under control without any medications. Her
JIPMER QUESTIONS
sisters and mother also have history of
JIPMER May 2016
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increased blood glucose during pregnancy,allQn1.A 50 yr male with symptoms of fatigue
were euglycemic after delivery.What is the
and he has swelling of feet and loss of
enzyme defect?
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sensations in legs and anaemia, He also hasa) Glucokinase
dilatation of ventricle and high cardiac output
b) PFK
state.What is the vitamin deficiency
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c) Aldolaseassociated with this presentation?
d) Enolase
a) Vitamin B1
Qn4.Utilisation of ketone bodies extra hepatic
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b) Vitamin B2tissues is by
c) Vitamin B12
A) HMG CoA lyase
d) Vitamin B3
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B) HMG CoA synthaseC ) 3 hydroxy dehydrogenase
Thiamine (or vitamin B1) deficiency, also
D ) Thiophorase
known as beriberi, has traditionally been
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Qn 5Hormone elevated in a alzheimersdivided into two major types: a "dry" form, in
patient who was found wandering outside for
which features of peripheral neuropathy
24 hours without water and food.that
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predominate, and a "wet" form, in which signsparticular hormone can act on the intra
and symptoms of right-sided heart failure
cellular receptor(normal euglycemic patient)
with normal or high cardiac output are the
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presenting features. A fulminant variant,A)glucagon (act on Membrane receptor)
termed Shoshin beriberi (from the Japanese
B)growth hormone
sho meaning acute damage, and shin meaning
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C)epinephrine (act on membrane receptor)heart), complicating either one of the two
D)Cortisol
types, may occur with severe biventricular
Qn 6.A baby boy 10 month old comes with
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failure, metabolic acidosis, variable cardiacvomiting severe jaundice, hepatomegaly and
output with vascular collapse, peripheral
cyanosis and eventually death This syndrome
features of irritability on starting weaning
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is usually preceded by non-specific symptomswith fruit juice .which of the following
such as generalized fatigue, loss of appetite,
enzymes is defective ?
and abdominal pain
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a) aldolase BQn 2.Not a reversible reaction in ETC
b) Fructokinase
a) succinate dehydrogenase
c) glucose 6 phosphatase
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b) Cyt Bd) Galactose 1 Phosphate Uridyl
c) Cytochrome c Oxidase
Transferase
d) NADH reductase
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Qn7Version1).What happens to the /Which?keto acid receives amino group of alanine
released from muscle after exercise before
getting converted to glucose?
a) Oxaloacetate
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b) Malatec) Alpha Keto Glutarate
d) ?
Qn3.A 27 year lady developed severe
Qn7Version 2.What happens to alanine
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hyperglycemia in pregnancy and it returned toreleased from muscle after exercise before
normal after delivery.Her blood sugar is well
getting converted to glucose?
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a) Oxaloacetateb) Malate
c) Alpha Keto Glutarate
Nov 2015
d) ?
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1.SNP without a prior sequence informationis best identified by
Alanine is converted to Pyruvate
a)DNA Microarray
,Pyruvate to Oxaloacetate to Glucose
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b) PCR& Amino group received by Alpha
c) DNA Sequencing
Keto Glutarate to form Glutamate
d)?Fusion gene microarray
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Qn 8.A 60 year old female presents for routineexamination.She is taking fruits vegetables
Using Microarray certain known SNP are
and multivitamin tablets regularly.Her LMP
arranged and unknown labeled
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oligonucleotide carrying or suspectingis 5 years ago.She is very much concerned
polymorphism is added.So a prior information
about the wrinkles around the eyes.Formation
is needed.
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of wrinkles is due to defect inRepeat length Polymorphisms(VNTR) can be
detected by Real time PCR or Amplicon length
a) Fibrillin Synthesis
Analysis.
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b) Collagenasec)
2.FISH used
collagen cross linking
To locate a known genetic loci
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d) Collagen fibrilsQn 9 Why hemolysis occurs in G6pd deficiency
3.Suicidal bag organelle-
?
a) Lysosomes
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a) 6 phospho gulanolactone not formedb) Endoplasmic reticulum
b) ?*Decreased oxidative glutathione
c) Peroxisome
c) lipid peroxides accumulation in RBC
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d) Mitochondriad) Due to to methemoglobin formation
Explanation: In G6PD deficiency,NADPH
4.Apolipoprotein unique to Chylomicron
a) B48
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level is reduced ,so Decreasedb) B100
ReducedGlutathione cannot be formed
c) A1
...>Which leads to accumulation of free
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d) CIIradicals(Lipid peroxides also)
Option b says decreased oxidative Glutathione
5.Repeat nucleotide in Telomere results in
Qn 10.In a case of classic homocystinuria
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a) Decreased cell divisionwhat should be supplemented in the diet to
b) Increased cell division)
c)Addition of Poly A tail
prevent heart attacks
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d)Addition of SNPsa) pyridoxine.
