AUGUST 2012
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Sub. Code: 4270
FOURTH YEAR B.PHARM. EXAM
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PAPER IV ? MODERN METHODS OF PHARMACEUTICAL ANALYSISQ.P. Code: 564270
Time: Three Hours
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Maximum: 100 marks
(180 Min) Answer ALL questions in the same orde
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I. Elaborate on:
Pages Time Marks
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(Max.)(Max.)(Max.)1. (a) Define Chemical Shift. Explain the factors affecting
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chemical shift.
(b) Write the principle, working procedure, types and
applications of Radio Immuno Assay.
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19
33
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202. (a) Write the theory, apparatus, working and applications
of Polarography.
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(b) Define electrophoresis. Explain in detail about Gel
electrophoresis and Capillary electrophoresis.
19
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33
20
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II. Write notes on:
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1. Write a note on ultracentrifugation.
3
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85
2. Explain the types of columns used in High Performance
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Liquid Chromatography.
3
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85
3. What are the types of ion exchange resins used in ion
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exchange chromatography? Explain.
3
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85
4. Write the identification and quantification procedures
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involved in the separation of individual components
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from mixture by Thin LayerChromatography.
3 8 5
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5. Write the principle involved in the separation of amino
acids by Paper Chromatography. Explain the reaction of
amino acid with Ninhydrin reagent.
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3
8
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56. Write the principle, instrumentation and applications of
Nephalometry.
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3 8 5
7. Explain the sampling techniques for solids in IR
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Spectrophotometry.3 8 5
8. How will you calculate max by Woodward ?Fischer rule?3
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8
5
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III. Short Answers:
1. Bathochromic shift and Hypsochromic
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shift.
1 5 2
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2. Relationship between concentration and fluorescenceintensity.
1 5 2
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3. Hallow Cathode Lamp.
1
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52
4. Reverse Phase Chromatography.
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1
5
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25. Junction
potential.
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1 5 2
6. Equivalent Conductance and Molecular
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conductance. 1 5 27. Spin ? Spin Coupling.
1
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5
2
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8. Molecular ion peak.1
5
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2
9. Capacity facto
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1 5 210. Nernst ? Glowe
1
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5
2
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