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  • The Ultimate Guide to the NEET PG 2025 Syllabus & Exam Pattern

    A Comprehensive Guide to the NEET PG 2025 Syllabus: Subject-wise Topics and Strategic Analysis

     

     

    Part I: Strategic Framework of the NEET PG 2025 Examination

     

     

    Section 1: Examination Blueprint and Architecture

     

    The National Eligibility cum Entrance Test for Postgraduate (NEET PG) stands as the definitive, single-window examination for admission to postgraduate medical courses across India. It serves as the sole eligibility and entrance test for MD (Doctor of Medicine), MS (Master of Surgery), and PG Diploma programs, as well as Post-MBBS DNB, Direct 6-year DrNB, and NBEMS Diploma courses for the 2025 academic session. This centralized examination streamlines the selection process for admissions into 50% All India Quota seats, State Quota seats, and seats in Deemed and Central Universities.  

    The National Board of Examinations in Medical Sciences (NBEMS) is the designated authority for conducting the NEET PG examination. While the NBEMS manages the operational aspects of the test, the syllabus itself is prescribed by the National Medical Commission (NMC), the successor to the Medical Council of India (MCI). The curriculum is fundamentally based on the Graduate Medical Education (GME) Regulations of 2018, ensuring that the examination comprehensively covers the subjects and knowledge areas taught during the MBBS course. The examination is designed not merely to test theoretical knowledge but to evaluate a candidate’s practical application skills and problem-solving abilities in diverse medical fields.  

    A thorough understanding of the examination’s structural and operational parameters is the foundational first step for any aspirant aiming to devise an effective preparation strategy. The modalities of the NEET PG are precise and standardized to ensure a uniform and fair assessment process for all candidates.

    Table 1: NEET PG 2025 Examination Pattern at a Glance

    Parameter Detail
    Conducting Body National Board of Examinations in Medical Sciences (NBEMS)  

    Syllabus Authority National Medical Commission (NMC)  

    Exam Purpose Eligibility-cum-entrance test for MD/MS/PG Diploma courses  

    Exam Mode Computer-Based Test (CBT)  

    Exam Language English only  

    Total Duration 3 hours and 30 minutes (210 minutes)  

    Total Questions 200  

    Question Type Multiple-Choice Questions (MCQs) with a single correct response  

    Maximum Marks 800  

    Marking Scheme +4 for a correct answer; -1 for an incorrect answer; 0 for an unattempted question  

    The architecture of the examination, as detailed in the table, has significant strategic implications. The allocation of 210 minutes for 200 questions provides just over one minute per question, demanding exceptional time management and rapid, accurate recall. Furthermore, the negative marking scheme penalizes incorrect guesses, compelling candidates to adopt a calculated approach to answering questions where they are uncertain. This high-stakes format, with each question holding the potential for a five-mark swing (+4 for correct, -1 for incorrect), necessitates a preparation strategy that prioritizes not only knowledge acquisition but also test-taking accuracy and risk assessment.

     

    Section 2: Syllabus Architecture and Subject Weightage

     

    The NEET PG syllabus is methodically structured to mirror the academic progression of the MBBS curriculum. It is divided into three distinct phases: Pre-clinical, Para-clinical, and Clinical. This tripartite structure ensures a logical flow, beginning with the foundational sciences that explain the structure and function of the human body, moving through the subjects that bridge basic science with disease, and culminating in the clinical disciplines focused on diagnosis and patient management. In total, the syllabus encompasses 19 subjects, covering the entire breadth of undergraduate medical education.  

    For an aspirant, the most critical piece of strategic information is the subject-wise distribution of questions. This data provides an objective framework for allocating study time and resources, enabling a focus on high-yield subjects that have the greatest impact on the final score. The NBEMS provides a clear breakdown of the question distribution, which is indispensable for strategic planning.

    Table 2: Subject-wise Question Distribution for NEET PG 2025 (Based on 200-Question Pattern)

    Phase Subject Number of Questions Percentage Weightage
    Pre-clinical (Part A) 50 25.0%
    Anatomy 17 8.5%
    Physiology 17 8.5%
    Biochemistry 16 8.0%
    Para-clinical (Part B) 100 50.0%
    Pathology 25 12.5%
    Pharmacology 20 10.0%
    Microbiology 20 10.0%
    Social & Preventive Medicine (SPM) 25 12.5%
    Forensic Medicine (FMT) 10 5.0%
    Clinical (Part C) 150 (as per some sources) or 100 (as per others) Varies (See note below)
    General Medicine (incl. Dermatology, Venereology, Psychiatry) 45 22.5%
    General Surgery (incl. Orthopaedics, Anaesthesia, Radiodiagnosis) 45 22.5%
    Obstetrics & Gynaecology 30 15.0%
    Paediatrics 10 5.0%
    Ophthalmology 10 5.0%
    Otorhinolaryngology (ENT) 10 5.0%
    Total 200 100.0%

    Note: There are slight variations in how sources group subjects. The table above reflects the most common distribution where the total questions sum to 200. The core takeaway remains the relative weightage of each subject.  

    A careful analysis of this distribution reveals several crucial patterns that must inform any serious preparation strategy. The most salient feature is the profound dominance of clinical subjects. The two major clinical blocks, General Medicine and General Surgery (along with their allied specialties), together account for 90 questions, representing a staggering 45% of the entire examination. When combined with Obstetrics & Gynaecology (30 questions), these three subjects alone constitute 60% of the paper. This heavy clinical orientation signifies that the exam’s primary purpose is to assess the candidate’s aptitude for clinical reasoning, diagnosis, and management, rather than just rote memorization of preclinical facts. Consequently, preparation must pivot towards a case-based, problem-solving approach that integrates foundational knowledge into clinical scenarios.  

    Delving deeper into the data, a core group of five subjects emerges as the backbone of the NEET PG examination. These “Big Five” are General Medicine (45), General Surgery (45), Obstetrics & Gynaecology (30), Pathology (25), and Social & Preventive Medicine (25). Collectively, these subjects contribute 170 out of 200 questions, or 85% of the total marks. This statistical reality provides an unambiguous directive for aspirants: achieving mastery in these five subjects is not merely advantageous; it is a prerequisite for securing a competitive rank. While every subject contributes to the final score, the bulk of study time and effort must be strategically allocated to these high-yield areas.  

    Conversely, the table also highlights subjects with lower question volumes, such as Forensic Medicine, Paediatrics, Ophthalmology, and ENT, each contributing 10 questions. This does not diminish their importance, as 40 marks from these subjects can be a significant rank determinant. However, it does suggest that the study approach for these subjects should be highly efficient and targeted, focusing on the most frequently tested, high-yield topics rather than exhaustive, encyclopedic coverage.  

    Finally, the pre-clinical subjects—Anatomy, Physiology, and Biochemistry—collectively account for 50 questions, or 25% of the exam. While their individual weightage is moderate, their combined contribution is substantial. More importantly, they form the conceptual bedrock upon which all para-clinical and clinical knowledge is built. A weak foundation in these subjects will inevitably compromise a candidate’s ability to understand and answer complex, integrated clinical questions. Therefore, neglecting these subjects based on their individual question count would be a grave strategic error.  

     

    Part II: The Pre-Clinical Foundation: Detailed Syllabus

     

     

    Section 3: Anatomy

     

    Strategic Importance: With an allocation of approximately 17 questions, Anatomy holds a significant position in the pre-clinical section. Its importance extends far beyond these direct questions, as a robust understanding of human anatomy is indispensable for comprehending subjects like Surgery, Orthopaedics, Radiodiagnosis, and Obstetrics & Gynaecology. The questions often test the application of anatomical knowledge in clinical contexts.  

    Detailed Topics: The syllabus for Anatomy is vast, covering macroscopic and microscopic structures of the human body. It is best approached regionally and systemically, with an emphasis on clinical application.

    • General Anatomy: This includes foundational knowledge of anatomical terminology, planes, and movements. It covers the general features and organization of skin, superficial and deep fascia, muscles (origin, insertion, nerve supply, action), the skeletal system (osteology), joints (arthrology), the cardiovascular system, and the lymphatic system.  
    • Gross Anatomy (Regional Approach):
      • Upper Limb: Comprehensive study of the Pectoral region, Axilla, Scapular region, Arm, Cubital fossa, Forearm, and Hand. This includes the osteology of individual bones, joints, vascular and nervous structures, and their clinical correlations.  
      • Lower Limb: Detailed examination of the Gluteal region, front and medial aspects of the thigh, back of the thigh, popliteal fossa, Hip joint, Knee joint, compartments of the leg, and the anatomy of the foot and its arches.  
      • Thorax: Study of the thoracic cage, intercostal spaces, Diaphragm, Mediastinum and its contents, Pericardium and Heart, Lungs and Pleura, and the Tracheobronchial tree.  
      • Abdomen and Pelvis: Detailed anatomy of the anterior and posterior abdominal walls, inguinal canal, abdominal cavity, peritoneum and its folds, pelvic walls and floor, and the viscera of the abdomen and pelvis including the gastrointestinal tract, liver, spleen, pancreas, kidneys, and reproductive organs. It also includes the male external genitalia and the perineum.  
      • Head and Neck: This is an extensive section covering skull osteology, the scalp, face, and parotid gland. It includes the triangles of the neck, deep cervical fascia, temporal and infratemporal fossae, cranial cavity and its contents (dural venous sinuses), the orbit, and the detailed anatomy of the mouth, pharynx, palate, larynx, tongue, nasal cavity, and the organs of hearing and equilibrium.  
      • Back: A thorough understanding of the vertebral column, its curvatures, individual vertebrae, intervertebral discs, and cranio-vertebral joints is required.  
    • Neuroanatomy: This critical area involves the detailed structure of the central and peripheral nervous systems. Topics include the cerebrum, cerebellum, brainstem (medulla, pons, midbrain), spinal cord and its tracts, meninges, ventricular system and cerebrospinal fluid (CSF) circulation, cranial nerves, and major sensory and motor pathways.  
    • Histology (Microanatomy): The microscopic structure of the four basic tissues—Epithelium, Connective tissue (including bone and cartilage), Muscle tissue, and Nervous tissue—is fundamental. This extends to the microanatomy of all major organ systems.  
    • Embryology: This section covers gametogenesis, fertilization, implantation, and the developmental events from the first to the eighth week. It includes the development of the pharyngeal arches, fetal membranes (placenta, amnion), and the organogenesis of major systems, with a special focus on the cardiovascular, nervous, and gastrointestinal systems. Congenital anomalies and their developmental basis are a high-yield area.  
    • Applied Anatomy: This includes surface anatomy, cross-sectional anatomy of the thorax, abdomen, and pelvis, and the anatomical basis of clinical procedures and radiological imaging (radiography).  

    The modern approach to testing anatomy in NEET PG has evolved significantly from simple recall of structures. An analysis of the syllabus, which explicitly mentions “clinical correlations,” “surface markings,” and “radiography,” points towards a paradigm shift. The examination now prioritizes the application of anatomical knowledge to solve clinical problems. For instance, a question is less likely to ask for the simple identification of a nerve and more likely to present a scenario of a fracture at a specific bony landmark and ask which nerve is most susceptible to injury and what the resulting clinical deficit (e.g., foot drop, wrist drop) would be. This requires a multi-layered understanding that links gross anatomy (bone and nerve location), clinical medicine (fracture patterns), and physiology (nerve function). Therefore, an effective study strategy involves integrating anatomy with clinical subjects. When studying the brachial plexus, one should concurrently review the clinical presentations of Erb’s and Klumpke’s palsies. Similarly, the study of abdominal quadrants should be linked to the differential diagnosis of pain in those regions, a core concept in General Surgery and Medicine.  

     

    Section 4: Physiology

     

    Strategic Importance: Accounting for approximately 17 questions, Physiology is a cornerstone of medical science and a critical component of the NEET PG examination. It provides the logical framework for understanding health and the aberrations that lead to disease. A strong foundation in physiology is essential for mastering General Medicine, Pharmacology, and Pathology, as it explains the “why” behind clinical signs, symptoms, and therapeutic interventions.  

    Detailed Topics: The syllabus covers the dynamic functions of the human body, from the cellular level to the integrated actions of organ systems.

    • General Physiology: This foundational area includes the concepts of homeostasis, the composition and dynamics of body fluid compartments, cell membrane structure, and transport mechanisms across the cell membrane, including the generation of resting membrane potential.  
    • Nerve and Muscle Physiology: This section covers the properties of nerve fibers, the generation and propagation of action potentials, the structure and function of the neuromuscular junction, and the molecular mechanisms of skeletal, cardiac, and smooth muscle contraction.  
    • Blood Physiology (Hematology): Topics include the composition of blood, functions of plasma proteins, formation and function of red blood cells (RBCs), white blood cells (WBCs), and platelets. It also covers hemoglobin, erythropoiesis, blood groups, and the intricate cascade of blood coagulation.  
    • Cardiovascular System (CVS): A high-yield area covering the properties of cardiac muscle, the cardiac cycle, regulation of cardiac output and heart rate, hemodynamics, arterial blood pressure regulation, interpretation of the electrocardiogram (ECG), and the physiology of regional circulations (coronary, cerebral, cutaneous).  
    • Respiratory System: This includes the mechanics of breathing, lung volumes and capacities (spirometry), pulmonary and alveolar ventilation, gas exchange and transport ( and ), ventilation-perfusion (V/Q) relationships, and the neural and chemical regulation of respiration.  
    • Renal System: This covers the structure and function of the nephron, glomerular filtration rate (GFR) and its regulation, tubular reabsorption and secretion, the counter-current mechanism for urine concentration, micturition, and the kidney’s crucial role in regulating fluid, electrolyte, and acid-base balance.  
    • Gastrointestinal (GI) System: Topics include the composition and functions of saliva, gastric, pancreatic, and intestinal secretions. It also covers GI motility, the processes of digestion and absorption of nutrients, and the metabolic functions of the liver.  
    • Endocrinology: This section details the synthesis, secretion, transport, mechanism of action, and physiological effects of hormones from the hypothalamus, pituitary, thyroid, parathyroid, adrenal cortex and medulla, and endocrine pancreas. The hormonal regulation of metabolism is a key focus.  
    • Reproductive Physiology: This includes the study of male and female reproductive hormones, spermatogenesis, the menstrual cycle, physiological changes during pregnancy, parturition (childbirth), and lactation.  
    • Neurophysiology: A vast topic covering the organization of the nervous system, sensory receptors and pathways, the motor system (reflexes, cerebellum, basal ganglia, motor cortex), the physiology of pain, the functions of the autonomic nervous system, and higher cerebral functions such as sleep, memory, and learning. The physiology of the special senses (vision, hearing, taste, smell) is also included.  

    The emphasis in Physiology questions is increasingly on understanding regulatory mechanisms and interpreting graphical data. The syllabus is replete with topics like the cardiac cycle, respiratory volumes, and ECG, which are dynamic processes frequently visualized through graphs, loops, and tracings. The examination aims to test a candidate’s ability to analyze this data to deduce a physiological or pathological state. For example, a question might present a pressure-volume (PV) loop of the left ventricle with altered morphology and ask the candidate to identify the underlying condition, such as aortic stenosis, mitral regurgitation, or diastolic dysfunction. Similarly, a spirometry graph might be used to differentiate between obstructive and restrictive lung diseases. This approach assesses a core clinical skill: the ability to translate graphical information into a meaningful physiological diagnosis. Therefore, preparation must extend beyond memorizing facts to developing a deep conceptual understanding of these dynamic processes and proficiency in interpreting their graphical representations.  

     

    Section 5: Biochemistry

     

    Strategic Importance: With approximately 16 questions, Biochemistry is a vital pre-clinical subject that forms the molecular basis of health and disease. Its principles are fundamental to understanding genetics, metabolic disorders, nutrition, and the molecular diagnostics that are increasingly central to modern medicine. It has strong integrations with Paediatrics, General Medicine, and Pathology.  

