Download RGUHS B.Pharm Question Bank Pharmaceutical Microbiology And Biotechnology QP Bank

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Rajiv Gandhi University of Health Sciences, Karnataka, Bangalore

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Pharmaceutical Microbiology & Biotechnology

II Year B Pharm (RS4)

1. INTRODUCTION (2 marks)

1. Write contributions of Antony Van Leeuwenhoek.
2. Write contributions of Edward Jenner.

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3. Write contributions of Robert Koch.
4. Write contributions of Louis Pasteur.
5. Write contributions of Alexander Fleming.
6. Write the concept of spontaneous generation.
7. Write four pharmaceutical uses of microorganisms.

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8. State Koch’s postulates.
9. What is germs theory of diseases?
10. Write significance of swan necked Flask.
11. What is biogenesis?

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2. BACTERIOLOGY (10 marks)

1. Classify bacteria on the basis of nutritional requirements and add a note on raw materials used for preparation of culture media. (4+6)
2. Define and classify culture media. Mention salient feature of each media along with an example. (1+2+7)

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3. Draw an ultra structure of typical bacteria. Write composition and functions of its organelles. (3+7)
4. Classify bacteria on the basis of morphological features. Add a note on composition and functions of cell wall. (6+4)
5. Write briefly on various techniques used for identification of bacteria with emphasis on colony characters.
6. Differentiate between gram positive and Gram negative cell wall. Add a note on principle and procedure of Gram’s staining technique. (5+5)
7. Classify bacteria on the basis of oxygen, pH and temperature requirements. Add note on the effect of hyper and hypotonicity on bacteria. (2+2+2+4)

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8. Describe bacterial growth curve. Add a note on physical factors affecting growth of bacteria. (5+5)
9. Enlist methods used for total and viability counting bacteria. Describe any two methods of total counting. (4+6)
10. Explain continuous and synchronous growth techniques. Add a note on filter membrane method of counting bacteria. (5+5)
11. Mention methods used for identification of bacteria. Explain any four biochemical tests used for identification of bacteria. (2+8)
12. Classify bacteria on the basis of oxygen requirement. Explain any four efficient methods of cultivation of anaerobic bacteria. (2+8)

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13. Write in detail about agar plate methods of viability counting.

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