b) methionine
6.What is the first physiological response
c) methyl cobalmine
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following hypoglycemiad) Niacin
a) Increase in cortisol
Qn 11 Endoplasmic reticulum is not site for ?
b) Decrease in insulin level
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a) Glycolysisc) Increase in epinephrine
b) Protein synthesis
d) Increase in Glucagon
Ref Stryer-Biochemistry 7/e Also Harper 30/e
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c) Protein packageThe early fasting state. The blood-glucose
d) Cell signaling
level begins to drop several hours after a
meal, leading to a decrease in insulin
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secretion and a rise in glucagon secretion;glucagon is secreted by the cells of the
pancreas in response to alow blood-sugar level
in the fasting state. Just as insulin signals the
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12.Which of the following is wrongly matchedfed state, glucagon signals the starved state.
a) Folate-Anaemia
It serves to mobilize glycogen stores when
b) Zinc-Immunodeficiency
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there is no dietary intake of glucose. The mainc) Iodine ?Dry Skin(This is the least
target organ of glucagon is the liver.
matched)
7.False about Gauchers disease
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d) Iron-Anaemiaa) Enzyme replacement therapy not available
b) Splenomegaly is massive and progressive
13.DNA melting depends on
c) Infants present with opisthotonus and
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a) Length of DNAspasm
b) Hydrogen bond between Purines
d) ?No mental retardation
c) ?Hydrogen bond between Pyrimidines
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d) G-C Pairs8. Denaturation of Proteins
a) Primary structure intact
14.Enzyme that remove hydrogen from
b) ?Secondary structure intact
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substrate to Oxygen isc) ?Tertiary structure intact
a) Oxygenase
d) ?No loss of folding
b) Oxidase
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c) Dehydrogenase9.Both Triglycerides and HDL increased
a) Smoking
15.Megaloblastic anaemia seen in
b) Athletes
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a) Ornithine Transcarbamoylase defectc) Statin/Anabolic steroid abusers
b) MSUD
d) Alcoholism
c) Citrullinemia
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Ref Harrison19/e Regular alcohol consumptiond) Orotic aciduria
has a variable effect on plasma lipid levels.
The most common effect of alcohol is to
16.Vitamin whose RDA depends on Protein
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increase plasma triglyceride levels. Alcoholintake is Pyridoxine( Reference Park)
consumption stimulates hepatic secretion of
Park clearly says RDA of Niacin depends on
VLDL, possibly by inhibiting the hepatic
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enegy intake NOT on Protein intake alone.oxidation of free fatty acids, which then
Biochemical reason-Almost all amino acid
promote hepatic triglyceride synthesis and
metabolism is dependent on Pyridoxine.
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VLDL secretion. The usual lipoprotein patternRDA of Thiamine depends on carbohydrate
seen with alcohol consumption is Type IV
intake.
(increased VLDLs), but persons with an
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May 2015underlying primary lipid disorder may develop
1. Which of the following is true about Krebs
severe hypertriglyceridemia (Type V) if they
Cycle?JipmerMay 2015
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drink alcohol. Regular alcohol use also raisesplasma levels of HDL-C.
A. Pyruvate condenses with Oxaloacetate
to form Citrate
10.Which is true about G6PD deficiency?
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B. Alpha keto Glutarate is a five Carbona) Autosomal dominant inheritance
compound
b) Does not give protection against malarial
C. Oxidative Phosphorylation occurs in
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infection.the cytoplasm only
c) Presents as chronic hemolytic anaemia
D. Krebs cycle can operate in anaerobic
d) Commonly cause neonatal jaundice
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condition11.Carbohydrate related to Blood group
2. Mousy body odour is due to ( Jipmer May
substance
2015)
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a) FucoseA. Phenyl Alanine
b) Xylose
B. Phenyl Acetate
c) Xylulose
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C. Phenyl Butazoned) Arabinose
D. Phenyl Acetyl Glutamine
3. Which is the test used to identify mRNA
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B. PRPP SynthetaseA. Southern Blot
C. Carbamoyl Phosphate Synthetase I
B. Northern Blot
D. AIR Carboxylase
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C. Western BlotD. South Western Blot
3.A vitamin derived from amino acid is
A. Biotin
B. Pantothenic acid
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4. Regarding Hb A1 C true isC. Niacin
D. Folic acid
A. Hemoglobin with a Sugar moiety
B. Cannot be used to stratify the renal
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4.N Acetyl Cysteine replenishescomplication in Diabetes Mellitus
C. Increase in Diabetic patient with
A. Glutathione
Sickle cell anaemia
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B. GlycineD. Absent in people without Diabetes
C. Glutamate
mellitus
D. ?
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5 .True about bilirubin is5.Enzyme common for synthesis of both
ketone bodies and Cholesterol
A. Conjugated with Glycerine
B. Facilitate absorption of carbohydrates
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A. HMG CoA Reductasefrom diet.
