Download The West Bengal University Of Health Sciences (WBUHS) 2nd Year MBBS Microbiology Syllabus
MICROBIOLOGY
A. Written Paper: Two Papers, (40+40=80), 2hrs.each paper.
.Paper I -General Bacteriology, Systemic Bacteriology, Immunology.
Paper II ?Virology, Mycology, Parasitology.
The four questions in each theory paper will preferably have the following distribution of
mark.
Full marks-40, Time-2 hrs..
Q.l. One (out of two) Clinical problem oriented question consisting of 2-4 small
segments. Marks for each segment will be indicated separately. =10
Q.2. Three short note type questions (out of four) 4x3=12
Q.3. Three (out of four) short answer type/explanation of statement/difference
between/mechanism of action/comment on 4x3=12
Q 4. Three short answer type questions 2x3=6
Answer to each question should be given by the candidates in a separate answer book.
Only one examiner will examine all the answer scripts to the same question in that
center.
B. OraI /Viva
i) General Bacteriology, Immunology, Systemic Bacteriology
-9 marks
ii) Virology, Mycology, Parasitology
-6 marks
C. Practical- 25 marks. Time 1.1/2 hr. + 1/2h hr for spotting = 2 hrs.
O
Identification of unknown bacterial culture
-8
O
Ziehl-Neelsen Staining of Sputum smear supplied
-3
O
Microscopical examination of supplied stool smear
-3
O
A serological test by common slide agglutination method
3
O
Laboratory Note Book
-3
O
Spotting
-5
ASSESSMENT CARD
( TO BE KEPT IN THE DEPARTMENT)
Full Marks ? Viva voce ? 10 X 20 = 200, Practical = 20 X 3 = 60.
Name of student :
Batch :
Roll
No :
Sl.
Topics
Oral
Marks Obtained
No.
3rd semester
Practical
Signature of
Teacher
1.
History, Classification, Morphology &
Physiology of Bacterial genetics.
2.
Sterilization, methods of isolation &
identification.
3.
Gram positive cocci
4.
Gram negative cocci, corynebacteria
5.
Mycobacteria
4th semester
6.
Spore bearers
7.
Enterobacteriaceae
8.
Vibrios, Pseudomonas & Pravobacteria.
9.
Spirillum, Actinomycetes, Campylobacter.
10.
Antigen, Immunoglobulin, Complement.
11.
Immunity & hypersensitivity.
12.
Immunodeficiency states & immunological
reactions.
5th semester
13.
Spirochetes.
14.
Rickettsiae, Chlamydia, mycoplasma, general
virology.
15.
D.N.A. viruses.
16.
R.N.A. Viruses.
17.
Mycology
18.
Protozoa
19.
Nematodes
20.
Cestodes & trematodes.
N. B. 1. Students must appear for assessment on scheduled dates, failing which no assessment
will be taken on later dates except on special grounds.
Students must keep laboratory note book up to date failing which no student will
be allowed for practical assessment.
Signature of the Head of the Department.
ITEM CARD
Name :
College :
Year :
Roll No.
DISTRIBUTION OF INTERNAL ASSESSMENT MARKS
THEROTICAL DAY TO DAY ASSESSMENT
GENERAL
SYSTEMIC
PROTOZO HELMINT
IMMUNO
VIRO
MYCO TOTAL
10%
BACTERI
BACTERI
OLOGY
HOLOGY
LOGY
LOGY
LOGY
OF 75
OLOGY
OLOGY
10
10 10
10
10
10
10
5
75
7.5
PRACTICAL DAY TO DAY ASSESMENT
Microscope
Culture Grams' AFB
Stool
Identification Spotting Serology Total
10% of 75
&
media
stain
Stain
Exam.
of unknown
Sterilization
Culture
10
10
10
10
10
10
10
5
75
7.5
SENT UP EXAMINATION ? THEROTICAL
TOTAL THEORITICAL
Theory
Oral
Total
Total
10% of
(1) Day to day Assessment Total
40x2=80
20
Theory + Theory + Oral
Theory +
Theoretical-7.5
(1+2)
Oral
in 75
Oral
(2) Sent up Exam.
