Printed Pages: 4
277
NBT-303
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(Following Paper ID and Roll No. to be filled in your Answer Book)
Paper ID: 154303 Roll No.
B. TECH.
(SEM. III) THEORY EXAMINATION, 2015-16
BIOCHEMISTRY
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[Time:3 hours] [Maximum Marks:100]
Section-A
Note: Attempt all parts. All parts carry equal marks. Write answer of each part in short.
- Put True (T) or False (F) in the space : (2x10=20)
- The nucleosome consists of core of eight sub-units of protein called histones containing DNA. ()
- The naturally occurring small peptide hormone secreted by pituitary gland is called insulin. ()
- The electrophoresis in presence of SDS separates the protein on the basis of charge. ()
- The amino terminal residue in a polypeptide chain can be determined by 1, fluoro, 2, 4 dinitrobenzene ()
- Myoglobin was the first protein for which the three dimensional structure was worked out by X-ray crystallography. ()
- Ceruloplasmin is a protein which transports zinc. ()
- Hypoxanthine is derived from adenine by replacement of its amino group with methoxy group. ()
- Pyrimidines and purines which contain -NH2 group are weakly basic. ()
- ß-structure is one of the most important secondary structures in protein. ()
- Base composition of DNA varies from species to species. ()
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Section-B
Attempt any five questions from this section : (10 x 5 = 50)
- Explain the principle of Capillary Electrophoresis.
- How is Ramchandran Plot used in determining Secondary structure of proteins ?
- How glucokinase and insulin regulate blood glucose levels?
- What is Henderson Hasselbach equation?
- Explain the differences of denaturation of protein by urea and guanidine hydro chloride.
- Define the reasons for Wobble effect.
- Cation-exchange resins be used for purification of protein.
- What do you understand by homologous proteins. Explain giving suitable examples.
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Section-C
Attempt any two questions from this section. (15x2=30)
- Describe hemoglobin structure and its role in oxygen transport and release.
- Describe two examples of diseases caused by altered protein conformation.
- Explain De novo salvage pathways of pyrimidine nucleotides.
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