    Detailed Topics: The syllabus for Biochemistry covers the chemical processes of life, from the structure of individual molecules to the complex integration of metabolic pathways.

    • Biomolecules (Chemistry of): This includes the structure, classification, properties, and biological functions of the major classes of biomolecules: carbohydrates (monosaccharides, disaccharides, polysaccharides), lipids (fatty acids, triglycerides, phospholipids, cholesterol), proteins (amino acids, peptide bonds, levels of protein structure), and nucleic acids (nucleotides, DNA, RNA).  
    • Enzymology: A key area focusing on the classification and nomenclature of enzymes, their mechanism of action, factors affecting enzyme activity, enzyme kinetics (including Michaelis-Menten equation and Lineweaver-Burk plot), types of enzyme inhibition (competitive, non-competitive), regulation of enzyme activity, and the clinical significance of isoenzymes.  
    • Metabolic Pathways and their Regulation: This is the core of biochemistry and includes:
      • Carbohydrate Metabolism: Glycolysis, citric acid (TCA) cycle, gluconeogenesis, glycogen metabolism, and the hexose monophosphate (HMP) shunt. Metabolism of fructose and galactose is also important.  
      • Lipid Metabolism: Beta-oxidation of fatty acids, ketogenesis, biosynthesis of fatty acids and cholesterol, and metabolism of lipoproteins (chylomicrons, VLDL, LDL, HDL).  
      • Amino Acid and Protein Metabolism: General reactions of amino acids (transamination, deamination), the urea cycle, and the metabolism of individual amino acids, especially aromatic and sulfur-containing ones. Synthesis of heme and its breakdown to bilirubin (jaundice) is a high-yield topic.  
      • Integration of Metabolism: Understanding how these pathways are interconnected and regulated during fed, fasting, and starvation states.  
    • Molecular Biology: This section covers the structure of DNA and RNA, DNA replication, transcription (synthesis of RNA), translation (protein synthesis), and the regulation of gene expression. It also includes DNA repair mechanisms and the principles of molecular biology techniques like Polymerase Chain Reaction (PCR), Southern/Northern/Western blotting, and ELISA.  
    • Nutrition: The biochemical roles of vitamins (fat-soluble and water-soluble) and minerals, their dietary sources, daily requirements, and the clinical manifestations of their deficiencies or excesses.  
    • Clinical and Applied Biochemistry: This section links biochemical knowledge to clinical practice. It includes the mechanism of hormone action, organ function tests (liver function tests, kidney function tests), acid-base balance and disorders, electrolytes, the biochemical basis of cancer (oncogenesis and tumor markers), and the molecular basis of genetic diseases.  
    • Genetics: This includes topics like the haem synthesis pathway and its defects (porphyrias), haemoglobinopathies (sickle cell anemia), and various inborn errors of metabolism such as urea cycle disorders, phenylketonuria, and lysosomal storage diseases (e.g., Tay-Sachs, Gaucher disease).  

    A significant portion of the Biochemistry syllabus and the resulting examination questions are built around the “metabolic error” paradigm. The syllabus repeatedly details specific metabolic pathways alongside their associated inborn errors of metabolism. This indicates that the exam will rigorously test the candidate’s ability to connect a specific biochemical pathway, the deficient enzyme within it, the resulting accumulation of a substrate or deficiency of a product, and the consequent clinical picture. A typical question will present a clinical vignette, often involving a neonate or child with symptoms like failure to thrive, developmental delay, or specific food intolerance, and ask the candidate to identify the most probable enzyme deficiency or the name of the disorder. To prepare effectively, aspirants must visualize these pathways as flowcharts rather than as a series of disconnected reactions. They must be able to trace each pathway, identify its rate-limiting and regulated steps, and logically predict the clinical consequences of a block at any given point. This approach directly bridges the gap between fundamental biochemistry and its clinical application in Paediatrics and General Medicine.  

     

    Part III: The Para-Clinical Bridge: Detailed Syllabus

     

     

    Section 6: Pathology

     

    Strategic Importance: With an allocation of 25 questions, Pathology is the most heavily weighted para-clinical subject and one of the top three subjects overall in the NEET PG exam. It serves as the scientific foundation of medicine, explaining the structural and functional changes that underlie disease. A mastery of pathology is non-negotiable, as its principles are interwoven with every clinical specialty, providing the language and logic for diagnosis.  

    Detailed Topics: The syllabus for Pathology is extensive, covering the causes (etiology) and mechanisms (pathogenesis) of disease, from the cellular level to the effects on entire organ systems.

    • General Pathology: This is the conceptual core of the subject.
      • Cellular Pathology: Cell injury (reversible and irreversible), cellular adaptations (hypertrophy, hyperplasia, atrophy, metaplasia), and cell death (necrosis, apoptosis, and newer concepts like ferroptosis).  
      • Inflammation and Repair: Acute and chronic inflammation, chemical mediators, cellular events, wound healing, and fibrosis.  
      • Hemodynamic Disorders: Edema, hyperemia and congestion, hemorrhage, thrombosis, embolism, infarction, and shock.  
      • Pathological Accumulations: Amyloidosis and pathological calcification.  
    • Immunopathology: This section covers diseases of the immune system, including the four types of hypersensitivity reactions, the pathogenesis of major autoimmune diseases (e.g., Systemic Lupus Erythematosus, Rheumatoid Arthritis, Sjogren’s syndrome), organ transplant rejection, and primary and secondary (including AIDS) immunodeficiency disorders.  
    • Neoplasia: A high-yield topic covering the nomenclature of tumors, the characteristics of benign and malignant neoplasms, epidemiology of cancer, molecular basis of carcinogenesis (oncogenes, tumor suppressor genes), tumor progression, grading and staging of cancers, and the clinical aspects of neoplasia including tumor markers.  
    • Genetic and Pediatric Diseases: This includes single-gene disorders (Mendelian), complex multigenic disorders, cytogenetic disorders (e.g., Down syndrome, Turner syndrome, Klinefelter syndrome), and common pediatric tumors.  
    • Systemic Pathology: This vast section applies the principles of general pathology to individual organ systems, covering the etiology, pathogenesis, macroscopic (gross) features, and microscopic (histological) features of diseases affecting:
      • Cardiovascular System (atherosclerosis, IHD, valvular diseases)
      • Respiratory System (COPD, pneumonia, lung cancers)
      • Kidney and Urinary Tract (glomerulonephritis, pyelonephritis, renal tumors)
      • Gastrointestinal Tract (peptic ulcer, IBD, GI tumors)
      • Hepatobiliary System and Pancreas (hepatitis, cirrhosis, gallstones, pancreatitis)
      • Central Nervous System (infections, tumors, degenerative diseases)
      • Endocrine System (thyroiditis, endocrine tumors)
      • Reproductive Systems (male and female)
      • Musculoskeletal System (osteomyelitis, bone tumors).  
    • Hematopathology: This is a major subsection covering disorders of red blood cells (anemias: microcytic, macrocytic, hemolytic), white blood cells (leukopenias, leukocytosis, acute and chronic leukemias, myeloproliferative neoplasms, lymphomas: Hodgkin and Non-Hodgkin), and plasma cell disorders (multiple myeloma). It also includes bleeding disorders and principles of blood banking and transfusion medicine.  
    • Clinical Pathology and Laboratory Techniques: This includes principles of basic diagnostic cytology (e.g., Pap smear), histopathology techniques, and the application of immunohistochemistry in diagnosis.  

    The study of pathology for NEET PG hinges on the central importance of morphology and its correlation with the molecular basis of disease. The examination heavily tests the candidate’s ability to recognize characteristic morphological changes, both at the gross (macroscopic) and microscopic levels, and to link these findings to their underlying molecular pathogenesis. The syllabus covers both broad concepts like “Neoplasia” and specific molecular details like the “molecular basis of cancer”. This strongly suggests that a significant number of questions will be image-based, presenting a photograph of a gross specimen or a histology slide. The candidate will be expected to identify key pathognomonic features—such as Reed-Sternberg cells in Hodgkin lymphoma, psammoma bodies in papillary thyroid carcinoma, caseous necrosis in tuberculosis, or Negri bodies in rabies—and correlate them with the correct diagnosis. Furthermore, high-level questions may require linking these morphological findings to a specific genetic mutation, such as the BCR-ABL translocation in Chronic Myeloid Leukemia or the BRAF mutation in melanoma. Consequently, preparation for pathology must be intensely visual. Aspirants should dedicate substantial time to studying pathology atlases, high-quality online image banks, and image-based question modules. The key to success is to build a strong mental framework connecting a disease’s name, its causative molecular defect, its characteristic microscopic appearance, and its typical gross presentation.  

     

    Section 7: Pharmacology

     

    Strategic Importance: With 20 questions, Pharmacology is a high-yield para-clinical subject with immense practical relevance. It is the science of drugs and their effects on living systems. A thorough knowledge of pharmacology is essential for safe and effective prescribing, making it a critical area of assessment for any aspiring postgraduate. Its principles are directly applicable in every clinical specialty, most notably in General Medicine.  

    Detailed Topics: The syllabus is structured around general principles and the systemic classification of drugs.

    • General Pharmacology: This section lays the foundation for the entire subject.
      • Pharmacokinetics: The study of what the body does to the drug, encompassing Absorption, Distribution, Metabolism, and Excretion (ADME). Concepts like bioavailability, volume of distribution, clearance, and half-life are crucial.  
      • Pharmacodynamics: The study of what the drug does to the body, including mechanisms of drug action, dose-response relationships, and the structure and function of receptors.  
      • Core Concepts: Drug interactions, adverse drug reactions (ADRs), pharmacovigilance, pharmacogenetics, and principles of clinical trials.  
    • Autonomic Nervous System (ANS) Pharmacology: A key topic covering cholinergic (parasympathomimetic) and anti-cholinergic drugs, as well as adrenergic (sympathomimetic) and anti-adrenergic drugs.  
    • Autacoids and their Antagonists: This includes drugs acting on histamine and serotonin receptors, eicosanoids (prostaglandins, leukotrienes), and Non-Steroidal Anti-Inflammatory Drugs (NSAIDs).  
    • Systemic Pharmacology: This large section covers drugs acting on various organ systems:
      • Cardiovascular System: Drugs used in the management of hypertension, angina pectoris, myocardial infarction, heart failure, cardiac arrhythmias, and dyslipidemia.  
      • Central Nervous System (CNS): Sedative-hypnotics, general and local anesthetics, anti-epileptic drugs, drugs for Parkinsonism, anti-psychotics, anti-depressants, mood stabilizers, and opioids.  
      • Endocrine System: Drugs for diabetes mellitus (insulin, oral hypoglycemics), thyroid and anti-thyroid drugs, corticosteroids, and drugs affecting bone mineral metabolism (for osteoporosis).  
      • Gastrointestinal and Respiratory Systems: Drugs for peptic ulcer disease and GERD, anti-emetics, laxatives, anti-diarrheal agents, and drugs for bronchial asthma and cough.  
      • Hematology: Drugs affecting coagulation, including anticoagulants, antiplatelet agents, and thrombolytics, as well as drugs for anemia (hematinics).  
    • Chemotherapy: This is a vast and high-yield area covering:
      • Antimicrobial Agents: A detailed study of various classes of antibacterials, their mechanisms of action, spectrum of activity, and resistance patterns. It also includes anti-tubercular, anti-leprotic, anti-fungal, anti-viral (including anti-retroviral), and anti-protozoal drugs.  
      • Anti-cancer Drugs (Antineoplastics): Classification, mechanisms of action, and toxicities of cytotoxic drugs, targeted therapies, and hormonal agents used in cancer treatment.  
    • Miscellaneous Topics: Immunomodulators (immunosuppressants and immunostimulants), chelating agents for heavy metal poisoning, and principles of toxicology and management of poisoning.  

    When analyzing the pattern of questions in Pharmacology, two concepts consistently emerge as being of paramount importance: the “Mechanism of Action” (MOA) and the “Adverse Drug Effects” (ADEs). While knowing the clinical use of a drug is important, the examination probes deeper into the fundamental understanding of how a drug works and what its significant side effects and contraindications are. For example, a question is unlikely to be as simple as “Which drug is used for hypertension?”. Instead, it is more likely to be framed as, “A patient being treated for hypertension develops a persistent dry cough. The drug responsible for this side effect acts by which of the following mechanisms?”. This requires the candidate to first identify the drug class (ACE inhibitors) from the characteristic side effect and then recall its mechanism of action (inhibition of angiotensin-converting enzyme). Similarly, questions frequently test knowledge of pathognomonic or life-threatening adverse effects, such as amiodarone-induced pulmonary fibrosis, clozapine-induced agranulocytosis, or statin-induced rhabdomyolysis. An efficient and highly effective preparation method is to create structured summary tables for each drug class. These tables should have dedicated columns for: Drug/Class, Mechanism of Action, Primary Clinical Uses, and Key Adverse Effects/Contraindications. This comparative approach facilitates rapid learning, aids retention, and prepares the candidate for the most common types of pharmacology questions.

     

    Section 8: Microbiology

     

    Strategic Importance: Microbiology, with its 20-question allocation, is a crucial para-clinical subject. It is the study of microorganisms, including bacteria, viruses, fungi, and parasites. A strong command of microbiology is fundamental to the diagnosis, treatment, and prevention of infectious diseases, which form a substantial portion of the caseload in General Medicine, Paediatrics, and Community Medicine.  

    Detailed Topics: The syllabus encompasses the biology of various pathogens and the host’s response to them.

    • General Microbiology: This includes the history of microbiology, principles of sterilization and disinfection, classification and properties of bacteria, bacterial growth curve, bacterial genetics (transformation, transduction, conjugation), and the dynamics of host-parasite relationships.  
    • Immunology: This section covers the components of the innate and adaptive immune systems, antigens, the structure and function of antibodies (immunoglobulins), the complement system, principles of antigen-antibody reactions (used in diagnostics), and the science of vaccines.  
    • Systematic Bacteriology: This is a major part of the syllabus, requiring detailed knowledge of important bacteria. For each major pathogen (e.g., Staphylococcus aureus, Streptococcus pyogenes, Mycobacterium tuberculosis, Escherichia coli, Salmonella, Vibrio cholerae), the candidate must know its morphology, culture characteristics, virulence factors, pathogenesis, the clinical diseases it causes, and laboratory diagnosis. This includes gram-positive and gram-negative cocci and bacilli, spirochetes (  

      Treponema, Leptospira), and atypical organisms (Chlamydia, Mycoplasma, Rickettsia).  

    • Virology: This covers the general properties of viruses, their replication cycles, and pathogenesis. Detailed study of clinically important DNA viruses (e.g., Herpesviruses, Hepatitis B virus) and RNA viruses (e.g., Influenza virus, HIV, Hepatitis C virus, Arboviruses like Dengue, Rabies virus) is required.  
    • Mycology: This section focuses on fungi of medical importance, including yeasts (Candida, Cryptococcus), molds (Aspergillus), dimorphic fungi (Histoplasma), and dermatophytes that cause superficial infections.  
    • Parasitology: This covers the life cycles, pathogenesis, clinical features, and laboratory diagnosis of major human parasites. This includes protozoa (e.g., Plasmodium (malaria), Entamoeba histolytica, Giardia lamblia, Leishmania, Toxoplasma) and helminths (worms), including nematodes (roundworms), cestodes (tapeworms), and trematodes (flukes).  
    • Applied and Clinical Microbiology: This section integrates the knowledge of pathogens with clinical medicine. It is often organized by organ system, covering the common causative agents of CNS infections, cardiovascular infections, respiratory tract infections, gastrointestinal infections, and genitourinary infections. It also includes topics like zoonotic diseases, nosocomial (hospital-acquired) infections, and biomedical waste management.  

