR (From 2010-2011 onwards)PAPER I – HISTOPATHOLOGY AND CYTOLOGY TECHNIQUES
Q.P. Code : 725016
Time: Three hours Answer ALL Questions Maximum: 100 Marks
I. Elaborate on: (3 x 10 = 30)
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- What is tissue processing? Describe in detail about different stages of Tissue processing.
- Enumerate the cytological stains. Describe in detail about the composition, preparation, staining procedure and advantages of May Grunwald Giemsa staining.
- What is PAS? Explain the principle, procedure and applications of PAS staining.
II. Write notes on: (8 x 5 = 40)
- Define and Classify Fixative.
- What is Microtome? Write about Rotary Microtome.
- Enumerate amyloid stains and write the procedure of congo red staining.
- How to prepare cell block and mention about its application.
- What is FNAC? Write about the advantages and disadvantages.
- Reticulin staining.
- Honing and stropping.
- Museum techniques.
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III. Short answers on: (10 x 3 = 30)
- Types of Microtome Knives.
- Enumerate 3 Fat stains.
- What is Decalcification? Write any 2 decalcifying fluids.
- What is mounting medium? Write any 2 different mounting medias.
- Enumerate the Types of Microscope.
- Spray fixative.
- Write the composition of PAP Stain.
- What is Antigen retrieval.
- Uses of Water bath in histopathology.
- Write briefly on Mordant.
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