--- Content provided by FirstRanker.com ---forms greyish brown to black precipitate of
Principle
lead sulphide (PbS).
? Test tube
? Test tube holder
?
Burner
aratus
? 20% sodium hydroxide
? 2% lead acetate
Reagents
NaOH and 0.5ml of 2% lead acetate.A white
precipitate is obtained due to denaturation of
proteins.now boil the mixture,the white ppt
ns ocedu
greyish br re
own or black indicating the
presence of sulphur.
? A positive reaction is due to the presence of a
sulphur containing amino acids in the protein.
? This can be Cysteine or Methionine
Principle
lead sulphide (PbS).
--- Content provided by FirstRanker.com ---
? Pipette? Test tube
? Test tube holder
?
--- Content provided by FirstRanker.com ---
AppBurner
aratus
--- Content provided by FirstRanker.com ---
? Original solution or protein solution? 20% sodium hydroxide
? 2% lead acetate
Reagents
--- Content provided by FirstRanker.com ---
? To 1ml of O.S in a test tube add 1ml of 20%NaOH and 0.5ml of 2% lead acetate.A white
precipitate is obtained due to denaturation of
proteins.now boil the mixture,the white ppt
--- Content provided by FirstRanker.com ---
tur Prns ocedu
greyish br re
--- Content provided by FirstRanker.com ---
own or black indicating the
presence of sulphur.
? A positive reaction is due to the presence of a
--- Content provided by FirstRanker.com ---
sulphur containing amino acids in the protein.
? This can be Cysteine or Methionine
--- Content provided by FirstRanker.com ---
Interpretation