    The core of microbiology testing in NEET PG revolves around the “Organism-Disease-Diagnosis” triad. The examination consistently assesses the candidate’s ability to forge a strong link between a specific microorganism, the clinical disease or syndrome it produces, and the definitive laboratory method used for its diagnosis. The syllabus is structured both by organism type (Bacteriology, Virology) and by clinical presentation (CNS infections, GI infections), reinforcing the need for this integrated knowledge. A typical question might describe a clinical scenario—for instance, a patient with meningitis following a head injury—and ask for the most likely causative organism (  

    Streptococcus pneumoniae). Another question might present a patient with fever, headache, and confusion, and state that the CSF analysis by PCR is positive, asking to identify the gold standard test for Herpes Simplex Encephalitis. To excel in this subject, study should be organized around this triad. For every important microbe, the aspirant must create a mental or written profile that includes: the organism’s key characteristics, the disease(s) it causes, its mode of transmission, its primary virulence factors, and, most importantly, the specimen of choice and the gold-standard diagnostic test(s).

     

    Section 9: Forensic Medicine and Toxicology (FMT)

     

    Strategic Importance: Accounting for 10 questions, Forensic Medicine and Toxicology is a lower-weightage subject in terms of volume. However, its questions are often direct, factual, and less complex than those in major clinical subjects. This makes FMT a high-scoring area and an important subject for rank differentiation. A well-prepared candidate can secure most of these 40 marks with targeted effort.  

    Detailed Topics: The syllabus combines the application of medical knowledge to legal matters with the study of poisons.

    • Forensic Medicine:
      • Introduction and Legal Procedures: Indian legal system, courts, inquests, types of evidence, and procedures for medical witnesses.  
      • Medical Jurisprudence and Ethics: Legal responsibilities of a doctor, medical negligence, consent (including different types), professional misconduct, and the Indian Medical Council (now NMC) regulations.  
      • Identity: Identification of living and dead individuals (age, sex, race, stature) and the role of fingerprints, DNA fingerprinting, and odontology.  
      • Thanatology: The study of death, including its definition, modes, signs of death (immediate, early, late), and estimation of the time since death (postmortem interval).  
      • Autopsy (Postmortem Examination): Objectives, types, procedures, and medico-legal aspects of autopsy.  
      • Forensic Traumatology (Injuries): Classification of mechanical injuries (abrasions, contusions, lacerations, incised wounds, stab wounds), medico-legal aspects of injuries, and regional injuries. This also includes firearm injuries (ballistics), thermal injuries (burns and scalds), and injuries from electricity and lightning.  
      • Asphyxial Deaths: A high-yield area covering the mechanisms and findings in violent asphyxial deaths, including hanging, strangulation (ligature and manual), suffocation, and drowning.  
    • Sexual Jurisprudence: Examination of the victim and accused in cases of sexual offenses, signs of virginity, pregnancy, delivery, abortion (and the MTP Act), and medico-legal aspects of infant deaths and battered baby syndrome.  
    • Forensic Psychiatry: Civil and criminal responsibilities of a mentally ill person and concepts like lucid interval and true vs. feigned insanity.  
    • Toxicology:
      • General Toxicology: Principles of toxicology, diagnosis of poisoning in the living and dead, general management of poisoning, and duties of a doctor in cases of poisoning.  
      • Specific Poisons: This is a major section covering the sources, signs and symptoms, fatal dose, fatal period, postmortem findings, and medico-legal aspects of:
        • Corrosive poisons (Acids and Alkalis)
        • Irritant poisons: Metallic (Arsenic, Lead, Mercury) and Non-metallic (Phosphorus)
        • Neurotoxic (Cerebral) poisons: Alcohol, Opioids, Barbiturates, Deliriants (Dhatura)
        • Cardiac poisons (Digitalis, Aconite, Tobacco)
        • Spinal poisons (Strychnos nux-vomica)
        • Asphyxiants (Carbon monoxide, Cyanide)
        • Agricultural poisons (Organophosphates, Carbamates, Organochlorines).  

    Within the broad scope of FMT, examination questions show a distinct concentration in two key areas: Indian Penal Code (IPC) sections relevant to medical practice and the specific clinical presentations (toxidromes) of common poisons. The syllabus explicitly mentions “Medical Jurisprudence” and “Legal responsibilities,” indicating that the exam will test a doctor’s awareness of their legal obligations and the potential legal ramifications of their actions. Questions frequently test knowledge of specific IPC sections related to causing injury, hurt, or death by negligence. Similarly, in toxicology, the focus is on pattern recognition. A question will typically describe a classic clinical scenario—a farmer brought in with salivation, lacrimation, urination, defecation, and pinpoint pupils—and ask for the identity of the poison (organophosphate) or the specific antidote (atropine and pralidoxime). An efficient preparation strategy, therefore, involves creating a concise, high-yield summary of important IPC sections and their definitions. For toxicology, the most effective tool is a “Poison-Symptoms-Antidote” table that covers the most frequently tested substances. This targeted approach yields a higher return on investment than attempting to memorize the entire subject in exhaustive detail.  

     

    Section 10: Social and Preventive Medicine (SPM) / Community Medicine

     

    Strategic Importance: With 25 questions, Social and Preventive Medicine shares the top spot among para-clinical subjects with Pathology. It is a subject with a vast and diverse syllabus, often perceived as volatile due to its reliance on current statistics and program updates. However, its high weightage makes it an indispensable component of a successful preparation campaign. A strong performance in SPM can significantly boost a candidate’s overall rank.  

    Detailed Topics: The syllabus covers a wide range of topics related to public health, disease prevention, and healthcare delivery.

    • Concepts of Health and Disease: Definitions and dimensions of health, indicators of health (mortality, morbidity), concept of well-being, natural history of disease, and the levels of prevention (primordial, primary, secondary, tertiary).  
    • Epidemiology and Biostatistics:
      • Epidemiology: A cornerstone of SPM, this includes definitions, aims, and uses of epidemiology. It covers measures of disease frequency (incidence, prevalence), measures of association (relative risk, odds ratio), epidemiological study designs (descriptive, case-control, cohort, randomized controlled trials), concepts of bias and confounding, screening of disease (sensitivity, specificity, predictive values, ROC curve), and investigation of an epidemic.  
      • Biostatistics: Data types, measures of central tendency (mean, median, mode), measures of dispersion (range, standard deviation), normal distribution, sampling methods, and tests of statistical significance (chi-square test, t-test).  
    • Epidemiology of Diseases: The epidemiology of major communicable diseases (e.g., Tuberculosis, HIV/AIDS, Malaria, Dengue, Diarrheal diseases) and non-communicable diseases (e.g., Hypertension, Diabetes Mellitus, Cardiovascular diseases, Cancers, Obesity).  
    • National Health Programs of India: This is a very high-yield area requiring in-depth and updated knowledge of all major national health programs, including the National Tuberculosis Elimination Program (NTEP), National AIDS Control Program (NACP), National Vector Borne Disease Control Program (NVBDCP), National Program for Prevention and Control of Cancer, Diabetes, Cardiovascular Diseases & Stroke (NPCDCS), and the National Program for Control of Blindness and Visual Impairment (NPCBVI).  
    • Maternal and Child Health (MCH): Antenatal, intranatal, and postnatal care. Reproductive and Child Health (RCH) Program, Integrated Management of Neonatal and Childhood Illnesses (IMNCI) guidelines, and the Universal Immunization Program (UIP), including the detailed national immunization schedule.  
    • Nutrition and Health: Macronutrients and micronutrients, nutritional assessment methods, protein-energy malnutrition (PEM), vitamin and mineral deficiency disorders, and national nutritional programs (e.g., Mid-Day Meal Scheme, ICDS).  
    • Environment and Health: Concepts of safe and wholesome water, water purification methods, sanitation, solid waste disposal (including detailed biomedical waste management rules), air pollution, noise pollution, and medical entomology (vectors of diseases).  
    • Health Planning and Management: The structure of the healthcare delivery system in India (Sub-centre, Primary Health Centre (PHC), Community Health Centre (CHC)), functions of healthcare workers, major health committees in India (e.g., Bhore, Mudaliar, Kartar Singh), National Health Policy, and international health agencies (WHO, UNICEF).  
    • Social Sciences: Concepts of sociology, family, community, and their relation to health and disease.  

    The primary challenge in preparing for SPM lies in its “numbers and names” nature. The subject is heavily dependent on the memorization of specific, often-changing data points. This includes the latest health statistics from sources like the Sample Registration System (SRS) bulletin (e.g., Infant Mortality Rate, Maternal Mortality Ratio), specific incubation periods for diseases, precise details of vaccine dosages and schedules, and the names, years, and key recommendations of various government health committees and programs. Questions in SPM are frequently direct and factual, testing pure recall. For example, a question might ask for the current IMR of India, the components of the ORS formula, the color-coding for biomedical waste disposal as per the latest rules, or the population norms for establishing a PHC. This reliance on factual recall means that traditional study methods must be supplemented with active recall techniques. The use of flashcards for memorizing numbers and names, the creation of comprehensive summary tables for all national health programs, and regular, repeated revision of the latest statistics and program updates are critical. While conceptual understanding is important, success in SPM is often determined by the ability to accurately recall these high-yield facts under examination pressure.

     

    Part IV: The Clinical Domain: Detailed Syllabus

     

     

    Section 11: General Medicine and Allied Specialties (Dermatology, Venereology, Psychiatry)

     

    Strategic Importance: This consolidated block of subjects is the undisputed heavyweight of the NEET PG examination, accounting for a total of 45 questions. This represents 22.5% of the entire paper, making it the joint highest-weighted subject group. A comprehensive and deep understanding of General Medicine and its allied branches is absolutely essential for achieving a high score. The questions are designed to test diagnostic acumen, clinical reasoning, and management skills.  

    Detailed Topics:

    • General Medicine: This is the core of the section, covering a vast array of systemic diseases.
      • Cardiology: Ischemic Heart Disease (Angina, Myocardial Infarction), Heart Failure, Rheumatic Heart Disease and Valvular Lesions, Cardiomyopathies, Pericardial Diseases, Hypertension, and common Arrhythmias.  
      • Pulmonology: Bronchial Asthma, Chronic Obstructive Pulmonary Disease (COPD), Pneumonia, Tuberculosis, Pleural Effusion, Interstitial Lung Diseases (ILD), and Sleep Apnea.  
      • Gastroenterology and Hepatology: Peptic Ulcer Disease, Gastroesophageal Reflux Disease (GERD), Inflammatory Bowel Disease (Crohn’s and Ulcerative Colitis), GI Bleeding, Malabsorption Syndromes, Viral Hepatitis, Alcoholic Liver Disease, and Cirrhosis with its complications.  
      • Nephrology: Acute Kidney Injury (AKI), Chronic Kidney Disease (CKD), Glomerular diseases (Nephrotic and Nephritic syndromes), and common Electrolyte and Acid-Base Disorders.  
      • Neurology: Cerebrovascular Accidents (Stroke), Seizure disorders and Epilepsy, Meningitis and Encephalitis, common Headache syndromes, Parkinson’s Disease and other movement disorders, Guillain-Barre Syndrome (GBS), and Myasthenia Gravis.  
      • Endocrinology: Diabetes Mellitus (including diagnosis, management, and acute/chronic complications), disorders of the Thyroid, Parathyroid, Adrenal, and Pituitary glands.  
      • Hematology: A comprehensive study of Anemias, Acute and Chronic Leukemias, Lymphomas, Multiple Myeloma, and common Bleeding and Coagulation disorders.  
      • Rheumatology and Connective Tissue Disorders: Rheumatoid Arthritis, Systemic Lupus Erythematosus (SLE), Systemic Sclerosis, Spondyloarthropathies, and Vasculitis syndromes.  
      • Infectious Diseases: A critical section covering major tropical infections (Malaria, Dengue, Typhoid, Leishmaniasis), HIV/AIDS and its opportunistic infections, Sepsis, and Fever of Unknown Origin. It also includes principles of poisoning and envenomation (snake bites).  
    • Dermatology and Venereology: This section covers common skin diseases, including infections (bacterial, fungal, viral), infestations (scabies), papulosquamous disorders (Psoriasis, Lichen Planus), vesiculobullous disorders (Pemphigus, Pemphigoid), pigmentary disorders (Vitiligo), acne vulgaris, and leprosy. Venereology covers Sexually Transmitted Diseases (STDs) and their management.  
    • Psychiatry: This includes the classification of psychiatric disorders, Schizophrenia and other psychotic disorders, Mood disorders (Depression, Bipolar disorder), Anxiety disorders (Panic disorder, GAD, Phobias, OCD), substance use disorders, personality disorders, organic mental disorders, and principles of psychotherapy and psychopharmacology.  

    A predominant question format in General Medicine is the “Next Best Step in Management” question. This type of question presents a detailed clinical vignette with patient history, examination findings, and initial lab reports, and then asks for the most appropriate subsequent action. The options provided often include various diagnostic tests or therapeutic interventions. This format is designed to assess clinical decision-making in a realistic sequence. It tests not just the ability to arrive at a diagnosis, but the understanding of the logical, evidence-based pathway of patient care. For example, for a patient with suspected acute coronary syndrome, the options might include ordering a troponin level, administering aspirin, performing an ECG, or scheduling an echocardiogram. While all are relevant to the patient’s condition, the “next best step” is invariably performing an ECG, as it is the most rapid and crucial initial diagnostic test. To prepare for this, aspirants must move beyond simply learning about diseases and focus on studying the standard management algorithms and clinical guidelines for common conditions. The key is to shift the learning focus from “What is the disease?” to “What do I do next?”. This requires a deep understanding of the indications, contraindications, and sequence of diagnostic investigations and therapeutic interventions.

     

    Section 12: General Surgery and Allied Specialties (Orthopaedics, Anaesthesia, Radiodiagnosis)

     

    Strategic Importance: Mirroring General Medicine, this comprehensive block of surgical specialties also accounts for 45 questions, making it the joint highest-weighted group in the NEET PG exam. A strong command of fundamental surgical principles, common surgical pathologies, and the basics of allied branches is critical for a top-tier performance.  

    Detailed Topics:

    • General Surgery:
      • Basic Surgical Principles: A foundational area covering the metabolic response to injury, shock (hypovolemic, septic, cardiogenic), wound healing and management, surgical infections and asepsis, fluid and electrolyte balance, nutritional support for surgical patients, principles of blood transfusion, and surgical ethics.  
      • Systemic and Specialty Surgery:
        • GI Surgery: A major component covering diseases of the esophagus, stomach, and duodenum (including peptic ulcer disease and malignancies), small and large intestines (intestinal obstruction, appendicitis, inflammatory bowel disease, colorectal cancer), rectum and anal canal (hemorrhoids, fissures, fistulas), and hernias.  
        • Hepatobiliary-Pancreatic Surgery: Diseases of the liver (abscesses, tumors), gallbladder (cholelithiasis, cholecystitis), bile ducts, and pancreas (pancreatitis, tumors).  
        • Endocrine Surgery: Diseases of the thyroid, parathyroid, and adrenal glands, and breast diseases (benign and malignant).  
        • Urology: Common conditions of the kidney, ureter, bladder, prostate (benign prostatic hyperplasia, carcinoma), and testis.  
      • Trauma: A high-yield topic based on Advanced Trauma Life Support (ATLS) principles, covering the systematic management of a polytrauma patient, including head, chest, and abdominal trauma.  
      • Other Areas: Basic principles of cardiothoracic surgery, vascular surgery (arterial and venous disorders), neurosurgery, plastic surgery (burns, skin grafts), and transplantation.  
    • Orthopaedics: This section focuses on the musculoskeletal system.
      • Traumatology: Principles of fracture management, diagnosis and treatment of specific fractures and dislocations of the upper limb, lower limb, and spine. Management of the polytrauma patient is key.  
      • Non-Traumatic Orthopaedics: Bone and joint infections (osteomyelitis, septic arthritis, tuberculosis of spine/hip), bone tumors (benign and malignant), metabolic bone diseases (rickets, osteomalacia), arthritis (osteoarthritis, rheumatoid), congenital disorders (CDH, CTEV), cerebral palsy, and peripheral nerve injuries.  
    • Anaesthesia: This covers pre-anesthetic evaluation and medication, principles of general anesthesia (inhalational and intravenous agents) and regional anesthesia (spinal, epidural), anesthetic equipment, airway management, monitoring during anesthesia, and principles of Cardiopulmonary Resuscitation (CPR).  
    • Radiodiagnosis: This includes the basic principles and physics of X-ray, Computed Tomography (CT), Magnetic Resonance Imaging (MRI), and Ultrasonography (USG). It covers the radiological appearance of common pathologies in various organ systems, the use of contrast agents, and principles of radiation safety.  

