Download MBBS Practicals Biochemistry Determination of Blood Glucose

Download MBBS (Bachelor of Medicine and Bachelor of Surgery) Practicals Latest Biochemistry Determination of Blood Glucose

Folin & Wu method

Reagents
10% sodium tungstate

for preparing

2/3 N H2SO4 Plasma free filtrate

Alkaline Copper Sulphate solution
Phosphomolybdic acid
Standard glucose solution(.2mg/2ml)


Folin-Wu test tube

This is special type of test tube .
The constricted part of the tube prevents

the entry of O2 to the bulbar portion of

test tube and thus prevents the re
oxidation of cuprous oxide present in the
bulb of tube.


Principle
Glucose and other reducing substances reduce cupric to

cupric oxide, which on reaction with the phosphomolybdic
acid produces a blue color. The blue color is produced due
to reduction of molybdate ions.

The intensity of color is directly proportional to amount of

reducing substance which is to be determined.
Protein free filtrate(PFF)

Protein free filtrate is prepared by

taking 2ml of oxalated blood.

Add 14ml distal water
2ml of 10% sodium tungstate.
2ml of 2/3 N H2SO4
Shake well,
keep at room temperature for 10-

15min until color change to brown

red then filter it clear fluid is PFF.

2ml of blood is diluted 10 time, so

in calculation we add dilution

factor 10.
Procedure
Label 3 folin wu test tubes as
Unknown(U), standard (S), and blank(B).
Calculation

Blood glucose = 25 ? .2 /22 ? 10/2 ?100

= 113.6 mg/dl
GLUCOSE TOLERANCE TEST

Dr Gulnaz Begum
GLUCOSE TOLERANCE TEST

This test is used to determine the ability of body to

tolerate or utilize glucose in circulation.

It can measure
Active insulin
Ability of liver to absorb or store excessive amount of

glucose.

Effectiveness of GIT to absorb glucose.


Significance of GTT


Contraindications of GTT


Types of GTT

in

Patients having the error in absorption from GIT, due to

malabsorption syndrome, steatorrhea and caeliac disease.






Normal GTT