Download Diplomate of National Board (DNB) 2020 June Biochemistry Paper IV Question Paper
NATIONAL BOARD OF EXAMINATIONS
JUNE 2020
BIOCHEMISTRY
PAPER-IV
Time: 3 hours
BCHEM/J/20/03/IV
Max. Marks:100
Important Instructions:
Attempt al questions in order.
Each question carries 10 marks.
Read the question careful y and answer to the point neatly and legibly.
Do not leave any blank pages between two answers.
Indicate the question number correctly for the answer in the margin space.
Answer all the parts of a single question together.
Start the answer to a question on a fresh page or leave adequate space between two answers.
Draw table/diagrams/flowcharts wherever appropriate.
Write short notes on:
1. Describe the stages of development of vaccine against viral diseases and
7+3
challenges in development of vaccine against virus causing COVID-19
(SARS-CoV-2).
2. List the antiviral drugs used in COVID-19 and write their mechanism of
3+7
action.
3. Describe the techniques that can be used to produce Spike protein of
10
SARS-CoV-2 virus by genetic engineering techniques.
4. a) Two dimensional gel electrophoresis
5+5
b) Role of chromatography in separation of nucleic acids.
5. a) Multiplex PCR.
5+5
b) Ct value and absolute count of virus by RT-PCR.
6. Describe the differences between:
5+5
a) Chemiluminiscence and Bioluminiscence
b) Turbidimetry and Nephelometry
7. Describe the use of mass spectrometry in analyzing the amino acid, protein
7+3
and metabolomics. Add a short note on MALDI-TOF.
8. Write basic principle, instrumentation requirement and clinical application of 5+2+3
Fluorescent in situ hybridization (FISH) technique.
9. Describe the techniques used in characterization of a novel protein.
10
10. Write the potential and present uses of artificial intelligence, large data 3+3+4
analysis and bioinformatics tool in biochemistry diagnostic research.
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POSSESSION/USE OF CELL PHONES OR ANY SUCH ELECTRONIC GADGETS IS NOT PERMITTED INSIDE THE
EXAMINATION HALL.
This post was last modified on 31 July 2021