    The subjects of Surgery and Orthopaedics are characterized by a high density of eponymous signs, clinical triads, and staging or classification systems. An analysis of question patterns shows that these concise, high-yield facts are tested with remarkable frequency. They represent a distillation of complex clinical information into a memorable and easily testable format, making them ideal for MCQs that assess pattern recognition. For example, questions frequently test knowledge of Charcot’s triad for acute cholangitis (fever, jaundice, right upper quadrant pain), Beck’s triad for cardiac tamponade (hypotension, muffled heart sounds, distended neck veins), the TNM (Tumor, Node, Metastasis) classification system for staging cancers, the Forrest classification for GI bleeding, or the Garden and Pauwels classifications for femoral neck fractures. A highly effective study strategy is to create dedicated notes, mind maps, or flashcards for all major clinical signs, triads, diagnostic criteria (e.g., Ranson’s criteria for pancreatitis), prognostic scoring systems (e.g., Glasgow Coma Scale), and anatomical or pathological classification systems. Committing these to memory is an efficient method for securing a significant number of marks in the surgical disciplines.

     

    Section 13: Obstetrics and Gynaecology (OBG)

     

    Strategic Importance: With a substantial allocation of 30 questions, Obstetrics and Gynaecology is the third most important clinical subject in the NEET PG examination. It is a comprehensive and well-defined specialty with numerous high-yield topics. A strong performance in OBG is crucial for a good overall rank.  

    Detailed Topics: The syllabus is logically divided into the two main branches of the specialty.

    • Obstetrics: This branch deals with pregnancy, childbirth, and the postpartum period.
      • Foundations: Anatomy of the female reproductive tract, physiology of conception, fertilization, implantation, and the profound maternal physiological changes that occur during pregnancy.  
      • Antenatal Care: Diagnosis of pregnancy, routine antenatal care, and preconception counseling.  
      • Normal Labor and Puerperium: The physiology and stages of normal labor, management of labor, and care during the puerperium (postpartum period), including lactation.  
      • High-Risk Pregnancy and Complications: A major focus area including medical disorders complicating pregnancy (e.g., heart disease, anemia), hypertensive disorders in pregnancy (preeclampsia, eclampsia), gestational diabetes mellitus (GDM), multiple pregnancies, intrauterine growth restriction (IUGR), and Rh-isoimmunization.  
      • Obstetric Emergencies: Antepartum hemorrhage (APH), including placenta previa and abruptio placentae, and postpartum hemorrhage (PPH) are critically important topics. Others include ectopic pregnancy and abortions.  
      • Operative Obstetrics: Abnormal lie and presentation (breech, transverse), instrumental delivery (forceps, vacuum), and Cesarean section.  
    • Gynaecology: This branch focuses on the health of the female reproductive system.
      • Common Gynaecological Problems: Disorders of the menstrual cycle (amenorrhea, abnormal uterine bleeding – AUB), pelvic inflammatory disease (PID), genital prolapse, and urinary fistulae.  
      • Reproductive Endocrinology and Infertility: Polycystic Ovarian Syndrome (PCOS), endometriosis, evaluation and management of infertility, and principles of contraception.  
      • Gynaecological Oncology: Benign and malignant tumors of the uterus (fibroids, endometrial cancer), cervix (cervical intraepithelial neoplasia – CIN, cervical cancer), and ovaries. Screening for genital malignancies is a key topic.  
      • General Topics: Normal and abnormal puberty, menopause and hormone replacement therapy (HRT), and developmental (Mullerian) anomalies of the genital tract.  

    Within the vast syllabus of Obstetrics, a significant proportion of examination questions are concentrated on the “complications of pregnancy.” The syllabus repeatedly highlights topics such as “high-risk pregnancies,” “complications in early pregnancy,” “antepartum haemorrhage,” and “postpartum hemorrhage”. This emphasis reflects the clinical reality that the primary role of an obstetrician is to ensure the safety of both mother and fetus by expertly managing deviations from a normal pregnancy. The exam prioritizes the knowledge required to recognize, diagnose, and initiate management for life-threatening obstetric emergencies. Therefore, aspirants must have the management protocols for conditions like severe pre-eclampsia/eclampsia, the differential diagnosis and management of APH (placenta previa vs. abruption), and the stepwise management of PPH (the “four Ts”: Tone, Trauma, Tissue, Thrombin) committed to memory. These are high-stakes, high-yield topics that are virtually guaranteed to be tested, and a candidate’s ability to answer them correctly demonstrates a readiness to handle critical clinical situations.  

     

    Section 14: Paediatrics

     

    Strategic Importance: Paediatrics is allotted 10 questions in the NEET PG exam. While this is a smaller volume compared to the major clinical subjects, Paediatrics is a crucial subject with significant conceptual overlap with other disciplines, including Obstetrics (Neonatology), Social & Preventive Medicine (Immunization, Nutrition), and General Medicine (systemic diseases).  

    Detailed Topics: The syllabus covers the healthcare of infants, children, and adolescents.

    • Growth and Development: This is a cornerstone of Paediatrics. It includes the assessment of normal growth using anthropometry and growth charts, the sequence and age-appropriateness of developmental milestones (gross motor, fine motor, language, social), and the evaluation of common problems like short stature and developmental delay.  
    • Neonatology: A very high-yield area covering the care of the normal newborn, management of low birth weight (preterm and IUGR) babies, neonatal resuscitation (NRP guidelines), neonatal jaundice, neonatal sepsis, birth asphyxia (perinatal asphyxia), and other common neonatal problems.  
    • Nutrition: This includes the principles of breastfeeding, complementary feeding, assessment of nutritional status, and the diagnosis and management of common nutritional disorders like Protein-Energy Malnutrition (PEM) and deficiencies of vitamins and minerals.  
    • Infections and Immunization: This section covers common childhood infections (viral and bacterial) and congenital infections (TORCH). A detailed and updated knowledge of the National Immunization Schedule is absolutely mandatory.  
    • Systemic Paediatrics: This covers the common pediatric disorders of various organ systems:
      • Respiratory: Croup, bronchiolitis, pneumonia.
      • Gastrointestinal: Acute diarrheal diseases and dehydration, malabsorption.
      • Cardiovascular: Congenital heart diseases (acyanotic and cyanotic, e.g., VSD, ASD, Tetralogy of Fallot) and Rheumatic Heart Disease.
      • Neurology: Seizure disorders in children, febrile seizures, CNS infections.
      • Renal: Nephrotic syndrome, acute post-streptococcal glomerulonephritis (APSGN).
      • Hematology: Common anemias in children, bleeding disorders.
    • Paediatric Emergencies: Management of common emergencies, including fluid and electrolyte disturbances.  
    • Genetics and Behavioral Disorders: Common genetic syndromes and behavioral issues like ADHD and autism spectrum disorder.  

    The unique core of Paediatrics, which differentiates it from adult medicine, is formed by the triad of “Growth, Development, and Immunization.” An analysis of the syllabus and past question patterns reveals that these three topics are tested with high frequency and consistency. The examination will rigorously assess a candidate’s ability to determine if a child’s growth parameters fall within the normal range, to identify developmental “red flags” that warrant further investigation, and to recall the precise details of the national immunization schedule. Questions on these topics are often direct and factual, offering a high probability of scoring if prepared well. Therefore, it is imperative for aspirants to memorize the age-specific developmental milestones across all four domains (gross motor, fine motor, social, language) and the complete immunization table, including the name of the vaccine, the disease it prevents, the dosage, the route of administration, and the recommended age and schedule. Mastery of this core triad is fundamental to success in the Paediatrics section.  

     

    Section 15: Ophthalmology

     

    Strategic Importance: Ophthalmology is a specialized clinical subject that accounts for 10 questions in the NEET PG examination. While the number of questions is modest, the subject is highly visual, well-contained, and has many high-yield topics, making it a scoring opportunity for the well-prepared candidate.  

    Detailed Topics: The syllabus covers the anatomy, physiology, and diseases of the eye and visual system.

    • Anatomy and Physiology of the Eye: A thorough understanding of the structure and function of all parts of the eye, including the orbit, eyelids, lacrimal apparatus, conjunctiva, cornea, sclera, uveal tract (iris, ciliary body, choroid), lens, vitreous, retina, and optic nerve.  
    • Clinical Methods and Optics: This includes visual acuity assessment, examination of the eye, principles of optics and refraction, and correction of refractive errors (myopia, hypermetropia, astigmatism).  
    • Diseases of the Eye:
      • Conjunctiva and Cornea: Conjunctivitis (infective and allergic), corneal ulcers, and keratoconus.  
      • Lens: A major topic focusing on the etiology, classification, clinical features, and management of Cataract.  
      • Uveal Tract: Uveitis (anterior, intermediate, posterior) and its systemic associations.  
      • Glaucoma: Another critical, high-yield topic covering the classification, pathophysiology, clinical features, investigation, and management of open-angle and angle-closure glaucoma.  
      • Retina: Diabetic retinopathy, hypertensive retinopathy, retinal detachment, retinitis pigmentosa, and vascular occlusions of the retina.  
    • Neuro-ophthalmology: This covers the visual pathway and its lesions, pupillary reflexes and abnormalities, and optic nerve disorders like optic neuritis and papilledema.  
    • Strabismus (Squint): Evaluation and management of concomitant and incomitant strabismus.  
    • Ocular Trauma and Therapeutics: Management of mechanical and chemical injuries to the eye, and principles of ocular pharmacology.  
    • Community Ophthalmology: This section links with SPM and covers major causes of blindness in India and national programs for their control, such as the NPCBVI.  

    Given the inherently visual nature of this specialty, a large proportion of Ophthalmology questions are image-based. The subject is fundamentally about recognizing visual signs and patterns, such as a “cherry-red spot” on the retina in central retinal artery occlusion, the specific morphology of a dendritic ulcer in herpes simplex keratitis, or the characteristic appearance of a mature cataract. The most effective way to assess this skill is by presenting a clinical photograph of the eye’s anterior or posterior segment and asking for the diagnosis, the underlying pathology, or the associated systemic condition. For instance, a question might show a fundus photograph of diabetic retinopathy and ask for its grade, or display an image of Kayser-Fleischer rings in the cornea and ask for the associated systemic disease (Wilson’s disease). Consequently, preparation for Ophthalmology must be intensely visual. Aspirants should prioritize the use of high-quality clinical atlases, image-based question banks, and video lectures that demonstrate these visual signs clearly. Success in this section often depends on the ability to rapidly and accurately interpret these clinical images.

     

    Section 16: Otorhinolaryngology (ENT)

     

    Strategic Importance: Otorhinolaryngology, commonly known as ENT, is also allotted 10 questions in the NEET PG exam. Similar to Ophthalmology, it is a specialized surgical branch with a well-defined and manageable syllabus. Its strong anatomical basis makes it a subject where systematic study can yield predictable returns.  

    Detailed Topics: The syllabus covers the diseases of the ear, nose and paranasal sinuses, pharynx, larynx, and related structures in the head and neck.

    • Anatomy and Physiology: A detailed and thorough understanding of the anatomy and physiology of the ear (external, middle, inner), nose and paranasal sinuses, pharynx (nasopharynx, oropharynx, hypopharynx), and larynx is the absolute foundation for this subject.  
    • Ear (Otology):
      • Diseases of the external ear (e.g., otitis externa).
      • Diseases of the middle ear, a very high-yield area, including Otitis Media (acute, chronic, with effusion), its complications (e.g., mastoiditis, labyrinthitis, intracranial abscesses), and Otosclerosis.  
      • Disorders of the inner ear, including hearing loss (conductive vs. sensorineural), Meniere’s disease, and benign paroxysmal positional vertigo (BPPV).  
      • Anatomy and disorders of the facial nerve.  
    • Nose and Paranasal Sinuses (Rhinology):
      • Disorders of the external nose and nasal septum (e.g., deviated nasal septum).
      • Rhinitis (allergic and non-allergic), nasal polyps, and Epistaxis (nosebleeds) and its management.  
      • Rhinosinusitis (acute and chronic) and its complications.  
      • Tumors of the nose and paranasal sinuses.  
    • Pharynx and Larynx (Laryngology):
      • Disorders of the pharynx, including tonsillitis, adenoid hypertrophy, and deep neck space infections.  
      • Disorders of the larynx, including laryngitis, vocal cord paralysis, and benign and malignant tumors of the larynx.  
    • Head and Neck: This includes the evaluation of neck masses, salivary gland disorders, and principles of management for head and neck cancers.  

    A critical analysis of the ENT syllabus and question patterns reveals that Anatomy is king. A disproportionately large number of questions in this subject are either direct tests of anatomical knowledge or require a strong anatomical foundation to answer correctly. The detailed syllabus itself begins with an extensive exploration of the osteology and soft tissue anatomy of the head and neck region. Understanding the complex three-dimensional relationships between structures is essential for comprehending the pathophysiology of diseases, the pathways of infection spread, and the surgical approaches to treatment. For example, a question might ask about the most likely intracranial complication of chronic suppurative otitis media, which requires knowing the anatomical relationship between the middle ear cavity and the temporal lobe of the brain (separated by the thin tegmen tympani). Another question might test the nerve supply of the larynx to explain the effects of a recurrent laryngeal nerve palsy. Therefore, the single most high-yield activity for preparing for the ENT section is a meticulous and thorough revision of Head and Neck anatomy. Mastering the boundaries, contents, and relationships of the various spaces and structures in this region is the key to unlocking a majority of the questions.  

     

    Part V: Synthesis and Strategic Recommendations

     

     

    Section 17: An Integrated Approach to Preparation

     

    The preceding sections have provided a detailed, subject-by-subject breakdown of the NEET PG syllabus. However, the modern examination trend is moving decisively away from testing subjects in isolation. A recurring theme across the analysis is the increasing prevalence of integrated, multi-disciplinary, clinical-vignette-based questions. The examination is designed to assess a candidate’s ability to synthesize knowledge from various domains to solve a single clinical problem, mirroring the reality of medical practice. Therefore, the most effective preparation strategy is one that breaks down the artificial silos between subjects and fosters an integrated understanding of medicine.  

