FINAL EXAM
NATIONAL BOARD OF EXAMINATIONS
JUNE 2020
BIOCHEMISTRY
PAPER-IV
BCHEM/J/20/03/IV
Max. Marks:100
Important Instructions:
Attempt al questions in order.
Read the question careful y and answer to the point neatly and legibly.
Do not leave any blank pages between two answers.
Indicate the question number correctly for the answer in the margin space.
Answer all the parts of a single question together.
Draw table/diagrams/flowcharts wherever appropriate.
Write short notes on:
1. Describe the stages of development of vaccine against viral diseases and
7+3
(SARS-CoV-2).
2. List the antiviral drugs used in COVID-19 and write their mechanism of
3+7
action.
10
SARS-CoV-2 virus by genetic engineering techniques.
4. a) Two dimensional gel electrophoresis
5+5
5. a) Multiplex PCR.
5+5
b) Ct value and absolute count of virus by RT-PCR.
6. Describe the differences between:
a) Chemiluminiscence and Bioluminiscence
b) Turbidimetry and Nephelometry
7. Describe the use of mass spectrometry in analyzing the amino acid, protein
7+3
8. Write basic principle, instrumentation requirement and clinical application of 5+2+3
Fluorescent in situ hybridization (FISH) technique.
9. Describe the techniques used in characterization of a novel protein.
10
analysis and bioinformatics tool in biochemistry diagnostic research.
************
POSSESSION/USE OF CELL PHONES OR ANY SUCH ELECTRONIC GADGETS IS NOT PERMITTED INSIDE THE
EXAMINATION HALL.
NATIONAL BOARD OF EXAMINATIONS
JUNE 2020
BIOCHEMISTRY
PAPER-IV
--- Content provided by FirstRanker.com ---
Time: 3 hoursBCHEM/J/20/03/IV
Max. Marks:100
Important Instructions:
Attempt al questions in order.
--- Content provided by FirstRanker.com ---
Each question carries 10 marks.Read the question careful y and answer to the point neatly and legibly.
Do not leave any blank pages between two answers.
Indicate the question number correctly for the answer in the margin space.
Answer all the parts of a single question together.
--- Content provided by FirstRanker.com ---
Start the answer to a question on a fresh page or leave adequate space between two answers.Draw table/diagrams/flowcharts wherever appropriate.
Write short notes on:
1. Describe the stages of development of vaccine against viral diseases and
7+3
--- Content provided by FirstRanker.com ---
challenges in development of vaccine against virus causing COVID-19(SARS-CoV-2).
2. List the antiviral drugs used in COVID-19 and write their mechanism of
3+7
action.
--- Content provided by FirstRanker.com ---
3. Describe the techniques that can be used to produce Spike protein of10
SARS-CoV-2 virus by genetic engineering techniques.
4. a) Two dimensional gel electrophoresis
5+5
--- Content provided by FirstRanker.com ---
b) Role of chromatography in separation of nucleic acids.5. a) Multiplex PCR.
5+5
b) Ct value and absolute count of virus by RT-PCR.
6. Describe the differences between:
--- Content provided by FirstRanker.com ---
5+5a) Chemiluminiscence and Bioluminiscence
b) Turbidimetry and Nephelometry
7. Describe the use of mass spectrometry in analyzing the amino acid, protein
7+3
--- Content provided by FirstRanker.com ---
and metabolomics. Add a short note on MALDI-TOF.8. Write basic principle, instrumentation requirement and clinical application of 5+2+3
Fluorescent in situ hybridization (FISH) technique.
9. Describe the techniques used in characterization of a novel protein.
10
--- Content provided by FirstRanker.com ---
10. Write the potential and present uses of artificial intelligence, large data 3+3+4analysis and bioinformatics tool in biochemistry diagnostic research.
************
POSSESSION/USE OF CELL PHONES OR ANY SUCH ELECTRONIC GADGETS IS NOT PERMITTED INSIDE THE
EXAMINATION HALL.
--- Content provided by FirstRanker.com ---