Download B.Pharm 4th Year 2012 August 564270 Modern Methods of Pharmaceutical Analysis Question Paper

(LB 4270)

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AUGUST 2012

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Sub. Code: 4270

FOURTH YEAR B.PHARM. EXAM

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PAPER IV ? MODERN METHODS OF PHARMACEUTICAL ANALYSIS

Q.P. Code: 564270

Time: Three Hours

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Maximum: 100 marks

(180 Min) Answer ALL questions in the same orde

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I. Elaborate on:

Pages Time Marks

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(Max.)(Max.)(Max.)



1. (a) Define Chemical Shift. Explain the factors affecting

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chemical shift.

(b) Write the principle, working procedure, types and
applications of Radio Immuno Assay.

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19

33

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20

2. (a) Write the theory, apparatus, working and applications

of Polarography.

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(b) Define electrophoresis. Explain in detail about Gel
electrophoresis and Capillary electrophoresis.

19

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33

20

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II. Write notes on:

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1. Write a note on ultracentrifugation.

3

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8

5

2. Explain the types of columns used in High Performance

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Liquid Chromatography.

3

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8

5

3. What are the types of ion exchange resins used in ion

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exchange chromatography? Explain.

3

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8

5

4. Write the identification and quantification procedures

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involved in the separation of individual components

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from mixture by Thin Layer

Chromatography.

3 8 5

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5. Write the principle involved in the separation of amino

acids by Paper Chromatography. Explain the reaction of
amino acid with Ninhydrin reagent.

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3

8

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5

6. Write the principle, instrumentation and applications of

Nephalometry.

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3 8 5

7. Explain the sampling techniques for solids in IR

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Spectrophotometry.

3 8 5

8. How will you calculate max by Woodward ?Fischer rule?3

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8

5

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III. Short Answers:

1. Bathochromic shift and Hypsochromic

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shift.

1 5 2

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2. Relationship between concentration and fluorescence

intensity.

1 5 2

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3. Hallow Cathode Lamp.

1

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5

2

4. Reverse Phase Chromatography.

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1

5

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2

5. Junction

potential.

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1 5 2

6. Equivalent Conductance and Molecular

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conductance. 1 5 2

7. Spin ? Spin Coupling.

1

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5

2

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8. Molecular ion peak.

1

5

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2

9. Capacity facto

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1 5 2

10. Nernst ? Glowe

1

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5

2

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