    This integrated approach requires a conscious effort to connect concepts across the pre-clinical, para-clinical, and clinical spectrum. When studying a disease, the aspirant should actively trace its connections back to the foundational sciences. For instance:

    • Studying Diabetes Mellitus (General Medicine): This should not be an isolated topic. It must be actively linked to:
      • Physiology: The normal synthesis and action of insulin, and the function of glucose transporters.
      • Biochemistry: The pathways of glucose metabolism and the formation of ketone bodies.
      • Pharmacology: The mechanisms of action of insulin and various oral hypoglycemic agents.
      • Pathology: The microscopic changes in the pancreas, kidneys (Kimmelstiel-Wilson lesions), and retina.
      • Ophthalmology: The clinical features and classification of diabetic retinopathy.
    • Studying Myocardial Infarction (General Medicine): This clinical emergency should be studied in conjunction with:
      • Anatomy: The detailed anatomy of the coronary arteries and their territories of supply.
      • Physiology: The principles of cardiac muscle function and the interpretation of ECG changes.
      • Biochemistry: The role of cardiac enzymes (troponins) as biomarkers.
      • Pathology: The pathogenesis of atherosclerosis and the morphological changes in the myocardium post-infarction.
      • Pharmacology: The mechanisms of action of thrombolytics, antiplatelet agents, beta-blockers, and statins.

    By adopting this integrated learning model, candidates can develop a deeper, more resilient understanding of medicine that is better suited to the demands of the NEET PG examination. This approach transforms study from a process of memorizing disconnected facts into one of building a logical and interconnected web of knowledge.

    In conclusion, the NEET PG is a comprehensive and challenging examination that rigorously evaluates the entirety of the MBBS curriculum. It is not merely a test of 19 separate subjects but a holistic assessment of a candidate’s ability to think like a clinician and function as a competent and safe Indian Medical Graduate. Success is contingent upon a strategy that is data-driven, prioritizing subjects based on their weightage, and intellectually integrative, connecting concepts across disciplines. A preparation plan built upon the detailed syllabus and strategic analysis provided in this report will equip an aspirant with the knowledge, skills, and perspective required to excel in this critical examination.

  • MAHE MBBS Microbiology Practical Materials (Manipal University)

    Manipal University (KMC Managalore-MAHE) MBBS Microbiology Practical Materials

     

    Manipal-University MBBS MAHE Microbiology-Practical-Materials

    MBBS Microbiology Practical Materials 2019     Download

     

    IDENTIFICATION OF BACTERIA

    1. STAPHYLOCOCCUS

    Specimen: Localized pus from abscess.
    Direct smear: Shows pus cells with gram positive cocci in clusters.
    Culture: Plating done on
    Culture media
    Incubation
    After 24 hr
    Nutrient agar
    Incubated at 37⁰c for 24 hr
    Opaque golden yellow colonies
    Blood agar
    Opaque colonies with beta hemolysis
    Mac conkey
    Tiny pink colonies
    Biochemical tests
    Urease
    Positive
    Mannitol
    Acid+;no gas
    Smear from culture plate: gram positive cocci in clusters.
    Coagulase test positive: Staphylococcus aureus
    Antibiotic susceptibility pattern.
    Phage typing – for epidemiology study
    Other species:
    Staphylococcus epidermidis – Opaque white colonies; coagulase test negative;
    Mostly a commensal, but the most frequent organism isolated from infected indwelling prosthetic devices.
    Staphylococcus albus – Opaque white colonies; coagulase test negative;
    Opportunistic pathogens.
    Staphylococcus citreus – lemon yellow colonies; coagulase negative;
    Opportunistic pathogens
    Clinical Significance:
    Staphylococcus aureus-Food poisoning, Toxic shock syndrome, Staphylococcal Skin Scalded Syndrome,
    Pneumonia, Osteomyelitis, Skininfections, Meningitis, Acute bacterial endocarditis, UTI
    Staphylococcus epidermidis-Mostly a commensal, but the most frequent organism isolated from
    3
    infected indwelling prosthetic devices, causes UTI, sepsis from IV line Methicillin-resistant Staphylococcus aureus (MRSA)-These bacterial isolates are resistant to many antibiotics. In the community, most MRSA infections are skin infections. In medical facilities, MRSA causes life threatening blood stream infections, pneumonia and surgical site infections.
    CASE HISTORY- 1
    A group of six children under 8 years of age live in a semitropical country. Each of the children has several crusted weeping skin lesions of impetigo (pyoderma). The lesions are predominantly on the arms and faces. Which of the following microorganisms is a likely cause of the lesions?
    (A) Escherichia coli
    (B) Chlamydia trachomatis
    (C) Staphylococcus aureus
    (D) Streptococcus pneumoniae
    (E) Bacillus anthracis
    4
    2. ESCHERICHIA COLI
    Specimen: Mid stream urine specimen in sterile container from
    Patient X having fever with burning micturition.
    Direct smear: Grams smear shows pus cells with gram negative rods.
    Wet Mount : Centrifuged, Plenty of pus cells seen
    Culture : After Incubation at 37⁰c for 24 hr
    Culture media
    Cultural Charecteristics
    Nutrient agar
    Blood agar
    Mac conkey
    Non Mucoid, Convex, Greyish white colonies
    Greyish white Non Mucoid Colonies
    Pink, Lactose fermenting colonies
    Smear from colonies: Gram negative rods are seen
    Biochemical tests:
    Indole
    Positive
    Urease
    Negative
    Citrate
    Negative
    TSI
    A/A Gas+ no H2S
    MR
    Positive
    VP
    Negative
    Sugar reaction: Glucose, lactose, sucrose, maltose, mannitol, starch are fermented with acid and gas.
    Antibiotic susceptibility testing.
    Other tests: Agglutination with mono and polyvalent antisera to detect EPEC, ETECetc
    Clinical Significance: Urinary tract Infections, Pyogenic infections, Septicemia, neonatal Meningitis
    Diarrhea (Enteropathogenic, Enterotoxigenic, Entero hemorrhagic, Enteroinvasive, enteroaggregative).
    Extended Spectrum Beta Lactamses (ESBL) producing isolates are resistance to 3rd generation cephalosporins (Ceftazidime, Cefotaxime, Ceftriaxone, Cefpodoxime) and Monobactams (Aztreonam). These ESBLs are of clinical concern because they restrict therapeutic options causing treatment failures.
    5
    CASE HISTORY – 2
    A 20-year-old college student goes to the student health center because of dysuria, frequency, and urgency on urination for 24 hours. She has recently become sexually active. On urinalysis, many polymorphonuclear cells are seen. The most likely organism responsible for these symptoms and signs is
    (A) Staphylococcus aureus
    (B) Streptococcus agalactiae
    (C) Gardnerella vaginalis
    (D) Lactobacillus species
    (E) Escherichia coli
    6
    3. KLEBSIELLA
    Specimen: Urine from patient X who complaints of fever,
    Lower abdominal pain, increased frequency of micturition.
    Direct smear: Grams smear shows pus cells with gram negative rods.
    Wet Mount : Centrifuged, Plenty of pus cells seen
    Culture : After Incubation at 37⁰c for 24 hr
    Culture media
    Cultural Charecteristics
    Blood agar
    Greyish white Colonies
    Mac conkey
    Pink, mucoid Lactose fermenting colonies
    Smear from plate: Thick Gram negative bacilli seen, with some bacilli showing halo around it.
    Biochemical tests:
    Indole
    Negative
    Citrate
    Positive
    Urease
    Positive
    TSI
    H2S
    Oxidase
    Negative
    Catalase
    Positive
    MR
    Negative
    VP
    Positive
    Sugar reactions: Glucose, lactose, sucrose, maltose, mannitol, starch are fermented with acid and gas.
    Animal Pathogenicity Test Done- Mice intraperitoneal inoculation done & organisms were demonstrated in the peritoneal fluid
    Antibiotic Susceptibility Testing And Klebocin Typing
    Clinical Significance: Pneumonia caused by Klebsiella species frequently involves the necrotic destruction of alveolar spaces, formation of cavities, and the production of blood-tinged sputum. These bacteria also cause wound & soft-tissue infection, and UTIs. Extended Spectrum Beta Lactamses (ESBL) producing isolates are resistance to 3rd generation cephalosporins (Ceftazidime, Cefotaxime, Ceftriaxone, Cefpodoxime) and Monobactams (Aztreonam). ESBL can pose a intimidating challenge with limited therapeutic options.
    7
    CASE HISTORY – 3
    The patient is a 40 year-old male with multisystem failure secondary to bilaterial pneumonia. Three days before he Complained to physician with history fever, malaise, and vague respiratory symptoms. He was given amantadine for suspected influenza. The patients condition became progressively worse, with shortness of breath a fever to 40.5 0C, and he was admitted to an outside hospital 24 h prior to transfer to this hospital. A laboratory examination revealed liver and renal functions as normal. Therapy with Timentin (Ticarcillin + Clavulanic acid) and trimethoprim-sulfamethoxazole was begun. On admission, he underwent a bronchoscopic examination which revealed mildly inflamed airways containing thin, watery secretions. A Gram stain of bronchial washings was obtained which showed the presence of gram negative bacilli. On culturing in Nutrient agar, it showed mucoid grayish white colonies.
    1.) Escherichia coli
    2.) Pseudomonas aeruginosa
    3.) Klebsiella pneumonia
    4.) Streptococcus pneumonia
    5.) Mycoplasma pnemoniae
    8
    4. PSEUDOMONAS
    Specimen: Wound swab from patient X
    Direct smear: Gram staining shows plenty of pus cells with Gram negative bacilli seen.
    Culture: After Incubation @ 37⁰c for 24 hr
    Culture media
    Colony Characteristics
    Nutrient agar
    Opaque irregular colonies with earthy smell. Pseudomonas pyogenes produce green pigment
    Blood agar plate
    Opaque irregular colonies surrounded by zone of hemolysis.
    Macconkey agar
    Non lactose fermenting colonies
    Smear from colony: Gram negative bacilli.
    Hanging drop: Motile rods seen
    Biochemical Tests:
    Indole
    Negative
    Urease
    Negative
    Citrate
    Positive
    TSI
    k/no change No gas/no H2S
    Oxidase
    Positve
    Catalase
    Positive
    Sugar reaction: Glucose- is utilized oxidatively, form acid only;
    Lactose; sucrose; maltose; mannitol- not fermented
    Pyocin typing and Antibiotic susceptibility testing.
    Clinical Significance: Pneumonia (Cystic fibrosis patient, Immunocompromised), Burns wound infection, bed sore infection, Skin and soft tissue infection, Urinary tract infection, Malignant otitis externa, Corneal ulcer for contact lens wearer or following trauma, Endocarditis for iv drug users, Septicemia.
    Most common cause for nosocomial infection
    9
    CASEHISTORY – 4
    A 37-year-old firefighter suffers smoke inhalation and is hospitalized for ventilatory support. He has a severe cough and begins to expectorate purulent sputum. Gram stain of his sputum specimen shows numerous polymorphonuclear cells and numerous gram-negative rods. Sputum culture grows numerous gram-negative rods that are oxidase-positive. They grow well at 42 °C. On clear agar medium they produce a blue-green color in the agar. The agar where the blue-green color is located fluoresces when exposed to ultraviolet light. The organism causing the patient”™s infection is
    (A) Burkholderia cepacia
    (B) Klebsiella pneumoniae
    (C) Escherichia coli
    (D) Pseudomonas aeruginosa
    (E) Burkholderia pseudomallei
    10
    5. PROTEUS
    Specimen: Urine of patient suffering from Urinary tract infection
    Direct smear: Pus cells with gram negative bacilli
    Culture: Plating done on
    Culture media
    Incubation
    After 24 hr
    Nutrient agar
    Incubated at 37⁰c for 24 hr
    Tiny colonies with swarming growth
    Blood agar
    Mac conkey
    Pale tiny non lactose fermenting colonies
    Smear from colony : gram negative bacilli seen, exhibits Pleomorphism. Different morphological forms are seen in the same organisms.
    Biochemical test:
    P.mirabilis P.vulgaris
    Indole
    Negative
    Positive
    Urease
    Positive
    Positive
    Citrate
    Positive
    Positive
    TSI
    Alk/acid
    Gas+
    H2S+
    Alk/acid
    Gas+
    H2S+
    Catalase
    Positive
    Positive
    Oxidase
    Negative
    Negative
    Hanging drop:motile gram negative bacilli seen
    Sugar reactions:
    Glu
    Lactose
    Sucrose
    Maltose
    Mannitol
    Acid+
    Not fermented
    Not fermented
    Acid+
    Not fermented
    Gas+
    Gas+
    Antibiotic susceptibility pattern: Amoxicillin Ciprofloxacin Cotrimoxazole Erythromycin Nalidicic acid Nitrofurantoin
    Clinical significance: Urinary Calculi formation
    11
    CASE HISTORY- 5
    A 37-year-old woman with a history of urinary tract infections comes to the emergency room with burning on urination along with frequency and urgency. She says her urine smells like ammonia. The cause of her urinary tract infection is likely to be
    (A) Enterobacter aerogenes
    (B) Proteus mirabilis
    (C) Citrobacter freundii
    (D) Escherichia coli
    (E) Serratia marcescens
    12
    6. VIBRIO
    Specimen: Rice water stools from patient suffering from acute watery diarrhea.
    Direct smear: Gram staining shows Gram negative bacilli. Some are comma shaped.
    Hanging Drop Preparation : Darting motility seen
    Culture:Transport media- enrichment media such as alkaline water or
    Monsur”™s med media or cary blair media is used to preserve Sample for long periods.
    Media Colony nature – After Incubation @ 37⁰c for 24 hr
    Nutrient agar Circular transparent water drop colonies
    Mac conkey Circular transport non lactose fermenting colonies
    Special Media – Thiosulphate Citrate Bile salt sucrose Mdium (TCBS) – Yellow circular colonies
    Smear from colony: Gram negative bacilli, some are comma shaped ; Hanging drop: Motile rods seen.
    Biochemical test:
    Indole
    Positive
    Urease
    Negative
    Citrate
    Negative
    TSI
    Acid/acid;no gas;noH2S
    Oxidase
    Positive
    Catalase
    Positive
    Cholera red reaction
    Polymyxin sensitivity
    Positive
    Sugar reaction:
    Glucose
    Lactose
    Sucrose
    Maltose
    Mannitol
    Mannose
    Arabinose
    Acid+