7.5+7.5=15
Theory + Oral = 7.5
PRACTICAL
TOTAL PRACTICAL
Internal
10% of
Day to day
Practical
Sent up Exam. Total ( 1+2)
Assessment
Practical (7.5)
assessment
Day to day
Pr. 7.5
7.5+7.5=15
Practical 25
Practical 7.5
7.5
(calculated in
75, i.e. 25 X 3)
CURRICULUM & SYLABUS FOR THE MBBS COURSE OF STUDIES
A Duration
: 1.5 yrs. 3rd, 4th & 5th Semester
B. Total hours of Teaching
: 250 hrs. Comprising of
1) Lecture + Lecture demonstration =
100 x 1 hr
= 100 hrs
2) Practical class
50 x 2 hrs
= 100 hrs
3) Tutorials
25 x 2 hrs.
= 50 hrs.
_____________________________
TOTAL
=250 hrs
C. Curriculum (Syllabus)
Topic for theoretical Class
1. THEORY
No.
Topic
Class
hrs.
1.
Introduction to Microbiology. History and
Classification.
One
Genera1 Bacteriology
2.
Morphology of Bacteria & Methods of study
of Morphology.
Two
3.
Physiology of Bacteria, Metabolism &
products thereof
One
4.
Growth requirements of Bacteria,
Growth Curve/measurement of growth
One
5.
Sterilization & disinfection
One
6.
Host-parasite relationship
One
7.
Bacterial genetics with variation
One
8.
Antimicrobial agents, mechanism of action,
Mechanisms of bacterial drug resistance and Sensitivity Testing.
IMMUNOLOGY
1.
Introduction to Immunology. Natural &
Non-specific Immune Mechanisms
One
2.
Antigen, Hapten, Adjuvants
One
3.
Antibody
One
4.
Complement System
One
5.
Structure & Function of Immune System
Two
6.
Immune response with T -B Cell Co-operation
One
7.
Cytokines with its role in cell mediated
Immune response
One
8.
Hypersensitivity and related disorders
Two
9.
Antigen -antibody reactions methodology
of testing .
Two
10.
Immune deficiency disorders and autoimmune Diseases
One
11.
Vaccine and scope of Immunotherapy
One
Pathogenic Bacteria and Diseases
1.
Methods of study of bacteria
One
2.
Staphylococcus: Diseases produced, modes of
transmission, pathogenesis & diagnosis.
One
3.
Streptococcus: diseases, transmission, pathogenesis, diagnosis
Streptopneumonae:
epidemiology.
Two
4.
Neisseria: Important species, diseases caused
:
Pathogenesis, diagnosis, Epidemiology
5.
Corynebacterium diphtherae: pathogenesis, transmission, diagnosis, Vaccine.
6.
Listeria, Erysepalothrix, Legionella, etc.
One
Diseases caused, diagnosis.
7.
Mycobacterium tuberculosis -Transmission, Pathogenesis, types, immunity
Hypersensitivity, interpretation of Results of Mantoux text diagnosis, Vaccine- Two
Leprosy--transmission, features, types diagnosis etc., Role of vaccine
Atypical Mycobacteria.
Classification, diseases, diagnosis
8. Actinomyces &
: Disease caused, mode of transmission,
Nocardia
Diagnosis
Aerobic spore-Bearers: Bacillus. Important species, disease caused.
Pathogenesis. diagnosis, epidemiology of Anthrax.
One
9. Nonsporing
Bacteroides Sp. etc. : Diseases produced,
anaerobes
features, diagnosis.
10. Anaerobic
Clostridia- Tetanus, Gas-gangrene, Food poisoning,
Spore bearers Botulism : Pathogenesis, infection, transmission,
Diagnosis, treatment and prophylaxis.
Three
11. Enterobactericeae: Diseases caused by E.coli, Klebsiella, Enterobacter etc.
Two
12. Enteric fever and Salmonella sp: Food poisoning, Pathogenesis, Diagnosis.
Two
13
Shigellosis & Acute Bacillary dysentery
One
14. UTI and other diseases of proteus sp. Providencia etc.
One
15
Yersinia sp.