B. HMG CoA Synthase
C. Is a steroid
C. Acetyl CoA Carboxylase
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D. Bound to Albumin in circulationD. HMG CoA Lyase
6.About DNA which of the following is true
6.Glucose intolerance is seen in deficiency of
A. The nucleotide of one strand form
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A. Chromiumbonds with nucleotide of opposite
B. Selenium
strand.
C. Molybdenum
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B. Cytosine and Uracil differ by oneD. Zinc
ribose sugar
7. Mucopolysaccharide that doesn't
C. The information from DNA is copied in
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containUronic acid residue isthe form of tRNA
D. Each nucleotide pair includes two
a) Heparan Sulphate
purines.
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b) Heparinc) Chondroitin Sulphate
Nov 2014
d) Keratan Sulphate
1.Northern blot is used for
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8.True statement regarding telomerase isA. DNA
a) It is a reverse transcriptase
B. RNA
b) Active in somatic cells
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C. Proteinc) Involved in translation
D. Antigen
d) Helps in shortening of DNA
2.Rate limiting enzyme of Purine synthesis is
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9.Function of Pseudouridine arm of tRNAA. PRPP Glutamyl Amidotransferase
a) Helps in initiation of translation
15.Substrate level Phosphorylation not seen
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b) Serves as the recognition site of aminoin:
acyl tRNA synthetase
a. PhosphoFructokinase
c) Recognises the triple nucleotide codon
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b. PhosphoGlycerate kinasepresent in the mRNA.
c. Succinate Thiokinase
d. Pyruvate Kinase
d) Helps in initiation of transcription
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Substrate level Phosphorylations are:? 1,3BisphosphoGlycerate Kinase
? Pyruvate Kinase
? Succinate Thiokinase
10.Vitamin for which RDA is based on protein
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intake isa) Niacin
b)
16 Which enzyme is absent in muscle?
Riboflavin
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c)(a) Glucose-6-Phosphatase
Pyridoxine
d)
(b) Glycogen phosphorylase
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Thiamine(c) Enolase
11.In PKU which is true
(d) Thiophorase
a) Tyrosine level decreases
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Enzymes absent in the muscles areb) Decreased Phenyl alanine products
? Glucose 6 Phosphatase
c) Increase in Phenyl Alanine
? Glycerol Kinase (also absent in the
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hydroxylaseWhite Adipose Tissue)
d) Dihydrobiopterin reductase deficiency
17. Ketone bodies not utilised by:
12.Enzyme increased in the fed state is
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(a) RBC (b) liver(c) brain (d) heart
a) Acetyl CoA Carboxylase
b) Glucose 6 Phosphatase
c) CPS-1
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18. Which of the following is false aboutheparin?
d) HMGCoA Oxidase
(a) Releases lipoprotein lipase
13.All are poly unsaturated fatty acid except
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(b) Releases hormone sensitive lipase(c) It is an anticoagulant
a) Linoleic acid
(d) It is a Glycosaminoglycan
b) Palmitic acid
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19.. Which of the following is a Lyase?c) Arachidonic acid
(a) Aldolase B
d) Eicosa Pentaenoic acid
(b) Acetyl Co A Synthetase
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14.Epigenetic changes among the following(c) Fatty Acyl CoA Dehydrogenase
are all except
(d) Acetyl CoA Carboxylase
Some examples of Lyases are
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a) SnRNA interference? HMG CoA Lyase
b) Poly A tailing of the mRNA
? ArgininoSuccinateLyase
c) Histone acetylation
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? ATP Citrate Lyased) DNA methylation
? Aldolase
? Fumarase
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20. Insulin dependent glucose transport(c) hypokalemia
not present in:)
(d) ketone bodies
a. Liver
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Factors that favour Insulin secretion are:b. Adipose tissue
? Glucose
c. Heart
? Amino Acids
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d. Skeletal muscles? Free fatty Acid
21. Which is true about enzyme kinetics
? Ketone Bodies
for competitive inhibition:
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? Glucagon(a) low km high affinity
? Secretin
(b) high km high affinity
? Sulfonyl Urea drugs tolbutamide
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(c) High Km low affinityand Glybiuride
(d) Low Km low affinity
Factors that block the release of Insulin:
Features of competitive inhibition:
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? Epinephrine? Km increases, hence the affinity is
? Norepinephrine
lowered
Insulin and serum Potassium:
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? Vmax remains the same? Insulin pumps extracellular K+ to
Features of noncompetitive Inhibition:
intracellular compartment.
