FINAL EXAM
DECEMBER 2020
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MICROBIOLOGY
PAPER-IV
MICRO/D/20/18/IV
Time: 3 hours
Max. Marks: 100
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Important Instructions:
- You are provided with 5 answer sheet booklets. Each individual answer sheet booklet consists of 10 pages excluding the covering jackets.
- Answers to all the questions must be attempted within these 5 answer sheet booklets which must be later tagged together at the end of the exam.
- No additional supplementary answer sheet booklet will be provided.
- Attempt all questions in order.
- Each question carries 10 marks.
- Read the question carefully and answer to the point neatly and legibly.
- Do not leave any blank pages between two answers.
- Indicate the question number correctly for the answer in the margin space.
- Answer all the parts of a single question together.
- Start the answer to a question on a fresh page or leave adequate space between two answers.
- Draw table/diagrams/flowcharts wherever appropriate.
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Write short notes on:
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Blotting techniques. 10
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a) Pulse Field Gel Electrophoresis. 5+5
b) Restriction Fragment Length Polymorphism.
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Describe principle of Loop Mediated Isothermal Amplification (LAMP). 4+6
Discuss its utility in the diagnosis of parasitic diseases.
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Describe the principle of TrueNat test. Compare the advantages and 4+6
disadvantages of TrueNat test and RT-PCR test in diagnosis of SARS-CoV-2.
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What is CLABSI? Discuss the strategies for prevention of CLABSI. 4+6
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Discuss principle and applications of TaqMan PCR assay. What is Strand (3+3)+4
displacement amplification?
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Automation in Bacteriology. 10
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Vaccines available against COVID-19. 10
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Discuss on surveillance of Health Care Associated Infections (HCAIs), 4+3+3
highlighting the methods and types of surveillance.
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Describe the different biosafety levels. Compare the different Biosafety 5+3+2
cabinets. What is DOP test?
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POSSESSION/USE OF CELL PHONES OR ANY SUCH ELECTRONIC GADGETS IS NOT PERMITTED INSIDE THE
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