    Acid+
    Acid+
    Acid+
    Acid+

    Other tests:
    High titre sera agglutination for O group + serotypes ( Ogawa, Inaba, Hikojima)
    Chick cell agglutination to differ the EI tor and classical.
    Antibiotic susceptibility testing.
    Clinical Significance: Severe Watery diarrhea (Classically with Rice water stools)
    13
    CASE HISTORY – 6
    An 18-year-old woman in rural Bangladesh develops profuse (8 L/d) diarrhea. She has no symptoms other than the diarrhea and the manifestations of the fluid and electrolyte loss caused by the diarrhea. The most likely cause of her diarrhea is
    (A) Campylobacter jejuni
    (B) Enterotoxigenic Escherichia coli
    (C) Salmonella Typhimurium
    (D) Vibrio cholerae
    (E) Shigella dysenteriae
    14
    7. Salmonella typhi
    Specimen : Blood sample from patient X suffering from high grade fever 5 days
    duration with vomiting and abdominal pain .patient has palpable spleen.
    Culture : Specimen inoculated immediately at bed side of patient in to Ox bile
    or BHI broth medium & incubated for 24 hrs at 37c.
    Culture media Incubation After 24 hr Nutrient agar Incubate at 37 c for 24 hrs Convex , greyish white, colonies Blood agar Greyish white, colonies Mac conkey agar Non lactose fermenting colonies Selective media : ï‚·Wilson blair medium ï‚·Salmonella Shigella agar —————–
    Smear From Colony: Gram Negative Bacilli seen.
    Hanging Drop: Motile rod seen.
    BIOCHEMICAL TESTS
    Organism Indole Urease Catalase Oxidase Tsi Citrate S.typhi Negative Negative Positive Negative K/A;No Gas; Speck of H2S+ Negative
    Organism Glucose Lactose Sucrose Maltose Mannitol Xylose Arabinose S.typhi Acid + ; no gas ——— ———- Acid + ; no gas Acid + ; no gas Acid + ; no gas —————–
    Antibiotic Susceptibility Testing :
    High Titre Sera Agglutination Test: Poly O, Typhi H, O9
    New Taxonomy : Salmonella enterica enterica typhi
    Clinical Significance : Enteric Fever, Step ladder fever, have soft Palpable spleen, may have rose
    spots. It may lead on to intestinal perforation, hemorrhage & circulatory
    collapse
    15
    CASE HISTORY – 7
    A 27-year-old woman is admitted to the hospital because of fever, with increasing anorexia, headache, weakness, and altered mental status of 2 days”™ duration. She works for an airline as a cabin attendant, flying between the Indian subcontinent and other places in Southeast Asia and the West Coast of the United States. Ten days prior to admission she had a diarrheal illness that lasted for about 36 hours. She has been constipated for the last 3 days. Her temperature is 39 °C, heart rate 68/min, blood pressure 120/80 mm Hg, and respirations 18/ min. She knows who she is and where she is but does not know the date. She is picking at the bedclothes. Rose spots are seen on the trunk. The remainder of the physical examination is normal. Blood cultures are done and an intravenous line is placed. The most likely cause of her illness is
    (A) Enterotoxigenic Escherichia coli (ETEC)
    (B) Shigella sonnei
    (C) Salmonella enterica subspecies enterica serotype Typhimurium (Salmonella Typhimurium)
    (D) Salmonella enterica subspecies enteric serotype Typhi (Salmonella Typhi)
    (E) Enteroinvasive Escherichia coli (EIEC)
    16
    8. Salmonella paratyphi A
    Specimen : Blood sample from patient x suffering from fever 5 days duration with vomiting and abdominal pain .patient has palpable spleen.
    Culture : specimen inoculated immediately at bed side of patient in to ox bile
    medium & incubated for 24 hrs at 37c.
    Culture media Incubation After 24 hr Nutrient agar Incubate at 37 c for 24 hrs Convex , greyish white, colonies Blood agar Greyish white, colonies Mac conkey agar Non lactose fermenting colonies Selective media : ï‚·Wilson blair medium ï‚·Salmonella Shigella agar —————–
    Smear From Colony: Gram Negative Bacilli Seen.
    Hanging Drop: Motile Rod Seen.
    Biochemical Tests
    Organism Indole Urease Catalase Oxidase TSI Citrate S.Paratyphi A Negative Negative Positive Negative K/A; Gas +; No H2s Negative
    Sugar Reactions :
    Organism Glucose Lactose Sucrose Maltose Mannitol Xylose Arabinose S.Paratyphi A Acid + ; Gas + ———– ———– Acid + ; Gas + Acid + ; Gas + ———— Acid + ; Gas +
    Antibiotic Susceptibility Testing :
    High Titre Sera Agglutination Test : Poly O, O2
    Clinical Significance: Paratyphoid Fever, even lead on to frank septicemia with supparative complications
    17
    9. Salmonella paratyphi B
    Specimen : Blood sample from patient X suffering from fever 5 days duration with
    vomiting and abdominal pain .patient has palpable spleen.
    Culture : Specimen inoculated immediately at bed side of patient in to Ox bile
    medium & incubated for 24 hrs at 37c.
    Culture media Incubation After 24 hr Nutrient agar Incubate at 37 c for 24 hrs Convex , greyish white, colonies Blood agar Greyish white, colonies Mac conkey agar Non lactose fermenting colonies Selective media : ï‚·Wilson blair medium ï‚·Salmonella Shigella agar —————–
    Smear From Colony: Gram Negative Bacilli seen.
    Hanging Drop: Motile rod seen.
    BIOCHEMICAL TESTS
    Organism Indole Urease Catalase Oxidase TSI Citrate S.Paratyphi B Negative Negative Positive Negative K/A; Gas +; H2s + Positive
    Sugar Reactions
    Organism Glucose Lactose Sucrose Maltose Mannitol Xylose Arabinose S.paratyphi B Acid + ; gas + ————– ———— Acid + ; gas + Acid + ; gas + Acid + ; gas + Acid + ; gas +
    Antibiotic Susceptibility Testing :
    High Titre Sera Agglutination Test: Poly O, O4
    Clinical Significance: Paratyphoid Fever, even lead on to frank septicemia with supparative complications
    18
    LIST OF BACTERIAL ORGANISMS FOR IDENTIFICATION
    1. Staphylococcus Aureus
    2. Escherichia coli
    3. Klebsiella
    4. Pseudomonas
    5. Proteus
    6. Vibrio
    7. Salmonella typhi
    8. Salmonella paratyphi A
    9. Salmonella paratyphi B
    19
    MYCOLOGY
    Mucor sp.
    Macroscopic: Colonies are very fast growing, cottony to fluffy, white, becoming dark-grey, with the development of sporangia.
    Microscopic:
    ï‚· Broad ,irregular, aseptate hyaline hyphae seen.
    ï‚· The sporangiophores are long, straight with irregular branching.
    ï‚· Sporangiospores enlarge at distal end into collemullae
    ï‚· Branching sporangiophores with collamulla supporting sporangia are filled with sporangiospores
    ï‚· The sporangia are globose
    ï‚· No rhizoids are seen
    Clinical Significance: Mainly in patients with uncontrolled diabetes or trauma can cause opportunistic, and often spreading infections known as mucormycosis.
    20
    Rhizopus sp.
    Macroscopic: Colonies growth is rapid, with cotton texture. Salt and Pepper appearance. Colony appearing white initially, turns grey to yellowish brown in time.
    Reverse is white to Pale.
    Microscopic:
    ï‚· Hyphae or non septate or sparsely septate.
    ï‚· Sporangiophore are unbranched.
    ï‚· Rhizoids present.
    ï‚· Collumullae hemispherical.
    ï‚· Apophyses absent.
    ï‚· Hyaline or brown coloured round to ovoid Sporangiospores.
    Clinical Significance:
    Caused by Diabetes and Immunosuppression, can cause Zygomycosis is an angio invasive disease. It can be of several types mucocutaneous, rhinocerebral, genitourinary, gastrointestinal, pulmonary, and disseminated infections.
    21
    Obverse Reverse
    Rhizopus Microscopic- Rhizoid Present
    Aspergillus fumigatus
    Macroscopic: Dark green velvety colonies are seen
    Reverse white to Tan
    Microscopic:
    ï‚· Broad, hyaline septate hyphae seen
    ï‚· Conidiophores are smooth walled and are light green or brown in colour
    ï‚· At the teriminal end of conidiophores, flask shaped vesicle is seen
    ï‚· Uniseriate phialides are present at the upper half (2/3rd ) of the vesicle
    ï‚· Each phialide bears a chain of conidia
    Clinical Significance:
    It can cause Allergic broncho pulmonary aspergillosis, Aspergilloma (fungal ball infection developing in a preexisting cavity), Invasive Pulmonary Aspergillosis. In immunocompromised can cause Disseminated Aspergillosis.
    22
    Conidial head of A. fumigatus (Note: uniseriate row of phialides on the upper two thirds of the vesicle).
    Culture of Aspergillus fumigatus. Obverse Reverse
    23
    Aspergillus niger
    Macroscopic : Coarse black granules present against creamy colony
    Reverse white to yellow.
    Microscopic:
    ï‚· Broad , hyaline septate hyphae
    ï‚· Conidiophores are wide with a brown tint in upper half
    ï‚· Vesicles are spherical
    ï‚· Phialides are biseriate
    ï‚· The conidia are globose and are jet black
    Clinical Significance:
    It causes aspergillus infection affecting otitis externa, (Swimmer”™s Ear) , a chronic local inflammation which is characterized by itching, pain, scaling.
    Culture of Aspergillus niger. Obverse Reverse
    a
    24
    Note: Conidial head of A. niger. conidial heads are biseriate, large, globose, dark brown, becoming radiate with the phialides borne on metulae.
    Aspergillus flavus
    Macroscopic: Yello to Yellow green colonies seen
    Reverse goldish to red brown.
    Microscopic:
    ï‚· Broad , hyaline septate hyphae
    ï‚· Conidiophores are thick walled , hyaline
    ï‚· Vesicles are large and globose
    ï‚· Phialides are biseriate and present over the entire surface of the vesicle
    ï‚· The conidia are unicellular & globose
    Clinical Significance:
    It occurs in immunocompromised host, can cause Allergic broncho pulmonary aspergillosis. Less commonly it causes Invasive Pulmonary Aspergillosis
    Culture of Aspergillus flavus. Obverse Reverse
    Conidial head of A. flavus.
    25
    Note: conidial heads with both uniseriate and biseriate arrangement of phialides may be present over the entire surface. Candida Species
    Macroscopic: Creamy white moist or pasty colonies
    Microscopic: Oval Gram Positive budding Yeast Cells, Pseudohyphae are constricted at the ends and remain attached like links of sausages. Hyaline are septate
    Clinical Significance:
    It most commonly involves mucous membranes oral cavity ( Oral Candidiasis), vulva and vagina ( Vulvo vaginal candidiasis). It also causes cutaneous candidiasis. Disseminated candidiasis is caused in immunocompromised individuals ( eg., HIV, Organ transplantation, Neoplastic debilitating patients)
    Germ Tube Test – It helps to differentiate Candida albicans from non albicans group Germ tube is atrue hyphal structure and therefore does not have constriction characterize of pseudohyphae. Formation of Germ tube is present in Candida albicans
    Culture of Candida albicans- Obverse ReversE
    26
    Candida- Microscopic- Budding Yeast Cells
    27
    OSPE
    A. CULTURE MEDIA
    1. Identify the culture media?
    2. What type of media is this?
    3. List two main ingredients of this medium?
    4. List four organisms grown in this media?
    5. How is this media sterilized?
    28
    A. CULTURE MEDIA
    1.Nutrient Agar
    2.Simple media (basal media)
    3.Peptone water, 1%Meat extract, 2% Agar
    4.
    a. Staphylococcus aureus,
    b. Pseudomonas aeruginosa
    c. Escherichia coli
    d. Klebsiella pneumoniae
    5.Autoclaving
    29
    B. CULTURE MEDIA
    1.Identify the culture media?
    2.What type of media is this?
    3.List two main ingredients of this medium?
    4.List four organisms grown in this media?
    5.How is this media sterilized?
    30
    B. CULTURE MEDIA
    1.Blood Agar
    2.Enriched media
    3. 5% blood and nutrient agar
    4.
    a. Streptococcus pyogenes,
    b. Streptococcus pneumonia,
    c. Neisseria spp,
    d. Vibrio cholera
    5.Medium is prepared by adding sterile blood to sterile nutrient agar that has been melted and cooled to 50ºC
    31
    C. CULTURE MEDIA
    1. Identify the culture media?
    2. What type of media is this?
    3. List two main ingredients of this medium?
    4. List four organisms grown in this media?
    5. How is this media sterilized?
    32
    C.CULTURE MEDIA
    1.Mac Conkey medium
    2.Differential media or Indicator medium
    3.Lactose, Peptone, Agar, Neutral red & Taurocholate.
    4.
    a) Pseudomonas aeruginosa
    b) Escherichia coli
    c) Klebsiella pneumoniae
    d) Salmonella typhi
    5.Autoclaving
    33
    1. INSTRUMENT
    1. Identify the jar?
    2. What is the use of this jar?
    3. List four organism which can be grown by using this jar?
    4. Enumerate two disease caused by these organisms?
    5. Chemical indicator used for verifying the required condition in the jar?
    34
    1. INSTRUMENT
    1. McIntosh and Filde”™s anaerobic jar
    2. For cultivation of anaerobic organism by achieving anaerobiosis.
    3.Clostridium tetani, C. perfringens, C. botulinum, C. septicum
    4. C. tetani causes Tetanus.
    C. perfringens causes gas gangrene.
    5.Reduced methylene blue, it remains colorless anaerobically but turns blue on exposure to oxygen.
    35
    2. INSTRUMENT
    1. Identify the given object
    2. What is it used for and what is the type of test done by using this object?
    3. What is the antigen used in the test?
    4. What is the disease diagnosed by the test done using this object?
    5. What is the causative agent of the disease diagnosed by using this object?
    36
    2.INSTRUMENT
    1.VDRL Rotator
    2.It is used for doing VDRL test.
    VDRL (Venereal Disease Research Laboratory) test is a slide flocculation test.
    3.Cardiolipin antigen
    4.Syphilis
    5.Treponema pallidum.
    37
    3. INSTRUMENT
    1. What is this instrument ?
    2. What are the instruments that can be
    sterilized using this ?
    3. What is the ideal temperature and pressure ?
    4. What is the Sterility check used ?
    5. What is this type of sterilization ?
    38
    3.INSTRUMENT
    1. Autoclave
    2. Dressing, instruments,laboratory ware, media and pharmaceutical products
    3. 121°C for 15 minutes at 15 lbs
    4. Spores of Bacillus stearothermophilus
    5. Steam under pressure
    39
    4. INSTRUMENT
    1. What is this instrument?
    2. What are the instruments to be sterilized?
    3. Ideal temperature and holding time?
    4. Sterility check used.
    5. What is this type of sterilization?
    .
    40
    4.INSTRUMENT
    1. Hot air oven
    2. Glassware, forceps, scissors, glass syringes,swabs and pharmaceutical products”™
    3. 160°C for 1 hour.
    4. Spores of nontoxigenicstrain of Clostridium tetani or Bacillus subtilus
    5. Dry heat sterilization
    41
    CASE 1
    A 23 year old female gives a 2 day H/O fever, frequency, dysuria and mild haematuria. She also complains of suprapubic pain, but there is no vaginal discharge. There is no relevant previous history and examination is unremarkable.
    1. What is the probable diagnosis?
    2. List four common organisms causing this infection
    3. What is the relevant microbiological investigation?
    4. Name the media
    5. Describe the colonies
    6. Gram stain of the organism isolated showed Gram negative bacilli
    Identify the organism from the given biochemical reactions?
    42
    CASE 1
    A 23 year old female gives a 2 day H/O fever, frequency, dysuria and mild haematuria. She also complains of suprapubic pain, but there is no vaginal discharge. There is no relevant previous history and examination is unremarkable.
    1. Urinary tract infection
    2. Proteus, Escherichia coli, Klebsiella, Staphylococcus saprophyticus
    3. Urine culture & sensitivity
    4. MacConkey agar.
    5. MacConkey agar- Lactose Fermenting colonies
    6. Gram stain of the organism isolated showed Gram negative bacilli
    Identify the organism from the given biochemical reactions?
    Indole: positive
    TSI: A/A with gas, no H2S
    Urease: Negative
    Citrate: Not utilized
    Sugars:
    Glucose- – Fermented with acid & gas production
    Lactose- – Fermented with acid & gas production
    Sucrose– Fermented with acid & gas production
    Maltose– Fermented with acid & gas production
    Mannitol– Fermented with acid & gas production
    The organism is identified as Escherichia coli.
    43
    CASE 2
    A 14 year old boy gives H/O fever, headache and abdominal pain for the past 10 days. O/E he is toxic with coated tongue and hepatosplenomegaly.
    1. List two infective causes of fever?
    2. Write the relevant microbiological tests done to detect Enteric fever?
    3. Identify the given diagnostic test.
    4. Name the antigens used
    5. What is significant titre?
    44
    CASE 2
    A 14 year old boy gives H/O fever, headache and abdominal pain for the past 10 days. O/E he is toxic with coated tongue and hepatosplenomegaly.
    1.Enteric fever,Tuberculosis,Malaria.
    2.Blood Culture,Widal test,Stool Culture and Urine Culture.
    3.Widal test- Tube agglutination test.
    4. O antigen
    H antigen
    AH antigen
    BH antigen
    5.O agglutinin – 1:100 dilution or more.
    H agglutinin- 1:200 dilution or more.
    45
    CASE 3
    A 35 year old male with burns over both the arms gives an H/O discharge of pus from the wound.