- Plague ? Pathogenesis Types, diagnosis,
epidemiology, food poisoning
One
16.
Vibrios -
Important species, Cholera -pathogenesis, transmission,
17. Campylobacter & Helicobacter -Diseases caused, pathogenesis, diagnosis.
One
18.
Pseudomonadeceae
Importance, pathogenesis, diagnosis
One
19.
Haemophilus: Disease, pathogenesis diagnosis
One
20.
Bordetella sp : Disease caused, transmission, pathogenesis, diagnosis
One
21.
Brucella sp.: Disease caused, transmission, pathogenesis. diagnosis.
One
22.
Miscellaneous bacteria like
Pasteurella, francisella,
: Disease caused
Streptobacillus, spirilium etc. epidemiology
One
23.
Spirochetes:
Nonpathogenic spirochetes syphilis
yaws, pintas, bejel, leptospirosis,
Relapsing fevers & lyme disease
Four
24.
Rickettsial disease
Epidemiology & diagnosis
Two
25. Mycoplasma and Chlamydia: diseases including diagnosis.
Two
26. Normal flora of Human body.
One
VIROLOGY
1.
Introduction to virology, general properties of viruses and
Classification of viruses
One
2
Replication of viruses, Antiviral agents
One.
3. Principles of viral diseases
4.
Principles of diagnosis of viral infections
One
5.
Common viral vaccines
One
6.
Bacteriophage
7.
Diseases caused by Herpes viruses, Vericella zoster virus,
CMV EBV etc.
One
8.
Hepatitis viruses, A,B,C,D,E; Hepatitis A & B properties laboratory diagnosis One
9.
Picorna viruses -and diseases produced with special mention to
Pathogenesis of polio diagnosis and prevention.
One
10.
Viral gastroenteritis ?agents, pathogenesis, diagnosis.
One
11.
Rhabdo viruses -General character of Rabies virus, pathogenesis of
disease diagnosis prophylaxis.
One
12.
Orthomyxo and paramyxo viral diseases (Influenza,' Mumps, Measles
Rubella) including vaccines.
One
13(a) Retrovirus -HIV infection & AIDS & other retrovirus;
(b) Oncoviruses -examples & properties & mechanisms of viral
One
etiology of tumor scope of immunotherapy.
14(a) Arboviruses and arboviral diseases prevalent in India: epidemiology & diagnosis
(b) Slow viral diseases ?etiology, diagnosis
One
MYCOLOGY
1.
Introduction, Classification, principles of laboratory diagnosis
One
2.
Superficial mycosis
One
3.
Subcutaneous mycosis
One
4.
Deep mycosis
One
5.
Opportunistic mycosis
One
_______
five
PARASITOLOGY .
1.
Introduction, Classification, definition and types of hosts.
Definition and types of parasites
One
2.
Intestinal amoebiasis and complications -mode of infection
pathogenesis, laboratory diagnosis.
One
3.
Flagellated protozoa -intestinal & genitourinary
One
4.
Haemoflagellates -diseases, life cycle, vector for transmission,
laboratory diagnosis (Trypanosomes, leishmania).
One
5.
Malaria -types, parasite -Morph., life cycle, vector,
Two
laboratory diagnosis.
6.
Toxoplasmosis and other opportunistic protozoa infections.
One
7.
Classification of helminthes and general characters of nematodes,
Three
introduction to intestinal nematodes, strongyloides stercoralis,
Ascaris lumbricoides, Hook worm, Trichinella spiralis, Enterobius
Vermicularis trichiurae life cycle, disease, laboratory. Diagnosis, epidemiology
8.
Filariasis -diseases, vector, life cycle of parasite Pathogenesis
Two
of disease, laboratory diagnosis.
9.
Dracunculosis -life cycle of parasite, mode of infection,
One
epidemiology, laboratory diagnosis.
10.
General characters of cestodes, Taeniasis -hosts, mode of
One
infection, life cycle of parasite infection, laboratory diagnosis.
11.
Echinococcus granulosus-Morphology,1ife cycle of parasite,
One
mode of infection, prevention ,laboratory diagnosis.
12.
