for preparing
2/3 N H2SO4 Plasma free filtrate
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Alkaline Copper Sulphate solutionPhosphomolybdic acid
Standard glucose solution(.2mg/2ml)
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Folin-Wu test tubeThis is special type of test tube .
The constricted part of the tube prevents
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the entry of O2 to the bulbar portion oftest tube and thus prevents the re
oxidation of cuprous oxide present in the
bulb of tube.
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Principle
Glucose and other reducing substances reduce cupric to
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cupric oxide, which on reaction with the phosphomolybdicacid produces a blue color. The blue color is produced due
to reduction of molybdate ions.
The intensity of color is directly proportional to amount of
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reducing substance which is to be determined.
Protein free filtrate(PFF)
Protein free filtrate is prepared by
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taking 2ml of oxalated blood.
Add 14ml distal water
2ml of 10% sodium tungstate.
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2ml of 2/3 N H2SO4Shake well,
keep at room temperature for 10-
15min until color change to brown
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red then filter it clear fluid is PFF.
2ml of blood is diluted 10 time, so
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in calculation we add dilutionfactor 10.
Procedure
Label 3 folin wu test tubes as
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Unknown(U), standard (S), and blank(B).Calculation
Blood glucose = 25 ? .2 /22 ? 10/2 ?100
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= 113.6 mg/dlGLUCOSE TOLERANCE TEST
Dr Gulnaz Begum
GLUCOSE TOLERANCE TEST
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This test is used to determine the ability of body to
tolerate or utilize glucose in circulation.
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It can measureActive insulin
Ability of liver to absorb or store excessive amount of
glucose.
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Effectiveness of GIT to absorb glucose.
Significance of GTT
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Contraindications of GTT
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Types of GTTin
Patients having the error in absorption from GIT, due to
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malabsorption syndrome, steatorrhea and caeliac disease.
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Normal GTT
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