    1. Name three bacteria commonly isolated from burns wound infection?
    2. What is the relevant microbiological investigation required in this case?
    3. Name the media?
    4. Describe the colonies?
    5. Gram stain of the organism isolated showed Gram negative bacilli
    Identify the organism from the given biochemical reactions
    46
    CASE 3
    A 35 year old male with burns over both the arms gives an H/O discharge of pus from the wound.
    1. Pseudomonas aeruginosa
    Staphylococcus aureus
    Proteus species
    2. Pus for culture and sensitivity.
    3. Nutrient Agar
    Mac Conkey agar
    4. Nutrient agar – Greenish pigmented colonies
    Mac Conkey agar – Non lactose fermenting colonies.
    5. Indole- Negative
    TSI- K/K
    Urease- Negative
    Citrate- Utilised
    Sugars- Glucose: no acid, no gas
    Lactose : no acid, no gas
    Sucrose: no acid, no gas
    Maltose: no acid, no gas
    Mannitol: no acid, no gas
    The organism is identified as Pseudomonas aeruginosa.
    47
    CASE 4
    A 40 year old male with a H/O discharge of pus from a wound in the leg. O/E a sinus is seen which is attached to the bone.
    1. Name three bacteria commonly isolated in osteomyelitis?
    2. What are the revelant microbiological investigation required to diagnose this condition?
    3. Name the media?
    4. Describe the colonies?
    5. Gram stain of the organism isolated showed Gram positive cocci
    Identify the organism from the given biochemical reactions?
    48
    CASE 4
    A 40 year old male with a H/O discharge of pus from a wound in the leg. O/E a sinus is seen which is attached to the bone.
    1. Staphylococcus aureus , Proteus, Pseudomonas aeruginosa.
    2. Pus for culture and sensitivity.
    3. Nutrient agar media.
    4. Golden yellow pigmented colonies.
    5. Urease : Positive.
    Mannitol is fermented with acid production without gas.
    Slide coagulase test : Positive.
    The organism is identified as Staphylococcus aureus.
    49
    CASE -5
    A 23 year old female gives a 2 day H/O fever, frequency, dysuria and mild haematuria. She also complains of suprapubic pain, but there is no vaginal discharge. There is no relevant previous history and examination is unremarkable.
    1. What is the probable diagnosis?
    2. List four common organisms causing this infection
    3. What is the relevant microbiological investigation?
    4. Name the media
    5. Describe the colonies
    6. Gram stain of the organism isolated showed Gram negative bacilli
    Identify the organism from the given biochemical reactions?
    50
    CASE -5
    A 23 year old female gives a 2 day H/O fever, frequency, dysuria and mild haematuria. She also complains of suprapubic pain, but there is no vaginal discharge. There is no relevant previous history and examination is unremarkable.
    1. Urinary tract infection
    2. Proteus, Escherichia coli, Klebsiella, Staphylococcus saprophyticus
    3. Urine culture & sensitivity
    4. MacConkey agar.
    5. MacConkey agar-Non Lactose Fermenting colonies
    6. Indole: negative
    TSI: K/A with gas, Abundant H2S
    Urease: positive
    Citrate: Not utilized
    Sugars:
    Glucose- – Fermented with acid & gas production
    Lactose- -Not Fermented
    Sucrose””Not Fermented
    Maltose””Not Fermented
    Mannitol””Not Fermented
    The organism is identified as Proteus mirabilis
    51
    SMEAR PREPARATION
    The preparation of a smear is required for many laboratory procedures, including the Gram-staining. The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure.
    Materials Required:
    Clean glass slides , Inoculating loops or needles , Normal saline or Sterile water , Glass marking pencil, Specimen ( may be Broth culture, Urine, Sputum , pus, swab,etc.,)
    Procedure:
    1. Take a clean, grease free glass slide. Wash the glass slide with fine sand soap , rinse it well, dry it thoroughly. Label your slide with the glass marking pencil.
    2. Place one loopful of bacterial growth in the center of a clean slide.
    3. If working from a solid medium, add one drop (and only one drop) of Normal Saline / Sterile water to the slide. If using a broth medium, do not add the water.
    4. Now, with the inoculating loop, mix the specimen with the Normal Saline/ Sterile water completely and spread the mixture out to cover about half of the total slide area.
    5. Place the slide on a slide warmer and wait for it to air dry.
    6. Dried smear is then fixed by passing it three times through the flame with the film facing downwards. . The smear is now ready for the staining procedure.
    52
    BIOMEDICAL WASTE MANAGEMENT
    1. What is biomedical / hospital wastes?
    Any wastes generated while providing healthcare,performing research &undertaking investigation or related procedures on human beings or animals in hospitals/laboratories or in any health care setup.
    2. What are the types of biomedical wastes?
    1.Infectious wastes – placenta,body fluids, laboratory samples, cultures, sharp wastes (forms only 10% of total waste)
    2.Non infectious hazardous wastes- chemicals,radioactive substances, pharmacological wastes.
    3. What are the objectives of biomedical waste management?
    ï‚· To prevent harm resulting from biomedical wastes.
    ï‚· To minimize waste volumes.
    ï‚· To retrieve reusable material.
    ï‚· To ensure safe &economical disposal.
    4. What are the colour coding & types of container for disposal of biomedical wastes?
    COLOUR CODING
    TYPES OF CONTAINER
    WASTE CATEGORY 1.YELLOW PLASTIC BAG HUMAN ANATOMICAL WASTES. DISCARDED MEDICINE. CYTOTOXIC DRUGS. 2.RED DISINFECTED CONTAINER PLASTIC BAG MICROBIOLOGICAL, BIOTECHNOLOGICAL WASTES. SOILED WASTES. SOLID WASTES. 3.BLUE / WHITE PUNCTURE PROOF CONTAINER WASTE SHARPS 3.BLACK PLASTIC BAG BIODEGRADABLE INCINERATOR ASH CHEMICAL WASTES HOUSEHOLD WASTES
    53
    5.What are the steps of biomedical wastes management?
    ï‚§ Reduction.
    ï‚§ Segregation at the point of generation of waste
    ï‚§ Storage.
    ï‚§ Transportation.
    ï‚§ Treatment.
    6.What are the methods of biomedical wastes treatment?(any 4)
     Chemical disinfections.
     Deep burial.
     Incineration.
     Autoclaving.
     Microwaving.
    54
    VENIPUNCTURE
    Procedure for Venipuncture:
    1. Clean your hands with soap and water or gel cleanser. Ask the patient to state his/her name. Determine if the test to be obtained has any special requirements.
    2. Explain the procedure to the patient. Position the arm for venipuncture; support the arm on a firm surface; the arm should be in a downward position. The median cubital and cephalic veins are most commonly used for venipuncture
    3. The patient can make a fist, but should not pump the hand open and closed. Apply tourniquet Palpate the vein. Release the tourniquet and assemble appropriate equipment.
    4. Wear gloves, Cleanse site with approved disinfectant. Allow the disinfectant to air-dry to avoid hemolysis of the specimen.
    5. Re-apply tourniquet about 3-4 inches above puncture site, donot palpate the vein, insert needle, bevel-side up, at about a 30° angle, and collect specimens
    6. Once sufficient blood has been collected, release the tourniquet BEFORE withdrawing the needle. Some guidelines suggest removing the tourniquet as soon as blood flow is established, and always before it has been in place for two minutes or more.
    7. Withdraw the needle gently and apply gentle pressure to the site with a clean gauze or dry cotton-wool ball. Ask the patient to hold the gauze or cotton wool in place, with the arm extended and raised. Ask the patient NOT to bend the arm, because doing so causes a haematoma.
    8. Apply direct pressure to stop bleeding at puncture site. After about 2 minutes, check the puncture site to verify that bleeding has stopped. Apply bandage if appropriate. Thank the patient for his/her cooperation.
    9. Label specimen(s) in the presence of the patient including all the information that is required by your facility.
    4.HANDWASHING METHOD
    55
    SEROLOGY
    Anti Streptolysin O (ASO) Test:
    Aim:
    To determine the presence of anti streptolysin O antibodies in the given serum
    Principle:
    It is a rapid latex agglutination test for the qualitative and semi-quantitative determination of anti-streptolysin-O antibodies (ASO) in serum. When the latex reagent is mixed with a serum containing ASO antibody, agglutination occurs. Sera having titers more than 200 IU/ml will be considered as positive.
    Procedure:
    1. Using a disposable pipette place one drop of each undiluted sample into its identified circle of the slide. Deliver one drop of positive and negative control into its identified circle.
    2. Mix the ASO latex reagent by gently shaking. Add one drop of reagent to each control and sample.
    3. Thoroughly mix each sample with reagent within the full area of the circle.
    4. Slowly rock the slide for exactly two (2) minutes and observe for agglutination under a high intensity light.
    5. Record results.
    Result / Interpretation:
    A test sample is considered to contain ASO antibodies in excess of 200 IU/ml when agglutination (clumping) is observed when compared to the result of the negative control (uniform suspension)
    56
    Rapid Plasma Reagin (RPR) Test:
    Aim:
    To detect IgM and IgG antibodies to lipoidal material released from damaged host cells as well as to lipoprotein-like material, and possibly cardiolipin released from the treponemes.
    Principle
    The rapid plasma reagin (RPR) test is a macroscopic, nontreponemal flocculation card test used to screen for syphilis.RPR antigen is mixed with unheated or heated (to inactivate complement) serum or with unheated plasma on a plastic-coated card.
    If antibodies are present, they combine with the lipid particles of the antigen, causing them to agglutinate. The charcoal particles coagglutinate with the antibodies and show up as black clumps against the white card. If antibodies are not present in the test serum, the test mixture is uniformly gray.
    Procedure:
    1. Using disposable serum dispensers or droppers, dispense one drop (0.05 ml) of serum or plasma sample onto a circle on the test card. Also add one drop of positive control and Negative control in the respective circle.
    2. Spread the sample smoothly across the circle area.
    3. After mixing the antigen solution by swirling, add one drop of the antigen suspension to each sample / control testing area. Do not stir or spread the antigen.
    4. Place the card on an VDRL rotator and cover to maintain humidity. Rotate at 100 ± 5 rpm for 8 minutes
    5. Immediately read results macroscopically in the “•wet”– state under a high intensity light source.
    Interpretation of RPR Test
    1. Non-reactive (NR)- smooth suspension, no clumping or slight roughness
    2. Reactive (R)- any degree of clumping
    If the test is negative, but the physician still suspects syphilis infection is present, the more specific treponemal tests (FTA-ABS, TPHA, TPI) should be performed because false positives can occur in RPR. False positives can occur in RPR. RPR is sensitive and used as screening test.
    57
    RHEUMATOID FACTOR
    Aim:
    To detect rheumatoid factor in the given serum qualitatively.
    Principle:
    Rheumatoid factors (RF) are antibodies directed against the Fc fragment of human and animal IgG, which acts as antigen.. The RF reagent is a suspension of polystyrene latex particles sensitized with specially prepared human IgG. The reagent is based on an immunological reaction between human IgG bound to biologically inert latex particles and rheumatoid factors in the test specimen.
    When serum containing rheumatoid factors is mixed with the latex reagent, visible agglutination occurs. The RF latex reagent sensitivity has been adjusted to detect a minimum of 8 IU/mL of rheumatoid factors according to the WHO International Standard without previous sample dilution
    Procedure:
    1. .Place one drop RF Positive and Negative Control in field 1 & 2. Using pipettes, place one drop of the undiluted specimens on successive fields.
    2. Gently resuspend the RF Latex Reagent and add one drop to each test field. Use pipette/Stir Stick to spread reaction mixture over entire test field.
    3. Rotate the slide manually or with a mechanical rotator at 80-100 rpm for 2 minutes and read immediately under direct light.
    4. Presence of agglutination of the latex particle is a positive result (see figure 1). Agglutination indicates a RF concentration of equal or more than 8 IU/ml. Sera with positive agglutination should be run again with the Quantitative Test.
    Result / Interpretation:
    Negative Result: A negative reaction is indicated by a uniform milky suspension with no agglutination as observed with the RF Negative Control.
    Positive Result: A positive reaction is indicated by any observable agglutination in the reaction mixture. The specimen reaction should be compared to the RF Negative and Positive Controls
    Positive result Signifies Rheumatoid Arthritis
    Positive
    58
    Negative
    C- REACTIVE PROTEIN
    Aim:
    To detect the presence of C-Reactive protein in the given serum
    Principle:
    CRP is one of the Acute phase protein, which is considered to be a sensitive indicator of inflammation. The principle of this test is based on the immunological reaction between CRP as an antigen and the corresponding antibody coated on the surface of biologically inert latex particles. The use of the CRP test to measure the effectiveness of therapy is of great clinical significance in cases such as rheumatoid arthritis and also other inflammation, bacterial and viral infection.
    Procedure:
    1. Gently shake the CRP latex vial to disperse and suspend latex particles. Positive and negative controls should be tested with each series of test.
    2. Using the disposable pipette provided, place one drop of test serum onto a circle on the slide. Use a separate disposable pipette for each test serum.
    3. Deliver one drop of CRP Latex to each circle that contains specimens on the slide. Spread the resulting mixture by using the paddle end of the pipette.
    4. Gently tilt and rotate slide by hand for two (2) minutes. Observe for macroscopic clumping using the indirect oblique light source. Compare the reaction of the test serum to the CRP positive and negative control sera.
    Result / Interpretation:
    Positive Result: Agglutination
    Negative Result: Smooth milky suspension
    Since negative results may be caused by CRP antigen excess, the test should be repeated using a diluted serum sample in case prozone effect is suspected.
    Positive result signifies that CRP >10mg/L.
    It is used ininfection or inflammation. Increased levels observed in Acute rheumatic fever and in Rheumatoid arthritis.
    59
    Negative
    Positive Positive Negative
    HEPATITIS B SURFACE ANTIGEN
    Aim:
    To detect the prescence of Hepatitis B Surface antigen in given serum or plasma.
    Principle:
    One step test for HBsAg utilizes the principle of Immunochromatography, a unique two site immunoassay on a membrane. As the test sample flows through the membrane assembly of the test device, the colored monoclonal anti-HBsAg-colloidal gold conjugate complexes with the HBsAg in the sample. This complex moves further on the membrane to the test region where it is immobilized by another monoclonal anti-HBsAg antiserum coated on the membrane leading to formation of a pink-purple colored band which confirms a positive test result. Absence of this colored band in the test region indicates a negative test result. The unreacted conjugate and unbound complex if any move further on the membrane and are subsequently immobilized by the anti-rabbit antiserum coated on the membrane at the control region, forming a pink-purple band. This control band serves to validate the test results.
    Procedure:
    1. Label the card with patient name or identification number.
    2. Use the disposable pipette, dispense about 2~3 drops sample in a vertical position into the sample well on the card.
    3. Wait for coloured bands to appear. Read within 15-20 minutes. Do not read results after 30 minutes.
    Result / Interpretation:
    Negative : Only one red line in the Control (C) area, with no coloured line in the Test (T) area indicates a negative result.
    Positive : Two red lines, one in the Test (T) area and one in the Control (C) area indicate a positive result.
    The test should be considered invalid if neither the test band nor the control band appear. Repeat
    the test with a new device.
    60
    Negative Positive
    Enzyme-linked immunosorbent assay (ELISA)
    Aim:
    To detect the presence of antibody against the specifeic antigen is present in the given serum.
    Principle:
    This testing method is a type of immunoassay. It is based on the principle that antibodies will bind to very specific antigens to form antigen-antibody complexes, and enzyme-linked antigens or antibodies can be used to detect and measure these complexes.