? Km remains the same
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? Hence insulin causes Hypokalemia? Vmax decreases
? But Hypokalemia never favour insulin
Significance of Km (Michaelis Constant):
secretion
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Km is substrate concentration at25.Lipoprotein a resembles: (Jipmer 2014)
1/2Vmax
(a) plasminogen (b) plasmin
Constant for an enzyme substrate pair
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(c) thrombin (d) prothrombinIt is called signature of the enzyme
? Higher the Km ,lower is the affinity of
26 Which is a reverse transcriptase:
the enzyme towards the substrate
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(a) Topoisomerase? Lower the Km,higher is the affinity of
(b) Telomerase
the enzyme towards the substrate
(c) RNA polymerase II
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23 Regarding electron transport chain which(d) DNA polymerase alpha
is true:
Reverse transcriptase is RNA dependent DNA
(a) Cyanide stops the ETC but allows
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Polymerase:production of AT
Option A: Topoisomerase is a nicking
(b) Oligomycin deplete the ADP
resealing enzyme,not a reverse transcriptase
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(c) Atractiloside inhibit the protonOption
B:
Telomerase
has
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reversetransfer through FoSubcomplex
transcriptase activity.
(d) Aspirin cause uncoupling of
Option C: RNA polymerase II is an DNA
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Oxidative phosphorylationdependent RNA Polymerase, not a reverse
Option A: Cyanide inhibit Complex IV of ETC,
transcriptase
and therefore totally arrest respiration
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Option D: DNAP alpha is a eukaryotic DNAOption B: Oligomycin blocks the flow of
dependent DNA Polymerase But also has
protons through the ATP Sythase
Primase activity. Primase is DNA dependent
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Option C: Atractyloside inhibit the transportRNA Polymerase, not a reverse transcriptase
of ADP and ATP.
27.Which of the following is not an aldose?
Option D: High dose Aspirin is an uncoupler
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of Oxidative Phosphorylation.A. Glucose
Hence the answer is d
B. Mannose
24. Which of the following will not favour
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C. Fructosefavour insulin secretion?
D. Galactose
(a) glucagon
28.The enzyme deficient in Refsum's disease
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(b) amino acidA. Phytanic acid Oxidase
34.Glycogenolysis in the muscle do not raise
B. Lysyl Hydroxylase
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the blood glucose due to lack of which enzyme?C. Malate Dehydrogenase
D. DNA Polymerase
A. Arginino Succinate Lyase
B. Lactate Dehydrogenase
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29.FAD dependent reaction isC. Glucose 6 Phosphatase
D. Pyruvate Kinase
A. Succinate to Fumarate
B. Fumarate to Malate
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35.Major function of HMP is to provideC. Glucose to Glycogen
D. Ketoglutarate to Succinate
A. ATP
B. GTP
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C. NADHD. NADPH
36.In which of the following copper acts as
coenzyme
30.One of the following is obtained in the by
--- Content provided by FirstRanker.com ---
A. Carboxy peptidasebeta oxidation of odd chain fatty acids
B. Pyruvate Carboxylase
C.
A.
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Cytochrome OxidaseAcetyl CoA + Acetyl CoA
D.
B.
Carbonic Anhydrase
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Acetyl CoA + Propionyl CoAC. Propionyl CoA + Propionyl CoA
37.The source of Ammonia in urine is
D. Acetyl CoA alone
A. Glutaminase
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31.The number of double bonds in theB. Urease
Arachidonic acid
C. Arginase
D.
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A.Glutamate Dehydrogenase
1
B. 2
38.Which of the following is not from
--- Content provided by FirstRanker.com ---
C. 3Aspartate
D. 4
A. Anserine
32.Restriction Endonuclease is used in
--- Content provided by FirstRanker.com ---
B. GlucoseC.
A.
Oxaloacetate
RFLP
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D.B.
Asparagine
PCR
C. FISH
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39.The function of Cori's CycleD. SDS-PAGE
A. Fatty acid transport
33.The Maple Syrup Urine disease is due to
B. 2,3 Bisphospho Glycerate transport
--- Content provided by FirstRanker.com ---
defect inC. Reutilisation of Lactate
D.