D.latum and other cestode infections
One
13.
Trematodes -classification, diseases caused,. Life cycle of
One
schistosomes and general principles of laboratory diagnosis
________________
Nineteen
II. PRACTICAL:
1.
Parts and use of microscope and microscopy
1
2.
Instruments and glass wares used in Microbiology
1
3.
Universal presence of microbes
1
4.
Commonly used media and culture techniques
2
(Media -simple basal media -liquid, solid, enriched media,
selective media, enrichment media, Indicator Media)
Transport Media, Blood culture media, sugar media,
Anaerobic media Name, type, composition, sterilization and use.
5.
Sterilization methods used for different purpose-
1
basic principles, instruments/chemical agents used
6.
Study of morphology of bacteria :
a) Gram staining
2
b) Albert staining
1
c) Ziehl-Neelsen staining
2
7.
Study of motility of bacteria by
2
a) Hanging drop method
d) Capillary tube method.
b) Cragie's tube method
e) Dark-ground microscopy
c) Straight loop inoculation method
8.
Methods of antimicrobial sensitivity testing
1
a) Disk diffusion
(b) Tube dilution
9.
Study of Staphylococcus aureus and staph. epidermidis.
2
Colony morphology. Pigment production. Gram stain.
Motility, Coagulase and otber confirmatory tests including
Catalase test.
10.
Study of -Gram + cocci
1
a) Haemolytic properties of Staph., Strepto., Pneumococci
b) Gram staining, Morphology, Study of Strepto, Staphylo
Neisseria, Pneumococcus, Clostridia.
11.
Corynaebacterium -
Albert Stain
2
Media used
12.
Mycobacterium -
Z -N Stain
3
Study of charts
Confirmatory diagnosis of
Tuberculosis & Leprosy
D/D Myco. tuberculosis & M. leprae in smear.
13.
Study of spores -Gram stain, Spore-Stain (Carbol Fuchsin)
1
14.
Study of Stained Smear, Capsule ?India Ink staining (Negative -
Stain) Carbol Fuchsin (Positive stain), Methods of Anarobiasis.
15.
Enterobacteriace
(a) E.coli
1
(Use of media)
Colony character
Biochemical reactions for
Identification of the bact. &
Final jdentificajon with antibiogram)
(b ) Klebsiella sp.
1
(c) Proteus sp.
1
(d) Salmonella sp.
1
(e) Shigella sp
1
16.
Vibrio -Gram Stain Motility test Oxidase Biochemical
1
Reactions.
17.
Pseudomonas sp. -Gram Stain. Motility test, Oxidase
1
18.
Serological Tests: VDRL Test RPR
3
Agglutination -Widal, Latex Agglutination test, ELISA -any common test done.
19.
Introduction to Parasitology - Types of clinical
2
materials different types of tests done.
Steps of exam. of Stool Smear
Steps of exam. of Blood Smear
Steps of exam. of marrow Smear.
20.
Blood Parasites -
Malaria Parasite
1
L.D.Body
1
Microfilaria
1
21.
Adult Parasites -
Nematodes
2
Cestodes
2
Trematodes
1
22.
Examination of Stool for ova, parasite & Cyst
3
Saline and Iodine preparations.
23.
Demonstration of fungus by KOH prepn./ lactophenol cotton
1
blue staining.
24.
Demonstration of yeast cells in Gram stains & culture
1
_______________
50
III.
Tutorials ? 25 x2 hrs
=50 hrs
A.
Interpretation of laboratory investigation for diagnosis of Infectious disease and correlation
between clinical features with aetiological agents to be taken up in the form of charts on
diseases of national importance e.g.
a) Tuberculosis
14 x 2 Hrs. = 28
b) Leprosy
c) Cholera
d) Enteric fever
e) Diphtheria
f).Whooping coughs
g) Tetanus
h) Malaria
i) Kala-azar
j) Filaria
k) Dengue
t) Hepatitis B
m) AIDS
n) Hookworm anaemia
B.
Clinical Microbiology:
11 x 2 hrs.= 22 hrs
1.
Upper respiratory tract. Infections with lab diagnosis
2.