    Procedure:
    To detect or measure an antibody in a person’s blood, a known antigen is attached to a solid surface. A solution containing the patient sample is added. If the patient’s sample contains antibody, it will bind to the antigen. A second antibody (against human antibodies) that is labeled with an enzyme is then added. If the enzyme-linked antibody binds to human antibodies, the enzyme will create a detectable change that indicates the presence and amount of the antibody in the patient sample.
    Result / Interpretation:
    Color should develop in positive wells on addition of substrate within 30 minutes (yellow or orange, for pNPP or OPD, respectively). Absorbance may be read directly in a microplate reader (at 405 nm or 450 nm, for pNPP or OPD, respectively) or the reaction may be stopped with 50 μl per well of the appropriate stopping reagent and absorbance read later (at 405 nm or 492 nm, for pNPP or OPD, respectively).
    61
    62
    63
    64
    SPOTTERS
    NUTRIENT AGAR
     It is a simple medium.
     It is prepared by adding 2% agar to nutrient broth.
     It is sterilized by autoclaving.
    BLOOD AGAR
     It is an enriched medium.
     It is also a differential medium since the degree of hemolysis caused by hemolysin is assessed to differentiate among Gram positive colonies.
     It is used for growing fastidious organisms like Streptococci, Pneumococci, Hemophilus influenzae.
     It is prepared by adding sterile sheep blood to sterile nutrient agar that has been melted and cooled to 50 degree cent
    CHOCOLATE AGAR
    65
     It is an enriched medium.
     It is prepared by adding 10% sterile sheep blood to sterile molten nutrient agar at 75 degree centigrade.
     It is opaque and chocolate in colour.
     It is useful for the isolation of fastidious organisms like Hemophilus influenza, Neisseria meningitidis.
    TCBS MEDIUM
     It is a selective medium.
     It contains thiosulphate, citrate, bile salts, sucrose with bromothymol blue as an indicator.
     It is useful for isolation of vibrio cholerae which produces yellow coloured colonies due to fermentation of sucrose.
    MAC CONKEY AGAR
     It is a differential medium.
     It consists of peptone, lactose, agar, neutral red and sodium taurocholate.
     It is used to differentiate lactose fermenting colonies (pink) and non lactose fermenting colonies (colourless or pale).
     It is sterilized by autoclaving.
    66
    ROBERTSONS COOKED MEAT MEDIUM
     It is an anaerobic medium.
     It contains glucose broth with minced meat pieces with 1 cm layer of sterile liquid paraffin at the top.
     It is used for growing anaerobic organisms like Clostridium tetani.
    BACTERIOLOGICAL LOOP
     It is usually made of nichrome.
     It is used to transfer and streak clinical specimens onto culture medium.
     It is sterilized by heating red hot in flame.
    67
    MC INTOSH AND FILDES JAR
     It is used for anaerobic culture.
     It is made up of glass or stainless steel jar with a lid.
     The lid has an inlet and outlet.
     On the underside of the lid is the catalyst, consisting of alumina pellets coated with palladium.
     Reduced methylene blue is used as an indicator.
    PETRI DISH
     It is a shallow flat bottomed circular clear glass container with lid.
     It is usually 90mm in diameter.
    68
     Melted agar medium solidified in a petri dish provides a large surface area for the culture of bacteria.
     It is sterilized by hot air oven.
    DREYERS TUBE
     It is a narrow tube with a conical bottom.
     It is used for H antigen agglutination in Widal test.
     Loose fluffy cotton wool clumps seen in positive agglutination test.
    FELIX TUBE
     It is a short round bottom tube.
     It is used for O antigen agglutination in Widal test.
    69
     Disc like pattern with granular deposits seen at the bottom of the tube in positive agglutination test.
    STERILE SYRINGE
     It is used to collect specimens like blood, body fluids and pus.
     It should be used only once and to be disposed.
     It is sterilized by gamma radiation or ethylene oxide gas.
    UNIVERSAL CONTAINER
    70
     It is a screw capped glass bottle used to collect specimens like urine, blood etc.
     It has a capacity of 30 ml.
     It is sterilized by hot air oven.
    PASTEUR PIPETTE
     It is used to deliver solutions or reagents in various diagnostic procedures.
     It is sterilized by hot air oven
    71
    DURHAMS TUBE
     It is a small tube to detect gas formation in sugar fermentation reactions.
     Formation of air bubbles inside the tube indicates gas production.
    STERILE TEST TUBE
     It is used to collect specimens like blood, urine, body fluids.
     It is also used for keeping sterile swab.
     It is sterilized by hot air oven.
    STERILE SWAB
    72
     It is made up of absorbent cotton.
     It is used to collect specimens from throat, wounds and ear.
     It is used for making lawn culture for antibiotic sensitivity test.
     It is sterilized by hot air oven.
    MICROTITRE PLATE
     It is a polystyrene plate.
     It contains 96 wells (8 rows and 12 columns).
     Wells are coated with antigen or antibody.
     It is used for ELISA test.
    CONICAL FLASK
    73
     It is made up of borosilicate glass.
     It is of different capacities (eg., 250ml, 500ml, 1000ml).
     It is used as container for media.
     It is sterilized by hot air oven.
    VDRL ROTATOR
     It is used in VDRL test.
     It is used for uniform mixing of antigen and antibody.
     It rotates at 180 rotations per minute.
    74
    ANTIBIOGRAM
     Mueller Hinton agar is commonly used.
     Antibiotic discs are placed on Mueller Hinton agar and incubated at 37 º C for 18-24 hrs.
     Diameter of zone of inhibition is measured and it is interpreted as sensitive or resistant by comparing the zone size using the zone size interpretative chart.
    UREASE MEDIUM WITHOUT REACTION
     Christensens urease medium is used to determine the ability of an organism to produce urease.
     Phenol red is used as an indicator.
     Yellow colour indicates negative test, eg. Escherichia coli.
    75
    UREASE MEDIUM WITH REACTION
     Christensens urease medium is used to determine the ability of an organism to produce urease.
     Phenol red is used as an indicator.
     Pink colour indicates positive test. eg. Kelbsiella pneumoniae, Proteus vulgaris.
    CITRATE MEDIUM WITH REACTION
     Simmons citrate medium is used to determine the ability of an organism to utilise citrate as the sole source of carbon for its growth.
     Bromothymol blue is used as an indicator.
     Blue colour indicates positive test. eg., Klebsiella pneumoniae, Citrobacter.
    76
    CITRATE MEDIUM WITHOUT REACTION
     Simmons citrate medium is used to determine the ability of an organism to utilise citrate as sole source of carbon for its growth.
     Bromothymol blue is used as an indicator.
     Green colour indicates negative test. eg. Escherichia coli.
    TSI MEDIUM
     It is a triple sugar iron medium.
     It is a differential medium.
     It is in the form of slant and butt which is of equal size in the test tube.
     It contains three carbohydrates- 10% glucose, 1% sucrose, 1% lactose.
     It contains ferric salts to detect Hydrogen sulphide production.
     It is sterilized by autoclave.
    77
    INDOLE TEST – POSITIVE
     It is used to determine the ability of an organism to produce indole from tryptophan
     Formation of red coloured ring on adding kovacs reagent indicates positive test. eg. Escherichia coli, Proteus vulgaris.
    INDOLE TEST- NEGATIVE
     It is used to determine the ability of an organism to produce indole from tryptophan
     Absence of red coloured ring on adding kovacs reagent indicates negative test. eg. Klebsiella pneumoniae.
    78
    TSI MEDIUM- K/NO CHANGE
     It contains alkaline slant and alkaline butt ( Pink / Pink in colour).
     It indicates glucose, lactose and sucrose are not fermented .
     Eg., Pseudomonas aeruginosa.
    TSI MEDIUM- A/A
     It contains acid slant and acid butt (yellow / yellow in colour).
     It indicates fermentation of glucose, lactose and sucrose.
     Eg. Escherichia coli, Klebsiella pneumoniae.
    79
    TSI MEDIUM-K/A
     It contains alkaline slant and acid butt ( pink / yellow in colour).
     It indicates that glucose is only fermented.
     Eg. Shigella, Salmonella.
    TSI MEDIUM- K/A WITH H2S
     It contains alkaline slant and acid butt (pink/yellow in colour) with abundant hydrogen sulphide production.
     Hydrogen sulphide production is detected by blackening of the medium.
     Eg. Proteus vulgaris, Salmonella typhi para B.
    80
    LACTOSE FERMENTING COLONIES ON MAC CONKEY AGAR
     Lactose fermenting colonies are seen as pink coloured colonies in Mac Conkey agar.
     Eg. Escherichia coli, Klebsiella pneumoniae.
    NON LACTOSE FERMENTING COLONIES ON MAC CONKEY AGAR
     Colourless colonies are seen in Mac Conkey agar.
     Eg.Salmonella, Shigella, Proteus, Vibrio, Pseudomonas.
    81
    PSEUDOMONAS IN NUTRIENT AGAR
     Bluish green pigment produced in nutrient agar.
     Pigment diffuses into the medium.
     Pigments produced by Pseudomonas are pyocyanin( bluish green), pyoverdin (greenish yellow), pyorubin (red), and pyomelanin (brown).
    STAPHYLOCOCCUS IN NUTRIENT AGAR.
     Golden yellow pigmented colonies produced in nutrient agar.
     Pigment does not diffuse into the medium.
    82
     The pigment is enhanced by incorporation of `1% glycerol monoacetate or milk in the medium
     The pigment is considered to be carotenoid.
    CLOSTRIDIUM TETANI
     Slender Gram positive bacilli with spherical terminal spore having characteristic drum stick appearance.
     It is an anaerobe which causes tetanus.
    ACID FAST BACILLI
     Slender and pink coloured rod shaped bacilli seen against blue back ground.
    83
     Eg. Mycobacterium tuberculosis.
    SUGAR MEDIA WITHOUT REACTION
     It is a liquid medium used for sugar fermentation reactions.
     This medium contains peptone, sodium chloride, water, bromothymol blue and any one sugar (glucose, lactose, sucrose, maltose etc) and durhams tube.
     Due to non fermentation of sugars pH is not altered and colour remains blue.
    SUGAR MEDIA WITH ACID
     It is a liquid medium used for sugar fermentation reactions.
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     This medium contains peptone, sodium chloride, water, bromothymol blue and any one sugar (glucose, lactose, sucrose, maltose etc) and durhams tube.
     Due to fermentation of sugars pH is altered to acidic side and colour has changed from blue to yellow
    SUGAR MEDIA WITH ACID AND GAS
     It is a liquid biochemical medium used for sugar fermentation reactions.
     This medium contains peptone, sodium chloride, water, bromothymol blue and any one sugar (glucose, lactose, sucrose, maltose etc) and durhams tube.
     Due to fermentation of sugars pH is altered to acidic side and colour has changed from blue to yellow.
     Gas production is seen as air bubbles in durhams tube.
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    GRAM POSITIVE COCCI IN CLUSTERS
     Spherical in shape and violet in colour.
     Cocci are found in grape like clusters.
     Eg. Staphylococcus aureus.
    GRAM NEGATIVE BACILLI
     Pink in colour and rod shaped.
     Scattered in arrangement.
     Eg. Escherichia coli.
    CANDIDA ALBICANS
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     Gram positive budding yeast cells.
     Germ tube test is positive.
     It is an opportunistic fungi causing oral thrush.
    MUCOR
     It has non septate hyphae.
     Sphorangiophores are sympodially branched.
     Sporangia are globose and brown to grey in colour and columella are ovoid and contain sporangiospores.
     Rhizoids are absent.
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    RHIZOPUS
     Rhizoids with four to eight radial branches are seen.
     Sporangiophores are seen singly and are unbranched and may be yellowish brown to dark brown in colour.
     Sporangia may be gray beige to black in colour.
     Sporangia contains sporangiospores.
    TEANIA- SCOLEX
     Commonly called head.
     It is globular or quadrate in outline.
     It has four circular suckers.
     May or may not provided with rostellum and double row of hooklets.
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    TEANIA- PROGLOTTIDS
     It is an individual segment comprising the complete unit of tapeworm.
     According to its sexual maturity, a segment may be immature (reproductive organs are not differentiated) , mature ( reproductive organs appeared) and gravid (uterus filled with eggs).
     The common genital pore is situated marginally near the posterior end.
     The testes are numerous and the number of ovary is two.
    FASCIOLA HEPATICA.
     It is commonly known a liver fluke.
     It is large leaf shaped fluke.
     It contains two suckers- oral and ventral.
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    ENTEROBIUS VERMICULARIS.
     It is commonly known as pin worm, thread worm or seat worm.
     It is more or less spindle shaped and resembles a short piece of thread.
     In both male and female, a pair of cervical alae is present.
     Double- bulb oesophagus is a characteristic feature.
    HYDATID CYST
     It is the larval stage of Echinococcus granulosus.
     It contains many brood capsules and small protoscolices.
     Each protoscolex contains a scolex which represents the future head of the adult worm.
     The cyst wall of hydatid cyst consist of two layers- outer cuticular layer, which is a hyaline laminated membrane and inner germinal layer.
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     Hydatid cyst contains hydatid fluid which is secreted by germinal layer.
    ASCARIS LUMBRICOIDES
     Common name- Round worm.
     It is the largest intestinal nematode.
     Adult worms live in the lumen of small intestine.
     It is light brown or pink in colour.
     In shape it is rounded and tapers at both ends, the anterior end being thinner than posterior.
     Infection in man is known as ascariasis.
    TAPEWORM
     They are long , segmented and tape like hence known as tapeworm.
     They are flattened dorsoventrally.
     Sexes are not separate, that is hermaphrodite (monoecious).
     Adult worm contains head or scolex, neck and strobili consisting of proglottids.
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     Head end contains suckers, often with hooks.
     Alimentary canal and body cavity are absent.
    ENTEROBIUS VERMICULARIS
     It is commonly known as pinworm, thread worm, seat worm.
     Adult worms (gravid females) live in the caecum and vermiform appendix.
     It is small, more or less spindle-shaped and white in colour.
     In both male and female a pair of cervical alae is present at the anterior end.
     Double-bulb oesophagus is a characteristic feature of this nematode.
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    CERCARIA
     It is the final stage of larval development of trematodes in the mollusc. It possess a body and tail.
     According to the nature of tail different names are given. Eg Fork tailed (as in Schistosomes).
    TUBERCULIN SYRINGE
     Tuberculin Syringe is a small syringe with fine needle that hold upto one half to one cubic centimeter of fluid.
     It is used to administer antigen under the skin and perform tuberculin test.
     It is sterilized by gamma radiation.
    DEPRESSION SLIDE
     It is used for hanging drop preparation.
     It is used to demonstrate motility of the organism.
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    VDRL SLIDE
     It has 12 concavities.
     It is used to perform VDRL test to diagnose syphilis.
     It is a slide flocculation test.
    ASPERGILLUS FLAVUS
     It has hyaline septate hyphae.
     Conidiophores are thick walled, hyaline and coarsely roughened.
     Vesicles are large and globose.
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     They are produced over most of the vesicle.
     Conidia are unicellular and typically globose.
    ASPERGILLUS NIGER
     It has hyaline septate hyphae.
     Phialides are biseriate covering entire vesicle, form radial head.
     Conidiophores are wide hyaline changes into brown tint .
     Vesicles are spherical.
     Conidia are black in colour.
    ASPERGILLUS FUMIGATUS
     It has hyaline septate hyphae.
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     Phialides are single( uniseriate), usually cover upper half of the vesicle, parallel to the axis of stalk.
     Conidiophores are smooth walled, they may be light green or brown.
     Vesicle is flask shaped.
     Conidia are green in colour.
    PENCILLIUM
     It has hyaline septate hyphae.
     Conidiophores are branched with two rows of sterigmata bearing chains of spores, the appearance is like brush.

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