Download B.Pharm 3rd Year 2017 August 564266 Pharmaceutical Biotechnology Question Paper

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(LL 4266) AUGUST 2017 Sub. Code: 4266

B.PHARM. DEGREE EXAMINATION

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(Common to Regulations 2004 — IV year candidates)

THIRD YEAR

PAPER VI - PHARMACEUTICAL BIOTECHNOLOGY

O.P. Code: 564266

Time: Three hours Maximum: 100 Marks

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1. I. Elaborate on: (2x20=40)

   1. Explain the production of Insulin by rDNA technology.
   2. Discuss the methods of transfer of genetic materials in bacteria.
2. 1. Explain the design of Fermentor.

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   2. Discuss microbiological assay of Antibiotics.

3. II. Write notes on: (8x5=40)

   1. Types of culture media.
   2. Filtration sterilization.

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   3. Chick Martin Test.
   4. Preparation of BCG vaccine.
   5. Differentiate between gram positive and gram negative bacteria.
   6. Production of citric acid by Fermentation method.
   7. Southern blotting technique.

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   8. Production of single cell protein.

4. III. Short answers on: (10x2=20)

   1. Define Phototrophs and Chemotrophs.
   2. Classify bacteria based on temperature.

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   3. Active immunity.
   4. Haptens.
   5. Microorganisms used in production of alcohol.
   6. Component of Biosensor.
   7. Plasmid vector.

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   8. Define sterilization.
   9. Types of sparger.
   10. Culture media for animal cells.

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