A.
Transport of Amino acid across the
--- Content provided by FirstRanker.com ---
Transaminationmembrane
B. Hydroxylation
C. Deamination
40.Carbon Dioxide is not released in the
--- Content provided by FirstRanker.com ---
D. Decarboxylationfollowing reactions
A. Hydroxy Butyrate Dehydrogenase
B. KetoGlutarate Dehydrogenase
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B. ArginineC. Isocitrate Dehydrogenase
C. Glutamate
D. Malic Enzyme
D. Aspartate
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41.The conversion of Uroporphyrinogen III to47.The synthesis of 1 peptide bond involves
Coproporphyrinogen III is an example of
A. 1 ATP
A. Deamination
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B. 2 ATPsB. Hydrogenation
C. 3 ATPs
C. Decarboxylation
D. 4 ATPs
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D. Dehydrogenation48.Selenium is a cofactor in the following
42.Which is an activator of LCAT?
enzyme
A. Apo E
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A. Glutathione PeroxidaseB. Apo AI
B. Cytochrome Oxidase
C. Apo B48
C. Cytochrome Reductase
--- Content provided by FirstRanker.com ---
D. Apo B100D. Xanthine Oxidase
49.Thiamine act as a cofactor in
A. Pyruvate to Oxaloacetate
B. Malonate to Oxaloacetate
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43.Which of the following is a glycolipid?C. Succinate to Fumarate
D.
A.
Pyruvate to Acetyl CoA
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CerebrosideB. Plamalogen
50.Enzyme not involved in DNA replication is
C. Sphingomyelin
D. Lecithin
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A. TelomeraseB. Reverse Transcriptase
44.All the enzyme activities are increased in
C. Restriction Endonuclease
fasting state EXCEPT
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D. DNA LigaseA. Acety CoA Carboxylase
51.Copper is required in the collagen
B. Carnitine Acyl Transferase
synthesis for
--- Content provided by FirstRanker.com ---
C. PEPCKD. Pyruvate Carboxylase
A. Lysyl Hydroxylase
B. Lysyl Oxidase
45.Which of the following is both polar and
--- Content provided by FirstRanker.com ---
C. Prolyl Hydroxylaseionic?
D. Prolyl Oxidase
A. Arginine
52.Amino acid not involved in Protein
--- Content provided by FirstRanker.com ---
B. Asparaginesynthesis
C. Glutamine
D. Leucine
A. Ornithine
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B. Alanine46.The amino acid which on decarboxylation
C. Asparagine
gives rise to a potent vasodilator
D. Cysteine
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A. Histidine53.Niemann Pick Disease is due to deficiency
D. Amino acids
of
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60.All the following require Biotin as aA. Sphingomyelinase
coenzyme except
B. Gangliosidoses
C. Glucucerebrosidase
--- Content provided by FirstRanker.com ---
A. Propionyl CoA CarboxylaseD. Ceramidase
B. Acetyl CoA Carboxylase
C. Pyruvate Carboxylase
54.Tay Sachs disease is due to accumulation of
--- Content provided by FirstRanker.com ---
D. Pyruvate DehydrogenaseA. GM2 Ganglioside
61.The major fuel in the brain after several
B. GM1 Ganglioside
weeks of starvation is:
--- Content provided by FirstRanker.com ---
C. GlucocerebrosideD. Galactocerebroside
A. Glucose
B. Fatty Acid
55.Hereditary Fructose Intolerance is due to
--- Content provided by FirstRanker.com ---
C. Hydroxy Butyratedeficiency of
D. Glycerol
A. Aldolase B
62.The storage Tri acyl Glycerol are
--- Content provided by FirstRanker.com ---
B. Aldolase Ahydrolysed by
C. Fructokinase
D. Sucrase
A. Pancreatic Lipase
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B. Lipoprotein Lipase56.Second messenger is produced from
C. Lysosomal Lipase
D. Hormone sensitive Lipase
A. Phosphatidyl inositol
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B. Phosphatidyl SerineC. Phosphatidyl Choline
D. None
57.Pseudotumour cerebri is caused by
hypervitaminosis of
--- Content provided by FirstRanker.com ---
A. Vitamin DB. Vitamin A
C. Vitamin E
D. Vitamin K
58.Suicidal enzyme is
--- Content provided by FirstRanker.com ---
A. CyclooxygenaseB. Lipoxygenase
C. Dehydrogenase
D. Pyruvate Kinase
59.Bile acids are derived from
--- Content provided by FirstRanker.com ---
A. BilirubinB. Fatty acid
C. Cholesterol