Lower respiratory tract infections with lab diag.
3
Bacterial food poisoning with lab. diag.
4
Terminology: gastroenteritis, diarrhoea, dysentery, pseudo membranous colitis
diarrhoea and its lab. diag.
5.
Dysentery and its lab. diag.
6.
Meningitis -types, agents and its lab. diag
7
Terminology of Bacteraemia, Septicaemia, pyaemia and its lab. Diagnosis/ PUO
(Blood culture)
8
Urinary tract Infection, organism and its lab. diag.
9.
Sexually transmitted diseases list and lab. diag.
10
Hospital acquired infection and its control
11.
Bacteriology of milk, water air.
Model Question in Microbiology
2nd Professional MBBS
MICROBIOLOGY
Time :- 2 hours
First -Paper
Full
Marks: 40
Q1. A 8 year old girl was admitted through emergency because of high fever and limping gait. Her mother states that
she developed these symptoms after a bout of sore throat accompanied by high fever three weeks back.
What may be the probable diagnosis? How do you proceed in the microbiological laboratory for finding its aetiological
agents? What serological tests do you suggest in this case? 1 +6+ 3 = 10
Or
A 24 years old person was admitted through emergency because of severe dehydration with I sunken eyes following a
bout of rice watery stool accompanied with vomiting.
What is this condition?
What are the aetiological agents responsible for this situation?
How do you confirm anyone of the aetiological agents in the laboratory?
1 + 3+6 = 10
Q2. Write short notes on the following (any three)
3 x 4 =12
i)
Fimbria and its clinical significance.
ii)
Bacterial capsule.
iii)
Weil Felix test.
iv)
Environmental Mycobacteria.
v)
Pyoderma gangrenosum
Q3. Comments on (any three)
3 x 4 = 12
i)
A positive mantoux test in an adult has many fallacies.
ii)
Antibiogram is must for staphylococcus because of MRSA.
iii)
The presence of morphologically similar organisms does not prove the case to be of diphtheria.
iv)
The presence of acid fast bacilli in sputum smear should be reported in exact or
approximate number because of prognostic value.
v)
Apart form pyogenic lesion streptococci may be related to Non pyogenic lesions with grave
consequences.
Q4. Differentiate between
3 x 2 = 6
i)
Gram positive and gram negative cell wall.
ii)
Active and passive immunity.
iii)
IgG and IgM.
Second Paper
Time :- 2 hours
Full Marks: 40
Q1.
A twenty five year old male came to you with history of fever and yellow colouration of urine
which developed within two to three days. On examination his abdominal examination is quite
normal expect tenderness and slight soft enlargement of liver.
What is your diagnosis? .
What are the tests that you will do in microbiological lab to confirm the aetiology?
If the icterus or the symptoms persist beyond six months, what are the serological parameters you
will ask for?
1 +6+ 3 = 10
Or
An emaciated young person comes to you with history of fever for three months and pain in the
left side of abdomen. On examination he has a huge hepatosplenomegaly with severe anemia.
What may be the condition?
If it is a parasitological disease how do you go for diagnosis in laboratory?
What are the serological tests done for this condition?
1+6+3 = 10
Q2.
Write short notes on (any three)
3 x 4 = 12
i)
Prion mediated diseases.
ii)
Neurological vaccines of Rabies. iii) CD4 and CD8 counts for HIV.
iv)
Congenital defects associated with viruses.
v)
Infective forms of Giardia lamblia, Ascaris lumbricoides, Enterobius verimicularis.
Q3.
Comment on (any three)
3 x 4 = 12
i)
Neurological vaccines against Rabies have many problems.
ii)
There are many vaccines against Hepatitis viruses used presently.
iii)
The floatation concentration technique may be used for ova, cysts etc. iv) The filarial infections
can be detected in blood even in daytime.
v)
The asexual spores of fungi can be used for diagnosis in superficial dermatological infection.
Q4.
Differentiate between
3 x 2 = 6
i)
Superficial and subcutaneous dermatophytes.
ii)
Virus and Viroids.
iii)
Antigenic shift and antigenic drift in influenza viruses.
This post was last modified on 29 June 2021